Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_593

Experiment: PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_591 and Pf6N2E2_592 overlap by 35 nucleotidesPf6N2E2_592 and Pf6N2E2_593 are separated by 86 nucleotidesPf6N2E2_593 and Pf6N2E2_594 are separated by 203 nucleotidesPf6N2E2_594 and Pf6N2E2_595 are separated by 197 nucleotides Pf6N2E2_591 - Streptothricin acetyltransferase, Streptomyces lavendulae type, at 668,446 to 669,060 _591 Pf6N2E2_592 - Transcriptional regulators, LysR family, at 669,026 to 669,919 _592 Pf6N2E2_593 - FIG00958166: hypothetical protein, at 670,006 to 670,269 _593 Pf6N2E2_594 - FMN reductase, NADPH-dependent, at 670,473 to 671,012 _594 Pf6N2E2_595 - Arsenical resistance operon repressor, at 671,210 to 671,560 _595 Position (kb) 670 671Strain fitness (log2 ratio) -2 -1 0 1 2at 669.121 kb on - strand, within Pf6N2E2_592at 669.302 kb on - strand, within Pf6N2E2_592at 669.344 kb on + strand, within Pf6N2E2_592at 669.345 kb on - strand, within Pf6N2E2_592at 669.345 kb on - strand, within Pf6N2E2_592at 669.382 kb on - strand, within Pf6N2E2_592at 669.382 kb on - strand, within Pf6N2E2_592at 669.382 kb on - strand, within Pf6N2E2_592at 669.634 kb on + strand, within Pf6N2E2_592at 669.635 kb on - strand, within Pf6N2E2_592at 669.657 kb on + strand, within Pf6N2E2_592at 669.705 kb on + strand, within Pf6N2E2_592at 669.912 kb on - strandat 669.997 kb on - strandat 670.015 kb on + strandat 670.143 kb on + strand, within Pf6N2E2_593at 670.143 kb on + strand, within Pf6N2E2_593at 670.144 kb on - strand, within Pf6N2E2_593at 670.144 kb on - strand, within Pf6N2E2_593at 670.144 kb on - strand, within Pf6N2E2_593at 670.145 kb on + strand, within Pf6N2E2_593at 670.145 kb on + strand, within Pf6N2E2_593at 670.145 kb on + strand, within Pf6N2E2_593at 670.146 kb on - strand, within Pf6N2E2_593at 670.146 kb on - strand, within Pf6N2E2_593at 670.146 kb on - strand, within Pf6N2E2_593at 670.259 kb on - strandat 670.275 kb on - strandat 670.343 kb on + strandat 670.344 kb on - strandat 670.412 kb on + strandat 670.412 kb on + strandat 670.412 kb on + strandat 670.412 kb on + strandat 670.412 kb on + strandat 670.412 kb on + strandat 670.413 kb on - strandat 670.413 kb on - strandat 670.414 kb on + strandat 670.414 kb on + strandat 670.415 kb on - strandat 670.415 kb on - strandat 670.415 kb on - strandat 670.418 kb on + strandat 670.418 kb on + strandat 670.418 kb on + strandat 670.418 kb on + strandat 670.418 kb on + strandat 670.419 kb on - strandat 670.460 kb on + strandat 670.460 kb on + strandat 670.460 kb on + strandat 670.461 kb on - strandat 670.461 kb on - strandat 670.461 kb on - strandat 670.467 kb on + strandat 670.596 kb on + strand, within Pf6N2E2_594at 670.597 kb on - strand, within Pf6N2E2_594at 670.597 kb on - strand, within Pf6N2E2_594at 670.597 kb on - strand, within Pf6N2E2_594at 670.597 kb on - strand, within Pf6N2E2_594at 670.597 kb on - strand, within Pf6N2E2_594at 670.598 kb on + strand, within Pf6N2E2_594at 670.598 kb on + strand, within Pf6N2E2_594at 670.599 kb on - strand, within Pf6N2E2_594at 670.615 kb on + strand, within Pf6N2E2_594at 670.615 kb on + strand, within Pf6N2E2_594at 670.615 kb on + strand, within Pf6N2E2_594at 670.615 kb on + strand, within Pf6N2E2_594at 670.632 kb on + strand, within Pf6N2E2_594at 670.632 kb on + strand, within Pf6N2E2_594at 670.632 kb on + strand, within Pf6N2E2_594at 670.692 kb on + strand, within Pf6N2E2_594at 670.692 kb on + strand, within Pf6N2E2_594at 670.692 kb on + strand, within Pf6N2E2_594at 670.692 kb on + strand, within Pf6N2E2_594at 670.693 kb on - strand, within Pf6N2E2_594at 670.968 kb on - strandat 670.968 kb on - strandat 671.031 kb on - strandat 671.173 kb on - strandat 671.174 kb on + strandat 671.175 kb on - strandat 671.175 kb on - strandat 671.188 kb on + strandat 671.206 kb on - strandat 671.206 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x
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669,121 - Pf6N2E2_592 0.11 -1.1
669,302 - Pf6N2E2_592 0.31 +0.0
669,344 + Pf6N2E2_592 0.36 -1.5
669,345 - Pf6N2E2_592 0.36 -0.2
669,345 - Pf6N2E2_592 0.36 -0.3
669,382 - Pf6N2E2_592 0.40 -0.5
669,382 - Pf6N2E2_592 0.40 +0.0
669,382 - Pf6N2E2_592 0.40 -0.3
669,634 + Pf6N2E2_592 0.68 +0.4
669,635 - Pf6N2E2_592 0.68 -0.7
669,657 + Pf6N2E2_592 0.71 -0.2
669,705 + Pf6N2E2_592 0.76 +0.6
669,912 - -1.0
669,997 - -0.0
670,015 + +0.3
670,143 + Pf6N2E2_593 0.52 -0.4
670,143 + Pf6N2E2_593 0.52 +0.1
670,144 - Pf6N2E2_593 0.52 -0.2
670,144 - Pf6N2E2_593 0.52 -0.5
670,144 - Pf6N2E2_593 0.52 -0.9
670,145 + Pf6N2E2_593 0.53 +0.5
670,145 + Pf6N2E2_593 0.53 -0.2
670,145 + Pf6N2E2_593 0.53 +1.7
670,146 - Pf6N2E2_593 0.53 -0.1
670,146 - Pf6N2E2_593 0.53 -0.9
670,146 - Pf6N2E2_593 0.53 -1.1
670,259 - +2.1
670,275 - +0.1
670,343 + -0.4
670,344 - -0.1
670,412 + +0.3
670,412 + +0.1
670,412 + -1.0
670,412 + -1.0
670,412 + +0.1
670,412 + -1.6
670,413 - -0.8
670,413 - +0.4
670,414 + -0.8
670,414 + +0.5
670,415 - -0.2
670,415 - +0.5
670,415 - -0.2
670,418 + -1.6
670,418 + -0.4
670,418 + -1.6
670,418 + -2.7
670,418 + -1.6
670,419 - -0.2
670,460 + +0.5
670,460 + +0.5
670,460 + +0.7
670,461 - -0.3
670,461 - +2.1
670,461 - +0.2
670,467 + -1.1
670,596 + Pf6N2E2_594 0.23 -0.0
670,597 - Pf6N2E2_594 0.23 -0.3
670,597 - Pf6N2E2_594 0.23 -0.9
670,597 - Pf6N2E2_594 0.23 +0.4
670,597 - Pf6N2E2_594 0.23 -1.8
670,597 - Pf6N2E2_594 0.23 +0.1
670,598 + Pf6N2E2_594 0.23 +0.9
670,598 + Pf6N2E2_594 0.23 -0.3
670,599 - Pf6N2E2_594 0.23 +0.1
670,615 + Pf6N2E2_594 0.26 -1.0
670,615 + Pf6N2E2_594 0.26 -0.9
670,615 + Pf6N2E2_594 0.26 -1.6
670,615 + Pf6N2E2_594 0.26 -0.1
670,632 + Pf6N2E2_594 0.29 +0.5
670,632 + Pf6N2E2_594 0.29 +0.6
670,632 + Pf6N2E2_594 0.29 -1.0
670,692 + Pf6N2E2_594 0.41 -0.7
670,692 + Pf6N2E2_594 0.41 +0.8
670,692 + Pf6N2E2_594 0.41 +0.2
670,692 + Pf6N2E2_594 0.41 -0.7
670,693 - Pf6N2E2_594 0.41 -0.2
670,968 - -2.0
670,968 - -1.3
671,031 - -0.4
671,173 - +0.1
671,174 + -0.3
671,175 - -1.3
671,175 - +0.4
671,188 + +1.0
671,206 - -0.4
671,206 - -0.4

Or see this region's nucleotide sequence