Experiment: PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1481 and Pf6N2E2_1482 are separated by 152 nucleotides Pf6N2E2_1482 and Pf6N2E2_1483 are separated by 192 nucleotides Pf6N2E2_1483 and Pf6N2E2_1484 are separated by 28 nucleotides
Pf6N2E2_1481 - VgrG protein, at 1,730,306 to 1,731,634
_1481
Pf6N2E2_1482 - Probable periplasmic spermidine/putrescine-binding protein, at 1,731,787 to 1,732,890
_1482
Pf6N2E2_1483 - transcriptional regulator, LuxR family, at 1,733,083 to 1,733,820
_1483
Pf6N2E2_1484 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 1,733,849 to 1,734,778
_1484
Position (kb)
1731
1732
1733 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1730.858 kb on - strand, within Pf6N2E2_1481 at 1730.871 kb on - strand, within Pf6N2E2_1481 at 1730.897 kb on - strand, within Pf6N2E2_1481 at 1730.899 kb on - strand, within Pf6N2E2_1481 at 1730.949 kb on + strand, within Pf6N2E2_1481 at 1730.950 kb on - strand, within Pf6N2E2_1481 at 1730.950 kb on - strand, within Pf6N2E2_1481 at 1731.189 kb on + strand, within Pf6N2E2_1481 at 1731.201 kb on + strand, within Pf6N2E2_1481 at 1731.216 kb on + strand, within Pf6N2E2_1481 at 1731.310 kb on - strand, within Pf6N2E2_1481 at 1731.310 kb on - strand, within Pf6N2E2_1481 at 1731.340 kb on - strand, within Pf6N2E2_1481 at 1731.411 kb on + strand at 1731.528 kb on + strand at 1731.529 kb on - strand at 1731.650 kb on + strand at 1731.654 kb on + strand at 1731.655 kb on - strand at 1731.740 kb on - strand at 1731.772 kb on - strand at 1731.864 kb on + strand at 1731.865 kb on - strand at 1732.007 kb on + strand, within Pf6N2E2_1482 at 1732.188 kb on + strand, within Pf6N2E2_1482 at 1732.189 kb on - strand, within Pf6N2E2_1482 at 1732.241 kb on + strand, within Pf6N2E2_1482 at 1732.242 kb on - strand, within Pf6N2E2_1482 at 1732.280 kb on + strand, within Pf6N2E2_1482 at 1732.296 kb on + strand, within Pf6N2E2_1482 at 1732.297 kb on - strand, within Pf6N2E2_1482 at 1732.472 kb on + strand, within Pf6N2E2_1482 at 1732.495 kb on + strand, within Pf6N2E2_1482 at 1732.496 kb on - strand, within Pf6N2E2_1482 at 1732.500 kb on - strand, within Pf6N2E2_1482 at 1732.588 kb on - strand, within Pf6N2E2_1482 at 1732.791 kb on + strand at 1732.792 kb on - strand at 1732.792 kb on - strand at 1732.792 kb on - strand at 1732.793 kb on + strand at 1732.793 kb on + strand at 1732.800 kb on - strand at 1732.800 kb on - strand at 1732.800 kb on - strand at 1732.800 kb on - strand at 1732.834 kb on + strand at 1732.948 kb on + strand at 1732.951 kb on - strand at 1732.968 kb on - strand at 1732.970 kb on + strand at 1732.971 kb on - strand at 1732.984 kb on + strand at 1732.984 kb on + strand at 1732.984 kb on + strand at 1732.985 kb on - strand at 1732.989 kb on - strand at 1733.015 kb on + strand at 1733.487 kb on - strand, within Pf6N2E2_1483 at 1733.700 kb on - strand, within Pf6N2E2_1483
Per-strain Table
Position Strand Gene LocusTag Fraction PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x remove 1,730,858 - Pf6N2E2_1481 0.42 +0.7 1,730,871 - Pf6N2E2_1481 0.43 +0.2 1,730,897 - Pf6N2E2_1481 0.44 +0.7 1,730,899 - Pf6N2E2_1481 0.45 -0.3 1,730,949 + Pf6N2E2_1481 0.48 +0.5 1,730,950 - Pf6N2E2_1481 0.48 +0.7 1,730,950 - Pf6N2E2_1481 0.48 +0.2 1,731,189 + Pf6N2E2_1481 0.66 -1.3 1,731,201 + Pf6N2E2_1481 0.67 +0.5 1,731,216 + Pf6N2E2_1481 0.68 +0.2 1,731,310 - Pf6N2E2_1481 0.76 -0.4 1,731,310 - Pf6N2E2_1481 0.76 +1.0 1,731,340 - Pf6N2E2_1481 0.78 -1.2 1,731,411 + +0.4 1,731,528 + -0.8 1,731,529 - +0.0 1,731,650 + +1.0 1,731,654 + +0.4 1,731,655 - -1.2 1,731,740 - -0.6 1,731,772 - -0.2 1,731,864 + -0.4 1,731,865 - +0.8 1,732,007 + Pf6N2E2_1482 0.20 -1.6 1,732,188 + Pf6N2E2_1482 0.36 -0.7 1,732,189 - Pf6N2E2_1482 0.36 -0.2 1,732,241 + Pf6N2E2_1482 0.41 -1.1 1,732,242 - Pf6N2E2_1482 0.41 -0.7 1,732,280 + Pf6N2E2_1482 0.45 +0.7 1,732,296 + Pf6N2E2_1482 0.46 -0.5 1,732,297 - Pf6N2E2_1482 0.46 +0.6 1,732,472 + Pf6N2E2_1482 0.62 -3.0 1,732,495 + Pf6N2E2_1482 0.64 -0.1 1,732,496 - Pf6N2E2_1482 0.64 -0.8 1,732,500 - Pf6N2E2_1482 0.65 +0.5 1,732,588 - Pf6N2E2_1482 0.73 -1.2 1,732,791 + -0.3 1,732,792 - +2.2 1,732,792 - -0.4 1,732,792 - -0.9 1,732,793 + +0.7 1,732,793 + +2.2 1,732,800 - +0.7 1,732,800 - +1.1 1,732,800 - +0.8 1,732,800 - -1.8 1,732,834 + -1.1 1,732,948 + +1.9 1,732,951 - +0.5 1,732,968 - +0.2 1,732,970 + -0.4 1,732,971 - -0.3 1,732,984 + +1.2 1,732,984 + +2.2 1,732,984 + -0.5 1,732,985 - -0.0 1,732,989 - -0.3 1,733,015 + -1.8 1,733,487 - Pf6N2E2_1483 0.55 -0.1 1,733,700 - Pf6N2E2_1483 0.84 +1.2
Or see this region's nucleotide sequence