Experiment: PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3500 and Pf6N2E2_3501 are separated by 3 nucleotides Pf6N2E2_3501 and Pf6N2E2_3502 overlap by 4 nucleotides
Pf6N2E2_3500 - DNA-damage-inducible protein F, at 4,080,058 to 4,081,407
_3500
Pf6N2E2_3501 - hypothetical protein, at 4,081,411 to 4,082,208
_3501
Pf6N2E2_3502 - VgrG protein, at 4,082,205 to 4,083,251
_3502
Position (kb)
4081
4082
4083 Strain fitness (log2 ratio)
-1
0
1
2 at 4080.512 kb on - strand, within Pf6N2E2_3500 at 4080.512 kb on - strand, within Pf6N2E2_3500 at 4080.521 kb on + strand, within Pf6N2E2_3500 at 4080.521 kb on + strand, within Pf6N2E2_3500 at 4081.058 kb on + strand, within Pf6N2E2_3500 at 4081.058 kb on + strand, within Pf6N2E2_3500 at 4081.058 kb on + strand, within Pf6N2E2_3500 at 4081.058 kb on + strand, within Pf6N2E2_3500 at 4081.059 kb on - strand, within Pf6N2E2_3500 at 4081.059 kb on - strand, within Pf6N2E2_3500 at 4081.126 kb on + strand, within Pf6N2E2_3500 at 4081.132 kb on + strand, within Pf6N2E2_3500 at 4081.132 kb on + strand, within Pf6N2E2_3500 at 4081.132 kb on + strand, within Pf6N2E2_3500 at 4081.133 kb on - strand, within Pf6N2E2_3500 at 4081.133 kb on - strand, within Pf6N2E2_3500 at 4081.133 kb on - strand, within Pf6N2E2_3500 at 4081.133 kb on - strand, within Pf6N2E2_3500 at 4081.141 kb on + strand, within Pf6N2E2_3500 at 4081.177 kb on + strand, within Pf6N2E2_3500 at 4081.177 kb on + strand, within Pf6N2E2_3500 at 4081.177 kb on + strand, within Pf6N2E2_3500 at 4081.177 kb on + strand, within Pf6N2E2_3500 at 4081.178 kb on - strand, within Pf6N2E2_3500 at 4081.178 kb on - strand, within Pf6N2E2_3500 at 4081.178 kb on - strand, within Pf6N2E2_3500 at 4081.302 kb on - strand at 4081.302 kb on - strand at 4081.405 kb on + strand at 4081.406 kb on - strand at 4081.552 kb on - strand, within Pf6N2E2_3501 at 4081.598 kb on + strand, within Pf6N2E2_3501 at 4081.598 kb on + strand, within Pf6N2E2_3501 at 4081.598 kb on + strand, within Pf6N2E2_3501 at 4081.599 kb on - strand, within Pf6N2E2_3501 at 4081.599 kb on - strand, within Pf6N2E2_3501 at 4081.599 kb on - strand, within Pf6N2E2_3501 at 4081.602 kb on + strand, within Pf6N2E2_3501 at 4081.602 kb on + strand, within Pf6N2E2_3501 at 4081.602 kb on + strand, within Pf6N2E2_3501 at 4081.602 kb on + strand, within Pf6N2E2_3501 at 4081.602 kb on + strand, within Pf6N2E2_3501 at 4081.602 kb on + strand, within Pf6N2E2_3501 at 4081.603 kb on - strand, within Pf6N2E2_3501 at 4081.603 kb on - strand, within Pf6N2E2_3501 at 4081.850 kb on + strand, within Pf6N2E2_3501 at 4081.851 kb on - strand, within Pf6N2E2_3501 at 4081.851 kb on - strand, within Pf6N2E2_3501 at 4081.851 kb on - strand, within Pf6N2E2_3501 at 4081.862 kb on + strand, within Pf6N2E2_3501 at 4081.862 kb on + strand, within Pf6N2E2_3501 at 4081.863 kb on - strand, within Pf6N2E2_3501 at 4081.871 kb on + strand, within Pf6N2E2_3501 at 4081.871 kb on + strand, within Pf6N2E2_3501 at 4081.871 kb on + strand, within Pf6N2E2_3501 at 4081.872 kb on - strand, within Pf6N2E2_3501 at 4081.872 kb on - strand, within Pf6N2E2_3501 at 4081.872 kb on - strand, within Pf6N2E2_3501 at 4081.872 kb on - strand, within Pf6N2E2_3501 at 4081.872 kb on - strand, within Pf6N2E2_3501 at 4081.874 kb on + strand, within Pf6N2E2_3501 at 4081.874 kb on + strand, within Pf6N2E2_3501 at 4081.874 kb on + strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4081.875 kb on - strand, within Pf6N2E2_3501 at 4082.155 kb on + strand at 4082.156 kb on - strand at 4082.403 kb on + strand, within Pf6N2E2_3502 at 4082.403 kb on + strand, within Pf6N2E2_3502 at 4082.403 kb on + strand, within Pf6N2E2_3502 at 4082.404 kb on - strand, within Pf6N2E2_3502 at 4082.404 kb on - strand, within Pf6N2E2_3502 at 4082.463 kb on - strand, within Pf6N2E2_3502 at 4082.573 kb on - strand, within Pf6N2E2_3502 at 4082.573 kb on - strand, within Pf6N2E2_3502 at 4082.573 kb on - strand, within Pf6N2E2_3502 at 4082.735 kb on - strand, within Pf6N2E2_3502 at 4082.936 kb on - strand, within Pf6N2E2_3502 at 4082.973 kb on - strand, within Pf6N2E2_3502 at 4083.042 kb on + strand, within Pf6N2E2_3502
Per-strain Table
Position Strand Gene LocusTag Fraction PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x remove 4,080,512 - Pf6N2E2_3500 0.34 +2.0 4,080,512 - Pf6N2E2_3500 0.34 +1.6 4,080,521 + Pf6N2E2_3500 0.34 -0.3 4,080,521 + Pf6N2E2_3500 0.34 +0.2 4,081,058 + Pf6N2E2_3500 0.74 -0.1 4,081,058 + Pf6N2E2_3500 0.74 +0.0 4,081,058 + Pf6N2E2_3500 0.74 -1.6 4,081,058 + Pf6N2E2_3500 0.74 -0.2 4,081,059 - Pf6N2E2_3500 0.74 -0.0 4,081,059 - Pf6N2E2_3500 0.74 -0.0 4,081,126 + Pf6N2E2_3500 0.79 -0.6 4,081,132 + Pf6N2E2_3500 0.80 +0.5 4,081,132 + Pf6N2E2_3500 0.80 +1.8 4,081,132 + Pf6N2E2_3500 0.80 +0.3 4,081,133 - Pf6N2E2_3500 0.80 -0.2 4,081,133 - Pf6N2E2_3500 0.80 -0.0 4,081,133 - Pf6N2E2_3500 0.80 +0.1 4,081,133 - Pf6N2E2_3500 0.80 -0.1 4,081,141 + Pf6N2E2_3500 0.80 +0.5 4,081,177 + Pf6N2E2_3500 0.83 +0.3 4,081,177 + Pf6N2E2_3500 0.83 +0.3 4,081,177 + Pf6N2E2_3500 0.83 -0.7 4,081,177 + Pf6N2E2_3500 0.83 -0.3 4,081,178 - Pf6N2E2_3500 0.83 -0.1 4,081,178 - Pf6N2E2_3500 0.83 +0.3 4,081,178 - Pf6N2E2_3500 0.83 -1.4 4,081,302 - -0.4 4,081,302 - -0.2 4,081,405 + -0.9 4,081,406 - +0.1 4,081,552 - Pf6N2E2_3501 0.18 -1.0 4,081,598 + Pf6N2E2_3501 0.23 -0.1 4,081,598 + Pf6N2E2_3501 0.23 +0.8 4,081,598 + Pf6N2E2_3501 0.23 +0.5 4,081,599 - Pf6N2E2_3501 0.24 +0.9 4,081,599 - Pf6N2E2_3501 0.24 +0.1 4,081,599 - Pf6N2E2_3501 0.24 +0.6 4,081,602 + Pf6N2E2_3501 0.24 +0.1 4,081,602 + Pf6N2E2_3501 0.24 +0.4 4,081,602 + Pf6N2E2_3501 0.24 -0.1 4,081,602 + Pf6N2E2_3501 0.24 +0.0 4,081,602 + Pf6N2E2_3501 0.24 +0.0 4,081,602 + Pf6N2E2_3501 0.24 -0.3 4,081,603 - Pf6N2E2_3501 0.24 -1.6 4,081,603 - Pf6N2E2_3501 0.24 -0.3 4,081,850 + Pf6N2E2_3501 0.55 +2.3 4,081,851 - Pf6N2E2_3501 0.55 +0.7 4,081,851 - Pf6N2E2_3501 0.55 -0.1 4,081,851 - Pf6N2E2_3501 0.55 -0.2 4,081,862 + Pf6N2E2_3501 0.57 +0.5 4,081,862 + Pf6N2E2_3501 0.57 -1.2 4,081,863 - Pf6N2E2_3501 0.57 +0.4 4,081,871 + Pf6N2E2_3501 0.58 +0.6 4,081,871 + Pf6N2E2_3501 0.58 -0.2 4,081,871 + Pf6N2E2_3501 0.58 +0.3 4,081,872 - Pf6N2E2_3501 0.58 -0.4 4,081,872 - Pf6N2E2_3501 0.58 +0.4 4,081,872 - Pf6N2E2_3501 0.58 -1.0 4,081,872 - Pf6N2E2_3501 0.58 +1.1 4,081,872 - Pf6N2E2_3501 0.58 +0.5 4,081,874 + Pf6N2E2_3501 0.58 +0.0 4,081,874 + Pf6N2E2_3501 0.58 +1.1 4,081,874 + Pf6N2E2_3501 0.58 +1.3 4,081,875 - Pf6N2E2_3501 0.58 -0.4 4,081,875 - Pf6N2E2_3501 0.58 +0.6 4,081,875 - Pf6N2E2_3501 0.58 +0.4 4,081,875 - Pf6N2E2_3501 0.58 -0.4 4,081,875 - Pf6N2E2_3501 0.58 -0.4 4,081,875 - Pf6N2E2_3501 0.58 +0.1 4,081,875 - Pf6N2E2_3501 0.58 -0.2 4,081,875 - Pf6N2E2_3501 0.58 +0.3 4,082,155 + +0.9 4,082,156 - -0.9 4,082,403 + Pf6N2E2_3502 0.19 +0.2 4,082,403 + Pf6N2E2_3502 0.19 -0.4 4,082,403 + Pf6N2E2_3502 0.19 -0.2 4,082,404 - Pf6N2E2_3502 0.19 +0.9 4,082,404 - Pf6N2E2_3502 0.19 +1.5 4,082,463 - Pf6N2E2_3502 0.25 +0.9 4,082,573 - Pf6N2E2_3502 0.35 -0.2 4,082,573 - Pf6N2E2_3502 0.35 -0.4 4,082,573 - Pf6N2E2_3502 0.35 -0.7 4,082,735 - Pf6N2E2_3502 0.51 +0.1 4,082,936 - Pf6N2E2_3502 0.70 -0.5 4,082,973 - Pf6N2E2_3502 0.73 -0.6 4,083,042 + Pf6N2E2_3502 0.80 -0.4
Or see this region's nucleotide sequence