Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT2271

Experiment: PEG (Day 2) - Cage5;Mouse1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT2269 and BT2270 are separated by 128 nucleotidesBT2270 and BT2271 are separated by 61 nucleotidesBT2271 and BT2272 are separated by 102 nucleotidesBT2272 and BT2273 are separated by 34 nucleotides BT2269: BT2269 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 2,850,174 to 2,851,583 BT2269 BT2270: BT2270 - integral membrane protein (MarC family) (NCBI ptt file), at 2,851,712 to 2,852,371 BT2270 BT2271: BT2271 - haloacid dehalogenase-like hydrolase (NCBI ptt file), at 2,852,433 to 2,853,125 BT2271 BT2272: BT2272 - conserved hypothetical protein (NCBI ptt file), at 2,853,228 to 2,854,082 BT2272 BT2273: BT2273 - methyltransferase (NCBI ptt file), at 2,854,117 to 2,854,791 BT2273 Position (kb) 2852 2853 2854Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2851.497 kb on + strandat 2851.512 kb on - strandat 2851.516 kb on + strandat 2851.547 kb on - strandat 2851.578 kb on + strandat 2851.579 kb on - strandat 2851.579 kb on - strandat 2851.582 kb on - strandat 2851.603 kb on + strandat 2851.604 kb on - strandat 2851.616 kb on + strandat 2851.625 kb on - strandat 2851.667 kb on - strandat 2851.684 kb on + strandat 2851.685 kb on - strandat 2851.703 kb on + strandat 2851.713 kb on + strandat 2851.834 kb on + strand, within BT2270at 2851.835 kb on - strand, within BT2270at 2851.835 kb on - strand, within BT2270at 2851.928 kb on + strand, within BT2270at 2851.929 kb on - strand, within BT2270at 2851.930 kb on + strand, within BT2270at 2851.949 kb on - strand, within BT2270at 2852.023 kb on + strand, within BT2270at 2852.051 kb on - strand, within BT2270at 2852.082 kb on + strand, within BT2270at 2852.083 kb on - strand, within BT2270at 2852.099 kb on - strand, within BT2270at 2852.158 kb on + strand, within BT2270at 2852.211 kb on - strand, within BT2270at 2852.243 kb on - strand, within BT2270at 2852.243 kb on - strand, within BT2270at 2852.320 kb on + strandat 2852.321 kb on - strandat 2852.343 kb on - strandat 2852.365 kb on + strandat 2852.389 kb on + strandat 2852.398 kb on + strandat 2852.399 kb on - strandat 2852.414 kb on - strandat 2852.420 kb on + strandat 2852.420 kb on + strandat 2852.420 kb on + strandat 2852.421 kb on - strandat 2852.473 kb on + strandat 2852.473 kb on + strandat 2852.473 kb on + strandat 2852.473 kb on + strandat 2852.473 kb on + strandat 2852.474 kb on - strandat 2852.474 kb on - strandat 2852.516 kb on - strand, within BT2271at 2852.516 kb on - strand, within BT2271at 2852.754 kb on - strand, within BT2271at 2852.755 kb on + strand, within BT2271at 2852.756 kb on - strand, within BT2271at 2852.756 kb on - strand, within BT2271at 2852.756 kb on - strand, within BT2271at 2852.762 kb on + strand, within BT2271at 2852.950 kb on + strand, within BT2271at 2852.951 kb on - strand, within BT2271at 2852.993 kb on - strand, within BT2271at 2852.996 kb on - strand, within BT2271at 2852.996 kb on - strand, within BT2271at 2853.040 kb on + strand, within BT2271at 2853.042 kb on + strand, within BT2271at 2853.043 kb on - strand, within BT2271at 2853.138 kb on - strandat 2853.139 kb on + strandat 2853.139 kb on + strandat 2853.140 kb on - strandat 2853.141 kb on + strandat 2853.141 kb on + strandat 2853.143 kb on + strandat 2853.143 kb on + strandat 2853.143 kb on + strandat 2853.143 kb on + strandat 2853.144 kb on - strandat 2853.146 kb on + strandat 2853.173 kb on - strandat 2853.218 kb on + strandat 2853.219 kb on - strandat 2853.224 kb on + strandat 2853.225 kb on - strandat 2853.251 kb on - strandat 2853.284 kb on + strandat 2853.374 kb on - strand, within BT2272at 2853.396 kb on - strand, within BT2272at 2853.419 kb on - strand, within BT2272at 2853.549 kb on - strand, within BT2272at 2853.580 kb on - strand, within BT2272at 2853.668 kb on + strand, within BT2272at 2853.736 kb on + strand, within BT2272at 2853.736 kb on + strand, within BT2272at 2853.857 kb on + strand, within BT2272at 2853.962 kb on + strand, within BT2272at 2853.982 kb on - strand, within BT2272at 2854.082 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PEG (Day 2) - Cage5;Mouse1
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2,851,497 + -2.4
2,851,512 - -0.0
2,851,516 + -1.0
2,851,547 - -1.5
2,851,578 + -1.3
2,851,579 - -0.6
2,851,579 - -1.0
2,851,582 - +1.7
2,851,603 + -0.4
2,851,604 - -2.4
2,851,616 + -1.5
2,851,625 - +0.4
2,851,667 - -1.1
2,851,684 + -0.6
2,851,685 - -2.1
2,851,703 + -2.3
2,851,713 + -1.6
2,851,834 + BT2270 0.18 -1.8
2,851,835 - BT2270 0.19 -1.1
2,851,835 - BT2270 0.19 -0.3
2,851,928 + BT2270 0.33 +0.7
2,851,929 - BT2270 0.33 -1.1
2,851,930 + BT2270 0.33 -1.2
2,851,949 - BT2270 0.36 -0.4
2,852,023 + BT2270 0.47 +0.1
2,852,051 - BT2270 0.51 -2.0
2,852,082 + BT2270 0.56 -0.8
2,852,083 - BT2270 0.56 -0.8
2,852,099 - BT2270 0.59 -0.6
2,852,158 + BT2270 0.68 -1.6
2,852,211 - BT2270 0.76 +1.7
2,852,243 - BT2270 0.80 -0.3
2,852,243 - BT2270 0.80 -2.1
2,852,320 + -1.6
2,852,321 - -0.5
2,852,343 - -2.1
2,852,365 + -0.2
2,852,389 + +0.7
2,852,398 + -0.2
2,852,399 - -0.3
2,852,414 - -3.3
2,852,420 + -0.5
2,852,420 + +0.6
2,852,420 + -2.7
2,852,421 - -1.4
2,852,473 + -1.5
2,852,473 + -0.6
2,852,473 + -1.6
2,852,473 + -3.0
2,852,473 + -0.6
2,852,474 - -1.5
2,852,474 - +2.7
2,852,516 - BT2271 0.12 -0.3
2,852,516 - BT2271 0.12 -0.6
2,852,754 - BT2271 0.46 -1.5
2,852,755 + BT2271 0.46 -2.3
2,852,756 - BT2271 0.47 -0.3
2,852,756 - BT2271 0.47 +0.7
2,852,756 - BT2271 0.47 +0.7
2,852,762 + BT2271 0.47 -1.3
2,852,950 + BT2271 0.75 -1.9
2,852,951 - BT2271 0.75 -0.9
2,852,993 - BT2271 0.81 -1.5
2,852,996 - BT2271 0.81 -1.5
2,852,996 - BT2271 0.81 -1.8
2,853,040 + BT2271 0.88 -2.1
2,853,042 + BT2271 0.88 -0.3
2,853,043 - BT2271 0.88 -2.2
2,853,138 - -0.3
2,853,139 + +2.3
2,853,139 + -0.6
2,853,140 - -2.1
2,853,141 + +0.3
2,853,141 + -1.0
2,853,143 + -1.1
2,853,143 + +0.1
2,853,143 + -0.6
2,853,143 + -0.3
2,853,144 - -1.0
2,853,146 + +0.4
2,853,173 - -0.0
2,853,218 + -1.3
2,853,219 - -2.8
2,853,224 + -0.6
2,853,225 - +1.4
2,853,251 - -0.9
2,853,284 + +2.3
2,853,374 - BT2272 0.17 -1.1
2,853,396 - BT2272 0.20 -1.2
2,853,419 - BT2272 0.22 -1.4
2,853,549 - BT2272 0.38 -1.3
2,853,580 - BT2272 0.41 +0.1
2,853,668 + BT2272 0.51 +0.1
2,853,736 + BT2272 0.59 +1.1
2,853,736 + BT2272 0.59 -3.0
2,853,857 + BT2272 0.74 +0.7
2,853,962 + BT2272 0.86 +0.7
2,853,982 - BT2272 0.88 -0.3
2,854,082 + -1.8

Or see this region's nucleotide sequence