Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS18540 and LRK55_RS18545 are separated by 62 nucleotides LRK55_RS18545 and LRK55_RS18550 overlap by 1 nucleotides LRK55_RS18550 and LRK55_RS18555 are separated by 229 nucleotides
LRK55_RS18540: LRK55_RS18540 - hypothetical protein, at 41,610 to 42,470
_RS18540
LRK55_RS18545: LRK55_RS18545 - AAA family ATPase, at 42,533 to 43,639
_RS18545
LRK55_RS18550: LRK55_RS18550 - hypothetical protein, at 43,639 to 44,295
_RS18550
LRK55_RS18555: LRK55_RS18555 - DUF3150 domain-containing protein, at 44,525 to 45,535
_RS18555
Position (kb) 42
43
44 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.536 kb on + strand at 41.537 kb on - strand at 41.537 kb on - strand at 41.537 kb on - strand at 41.537 kb on - strand at 41.537 kb on - strand at 41.537 kb on - strand at 41.537 kb on - strand at 41.537 kb on - strand at 41.537 kb on - strand at 41.537 kb on - strand at 41.681 kb on + strand at 41.681 kb on + strand at 41.681 kb on + strand at 41.682 kb on - strand at 41.923 kb on - strand, within LRK55_RS18540 at 41.942 kb on + strand, within LRK55_RS18540 at 41.942 kb on + strand, within LRK55_RS18540 at 41.942 kb on + strand, within LRK55_RS18540 at 41.942 kb on + strand, within LRK55_RS18540 at 41.942 kb on + strand, within LRK55_RS18540 at 41.942 kb on + strand, within LRK55_RS18540 at 41.942 kb on + strand, within LRK55_RS18540 at 41.942 kb on + strand, within LRK55_RS18540 at 41.942 kb on + strand, within LRK55_RS18540 at 41.942 kb on + strand, within LRK55_RS18540 at 41.942 kb on + strand, within LRK55_RS18540 at 41.942 kb on + strand, within LRK55_RS18540 at 41.942 kb on + strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.943 kb on - strand, within LRK55_RS18540 at 41.946 kb on + strand, within LRK55_RS18540 at 41.946 kb on + strand, within LRK55_RS18540 at 41.946 kb on + strand, within LRK55_RS18540 at 41.946 kb on + strand, within LRK55_RS18540 at 41.947 kb on - strand, within LRK55_RS18540 at 41.947 kb on - strand, within LRK55_RS18540 at 41.947 kb on - strand, within LRK55_RS18540 at 41.947 kb on - strand, within LRK55_RS18540 at 41.947 kb on - strand, within LRK55_RS18540 at 41.947 kb on - strand, within LRK55_RS18540 at 41.947 kb on - strand, within LRK55_RS18540 at 41.947 kb on - strand, within LRK55_RS18540 at 42.201 kb on + strand, within LRK55_RS18540 at 42.202 kb on - strand, within LRK55_RS18540 at 42.202 kb on - strand, within LRK55_RS18540 at 42.447 kb on + strand at 42.448 kb on - strand at 42.448 kb on - strand at 42.536 kb on + strand at 42.536 kb on + strand at 42.536 kb on + strand at 42.536 kb on + strand at 42.537 kb on - strand at 42.537 kb on - strand at 42.537 kb on - strand at 42.537 kb on - strand at 42.537 kb on - strand at 42.537 kb on - strand at 42.537 kb on - strand at 42.537 kb on - strand at 42.537 kb on - strand at 42.537 kb on - strand at 42.611 kb on + strand at 42.611 kb on + strand at 42.641 kb on + strand at 42.641 kb on + strand at 42.687 kb on - strand, within LRK55_RS18545 at 42.687 kb on - strand, within LRK55_RS18545 at 42.705 kb on - strand, within LRK55_RS18545 at 42.705 kb on - strand, within LRK55_RS18545 at 42.705 kb on - strand, within LRK55_RS18545 at 42.793 kb on + strand, within LRK55_RS18545 at 42.793 kb on + strand, within LRK55_RS18545 at 42.794 kb on - strand, within LRK55_RS18545 at 42.794 kb on - strand, within LRK55_RS18545 at 42.887 kb on + strand, within LRK55_RS18545 at 42.887 kb on + strand, within LRK55_RS18545 at 42.888 kb on - strand, within LRK55_RS18545 at 42.888 kb on - strand, within LRK55_RS18545 at 42.971 kb on + strand, within LRK55_RS18545 at 43.044 kb on - strand, within LRK55_RS18545 at 43.223 kb on + strand, within LRK55_RS18545 at 43.362 kb on - strand, within LRK55_RS18545 at 43.505 kb on + strand, within LRK55_RS18545 at 43.506 kb on - strand, within LRK55_RS18545 at 43.538 kb on + strand at 43.556 kb on + strand at 43.604 kb on + strand at 43.604 kb on + strand at 43.604 kb on + strand at 43.604 kb on + strand at 43.604 kb on + strand at 43.605 kb on - strand at 43.651 kb on + strand at 43.651 kb on + strand at 43.651 kb on + strand at 43.651 kb on + strand at 43.651 kb on + strand at 43.652 kb on - strand at 43.831 kb on + strand, within LRK55_RS18550 at 43.831 kb on + strand, within LRK55_RS18550 at 43.831 kb on + strand, within LRK55_RS18550 at 43.831 kb on + strand, within LRK55_RS18550 at 43.928 kb on + strand, within LRK55_RS18550 at 44.062 kb on + strand, within LRK55_RS18550 at 44.062 kb on + strand, within LRK55_RS18550 at 44.063 kb on - strand, within LRK55_RS18550 at 44.212 kb on + strand, within LRK55_RS18550 at 44.212 kb on + strand, within LRK55_RS18550 at 44.212 kb on + strand, within LRK55_RS18550 at 44.213 kb on - strand, within LRK55_RS18550 at 44.268 kb on + strand at 44.269 kb on - strand at 44.437 kb on + strand at 44.437 kb on + strand at 44.437 kb on + strand at 44.438 kb on - strand at 44.438 kb on - strand at 44.605 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 41,536 + -0.7 41,536 + +0.7 41,536 + -2.1 41,536 + -0.6 41,536 + -2.4 41,536 + +0.2 41,536 + +0.5 41,536 + +0.4 41,536 + +0.0 41,536 + -0.6 41,536 + -1.1 41,536 + +0.0 41,536 + -1.2 41,536 + +1.1 41,536 + +0.2 41,536 + +0.9 41,536 + +1.9 41,536 + -0.7 41,536 + +2.2 41,536 + +1.9 41,536 + +0.5 41,537 - +1.3 41,537 - -0.1 41,537 - +0.5 41,537 - +0.3 41,537 - -0.7 41,537 - -1.8 41,537 - -2.5 41,537 - +0.6 41,537 - +1.0 41,537 - -1.4 41,681 + -2.5 41,681 + -2.8 41,681 + +0.9 41,682 - +0.8 41,923 - LRK55_RS18540 0.36 -2.1 41,942 + LRK55_RS18540 0.39 +1.5 41,942 + LRK55_RS18540 0.39 +1.5 41,942 + LRK55_RS18540 0.39 -0.3 41,942 + LRK55_RS18540 0.39 -1.2 41,942 + LRK55_RS18540 0.39 -3.1 41,942 + LRK55_RS18540 0.39 -0.2 41,942 + LRK55_RS18540 0.39 -0.1 41,942 + LRK55_RS18540 0.39 +1.1 41,942 + LRK55_RS18540 0.39 +0.5 41,942 + LRK55_RS18540 0.39 +0.1 41,942 + LRK55_RS18540 0.39 +0.5 41,942 + LRK55_RS18540 0.39 +0.9 41,942 + LRK55_RS18540 0.39 +0.5 41,943 - LRK55_RS18540 0.39 -0.7 41,943 - LRK55_RS18540 0.39 +0.7 41,943 - LRK55_RS18540 0.39 -0.1 41,943 - LRK55_RS18540 0.39 +0.2 41,943 - LRK55_RS18540 0.39 +0.8 41,943 - LRK55_RS18540 0.39 +0.1 41,943 - LRK55_RS18540 0.39 +0.1 41,943 - LRK55_RS18540 0.39 +0.3 41,943 - LRK55_RS18540 0.39 +0.6 41,943 - LRK55_RS18540 0.39 -0.0 41,943 - LRK55_RS18540 0.39 +0.1 41,943 - LRK55_RS18540 0.39 -0.1 41,943 - LRK55_RS18540 0.39 -1.2 41,943 - LRK55_RS18540 0.39 +0.5 41,943 - LRK55_RS18540 0.39 -0.5 41,943 - LRK55_RS18540 0.39 -0.0 41,943 - LRK55_RS18540 0.39 -0.9 41,946 + LRK55_RS18540 0.39 +0.5 41,946 + LRK55_RS18540 0.39 +1.1 41,946 + LRK55_RS18540 0.39 +0.8 41,946 + LRK55_RS18540 0.39 -2.9 41,947 - LRK55_RS18540 0.39 -1.2 41,947 - LRK55_RS18540 0.39 -0.1 41,947 - LRK55_RS18540 0.39 -2.3 41,947 - LRK55_RS18540 0.39 +0.9 41,947 - LRK55_RS18540 0.39 +1.1 41,947 - LRK55_RS18540 0.39 -0.9 41,947 - LRK55_RS18540 0.39 +0.6 41,947 - LRK55_RS18540 0.39 -1.5 42,201 + LRK55_RS18540 0.69 +0.4 42,202 - LRK55_RS18540 0.69 -0.5 42,202 - LRK55_RS18540 0.69 +0.5 42,447 + +0.7 42,448 - -1.6 42,448 - -1.8 42,536 + -1.5 42,536 + +0.3 42,536 + -1.9 42,536 + +0.1 42,537 - +0.6 42,537 - -1.9 42,537 - -0.3 42,537 - -0.9 42,537 - +0.6 42,537 - -2.9 42,537 - +1.2 42,537 - +0.4 42,537 - +0.2 42,537 - +1.2 42,611 + +1.6 42,611 + +1.1 42,641 + -0.8 42,641 + +0.7 42,687 - LRK55_RS18545 0.14 +1.5 42,687 - LRK55_RS18545 0.14 +0.1 42,705 - LRK55_RS18545 0.16 -0.9 42,705 - LRK55_RS18545 0.16 +0.1 42,705 - LRK55_RS18545 0.16 -2.7 42,793 + LRK55_RS18545 0.23 -1.2 42,793 + LRK55_RS18545 0.23 -1.1 42,794 - LRK55_RS18545 0.24 +0.8 42,794 - LRK55_RS18545 0.24 +0.6 42,887 + LRK55_RS18545 0.32 -2.9 42,887 + LRK55_RS18545 0.32 +0.1 42,888 - LRK55_RS18545 0.32 -0.3 42,888 - LRK55_RS18545 0.32 -0.4 42,971 + LRK55_RS18545 0.40 +1.5 43,044 - LRK55_RS18545 0.46 +0.9 43,223 + LRK55_RS18545 0.62 -0.2 43,362 - LRK55_RS18545 0.75 -0.1 43,505 + LRK55_RS18545 0.88 +1.1 43,506 - LRK55_RS18545 0.88 -1.9 43,538 + -1.1 43,556 + +0.7 43,604 + +0.4 43,604 + +2.7 43,604 + -0.3 43,604 + -1.5 43,604 + +0.1 43,605 - +0.0 43,651 + -0.2 43,651 + +0.1 43,651 + -0.0 43,651 + +0.7 43,651 + +0.4 43,652 - +0.8 43,831 + LRK55_RS18550 0.29 -1.0 43,831 + LRK55_RS18550 0.29 -2.6 43,831 + LRK55_RS18550 0.29 +1.3 43,831 + LRK55_RS18550 0.29 -0.1 43,928 + LRK55_RS18550 0.44 -0.5 44,062 + LRK55_RS18550 0.64 -0.2 44,062 + LRK55_RS18550 0.64 +0.6 44,063 - LRK55_RS18550 0.65 -0.4 44,212 + LRK55_RS18550 0.87 -1.4 44,212 + LRK55_RS18550 0.87 +0.5 44,212 + LRK55_RS18550 0.87 -1.2 44,213 - LRK55_RS18550 0.87 +0.2 44,268 + +0.6 44,269 - +2.7 44,437 + -1.7 44,437 + +0.7 44,437 + -0.4 44,438 - +1.5 44,438 - +0.2 44,605 + +1.4
Or see this region's nucleotide sequence