Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_400

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_399 and Pf6N2E2_400 are separated by 108 nucleotidesPf6N2E2_400 and Pf6N2E2_401 are separated by 135 nucleotides Pf6N2E2_399 - Iron siderophore sensor protein, at 467,733 to 468,656 _399 Pf6N2E2_400 - Ferrichrome-iron receptor, at 468,765 to 471,185 _400 Pf6N2E2_401 - Cyn operon transcriptional activator, at 471,321 to 472,220 _401 Position (kb) 468 469 470 471 472Strain fitness (log2 ratio) -2 -1 0 1 2at 467.902 kb on + strand, within Pf6N2E2_399at 467.903 kb on - strand, within Pf6N2E2_399at 467.903 kb on - strand, within Pf6N2E2_399at 468.030 kb on + strand, within Pf6N2E2_399at 468.030 kb on + strand, within Pf6N2E2_399at 468.031 kb on - strand, within Pf6N2E2_399at 468.031 kb on - strand, within Pf6N2E2_399at 468.674 kb on + strandat 468.693 kb on + strandat 468.936 kb on + strandat 468.936 kb on + strandat 468.937 kb on - strandat 468.937 kb on - strandat 468.990 kb on + strandat 469.118 kb on + strand, within Pf6N2E2_400at 469.119 kb on - strand, within Pf6N2E2_400at 469.161 kb on + strand, within Pf6N2E2_400at 469.161 kb on + strand, within Pf6N2E2_400at 469.248 kb on + strand, within Pf6N2E2_400at 469.248 kb on + strand, within Pf6N2E2_400at 469.248 kb on + strand, within Pf6N2E2_400at 469.249 kb on - strand, within Pf6N2E2_400at 469.249 kb on - strand, within Pf6N2E2_400at 469.249 kb on - strand, within Pf6N2E2_400at 469.587 kb on + strand, within Pf6N2E2_400at 469.588 kb on - strand, within Pf6N2E2_400at 469.588 kb on - strand, within Pf6N2E2_400at 469.825 kb on - strand, within Pf6N2E2_400at 469.825 kb on - strand, within Pf6N2E2_400at 469.896 kb on + strand, within Pf6N2E2_400at 469.896 kb on + strand, within Pf6N2E2_400at 469.897 kb on - strand, within Pf6N2E2_400at 469.897 kb on - strand, within Pf6N2E2_400at 469.897 kb on - strand, within Pf6N2E2_400at 469.935 kb on + strand, within Pf6N2E2_400at 469.935 kb on + strand, within Pf6N2E2_400at 469.935 kb on + strand, within Pf6N2E2_400at 469.935 kb on + strand, within Pf6N2E2_400at 469.935 kb on + strand, within Pf6N2E2_400at 469.935 kb on + strand, within Pf6N2E2_400at 469.935 kb on + strand, within Pf6N2E2_400at 469.935 kb on + strand, within Pf6N2E2_400at 469.936 kb on - strand, within Pf6N2E2_400at 469.936 kb on - strand, within Pf6N2E2_400at 469.936 kb on - strand, within Pf6N2E2_400at 469.936 kb on - strand, within Pf6N2E2_400at 469.936 kb on - strand, within Pf6N2E2_400at 469.936 kb on - strand, within Pf6N2E2_400at 469.936 kb on - strand, within Pf6N2E2_400at 469.936 kb on - strand, within Pf6N2E2_400at 469.936 kb on - strand, within Pf6N2E2_400at 469.936 kb on - strand, within Pf6N2E2_400at 469.957 kb on - strand, within Pf6N2E2_400at 469.984 kb on - strand, within Pf6N2E2_400at 470.004 kb on + strand, within Pf6N2E2_400at 470.004 kb on + strand, within Pf6N2E2_400at 470.004 kb on + strand, within Pf6N2E2_400at 470.005 kb on - strand, within Pf6N2E2_400at 470.005 kb on - strand, within Pf6N2E2_400at 470.005 kb on - strand, within Pf6N2E2_400at 470.026 kb on - strand, within Pf6N2E2_400at 470.037 kb on + strand, within Pf6N2E2_400at 470.037 kb on + strand, within Pf6N2E2_400at 470.037 kb on + strand, within Pf6N2E2_400at 470.037 kb on + strand, within Pf6N2E2_400at 470.037 kb on + strand, within Pf6N2E2_400at 470.037 kb on + strand, within Pf6N2E2_400at 470.037 kb on + strand, within Pf6N2E2_400at 470.037 kb on + strand, within Pf6N2E2_400at 470.037 kb on + strand, within Pf6N2E2_400at 470.038 kb on - strand, within Pf6N2E2_400at 470.038 kb on - strand, within Pf6N2E2_400at 470.038 kb on - strand, within Pf6N2E2_400at 470.038 kb on - strand, within Pf6N2E2_400at 470.038 kb on - strand, within Pf6N2E2_400at 470.038 kb on - strand, within Pf6N2E2_400at 470.038 kb on - strand, within Pf6N2E2_400at 470.043 kb on + strand, within Pf6N2E2_400at 470.080 kb on - strand, within Pf6N2E2_400at 470.180 kb on + strand, within Pf6N2E2_400at 470.247 kb on + strand, within Pf6N2E2_400at 470.248 kb on - strand, within Pf6N2E2_400at 470.460 kb on + strand, within Pf6N2E2_400at 470.461 kb on - strand, within Pf6N2E2_400at 470.461 kb on - strand, within Pf6N2E2_400at 470.487 kb on + strand, within Pf6N2E2_400at 470.514 kb on + strand, within Pf6N2E2_400at 470.515 kb on - strand, within Pf6N2E2_400at 470.527 kb on - strand, within Pf6N2E2_400at 470.630 kb on - strand, within Pf6N2E2_400at 470.719 kb on - strand, within Pf6N2E2_400at 470.719 kb on - strand, within Pf6N2E2_400at 470.721 kb on + strand, within Pf6N2E2_400at 470.994 kb on + strandat 470.995 kb on - strandat 471.105 kb on + strandat 471.105 kb on + strandat 471.105 kb on + strandat 471.105 kb on + strandat 471.106 kb on - strandat 471.106 kb on - strandat 471.106 kb on - strandat 471.129 kb on + strandat 471.152 kb on + strandat 471.170 kb on + strandat 471.233 kb on + strandat 471.233 kb on + strandat 471.234 kb on - strandat 471.234 kb on - strandat 471.248 kb on + strandat 471.249 kb on - strandat 471.314 kb on - strandat 471.322 kb on + strandat 471.322 kb on + strandat 471.322 kb on + strandat 471.323 kb on - strandat 471.323 kb on - strandat 471.323 kb on - strandat 471.492 kb on + strand, within Pf6N2E2_401at 471.493 kb on - strand, within Pf6N2E2_401at 471.493 kb on - strand, within Pf6N2E2_401at 471.501 kb on + strand, within Pf6N2E2_401at 471.502 kb on - strand, within Pf6N2E2_401at 471.590 kb on - strand, within Pf6N2E2_401at 471.590 kb on - strand, within Pf6N2E2_401at 471.755 kb on + strand, within Pf6N2E2_401at 471.756 kb on - strand, within Pf6N2E2_401at 471.759 kb on + strand, within Pf6N2E2_401at 471.854 kb on + strand, within Pf6N2E2_401at 471.855 kb on - strand, within Pf6N2E2_401at 471.898 kb on + strand, within Pf6N2E2_401at 471.898 kb on + strand, within Pf6N2E2_401at 471.898 kb on + strand, within Pf6N2E2_401at 471.899 kb on - strand, within Pf6N2E2_401at 471.899 kb on - strand, within Pf6N2E2_401at 471.994 kb on + strand, within Pf6N2E2_401at 472.009 kb on + strand, within Pf6N2E2_401at 472.018 kb on + strand, within Pf6N2E2_401at 472.018 kb on + strand, within Pf6N2E2_401at 472.018 kb on + strand, within Pf6N2E2_401at 472.019 kb on - strand, within Pf6N2E2_401at 472.019 kb on - strand, within Pf6N2E2_401at 472.019 kb on - strand, within Pf6N2E2_401

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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467,902 + Pf6N2E2_399 0.18 -2.0
467,903 - Pf6N2E2_399 0.18 -1.0
467,903 - Pf6N2E2_399 0.18 +1.0
468,030 + Pf6N2E2_399 0.32 +0.7
468,030 + Pf6N2E2_399 0.32 -0.1
468,031 - Pf6N2E2_399 0.32 -0.2
468,031 - Pf6N2E2_399 0.32 -0.3
468,674 + -0.2
468,693 + -0.8
468,936 + +0.1
468,936 + -1.0
468,937 - +0.2
468,937 - -1.4
468,990 + +0.7
469,118 + Pf6N2E2_400 0.15 +0.2
469,119 - Pf6N2E2_400 0.15 +0.6
469,161 + Pf6N2E2_400 0.16 -0.2
469,161 + Pf6N2E2_400 0.16 +0.6
469,248 + Pf6N2E2_400 0.20 +0.5
469,248 + Pf6N2E2_400 0.20 -0.1
469,248 + Pf6N2E2_400 0.20 +0.9
469,249 - Pf6N2E2_400 0.20 +1.0
469,249 - Pf6N2E2_400 0.20 +2.0
469,249 - Pf6N2E2_400 0.20 -0.5
469,587 + Pf6N2E2_400 0.34 +1.7
469,588 - Pf6N2E2_400 0.34 -0.4
469,588 - Pf6N2E2_400 0.34 +0.7
469,825 - Pf6N2E2_400 0.44 +0.0
469,825 - Pf6N2E2_400 0.44 -1.1
469,896 + Pf6N2E2_400 0.47 +1.0
469,896 + Pf6N2E2_400 0.47 +0.9
469,897 - Pf6N2E2_400 0.47 +0.0
469,897 - Pf6N2E2_400 0.47 +0.7
469,897 - Pf6N2E2_400 0.47 -1.1
469,935 + Pf6N2E2_400 0.48 +0.3
469,935 + Pf6N2E2_400 0.48 +0.7
469,935 + Pf6N2E2_400 0.48 +0.5
469,935 + Pf6N2E2_400 0.48 -0.3
469,935 + Pf6N2E2_400 0.48 +0.2
469,935 + Pf6N2E2_400 0.48 +0.8
469,935 + Pf6N2E2_400 0.48 +0.3
469,935 + Pf6N2E2_400 0.48 -0.3
469,936 - Pf6N2E2_400 0.48 -0.0
469,936 - Pf6N2E2_400 0.48 -0.2
469,936 - Pf6N2E2_400 0.48 +0.1
469,936 - Pf6N2E2_400 0.48 +0.3
469,936 - Pf6N2E2_400 0.48 +0.5
469,936 - Pf6N2E2_400 0.48 +0.2
469,936 - Pf6N2E2_400 0.48 +0.8
469,936 - Pf6N2E2_400 0.48 -1.0
469,936 - Pf6N2E2_400 0.48 +0.3
469,936 - Pf6N2E2_400 0.48 -0.2
469,957 - Pf6N2E2_400 0.49 -0.1
469,984 - Pf6N2E2_400 0.50 +0.7
470,004 + Pf6N2E2_400 0.51 -0.3
470,004 + Pf6N2E2_400 0.51 -0.0
470,004 + Pf6N2E2_400 0.51 +0.1
470,005 - Pf6N2E2_400 0.51 +1.5
470,005 - Pf6N2E2_400 0.51 -0.4
470,005 - Pf6N2E2_400 0.51 -0.2
470,026 - Pf6N2E2_400 0.52 +0.3
470,037 + Pf6N2E2_400 0.53 -0.6
470,037 + Pf6N2E2_400 0.53 +0.3
470,037 + Pf6N2E2_400 0.53 -0.7
470,037 + Pf6N2E2_400 0.53 -1.9
470,037 + Pf6N2E2_400 0.53 -0.1
470,037 + Pf6N2E2_400 0.53 +0.0
470,037 + Pf6N2E2_400 0.53 -0.3
470,037 + Pf6N2E2_400 0.53 -1.3
470,037 + Pf6N2E2_400 0.53 +1.3
470,038 - Pf6N2E2_400 0.53 +0.0
470,038 - Pf6N2E2_400 0.53 +0.4
470,038 - Pf6N2E2_400 0.53 +0.2
470,038 - Pf6N2E2_400 0.53 -0.3
470,038 - Pf6N2E2_400 0.53 -0.8
470,038 - Pf6N2E2_400 0.53 -0.3
470,038 - Pf6N2E2_400 0.53 -0.3
470,043 + Pf6N2E2_400 0.53 -0.0
470,080 - Pf6N2E2_400 0.54 -0.3
470,180 + Pf6N2E2_400 0.58 +1.2
470,247 + Pf6N2E2_400 0.61 +0.3
470,248 - Pf6N2E2_400 0.61 +1.4
470,460 + Pf6N2E2_400 0.70 -0.1
470,461 - Pf6N2E2_400 0.70 +0.9
470,461 - Pf6N2E2_400 0.70 +0.5
470,487 + Pf6N2E2_400 0.71 -0.4
470,514 + Pf6N2E2_400 0.72 +0.2
470,515 - Pf6N2E2_400 0.72 +0.3
470,527 - Pf6N2E2_400 0.73 -0.3
470,630 - Pf6N2E2_400 0.77 -0.6
470,719 - Pf6N2E2_400 0.81 +0.8
470,719 - Pf6N2E2_400 0.81 -0.7
470,721 + Pf6N2E2_400 0.81 +0.0
470,994 + +0.9
470,995 - -1.1
471,105 + -0.2
471,105 + +0.7
471,105 + +0.2
471,105 + -0.3
471,106 - -0.4
471,106 - -0.7
471,106 - -0.7
471,129 + -0.8
471,152 + +0.0
471,170 + -0.4
471,233 + -0.8
471,233 + +1.1
471,234 - -0.5
471,234 - -1.2
471,248 + +0.1
471,249 - -1.4
471,314 - +1.2
471,322 + +2.0
471,322 + -1.2
471,322 + -0.1
471,323 - +0.1
471,323 - -0.3
471,323 - +1.0
471,492 + Pf6N2E2_401 0.19 -0.9
471,493 - Pf6N2E2_401 0.19 -0.3
471,493 - Pf6N2E2_401 0.19 +0.8
471,501 + Pf6N2E2_401 0.20 -0.4
471,502 - Pf6N2E2_401 0.20 +2.0
471,590 - Pf6N2E2_401 0.30 -0.7
471,590 - Pf6N2E2_401 0.30 -0.2
471,755 + Pf6N2E2_401 0.48 +0.1
471,756 - Pf6N2E2_401 0.48 -0.8
471,759 + Pf6N2E2_401 0.49 +0.2
471,854 + Pf6N2E2_401 0.59 -1.4
471,855 - Pf6N2E2_401 0.59 -0.2
471,898 + Pf6N2E2_401 0.64 -1.0
471,898 + Pf6N2E2_401 0.64 +0.0
471,898 + Pf6N2E2_401 0.64 +0.2
471,899 - Pf6N2E2_401 0.64 -0.1
471,899 - Pf6N2E2_401 0.64 -0.7
471,994 + Pf6N2E2_401 0.75 -0.3
472,009 + Pf6N2E2_401 0.76 -0.8
472,018 + Pf6N2E2_401 0.77 -0.1
472,018 + Pf6N2E2_401 0.77 -0.7
472,018 + Pf6N2E2_401 0.77 +0.1
472,019 - Pf6N2E2_401 0.78 -0.1
472,019 - Pf6N2E2_401 0.78 -1.1
472,019 - Pf6N2E2_401 0.78 +0.2

Or see this region's nucleotide sequence