Experiment: PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_81 and Pf6N2E2_82 are separated by 249 nucleotides Pf6N2E2_82 and Pf6N2E2_83 are separated by 361 nucleotides
Pf6N2E2_81 - hypothetical protein, at 126,543 to 126,749
_81
Pf6N2E2_82 - hypothetical protein, at 126,999 to 127,151
_82
Pf6N2E2_83 - transcriptional regulator, AraC family, at 127,513 to 128,382
_83
Position (kb)
126
127
128 Strain fitness (log2 ratio)
-1
0
1
2 at 126.012 kb on + strand at 126.193 kb on + strand at 126.193 kb on + strand at 126.194 kb on - strand at 126.276 kb on - strand at 126.372 kb on - strand at 126.388 kb on - strand at 126.388 kb on - strand at 126.394 kb on - strand at 126.394 kb on - strand at 126.394 kb on - strand at 126.394 kb on - strand at 126.424 kb on + strand at 126.470 kb on + strand at 126.553 kb on - strand at 126.569 kb on - strand, within Pf6N2E2_81 at 126.569 kb on - strand, within Pf6N2E2_81 at 126.693 kb on - strand, within Pf6N2E2_81 at 126.747 kb on + strand at 126.748 kb on - strand at 126.748 kb on - strand at 126.815 kb on - strand at 126.815 kb on - strand at 126.815 kb on - strand at 126.881 kb on - strand at 126.964 kb on + strand at 126.964 kb on + strand at 126.964 kb on + strand at 126.964 kb on + strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.965 kb on - strand at 126.988 kb on + strand at 126.988 kb on + strand at 126.989 kb on - strand at 126.989 kb on - strand at 126.989 kb on - strand at 126.989 kb on - strand at 126.989 kb on - strand at 126.989 kb on - strand at 127.050 kb on - strand, within Pf6N2E2_82 at 127.093 kb on + strand, within Pf6N2E2_82 at 127.094 kb on - strand, within Pf6N2E2_82 at 127.094 kb on - strand, within Pf6N2E2_82 at 127.226 kb on + strand at 127.302 kb on - strand at 127.349 kb on - strand at 127.397 kb on + strand at 127.398 kb on - strand at 127.398 kb on - strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.412 kb on + strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.413 kb on - strand at 127.529 kb on + strand at 127.529 kb on + strand at 127.529 kb on + strand at 127.529 kb on + strand at 127.529 kb on + strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.530 kb on - strand at 127.568 kb on + strand at 127.569 kb on - strand at 127.580 kb on + strand at 127.580 kb on + strand at 127.581 kb on - strand at 127.581 kb on - strand at 127.649 kb on + strand, within Pf6N2E2_83 at 127.890 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.986 kb on + strand, within Pf6N2E2_83 at 127.987 kb on - strand, within Pf6N2E2_83 at 127.987 kb on - strand, within Pf6N2E2_83 at 127.987 kb on - strand, within Pf6N2E2_83 at 127.987 kb on - strand, within Pf6N2E2_83 at 128.140 kb on + strand, within Pf6N2E2_83
Per-strain Table
Position Strand Gene LocusTag Fraction PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x remove 126,012 + -0.4 126,193 + -0.3 126,193 + +2.0 126,194 - -0.4 126,276 - +0.1 126,372 - -0.6 126,388 - +0.5 126,388 - -0.1 126,394 - +0.5 126,394 - +0.9 126,394 - -0.8 126,394 - -0.2 126,424 + -1.3 126,470 + -0.5 126,553 - +0.4 126,569 - Pf6N2E2_81 0.13 -1.3 126,569 - Pf6N2E2_81 0.13 -0.4 126,693 - Pf6N2E2_81 0.72 -1.2 126,747 + -0.9 126,748 - +2.4 126,748 - +0.1 126,815 - -1.0 126,815 - -0.1 126,815 - -0.1 126,881 - +1.2 126,964 + +0.1 126,964 + +0.5 126,964 + -0.3 126,964 + -0.2 126,965 - +0.4 126,965 - +0.1 126,965 - +0.8 126,965 - +0.9 126,965 - -0.6 126,965 - -0.4 126,965 - -0.8 126,965 - +0.8 126,965 - -1.7 126,988 + -0.8 126,988 + +2.0 126,989 - +2.0 126,989 - -0.3 126,989 - +0.5 126,989 - +0.0 126,989 - +0.2 126,989 - -0.1 127,050 - Pf6N2E2_82 0.33 -0.7 127,093 + Pf6N2E2_82 0.61 -0.6 127,094 - Pf6N2E2_82 0.62 -0.9 127,094 - Pf6N2E2_82 0.62 -0.8 127,226 + +2.0 127,302 - +1.3 127,349 - -0.9 127,397 + -0.4 127,398 - -1.0 127,398 - -1.0 127,412 + +0.3 127,412 + +0.7 127,412 + +2.0 127,413 - +0.6 127,413 - +2.0 127,413 - -0.1 127,413 - +0.6 127,413 - +0.2 127,413 - +0.7 127,529 + +2.0 127,529 + -0.8 127,529 + -0.3 127,529 + +1.0 127,529 + -0.4 127,530 - -0.3 127,530 - +0.9 127,530 - -0.1 127,530 - -0.7 127,530 - +1.3 127,530 - -0.3 127,530 - -0.7 127,530 - -0.1 127,530 - +0.7 127,568 + -0.6 127,569 - +0.7 127,580 + -0.2 127,580 + -0.3 127,581 - -1.0 127,581 - -0.4 127,649 + Pf6N2E2_83 0.16 -0.1 127,890 + Pf6N2E2_83 0.43 -0.6 127,986 + Pf6N2E2_83 0.54 -0.1 127,986 + Pf6N2E2_83 0.54 +0.4 127,986 + Pf6N2E2_83 0.54 -0.9 127,986 + Pf6N2E2_83 0.54 +0.5 127,986 + Pf6N2E2_83 0.54 +0.2 127,986 + Pf6N2E2_83 0.54 +0.2 127,987 - Pf6N2E2_83 0.54 +1.0 127,987 - Pf6N2E2_83 0.54 -0.8 127,987 - Pf6N2E2_83 0.54 -0.9 127,987 - Pf6N2E2_83 0.54 -0.9 128,140 + Pf6N2E2_83 0.72 +1.2
Or see this region's nucleotide sequence