Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS03580 and LRK55_RS03585 are separated by 150 nucleotides LRK55_RS03585 and LRK55_RS03590 overlap by 4 nucleotides LRK55_RS03590 and LRK55_RS03595 are separated by 3 nucleotides LRK55_RS03595 and LRK55_RS03600 are separated by 237 nucleotides
LRK55_RS03580: LRK55_RS03580 - NAD(P) transhydrogenase subunit alpha, at 755,601 to 755,906
_RS03580
LRK55_RS03585: LRK55_RS03585 - RNA polymerase sigma factor, at 756,057 to 756,641
_RS03585
LRK55_RS03590: LRK55_RS03590 - hypothetical protein, at 756,638 to 757,018
_RS03590
LRK55_RS03595: LRK55_RS03595 - DUF3106 domain-containing protein, at 757,022 to 757,549
_RS03595
LRK55_RS03600: LRK55_RS03600 - NAD(P) transhydrogenase subunit alpha, at 757,787 to 758,890
_RS03600
Position (kb) 756
757
758 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 755.672 kb on - strand, within LRK55_RS03580 at 755.851 kb on + strand, within LRK55_RS03580 at 755.859 kb on - strand, within LRK55_RS03580 at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.956 kb on + strand at 755.956 kb on + strand at 755.956 kb on + strand at 755.956 kb on + strand at 755.956 kb on + strand at 755.957 kb on - strand at 755.957 kb on - strand at 755.957 kb on - strand at 755.957 kb on - strand at 755.957 kb on - strand at 755.957 kb on - strand at 756.243 kb on + strand, within LRK55_RS03585 at 756.243 kb on + strand, within LRK55_RS03585 at 756.244 kb on - strand, within LRK55_RS03585 at 756.453 kb on + strand, within LRK55_RS03585 at 756.453 kb on + strand, within LRK55_RS03585 at 756.454 kb on - strand, within LRK55_RS03585 at 756.957 kb on - strand, within LRK55_RS03590 at 756.974 kb on + strand, within LRK55_RS03590 at 756.975 kb on - strand, within LRK55_RS03590 at 757.456 kb on + strand, within LRK55_RS03595 at 757.457 kb on - strand, within LRK55_RS03595 at 757.591 kb on + strand at 757.592 kb on - strand at 757.592 kb on - strand at 757.673 kb on + strand at 757.673 kb on + strand at 757.673 kb on + strand at 757.673 kb on + strand at 757.673 kb on + strand at 757.673 kb on + strand at 757.673 kb on + strand at 757.674 kb on - strand at 757.788 kb on + strand at 757.788 kb on + strand at 757.788 kb on + strand at 757.788 kb on + strand at 757.788 kb on + strand at 757.789 kb on - strand at 757.789 kb on - strand at 757.789 kb on - strand at 757.789 kb on - strand at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.918 kb on - strand, within LRK55_RS03600 at 757.918 kb on - strand, within LRK55_RS03600 at 757.918 kb on - strand, within LRK55_RS03600 at 757.918 kb on - strand, within LRK55_RS03600 at 757.918 kb on - strand, within LRK55_RS03600
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 755,672 - LRK55_RS03580 0.23 +1.7 755,851 + LRK55_RS03580 0.82 +2.4 755,859 - LRK55_RS03580 0.84 -1.3 755,881 + +0.7 755,881 + -0.3 755,881 + -0.5 755,881 + +0.5 755,881 + +1.0 755,881 + +0.1 755,881 + -0.2 755,881 + -0.6 755,881 + -0.5 755,881 + -0.5 755,881 + +0.6 755,881 + +1.2 755,881 + -1.0 755,882 - +0.5 755,882 - -0.2 755,882 - -0.0 755,882 - -2.1 755,882 - -0.8 755,882 - +0.1 755,882 - +0.5 755,882 - -2.8 755,882 - -1.6 755,882 - -1.2 755,882 - +0.5 755,882 - -1.6 755,882 - -0.2 755,882 - -0.5 755,956 + +0.1 755,956 + +0.6 755,956 + -0.3 755,956 + -2.6 755,956 + -0.0 755,957 - +0.6 755,957 - +0.8 755,957 - +1.0 755,957 - +0.3 755,957 - -1.0 755,957 - -0.9 756,243 + LRK55_RS03585 0.32 +0.4 756,243 + LRK55_RS03585 0.32 +0.6 756,244 - LRK55_RS03585 0.32 -0.3 756,453 + LRK55_RS03585 0.68 +1.3 756,453 + LRK55_RS03585 0.68 -0.4 756,454 - LRK55_RS03585 0.68 +0.4 756,957 - LRK55_RS03590 0.84 -2.3 756,974 + LRK55_RS03590 0.88 +1.0 756,975 - LRK55_RS03590 0.88 -1.0 757,456 + LRK55_RS03595 0.82 -2.6 757,457 - LRK55_RS03595 0.82 -0.0 757,591 + +0.0 757,592 - +0.3 757,592 - +0.0 757,673 + -0.0 757,673 + +0.5 757,673 + +1.0 757,673 + -0.0 757,673 + -0.2 757,673 + -1.6 757,673 + +1.3 757,674 - -0.2 757,788 + +0.7 757,788 + -0.3 757,788 + -1.3 757,788 + +0.4 757,788 + +0.3 757,789 - +1.0 757,789 - -2.2 757,789 - +1.3 757,789 - -0.6 757,917 + LRK55_RS03600 0.12 -0.8 757,917 + LRK55_RS03600 0.12 +1.2 757,917 + LRK55_RS03600 0.12 -0.9 757,917 + LRK55_RS03600 0.12 +0.3 757,917 + LRK55_RS03600 0.12 +1.1 757,917 + LRK55_RS03600 0.12 +0.5 757,917 + LRK55_RS03600 0.12 +1.0 757,917 + LRK55_RS03600 0.12 -0.4 757,917 + LRK55_RS03600 0.12 -0.0 757,918 - LRK55_RS03600 0.12 -0.2 757,918 - LRK55_RS03600 0.12 -0.0 757,918 - LRK55_RS03600 0.12 +0.7 757,918 - LRK55_RS03600 0.12 +1.3 757,918 - LRK55_RS03600 0.12 -0.1
Or see this region's nucleotide sequence