Experiment: Post-colonization (Day 2) - Cage5;Mouse1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2007 and BT_2008 are separated by 89 nucleotides BT_2008 and BT2009 are separated by 28 nucleotides BT2009 and BT2010 overlap by 4 nucleotides BT2010 and BT2011 are separated by 95 nucleotides BT2011 and BT2012 overlap by 38 nucleotides
BT2007: BT2007 - conserved hypothetical protein, putative glycoprotease (NCBI ptt file), at 2,523,434 to 2,524,129
BT2007
BT_2008: BT_2008 - hypothetical protein (RefSeq), at 2,524,219 to 2,525,097
_2008
BT2009: BT2009 - guanylate kinase (GMP kinase) (NCBI ptt file), at 2,525,126 to 2,525,740
BT2009
BT2010: BT2010 - hypothetical protein (NCBI ptt file), at 2,525,737 to 2,525,991
BT2010
BT2011: BT2011 - putative nicotinate-nucleotide adenylyltransferase (NCBI ptt file), at 2,526,087 to 2,526,695
BT2011
BT2012: BT2012 - conserved hypothetical protein, putative ATPase (NCBI ptt file), at 2,526,658 to 2,527,947
BT2012
Position (kb)
2525
2526 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2524.145 kb on - strand at 2524.283 kb on + strand at 2524.713 kb on + strand, within BT_2008 at 2524.724 kb on + strand, within BT_2008 at 2524.819 kb on + strand, within BT_2008 at 2525.831 kb on + strand, within BT2010 at 2525.835 kb on + strand, within BT2010 at 2526.041 kb on + strand at 2526.659 kb on + strand at 2526.659 kb on + strand at 2526.660 kb on - strand at 2526.668 kb on + strand at 2526.669 kb on - strand at 2526.693 kb on + strand at 2526.693 kb on + strand at 2526.694 kb on - strand at 2526.736 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Post-colonization (Day 2) - Cage5;Mouse1 remove 2,524,145 - -0.5 2,524,283 + -1.1 2,524,713 + BT_2008 0.56 +0.0 2,524,724 + BT_2008 0.57 -3.4 2,524,819 + BT_2008 0.68 -2.1 2,525,831 + BT2010 0.37 +0.7 2,525,835 + BT2010 0.38 -0.4 2,526,041 + -2.1 2,526,659 + -0.4 2,526,659 + -2.7 2,526,660 - -2.3 2,526,668 + +1.8 2,526,669 - -1.2 2,526,693 + -0.9 2,526,693 + -1.8 2,526,694 - -0.5 2,526,736 + -1.3
Or see this region's nucleotide sequence