Experiment: NaNO3 10 mM as N source, buffered with TES 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04650 and SGL_RS04655 are separated by 82 nucleotides SGL_RS04655 and SGL_RS04660 are separated by 186 nucleotides SGL_RS04660 and SGL_RS04665 are separated by 343 nucleotides
SGL_RS04650: SGL_RS04650 - tetratricopeptide repeat protein, at 594,454 to 595,650
_RS04650
SGL_RS04655: SGL_RS04655 - phycobiliprotein lyase, at 595,733 to 596,311
_RS04655
SGL_RS04660: SGL_RS04660 - phycobilisome rod-core linker polypeptide, at 596,498 to 597,247
_RS04660
SGL_RS04665: SGL_RS04665 - hypothetical protein, at 597,591 to 598,487
_RS04665
Position (kb) 596
597
598 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 595.524 kb on + strand, within SGL_RS04650 at 595.524 kb on + strand, within SGL_RS04650 at 595.594 kb on + strand at 595.664 kb on + strand at 596.489 kb on + strand at 596.509 kb on - strand at 596.570 kb on + strand at 596.755 kb on + strand, within SGL_RS04660 at 597.002 kb on - strand, within SGL_RS04660 at 597.168 kb on + strand, within SGL_RS04660 at 597.263 kb on - strand at 597.358 kb on - strand at 597.558 kb on - strand at 597.587 kb on + strand at 597.637 kb on + strand at 597.637 kb on + strand at 597.939 kb on - strand, within SGL_RS04665 at 598.040 kb on + strand, within SGL_RS04665 at 598.087 kb on + strand, within SGL_RS04665 at 598.087 kb on + strand, within SGL_RS04665
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO3 10 mM as N source, buffered with TES 20 mM TES pH8 remove 595,524 + SGL_RS04650 0.89 +0.0 595,524 + SGL_RS04650 0.89 +0.2 595,594 + -0.1 595,664 + +0.0 596,489 + -1.1 596,509 - -2.8 596,570 + -1.1 596,755 + SGL_RS04660 0.34 -2.2 597,002 - SGL_RS04660 0.67 -0.8 597,168 + SGL_RS04660 0.89 -1.7 597,263 - -1.7 597,358 - -0.0 597,558 - -0.3 597,587 + +0.0 597,637 + +0.1 597,637 + -0.2 597,939 - SGL_RS04665 0.39 -0.7 598,040 + SGL_RS04665 0.50 -0.2 598,087 + SGL_RS04665 0.55 -0.0 598,087 + SGL_RS04665 0.55 -0.0
Or see this region's nucleotide sequence