Experiment: NaNO3 10 mM as N source, buffered with TES 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11020 and SGL_RS11025 are separated by 381 nucleotides SGL_RS11025 and SGL_RS11030 are separated by 16 nucleotides SGL_RS11030 and SGL_RS11035 are separated by 76 nucleotides
SGL_RS11020: SGL_RS11020 - geranylgeranyl reductase, at 1,934,307 to 1,935,530
_RS11020
SGL_RS11025: SGL_RS11025 - oxidoreductase, at 1,935,912 to 1,937,069
_RS11025
SGL_RS11030: SGL_RS11030 - DUF433 domain-containing protein, at 1,937,086 to 1,937,397
_RS11030
SGL_RS11035: SGL_RS11035 - LapA family protein, at 1,937,474 to 1,938,310
_RS11035
Position (kb)
1935
1936
1937
1938 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1935.607 kb on + strand at 1935.698 kb on - strand at 1935.698 kb on - strand at 1935.832 kb on + strand at 1935.895 kb on - strand at 1936.031 kb on + strand, within SGL_RS11025 at 1936.124 kb on - strand, within SGL_RS11025 at 1936.232 kb on - strand, within SGL_RS11025 at 1936.362 kb on + strand, within SGL_RS11025 at 1936.362 kb on + strand, within SGL_RS11025 at 1936.370 kb on - strand, within SGL_RS11025 at 1936.370 kb on - strand, within SGL_RS11025 at 1936.370 kb on - strand, within SGL_RS11025 at 1936.621 kb on - strand, within SGL_RS11025 at 1936.655 kb on - strand, within SGL_RS11025 at 1936.725 kb on - strand, within SGL_RS11025 at 1936.815 kb on + strand, within SGL_RS11025 at 1937.474 kb on + strand at 1937.641 kb on + strand, within SGL_RS11035 at 1937.902 kb on - strand, within SGL_RS11035 at 1937.978 kb on + strand, within SGL_RS11035
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO3 10 mM as N source, buffered with TES 20 mM TES pH8 remove 1,935,607 + -0.8 1,935,698 - +0.2 1,935,698 - +0.3 1,935,832 + -0.0 1,935,895 - +0.4 1,936,031 + SGL_RS11025 0.10 -3.7 1,936,124 - SGL_RS11025 0.18 -2.1 1,936,232 - SGL_RS11025 0.28 -1.5 1,936,362 + SGL_RS11025 0.39 +0.5 1,936,362 + SGL_RS11025 0.39 -2.1 1,936,370 - SGL_RS11025 0.40 -2.4 1,936,370 - SGL_RS11025 0.40 -1.9 1,936,370 - SGL_RS11025 0.40 -0.1 1,936,621 - SGL_RS11025 0.61 -0.8 1,936,655 - SGL_RS11025 0.64 -1.1 1,936,725 - SGL_RS11025 0.70 -0.4 1,936,815 + SGL_RS11025 0.78 -1.7 1,937,474 + -0.4 1,937,641 + SGL_RS11035 0.20 -3.5 1,937,902 - SGL_RS11035 0.51 -1.5 1,937,978 + SGL_RS11035 0.60 +0.7
Or see this region's nucleotide sequence