Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS18350 and LRK55_RS18355 are separated by 152 nucleotides LRK55_RS18355 and LRK55_RS18360 are separated by 44 nucleotides LRK55_RS18360 and LRK55_RS18365 overlap by 11 nucleotides
LRK55_RS18350: LRK55_RS18350 - thermonuclease family protein, at 2,917 to 3,636
_RS18350
LRK55_RS18355: LRK55_RS18355 - hypothetical protein, at 3,789 to 4,352
_RS18355
LRK55_RS18360: LRK55_RS18360 - hypothetical protein, at 4,397 to 5,383
_RS18360
LRK55_RS18365: LRK55_RS18365 - HAMP domain-containing sensor histidine kinase, at 5,373 to 6,617
_RS18365
Position (kb) 4
5
6 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 3.399 kb on - strand, within LRK55_RS18350 at 3.443 kb on + strand, within LRK55_RS18350 at 3.444 kb on - strand, within LRK55_RS18350 at 3.444 kb on - strand, within LRK55_RS18350 at 3.544 kb on + strand, within LRK55_RS18350 at 3.544 kb on + strand, within LRK55_RS18350 at 3.544 kb on + strand, within LRK55_RS18350 at 3.545 kb on - strand, within LRK55_RS18350 at 3.545 kb on - strand, within LRK55_RS18350 at 3.545 kb on - strand, within LRK55_RS18350 at 3.545 kb on - strand, within LRK55_RS18350 at 3.545 kb on - strand, within LRK55_RS18350 at 3.545 kb on - strand, within LRK55_RS18350 at 3.634 kb on + strand at 3.642 kb on + strand at 3.643 kb on - strand at 3.748 kb on - strand at 3.748 kb on - strand at 3.748 kb on - strand at 3.803 kb on + strand at 3.803 kb on + strand at 3.803 kb on + strand at 3.803 kb on + strand at 3.804 kb on - strand at 3.804 kb on - strand at 3.804 kb on - strand at 3.804 kb on - strand at 3.804 kb on - strand at 3.804 kb on - strand at 3.938 kb on - strand, within LRK55_RS18355 at 3.969 kb on + strand, within LRK55_RS18355 at 3.969 kb on + strand, within LRK55_RS18355 at 3.970 kb on - strand, within LRK55_RS18355 at 3.970 kb on - strand, within LRK55_RS18355 at 4.033 kb on - strand, within LRK55_RS18355 at 4.033 kb on - strand, within LRK55_RS18355 at 4.069 kb on + strand, within LRK55_RS18355 at 4.135 kb on + strand, within LRK55_RS18355 at 4.136 kb on - strand, within LRK55_RS18355 at 4.136 kb on - strand, within LRK55_RS18355 at 4.136 kb on - strand, within LRK55_RS18355 at 4.212 kb on - strand, within LRK55_RS18355 at 4.212 kb on - strand, within LRK55_RS18355 at 4.353 kb on + strand at 4.354 kb on - strand at 4.431 kb on + strand at 4.431 kb on + strand at 4.431 kb on + strand at 4.431 kb on + strand at 4.432 kb on - strand at 4.484 kb on + strand at 4.484 kb on + strand at 4.484 kb on + strand at 4.484 kb on + strand at 4.485 kb on - strand at 4.485 kb on - strand at 4.485 kb on - strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.488 kb on + strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.489 kb on - strand at 4.533 kb on + strand, within LRK55_RS18360 at 4.533 kb on + strand, within LRK55_RS18360 at 4.533 kb on + strand, within LRK55_RS18360 at 4.534 kb on - strand, within LRK55_RS18360 at 4.534 kb on - strand, within LRK55_RS18360 at 4.534 kb on - strand, within LRK55_RS18360 at 4.534 kb on - strand, within LRK55_RS18360 at 4.578 kb on - strand, within LRK55_RS18360 at 4.677 kb on + strand, within LRK55_RS18360 at 4.677 kb on + strand, within LRK55_RS18360 at 4.678 kb on - strand, within LRK55_RS18360 at 4.678 kb on - strand, within LRK55_RS18360 at 4.678 kb on - strand, within LRK55_RS18360 at 4.678 kb on - strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.746 kb on + strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.747 kb on - strand, within LRK55_RS18360 at 4.859 kb on - strand, within LRK55_RS18360 at 4.859 kb on - strand, within LRK55_RS18360 at 4.869 kb on + strand, within LRK55_RS18360 at 4.869 kb on + strand, within LRK55_RS18360 at 4.869 kb on + strand, within LRK55_RS18360 at 4.869 kb on + strand, within LRK55_RS18360 at 4.869 kb on + strand, within LRK55_RS18360 at 4.870 kb on - strand, within LRK55_RS18360 at 4.938 kb on + strand, within LRK55_RS18360 at 4.938 kb on + strand, within LRK55_RS18360 at 4.938 kb on + strand, within LRK55_RS18360 at 4.938 kb on + strand, within LRK55_RS18360 at 4.938 kb on + strand, within LRK55_RS18360 at 4.938 kb on + strand, within LRK55_RS18360 at 4.938 kb on + strand, within LRK55_RS18360 at 4.938 kb on + strand, within LRK55_RS18360 at 4.938 kb on + strand, within LRK55_RS18360 at 4.939 kb on - strand, within LRK55_RS18360 at 4.939 kb on - strand, within LRK55_RS18360 at 4.939 kb on - strand, within LRK55_RS18360 at 4.939 kb on - strand, within LRK55_RS18360 at 5.118 kb on + strand, within LRK55_RS18360 at 5.118 kb on + strand, within LRK55_RS18360 at 5.118 kb on + strand, within LRK55_RS18360 at 5.118 kb on + strand, within LRK55_RS18360 at 5.118 kb on + strand, within LRK55_RS18360 at 5.118 kb on + strand, within LRK55_RS18360 at 5.118 kb on + strand, within LRK55_RS18360 at 5.118 kb on + strand, within LRK55_RS18360 at 5.118 kb on + strand, within LRK55_RS18360 at 5.118 kb on + strand, within LRK55_RS18360 at 5.118 kb on + strand, within LRK55_RS18360 at 5.118 kb on + strand, within LRK55_RS18360 at 5.119 kb on - strand, within LRK55_RS18360 at 5.119 kb on - strand, within LRK55_RS18360 at 5.119 kb on - strand, within LRK55_RS18360 at 5.119 kb on - strand, within LRK55_RS18360 at 5.119 kb on - strand, within LRK55_RS18360 at 5.119 kb on - strand, within LRK55_RS18360 at 5.119 kb on - strand, within LRK55_RS18360 at 5.119 kb on - strand, within LRK55_RS18360 at 5.119 kb on - strand, within LRK55_RS18360 at 5.119 kb on - strand, within LRK55_RS18360 at 5.304 kb on + strand at 5.304 kb on + strand at 5.304 kb on + strand at 5.304 kb on + strand at 5.304 kb on + strand at 5.305 kb on - strand at 5.305 kb on - strand at 5.374 kb on + strand at 5.374 kb on + strand at 5.374 kb on + strand at 5.374 kb on + strand at 5.374 kb on + strand at 5.374 kb on + strand at 5.374 kb on + strand at 5.374 kb on + strand at 5.374 kb on + strand at 5.374 kb on + strand at 5.375 kb on - strand at 5.375 kb on - strand at 5.375 kb on - strand at 5.375 kb on - strand at 5.375 kb on - strand at 5.375 kb on - strand at 5.431 kb on + strand at 5.431 kb on + strand at 5.431 kb on + strand at 5.431 kb on + strand at 5.431 kb on + strand at 5.431 kb on + strand at 5.431 kb on + strand at 5.431 kb on + strand at 5.431 kb on + strand at 5.431 kb on + strand at 5.432 kb on - strand at 5.432 kb on - strand at 5.494 kb on + strand at 5.495 kb on - strand at 5.495 kb on - strand at 5.495 kb on - strand at 5.520 kb on - strand, within LRK55_RS18365 at 5.545 kb on + strand, within LRK55_RS18365 at 5.545 kb on + strand, within LRK55_RS18365 at 5.545 kb on + strand, within LRK55_RS18365 at 5.546 kb on - strand, within LRK55_RS18365 at 5.546 kb on - strand, within LRK55_RS18365 at 5.546 kb on - strand, within LRK55_RS18365 at 5.546 kb on - strand, within LRK55_RS18365 at 5.570 kb on + strand, within LRK55_RS18365 at 5.647 kb on + strand, within LRK55_RS18365 at 5.647 kb on + strand, within LRK55_RS18365 at 5.647 kb on + strand, within LRK55_RS18365 at 5.648 kb on - strand, within LRK55_RS18365 at 5.648 kb on - strand, within LRK55_RS18365 at 5.677 kb on + strand, within LRK55_RS18365 at 5.677 kb on + strand, within LRK55_RS18365 at 5.803 kb on + strand, within LRK55_RS18365 at 5.928 kb on + strand, within LRK55_RS18365 at 5.928 kb on + strand, within LRK55_RS18365 at 5.928 kb on + strand, within LRK55_RS18365 at 5.928 kb on + strand, within LRK55_RS18365 at 6.087 kb on + strand, within LRK55_RS18365 at 6.087 kb on + strand, within LRK55_RS18365 at 6.087 kb on + strand, within LRK55_RS18365 at 6.087 kb on + strand, within LRK55_RS18365 at 6.087 kb on + strand, within LRK55_RS18365 at 6.088 kb on - strand, within LRK55_RS18365 at 6.088 kb on - strand, within LRK55_RS18365 at 6.088 kb on - strand, within LRK55_RS18365 at 6.088 kb on - strand, within LRK55_RS18365 at 6.088 kb on - strand, within LRK55_RS18365
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 3,399 - LRK55_RS18350 0.67 +0.5 3,443 + LRK55_RS18350 0.73 +1.0 3,444 - LRK55_RS18350 0.73 +0.9 3,444 - LRK55_RS18350 0.73 +0.9 3,544 + LRK55_RS18350 0.87 +0.2 3,544 + LRK55_RS18350 0.87 -1.0 3,544 + LRK55_RS18350 0.87 -0.5 3,545 - LRK55_RS18350 0.87 -0.9 3,545 - LRK55_RS18350 0.87 +0.7 3,545 - LRK55_RS18350 0.87 +0.2 3,545 - LRK55_RS18350 0.87 +0.6 3,545 - LRK55_RS18350 0.87 -1.5 3,545 - LRK55_RS18350 0.87 -0.2 3,634 + +0.1 3,642 + +1.0 3,643 - -0.6 3,748 - -0.5 3,748 - +0.5 3,748 - +0.1 3,803 + -1.7 3,803 + +0.6 3,803 + +1.1 3,803 + +1.1 3,804 - +1.0 3,804 - +0.1 3,804 - -2.7 3,804 - +0.9 3,804 - +1.0 3,804 - +1.4 3,938 - LRK55_RS18355 0.26 -1.9 3,969 + LRK55_RS18355 0.32 +1.1 3,969 + LRK55_RS18355 0.32 +0.6 3,970 - LRK55_RS18355 0.32 +0.6 3,970 - LRK55_RS18355 0.32 +0.6 4,033 - LRK55_RS18355 0.43 +0.4 4,033 - LRK55_RS18355 0.43 -0.0 4,069 + LRK55_RS18355 0.50 +0.6 4,135 + LRK55_RS18355 0.61 -0.6 4,136 - LRK55_RS18355 0.62 +1.4 4,136 - LRK55_RS18355 0.62 +1.3 4,136 - LRK55_RS18355 0.62 -0.3 4,212 - LRK55_RS18355 0.75 -2.8 4,212 - LRK55_RS18355 0.75 +0.5 4,353 + -0.3 4,354 - +0.4 4,431 + +0.1 4,431 + +0.4 4,431 + -1.2 4,431 + -0.1 4,432 - +0.8 4,484 + -1.5 4,484 + -0.8 4,484 + -1.0 4,484 + -0.1 4,485 - -1.5 4,485 - +0.3 4,485 - +1.2 4,488 + +0.5 4,488 + -0.6 4,488 + -1.4 4,488 + +0.8 4,488 + -1.2 4,488 + +0.5 4,488 + -1.5 4,488 + -0.6 4,488 + -0.8 4,488 + +0.4 4,488 + +0.7 4,488 + -2.3 4,488 + +0.3 4,488 + +0.3 4,488 + +1.9 4,488 + +0.1 4,488 + -0.8 4,489 - -1.1 4,489 - -0.5 4,489 - +0.8 4,489 - +0.3 4,489 - +0.5 4,489 - -0.4 4,489 - +0.3 4,489 - +0.1 4,489 - +0.5 4,489 - +0.9 4,489 - -0.5 4,489 - +0.0 4,489 - -0.6 4,489 - -0.2 4,489 - -1.6 4,533 + LRK55_RS18360 0.14 +0.8 4,533 + LRK55_RS18360 0.14 +0.3 4,533 + LRK55_RS18360 0.14 +0.4 4,534 - LRK55_RS18360 0.14 -1.9 4,534 - LRK55_RS18360 0.14 -0.9 4,534 - LRK55_RS18360 0.14 -1.6 4,534 - LRK55_RS18360 0.14 -1.2 4,578 - LRK55_RS18360 0.18 -0.1 4,677 + LRK55_RS18360 0.28 +1.6 4,677 + LRK55_RS18360 0.28 -1.9 4,678 - LRK55_RS18360 0.28 -1.0 4,678 - LRK55_RS18360 0.28 -1.7 4,678 - LRK55_RS18360 0.28 -0.9 4,678 - LRK55_RS18360 0.28 -0.4 4,746 + LRK55_RS18360 0.35 +0.0 4,746 + LRK55_RS18360 0.35 -0.9 4,746 + LRK55_RS18360 0.35 -1.3 4,746 + LRK55_RS18360 0.35 +1.9 4,746 + LRK55_RS18360 0.35 -0.3 4,746 + LRK55_RS18360 0.35 +0.8 4,746 + LRK55_RS18360 0.35 +0.1 4,746 + LRK55_RS18360 0.35 -0.3 4,746 + LRK55_RS18360 0.35 -0.8 4,746 + LRK55_RS18360 0.35 -0.3 4,746 + LRK55_RS18360 0.35 -0.1 4,746 + LRK55_RS18360 0.35 -1.6 4,746 + LRK55_RS18360 0.35 -0.5 4,746 + LRK55_RS18360 0.35 +0.0 4,746 + LRK55_RS18360 0.35 -1.1 4,746 + LRK55_RS18360 0.35 +0.6 4,746 + LRK55_RS18360 0.35 +0.4 4,746 + LRK55_RS18360 0.35 -0.4 4,747 - LRK55_RS18360 0.35 -1.9 4,747 - LRK55_RS18360 0.35 -1.5 4,747 - LRK55_RS18360 0.35 -0.1 4,747 - LRK55_RS18360 0.35 -1.1 4,747 - LRK55_RS18360 0.35 -2.2 4,747 - LRK55_RS18360 0.35 -0.6 4,747 - LRK55_RS18360 0.35 +0.5 4,747 - LRK55_RS18360 0.35 +0.4 4,747 - LRK55_RS18360 0.35 -3.0 4,747 - LRK55_RS18360 0.35 -0.5 4,747 - LRK55_RS18360 0.35 -0.5 4,747 - LRK55_RS18360 0.35 -0.3 4,747 - LRK55_RS18360 0.35 -0.4 4,747 - LRK55_RS18360 0.35 -2.8 4,747 - LRK55_RS18360 0.35 -1.9 4,747 - LRK55_RS18360 0.35 -1.5 4,747 - LRK55_RS18360 0.35 +0.1 4,747 - LRK55_RS18360 0.35 -1.7 4,859 - LRK55_RS18360 0.47 -0.1 4,859 - LRK55_RS18360 0.47 -1.9 4,869 + LRK55_RS18360 0.48 -0.1 4,869 + LRK55_RS18360 0.48 +0.6 4,869 + LRK55_RS18360 0.48 -0.7 4,869 + LRK55_RS18360 0.48 -0.9 4,869 + LRK55_RS18360 0.48 -0.5 4,870 - LRK55_RS18360 0.48 -0.9 4,938 + LRK55_RS18360 0.55 -0.3 4,938 + LRK55_RS18360 0.55 +0.3 4,938 + LRK55_RS18360 0.55 -0.6 4,938 + LRK55_RS18360 0.55 -0.0 4,938 + LRK55_RS18360 0.55 +0.4 4,938 + LRK55_RS18360 0.55 +0.3 4,938 + LRK55_RS18360 0.55 +1.0 4,938 + LRK55_RS18360 0.55 -0.8 4,938 + LRK55_RS18360 0.55 -1.3 4,939 - LRK55_RS18360 0.55 +0.5 4,939 - LRK55_RS18360 0.55 -0.1 4,939 - LRK55_RS18360 0.55 -0.1 4,939 - LRK55_RS18360 0.55 -0.7 5,118 + LRK55_RS18360 0.73 -1.7 5,118 + LRK55_RS18360 0.73 -1.2 5,118 + LRK55_RS18360 0.73 -0.4 5,118 + LRK55_RS18360 0.73 +0.5 5,118 + LRK55_RS18360 0.73 +0.3 5,118 + LRK55_RS18360 0.73 +0.5 5,118 + LRK55_RS18360 0.73 +1.1 5,118 + LRK55_RS18360 0.73 -0.5 5,118 + LRK55_RS18360 0.73 -0.6 5,118 + LRK55_RS18360 0.73 -2.7 5,118 + LRK55_RS18360 0.73 -0.7 5,118 + LRK55_RS18360 0.73 +1.0 5,119 - LRK55_RS18360 0.73 -1.0 5,119 - LRK55_RS18360 0.73 -0.1 5,119 - LRK55_RS18360 0.73 -0.1 5,119 - LRK55_RS18360 0.73 -2.0 5,119 - LRK55_RS18360 0.73 -0.7 5,119 - LRK55_RS18360 0.73 -1.2 5,119 - LRK55_RS18360 0.73 -0.1 5,119 - LRK55_RS18360 0.73 +1.1 5,119 - LRK55_RS18360 0.73 -0.2 5,119 - LRK55_RS18360 0.73 -0.1 5,304 + -1.3 5,304 + +0.1 5,304 + -1.6 5,304 + -1.9 5,304 + -1.3 5,305 - +1.9 5,305 - -2.2 5,374 + +1.9 5,374 + +0.0 5,374 + -0.9 5,374 + +0.1 5,374 + -0.0 5,374 + +0.5 5,374 + +0.9 5,374 + -2.3 5,374 + +0.9 5,374 + -0.8 5,375 - +0.4 5,375 - +0.5 5,375 - +0.8 5,375 - -1.8 5,375 - -0.1 5,375 - -0.6 5,431 + -1.2 5,431 + -0.5 5,431 + -0.5 5,431 + -0.4 5,431 + -0.3 5,431 + +0.4 5,431 + +0.8 5,431 + +0.5 5,431 + +4.5 5,431 + +0.3 5,432 - -0.2 5,432 - -0.5 5,494 + -0.2 5,495 - +0.1 5,495 - +0.4 5,495 - +0.5 5,520 - LRK55_RS18365 0.12 -0.6 5,545 + LRK55_RS18365 0.14 -0.8 5,545 + LRK55_RS18365 0.14 -1.0 5,545 + LRK55_RS18365 0.14 +0.5 5,546 - LRK55_RS18365 0.14 -3.1 5,546 - LRK55_RS18365 0.14 -0.6 5,546 - LRK55_RS18365 0.14 -0.2 5,546 - LRK55_RS18365 0.14 -0.3 5,570 + LRK55_RS18365 0.16 +2.6 5,647 + LRK55_RS18365 0.22 -0.8 5,647 + LRK55_RS18365 0.22 -0.4 5,647 + LRK55_RS18365 0.22 -0.2 5,648 - LRK55_RS18365 0.22 +0.4 5,648 - LRK55_RS18365 0.22 -0.5 5,677 + LRK55_RS18365 0.24 +0.2 5,677 + LRK55_RS18365 0.24 -0.9 5,803 + LRK55_RS18365 0.35 +1.4 5,928 + LRK55_RS18365 0.45 -2.3 5,928 + LRK55_RS18365 0.45 +0.5 5,928 + LRK55_RS18365 0.45 -0.3 5,928 + LRK55_RS18365 0.45 +0.7 6,087 + LRK55_RS18365 0.57 -0.7 6,087 + LRK55_RS18365 0.57 -0.3 6,087 + LRK55_RS18365 0.57 -0.2 6,087 + LRK55_RS18365 0.57 -2.7 6,087 + LRK55_RS18365 0.57 -0.7 6,088 - LRK55_RS18365 0.57 +0.3 6,088 - LRK55_RS18365 0.57 +0.2 6,088 - LRK55_RS18365 0.57 -0.1 6,088 - LRK55_RS18365 0.57 +0.5 6,088 - LRK55_RS18365 0.57 -0.8
Or see this region's nucleotide sequence