Experiment: Post-colonization (Day 14) - Cage7;Mouse3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2044 and BT2045 overlap by 33 nucleotides BT2045 and BT2046 are separated by 374 nucleotides BT2046 and BT2047 are separated by 37 nucleotides BT2047 and BT2048 are separated by 4 nucleotides
BT2044: BT2044 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 2,566,479 to 2,567,027
BT2044
BT2045: BT2045 - hypothetical protein (NCBI ptt file), at 2,566,995 to 2,567,309
BT2045
BT2046: BT2046 - putative cardiolipin synthetase (NCBI ptt file), at 2,567,684 to 2,568,880
BT2046
BT2047: BT2047 - thymidylate synthase (NCBI ptt file), at 2,568,918 to 2,569,712
BT2047
BT2048: BT2048 - dihydrofolate reductase (NCBI ptt file), at 2,569,717 to 2,570,211
BT2048
Position (kb)
2567
2568
2569 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2566.708 kb on - strand, within BT2044 at 2566.739 kb on + strand, within BT2044 at 2566.740 kb on - strand, within BT2044 at 2566.816 kb on + strand, within BT2044 at 2566.836 kb on + strand, within BT2044 at 2566.891 kb on - strand, within BT2044 at 2566.903 kb on + strand, within BT2044 at 2566.993 kb on - strand at 2567.228 kb on + strand, within BT2045 at 2567.228 kb on + strand, within BT2045 at 2567.232 kb on + strand, within BT2045 at 2567.338 kb on + strand at 2567.477 kb on - strand at 2567.569 kb on + strand at 2568.065 kb on + strand, within BT2046 at 2568.779 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Post-colonization (Day 14) - Cage7;Mouse3 remove 2,566,708 - BT2044 0.42 +0.6 2,566,739 + BT2044 0.47 +0.4 2,566,740 - BT2044 0.48 -1.0 2,566,816 + BT2044 0.61 -1.9 2,566,836 + BT2044 0.65 +0.9 2,566,891 - BT2044 0.75 +0.4 2,566,903 + BT2044 0.77 -0.7 2,566,993 - -1.2 2,567,228 + BT2045 0.74 -0.7 2,567,228 + BT2045 0.74 -1.7 2,567,232 + BT2045 0.75 -3.1 2,567,338 + -0.3 2,567,477 - -0.2 2,567,569 + +0.0 2,568,065 + BT2046 0.32 +1.6 2,568,779 - +1.6
Or see this region's nucleotide sequence