Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3501

Experiment: PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3500 and Pf6N2E2_3501 are separated by 3 nucleotidesPf6N2E2_3501 and Pf6N2E2_3502 overlap by 4 nucleotides Pf6N2E2_3500 - DNA-damage-inducible protein F, at 4,080,058 to 4,081,407 _3500 Pf6N2E2_3501 - hypothetical protein, at 4,081,411 to 4,082,208 _3501 Pf6N2E2_3502 - VgrG protein, at 4,082,205 to 4,083,251 _3502 Position (kb) 4081 4082 4083Strain fitness (log2 ratio) -1 0 1 2at 4080.512 kb on - strand, within Pf6N2E2_3500at 4080.512 kb on - strand, within Pf6N2E2_3500at 4080.521 kb on + strand, within Pf6N2E2_3500at 4080.521 kb on + strand, within Pf6N2E2_3500at 4081.058 kb on + strand, within Pf6N2E2_3500at 4081.058 kb on + strand, within Pf6N2E2_3500at 4081.058 kb on + strand, within Pf6N2E2_3500at 4081.058 kb on + strand, within Pf6N2E2_3500at 4081.059 kb on - strand, within Pf6N2E2_3500at 4081.059 kb on - strand, within Pf6N2E2_3500at 4081.126 kb on + strand, within Pf6N2E2_3500at 4081.132 kb on + strand, within Pf6N2E2_3500at 4081.132 kb on + strand, within Pf6N2E2_3500at 4081.132 kb on + strand, within Pf6N2E2_3500at 4081.133 kb on - strand, within Pf6N2E2_3500at 4081.133 kb on - strand, within Pf6N2E2_3500at 4081.133 kb on - strand, within Pf6N2E2_3500at 4081.133 kb on - strand, within Pf6N2E2_3500at 4081.141 kb on + strand, within Pf6N2E2_3500at 4081.177 kb on + strand, within Pf6N2E2_3500at 4081.177 kb on + strand, within Pf6N2E2_3500at 4081.177 kb on + strand, within Pf6N2E2_3500at 4081.177 kb on + strand, within Pf6N2E2_3500at 4081.178 kb on - strand, within Pf6N2E2_3500at 4081.178 kb on - strand, within Pf6N2E2_3500at 4081.178 kb on - strand, within Pf6N2E2_3500at 4081.302 kb on - strandat 4081.302 kb on - strandat 4081.405 kb on + strandat 4081.406 kb on - strandat 4081.552 kb on - strand, within Pf6N2E2_3501at 4081.598 kb on + strand, within Pf6N2E2_3501at 4081.598 kb on + strand, within Pf6N2E2_3501at 4081.598 kb on + strand, within Pf6N2E2_3501at 4081.599 kb on - strand, within Pf6N2E2_3501at 4081.599 kb on - strand, within Pf6N2E2_3501at 4081.599 kb on - strand, within Pf6N2E2_3501at 4081.602 kb on + strand, within Pf6N2E2_3501at 4081.602 kb on + strand, within Pf6N2E2_3501at 4081.602 kb on + strand, within Pf6N2E2_3501at 4081.602 kb on + strand, within Pf6N2E2_3501at 4081.602 kb on + strand, within Pf6N2E2_3501at 4081.602 kb on + strand, within Pf6N2E2_3501at 4081.603 kb on - strand, within Pf6N2E2_3501at 4081.603 kb on - strand, within Pf6N2E2_3501at 4081.850 kb on + strand, within Pf6N2E2_3501at 4081.851 kb on - strand, within Pf6N2E2_3501at 4081.851 kb on - strand, within Pf6N2E2_3501at 4081.851 kb on - strand, within Pf6N2E2_3501at 4081.862 kb on + strand, within Pf6N2E2_3501at 4081.862 kb on + strand, within Pf6N2E2_3501at 4081.863 kb on - strand, within Pf6N2E2_3501at 4081.871 kb on + strand, within Pf6N2E2_3501at 4081.871 kb on + strand, within Pf6N2E2_3501at 4081.871 kb on + strand, within Pf6N2E2_3501at 4081.872 kb on - strand, within Pf6N2E2_3501at 4081.872 kb on - strand, within Pf6N2E2_3501at 4081.872 kb on - strand, within Pf6N2E2_3501at 4081.872 kb on - strand, within Pf6N2E2_3501at 4081.872 kb on - strand, within Pf6N2E2_3501at 4081.874 kb on + strand, within Pf6N2E2_3501at 4081.874 kb on + strand, within Pf6N2E2_3501at 4081.874 kb on + strand, within Pf6N2E2_3501at 4081.875 kb on - strand, within Pf6N2E2_3501at 4081.875 kb on - strand, within Pf6N2E2_3501at 4081.875 kb on - strand, within Pf6N2E2_3501at 4081.875 kb on - strand, within Pf6N2E2_3501at 4081.875 kb on - strand, within Pf6N2E2_3501at 4081.875 kb on - strand, within Pf6N2E2_3501at 4081.875 kb on - strand, within Pf6N2E2_3501at 4081.875 kb on - strand, within Pf6N2E2_3501at 4082.155 kb on + strandat 4082.156 kb on - strandat 4082.403 kb on + strand, within Pf6N2E2_3502at 4082.403 kb on + strand, within Pf6N2E2_3502at 4082.403 kb on + strand, within Pf6N2E2_3502at 4082.404 kb on - strand, within Pf6N2E2_3502at 4082.404 kb on - strand, within Pf6N2E2_3502at 4082.463 kb on - strand, within Pf6N2E2_3502at 4082.573 kb on - strand, within Pf6N2E2_3502at 4082.573 kb on - strand, within Pf6N2E2_3502at 4082.573 kb on - strand, within Pf6N2E2_3502at 4082.735 kb on - strand, within Pf6N2E2_3502at 4082.936 kb on - strand, within Pf6N2E2_3502at 4082.973 kb on - strand, within Pf6N2E2_3502at 4083.042 kb on + strand, within Pf6N2E2_3502

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Per-strain Table

Position Strand Gene LocusTag Fraction PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x
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4,080,512 - Pf6N2E2_3500 0.34 +2.0
4,080,512 - Pf6N2E2_3500 0.34 +1.6
4,080,521 + Pf6N2E2_3500 0.34 -0.3
4,080,521 + Pf6N2E2_3500 0.34 +0.2
4,081,058 + Pf6N2E2_3500 0.74 -0.1
4,081,058 + Pf6N2E2_3500 0.74 +0.0
4,081,058 + Pf6N2E2_3500 0.74 -1.6
4,081,058 + Pf6N2E2_3500 0.74 -0.2
4,081,059 - Pf6N2E2_3500 0.74 -0.0
4,081,059 - Pf6N2E2_3500 0.74 -0.0
4,081,126 + Pf6N2E2_3500 0.79 -0.6
4,081,132 + Pf6N2E2_3500 0.80 +0.5
4,081,132 + Pf6N2E2_3500 0.80 +1.8
4,081,132 + Pf6N2E2_3500 0.80 +0.3
4,081,133 - Pf6N2E2_3500 0.80 -0.2
4,081,133 - Pf6N2E2_3500 0.80 -0.0
4,081,133 - Pf6N2E2_3500 0.80 +0.1
4,081,133 - Pf6N2E2_3500 0.80 -0.1
4,081,141 + Pf6N2E2_3500 0.80 +0.5
4,081,177 + Pf6N2E2_3500 0.83 +0.3
4,081,177 + Pf6N2E2_3500 0.83 +0.3
4,081,177 + Pf6N2E2_3500 0.83 -0.7
4,081,177 + Pf6N2E2_3500 0.83 -0.3
4,081,178 - Pf6N2E2_3500 0.83 -0.1
4,081,178 - Pf6N2E2_3500 0.83 +0.3
4,081,178 - Pf6N2E2_3500 0.83 -1.4
4,081,302 - -0.4
4,081,302 - -0.2
4,081,405 + -0.9
4,081,406 - +0.1
4,081,552 - Pf6N2E2_3501 0.18 -1.0
4,081,598 + Pf6N2E2_3501 0.23 -0.1
4,081,598 + Pf6N2E2_3501 0.23 +0.8
4,081,598 + Pf6N2E2_3501 0.23 +0.5
4,081,599 - Pf6N2E2_3501 0.24 +0.9
4,081,599 - Pf6N2E2_3501 0.24 +0.1
4,081,599 - Pf6N2E2_3501 0.24 +0.6
4,081,602 + Pf6N2E2_3501 0.24 +0.1
4,081,602 + Pf6N2E2_3501 0.24 +0.4
4,081,602 + Pf6N2E2_3501 0.24 -0.1
4,081,602 + Pf6N2E2_3501 0.24 +0.0
4,081,602 + Pf6N2E2_3501 0.24 +0.0
4,081,602 + Pf6N2E2_3501 0.24 -0.3
4,081,603 - Pf6N2E2_3501 0.24 -1.6
4,081,603 - Pf6N2E2_3501 0.24 -0.3
4,081,850 + Pf6N2E2_3501 0.55 +2.3
4,081,851 - Pf6N2E2_3501 0.55 +0.7
4,081,851 - Pf6N2E2_3501 0.55 -0.1
4,081,851 - Pf6N2E2_3501 0.55 -0.2
4,081,862 + Pf6N2E2_3501 0.57 +0.5
4,081,862 + Pf6N2E2_3501 0.57 -1.2
4,081,863 - Pf6N2E2_3501 0.57 +0.4
4,081,871 + Pf6N2E2_3501 0.58 +0.6
4,081,871 + Pf6N2E2_3501 0.58 -0.2
4,081,871 + Pf6N2E2_3501 0.58 +0.3
4,081,872 - Pf6N2E2_3501 0.58 -0.4
4,081,872 - Pf6N2E2_3501 0.58 +0.4
4,081,872 - Pf6N2E2_3501 0.58 -1.0
4,081,872 - Pf6N2E2_3501 0.58 +1.1
4,081,872 - Pf6N2E2_3501 0.58 +0.5
4,081,874 + Pf6N2E2_3501 0.58 +0.0
4,081,874 + Pf6N2E2_3501 0.58 +1.1
4,081,874 + Pf6N2E2_3501 0.58 +1.3
4,081,875 - Pf6N2E2_3501 0.58 -0.4
4,081,875 - Pf6N2E2_3501 0.58 +0.6
4,081,875 - Pf6N2E2_3501 0.58 +0.4
4,081,875 - Pf6N2E2_3501 0.58 -0.4
4,081,875 - Pf6N2E2_3501 0.58 -0.4
4,081,875 - Pf6N2E2_3501 0.58 +0.1
4,081,875 - Pf6N2E2_3501 0.58 -0.2
4,081,875 - Pf6N2E2_3501 0.58 +0.3
4,082,155 + +0.9
4,082,156 - -0.9
4,082,403 + Pf6N2E2_3502 0.19 +0.2
4,082,403 + Pf6N2E2_3502 0.19 -0.4
4,082,403 + Pf6N2E2_3502 0.19 -0.2
4,082,404 - Pf6N2E2_3502 0.19 +0.9
4,082,404 - Pf6N2E2_3502 0.19 +1.5
4,082,463 - Pf6N2E2_3502 0.25 +0.9
4,082,573 - Pf6N2E2_3502 0.35 -0.2
4,082,573 - Pf6N2E2_3502 0.35 -0.4
4,082,573 - Pf6N2E2_3502 0.35 -0.7
4,082,735 - Pf6N2E2_3502 0.51 +0.1
4,082,936 - Pf6N2E2_3502 0.70 -0.5
4,082,973 - Pf6N2E2_3502 0.73 -0.6
4,083,042 + Pf6N2E2_3502 0.80 -0.4

Or see this region's nucleotide sequence