Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3263

Experiment: PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3262 and Pf6N2E2_3263 are separated by 0 nucleotidesPf6N2E2_3263 and Pf6N2E2_3264 are separated by 7 nucleotidesPf6N2E2_3264 and Pf6N2E2_3265 overlap by 14 nucleotides Pf6N2E2_3262 - Arabinose 5-phosphate isomerase (EC 5.3.1.13), at 3,769,774 to 3,770,748 _3262 Pf6N2E2_3263 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (EC 3.1.3.45), at 3,770,749 to 3,771,273 _3263 Pf6N2E2_3264 - Uncharacterized protein YrbK clustered with lipopolysaccharide transporters, at 3,771,281 to 3,771,850 _3264 Pf6N2E2_3265 - LptA, protein essential for LPS transport across the periplasm, at 3,771,837 to 3,772,388 _3265 Position (kb) 3770 3771 3772Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3769.761 kb on + strandat 3770.746 kb on + strandat 3771.716 kb on + strand, within Pf6N2E2_3264at 3771.716 kb on + strand, within Pf6N2E2_3264at 3771.716 kb on + strand, within Pf6N2E2_3264at 3771.716 kb on + strand, within Pf6N2E2_3264at 3771.825 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x
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3,769,761 + +0.1
3,770,746 + -1.2
3,771,716 + Pf6N2E2_3264 0.76 -5.1
3,771,716 + Pf6N2E2_3264 0.76 -4.3
3,771,716 + Pf6N2E2_3264 0.76 -4.5
3,771,716 + Pf6N2E2_3264 0.76 -4.1
3,771,825 + -0.4

Or see this region's nucleotide sequence