Experiment: PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3260 and Pf6N2E2_3261 are separated by 9 nucleotides Pf6N2E2_3261 and Pf6N2E2_3262 are separated by 136 nucleotides Pf6N2E2_3262 and Pf6N2E2_3263 are separated by 0 nucleotides Pf6N2E2_3263 and Pf6N2E2_3264 are separated by 7 nucleotides
Pf6N2E2_3260 - Uncharacterized ABC transporter, ATP-binding protein YrbF, at 3,768,690 to 3,769,499
_3260
Pf6N2E2_3261 - hypothetical protein, at 3,769,509 to 3,769,637
_3261
Pf6N2E2_3262 - Arabinose 5-phosphate isomerase (EC 5.3.1.13), at 3,769,774 to 3,770,748
_3262
Pf6N2E2_3263 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (EC 3.1.3.45), at 3,770,749 to 3,771,273
_3263
Pf6N2E2_3264 - Uncharacterized protein YrbK clustered with lipopolysaccharide transporters, at 3,771,281 to 3,771,850
_3264
Position (kb) 3769
3770
3771 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.853 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3768.857 kb on + strand, within Pf6N2E2_3260 at 3769.481 kb on - strand at 3769.501 kb on + strand at 3769.502 kb on - strand at 3769.502 kb on - strand at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.590 kb on - strand, within Pf6N2E2_3261 at 3769.591 kb on + strand, within Pf6N2E2_3261 at 3769.591 kb on + strand, within Pf6N2E2_3261 at 3769.592 kb on - strand, within Pf6N2E2_3261 at 3769.592 kb on - strand, within Pf6N2E2_3261 at 3769.592 kb on - strand, within Pf6N2E2_3261 at 3769.646 kb on + strand at 3769.666 kb on + strand at 3769.708 kb on + strand at 3769.708 kb on + strand at 3769.708 kb on + strand at 3769.708 kb on + strand at 3769.736 kb on + strand at 3769.761 kb on + strand at 3770.746 kb on + strand at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.716 kb on + strand, within Pf6N2E2_3264
Per-strain Table
Position Strand Gene LocusTag Fraction PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x remove 3,768,853 + Pf6N2E2_3260 0.20 -0.8 3,768,853 + Pf6N2E2_3260 0.20 -3.5 3,768,853 + Pf6N2E2_3260 0.20 -3.2 3,768,853 + Pf6N2E2_3260 0.20 -0.5 3,768,853 + Pf6N2E2_3260 0.20 -4.2 3,768,853 + Pf6N2E2_3260 0.20 -2.0 3,768,853 + Pf6N2E2_3260 0.20 -2.6 3,768,853 + Pf6N2E2_3260 0.20 -2.9 3,768,853 + Pf6N2E2_3260 0.20 -1.6 3,768,853 + Pf6N2E2_3260 0.20 +1.8 3,768,857 + Pf6N2E2_3260 0.21 -1.7 3,768,857 + Pf6N2E2_3260 0.21 -1.9 3,768,857 + Pf6N2E2_3260 0.21 -4.2 3,768,857 + Pf6N2E2_3260 0.21 -2.5 3,768,857 + Pf6N2E2_3260 0.21 -2.6 3,768,857 + Pf6N2E2_3260 0.21 -2.6 3,768,857 + Pf6N2E2_3260 0.21 -3.8 3,768,857 + Pf6N2E2_3260 0.21 -2.6 3,768,857 + Pf6N2E2_3260 0.21 -2.8 3,768,857 + Pf6N2E2_3260 0.21 -1.2 3,769,481 - -2.1 3,769,501 + -3.3 3,769,502 - -1.8 3,769,502 - -2.0 3,769,590 - Pf6N2E2_3261 0.63 -1.3 3,769,590 - Pf6N2E2_3261 0.63 -0.8 3,769,590 - Pf6N2E2_3261 0.63 -1.9 3,769,590 - Pf6N2E2_3261 0.63 +1.8 3,769,590 - Pf6N2E2_3261 0.63 -0.5 3,769,590 - Pf6N2E2_3261 0.63 -2.4 3,769,590 - Pf6N2E2_3261 0.63 -0.6 3,769,591 + Pf6N2E2_3261 0.64 -2.0 3,769,591 + Pf6N2E2_3261 0.64 -3.0 3,769,592 - Pf6N2E2_3261 0.64 +0.4 3,769,592 - Pf6N2E2_3261 0.64 -0.8 3,769,592 - Pf6N2E2_3261 0.64 -0.0 3,769,646 + +1.8 3,769,666 + -1.0 3,769,708 + -0.7 3,769,708 + +0.8 3,769,708 + -0.2 3,769,708 + -1.0 3,769,736 + +0.1 3,769,761 + +0.1 3,770,746 + -1.2 3,771,716 + Pf6N2E2_3264 0.76 -5.1 3,771,716 + Pf6N2E2_3264 0.76 -4.3 3,771,716 + Pf6N2E2_3264 0.76 -4.5 3,771,716 + Pf6N2E2_3264 0.76 -4.1
Or see this region's nucleotide sequence