Experiment: PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3025 and Pf6N2E2_3026 overlap by 4 nucleotides Pf6N2E2_3026 and Pf6N2E2_3027 are separated by 11 nucleotides Pf6N2E2_3027 and Pf6N2E2_3028 are separated by 3 nucleotides Pf6N2E2_3028 and Pf6N2E2_3029 are separated by 36 nucleotides
Pf6N2E2_3025 - Baseplate assembly protein J, at 3,511,244 to 3,512,239
_3025
Pf6N2E2_3026 - Baseplate assembly protein W, at 3,512,236 to 3,512,568
_3026
Pf6N2E2_3027 - Baseplate assembly protein V, at 3,512,580 to 3,513,191
_3027
Pf6N2E2_3028 - FIG00959937: hypothetical protein, at 3,513,195 to 3,513,707
_3028
Pf6N2E2_3029 - Holin, at 3,513,744 to 3,514,094
_3029
Position (kb)
3512
3513
3514 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3511.716 kb on + strand, within Pf6N2E2_3025 at 3511.716 kb on + strand, within Pf6N2E2_3025 at 3511.716 kb on + strand, within Pf6N2E2_3025 at 3511.717 kb on - strand, within Pf6N2E2_3025 at 3511.717 kb on - strand, within Pf6N2E2_3025 at 3511.730 kb on + strand, within Pf6N2E2_3025 at 3511.730 kb on + strand, within Pf6N2E2_3025 at 3511.730 kb on + strand, within Pf6N2E2_3025 at 3511.731 kb on - strand, within Pf6N2E2_3025 at 3511.780 kb on + strand, within Pf6N2E2_3025 at 3511.840 kb on - strand, within Pf6N2E2_3025 at 3511.840 kb on - strand, within Pf6N2E2_3025 at 3511.873 kb on - strand, within Pf6N2E2_3025 at 3512.010 kb on + strand, within Pf6N2E2_3025 at 3512.010 kb on + strand, within Pf6N2E2_3025 at 3512.011 kb on - strand, within Pf6N2E2_3025 at 3512.011 kb on - strand, within Pf6N2E2_3025 at 3512.011 kb on - strand, within Pf6N2E2_3025 at 3512.133 kb on + strand, within Pf6N2E2_3025 at 3512.133 kb on + strand, within Pf6N2E2_3025 at 3512.133 kb on + strand, within Pf6N2E2_3025 at 3512.134 kb on - strand, within Pf6N2E2_3025 at 3512.366 kb on + strand, within Pf6N2E2_3026 at 3512.367 kb on - strand, within Pf6N2E2_3026 at 3512.453 kb on + strand, within Pf6N2E2_3026 at 3512.453 kb on + strand, within Pf6N2E2_3026 at 3512.454 kb on - strand, within Pf6N2E2_3026 at 3512.454 kb on - strand, within Pf6N2E2_3026 at 3512.454 kb on - strand, within Pf6N2E2_3026 at 3512.454 kb on - strand, within Pf6N2E2_3026 at 3512.454 kb on - strand, within Pf6N2E2_3026 at 3512.512 kb on + strand, within Pf6N2E2_3026 at 3512.512 kb on + strand, within Pf6N2E2_3026 at 3512.548 kb on - strand at 3512.582 kb on - strand at 3512.721 kb on - strand, within Pf6N2E2_3027 at 3513.214 kb on + strand at 3513.214 kb on + strand at 3513.214 kb on + strand at 3513.240 kb on + strand at 3513.252 kb on - strand, within Pf6N2E2_3028 at 3513.305 kb on - strand, within Pf6N2E2_3028 at 3513.340 kb on + strand, within Pf6N2E2_3028 at 3513.341 kb on - strand, within Pf6N2E2_3028 at 3513.341 kb on - strand, within Pf6N2E2_3028 at 3513.341 kb on - strand, within Pf6N2E2_3028 at 3513.341 kb on - strand, within Pf6N2E2_3028 at 3513.640 kb on + strand, within Pf6N2E2_3028 at 3513.641 kb on - strand, within Pf6N2E2_3028 at 3513.724 kb on + strand at 3513.724 kb on + strand at 3513.725 kb on - strand at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.901 kb on + strand, within Pf6N2E2_3029 at 3513.902 kb on - strand, within Pf6N2E2_3029 at 3513.902 kb on - strand, within Pf6N2E2_3029 at 3514.116 kb on + strand at 3514.117 kb on - strand at 3514.136 kb on - strand at 3514.163 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x remove 3,511,716 + Pf6N2E2_3025 0.47 -0.0 3,511,716 + Pf6N2E2_3025 0.47 +0.5 3,511,716 + Pf6N2E2_3025 0.47 -0.8 3,511,717 - Pf6N2E2_3025 0.47 +0.7 3,511,717 - Pf6N2E2_3025 0.47 +0.0 3,511,730 + Pf6N2E2_3025 0.49 -0.1 3,511,730 + Pf6N2E2_3025 0.49 +0.4 3,511,730 + Pf6N2E2_3025 0.49 +0.1 3,511,731 - Pf6N2E2_3025 0.49 +0.7 3,511,780 + Pf6N2E2_3025 0.54 +0.6 3,511,840 - Pf6N2E2_3025 0.60 -1.5 3,511,840 - Pf6N2E2_3025 0.60 +0.1 3,511,873 - Pf6N2E2_3025 0.63 +0.1 3,512,010 + Pf6N2E2_3025 0.77 -1.2 3,512,010 + Pf6N2E2_3025 0.77 +0.8 3,512,011 - Pf6N2E2_3025 0.77 +0.2 3,512,011 - Pf6N2E2_3025 0.77 -0.4 3,512,011 - Pf6N2E2_3025 0.77 -0.0 3,512,133 + Pf6N2E2_3025 0.89 -1.3 3,512,133 + Pf6N2E2_3025 0.89 +0.1 3,512,133 + Pf6N2E2_3025 0.89 +0.3 3,512,134 - Pf6N2E2_3025 0.89 -0.1 3,512,366 + Pf6N2E2_3026 0.39 +0.5 3,512,367 - Pf6N2E2_3026 0.39 -1.1 3,512,453 + Pf6N2E2_3026 0.65 +0.1 3,512,453 + Pf6N2E2_3026 0.65 -0.1 3,512,454 - Pf6N2E2_3026 0.65 -0.3 3,512,454 - Pf6N2E2_3026 0.65 +0.3 3,512,454 - Pf6N2E2_3026 0.65 +0.6 3,512,454 - Pf6N2E2_3026 0.65 +0.3 3,512,454 - Pf6N2E2_3026 0.65 +1.1 3,512,512 + Pf6N2E2_3026 0.83 +0.4 3,512,512 + Pf6N2E2_3026 0.83 -0.6 3,512,548 - -0.6 3,512,582 - -0.2 3,512,721 - Pf6N2E2_3027 0.23 +0.2 3,513,214 + -2.3 3,513,214 + -0.6 3,513,214 + +0.6 3,513,240 + -0.6 3,513,252 - Pf6N2E2_3028 0.11 +1.8 3,513,305 - Pf6N2E2_3028 0.21 -1.0 3,513,340 + Pf6N2E2_3028 0.28 +0.2 3,513,341 - Pf6N2E2_3028 0.28 +0.2 3,513,341 - Pf6N2E2_3028 0.28 -0.3 3,513,341 - Pf6N2E2_3028 0.28 -0.3 3,513,341 - Pf6N2E2_3028 0.28 +0.2 3,513,640 + Pf6N2E2_3028 0.87 -0.2 3,513,641 - Pf6N2E2_3028 0.87 -1.5 3,513,724 + -0.2 3,513,724 + +0.8 3,513,725 - +1.8 3,513,901 + Pf6N2E2_3029 0.45 +0.0 3,513,901 + Pf6N2E2_3029 0.45 +0.8 3,513,901 + Pf6N2E2_3029 0.45 -0.5 3,513,901 + Pf6N2E2_3029 0.45 -0.0 3,513,901 + Pf6N2E2_3029 0.45 -0.8 3,513,901 + Pf6N2E2_3029 0.45 +0.9 3,513,901 + Pf6N2E2_3029 0.45 -0.2 3,513,902 - Pf6N2E2_3029 0.45 +1.8 3,513,902 - Pf6N2E2_3029 0.45 +0.5 3,514,116 + +1.8 3,514,117 - +0.4 3,514,136 - +2.4 3,514,163 - +0.1
Or see this region's nucleotide sequence