Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS14345 and LRK55_RS14350 are separated by 27 nucleotides LRK55_RS14350 and LRK55_RS14355 are separated by 92 nucleotides LRK55_RS14355 and LRK55_RS14360 are separated by 0 nucleotides LRK55_RS14360 and LRK55_RS14365 are separated by 84 nucleotides
LRK55_RS14345: LRK55_RS14345 - NADPH-dependent FMN reductase, at 3,166,728 to 3,167,354
_RS14345
LRK55_RS14350: LRK55_RS14350 - LysE family translocator, at 3,167,382 to 3,167,978
_RS14350
LRK55_RS14355: LRK55_RS14355 - LysR substrate-binding domain-containing protein, at 3,168,071 to 3,168,976
_RS14355
LRK55_RS14360: LRK55_RS14360 - thioredoxin TrxC, at 3,168,977 to 3,169,420
_RS14360
LRK55_RS14365: LRK55_RS14365 - hypothetical protein, at 3,169,505 to 3,169,963
_RS14365
Position (kb)
3168
3169 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3167.089 kb on + strand, within LRK55_RS14345 at 3167.089 kb on + strand, within LRK55_RS14345 at 3167.089 kb on + strand, within LRK55_RS14345 at 3167.089 kb on + strand, within LRK55_RS14345 at 3167.090 kb on - strand, within LRK55_RS14345 at 3167.090 kb on - strand, within LRK55_RS14345 at 3167.221 kb on + strand, within LRK55_RS14345 at 3167.221 kb on + strand, within LRK55_RS14345 at 3167.221 kb on + strand, within LRK55_RS14345 at 3167.221 kb on + strand, within LRK55_RS14345 at 3167.339 kb on + strand at 3167.339 kb on + strand at 3167.340 kb on - strand at 3167.340 kb on - strand at 3167.340 kb on - strand at 3167.340 kb on - strand at 3167.340 kb on - strand at 3167.340 kb on - strand at 3167.340 kb on - strand at 3167.360 kb on + strand at 3167.557 kb on + strand, within LRK55_RS14350 at 3167.557 kb on + strand, within LRK55_RS14350 at 3167.558 kb on - strand, within LRK55_RS14350 at 3167.721 kb on + strand, within LRK55_RS14350 at 3167.722 kb on - strand, within LRK55_RS14350 at 3167.722 kb on - strand, within LRK55_RS14350 at 3167.740 kb on + strand, within LRK55_RS14350 at 3167.740 kb on + strand, within LRK55_RS14350 at 3167.741 kb on - strand, within LRK55_RS14350 at 3167.741 kb on - strand, within LRK55_RS14350 at 3167.741 kb on - strand, within LRK55_RS14350 at 3167.741 kb on - strand, within LRK55_RS14350 at 3167.741 kb on - strand, within LRK55_RS14350 at 3167.773 kb on - strand, within LRK55_RS14350 at 3168.058 kb on + strand at 3168.116 kb on + strand at 3168.116 kb on + strand at 3168.116 kb on + strand at 3168.116 kb on + strand at 3168.116 kb on + strand at 3168.116 kb on + strand at 3168.117 kb on - strand at 3168.117 kb on - strand at 3168.117 kb on - strand at 3168.132 kb on + strand at 3168.132 kb on + strand at 3168.133 kb on - strand at 3168.133 kb on - strand at 3168.133 kb on - strand at 3168.566 kb on + strand, within LRK55_RS14355 at 3168.566 kb on + strand, within LRK55_RS14355 at 3168.566 kb on + strand, within LRK55_RS14355 at 3168.567 kb on - strand, within LRK55_RS14355 at 3168.731 kb on + strand, within LRK55_RS14355 at 3168.731 kb on + strand, within LRK55_RS14355 at 3168.731 kb on + strand, within LRK55_RS14355 at 3168.731 kb on + strand, within LRK55_RS14355 at 3168.732 kb on - strand, within LRK55_RS14355 at 3168.732 kb on - strand, within LRK55_RS14355 at 3168.876 kb on - strand, within LRK55_RS14355 at 3168.876 kb on - strand, within LRK55_RS14355 at 3168.876 kb on - strand, within LRK55_RS14355 at 3168.876 kb on - strand, within LRK55_RS14355 at 3168.876 kb on - strand, within LRK55_RS14355 at 3169.414 kb on + strand at 3169.414 kb on + strand at 3169.414 kb on + strand at 3169.414 kb on + strand at 3169.415 kb on - strand at 3169.415 kb on - strand at 3169.415 kb on - strand at 3169.415 kb on - strand at 3169.415 kb on - strand at 3169.415 kb on - strand at 3169.415 kb on - strand at 3169.415 kb on - strand at 3169.415 kb on - strand at 3169.588 kb on + strand, within LRK55_RS14365 at 3169.766 kb on + strand, within LRK55_RS14365 at 3169.766 kb on + strand, within LRK55_RS14365 at 3169.766 kb on + strand, within LRK55_RS14365 at 3169.766 kb on + strand, within LRK55_RS14365 at 3169.767 kb on - strand, within LRK55_RS14365 at 3169.767 kb on - strand, within LRK55_RS14365 at 3169.767 kb on - strand, within LRK55_RS14365 at 3169.767 kb on - strand, within LRK55_RS14365 at 3169.835 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.841 kb on + strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.842 kb on - strand, within LRK55_RS14365 at 3169.874 kb on + strand, within LRK55_RS14365 at 3169.875 kb on - strand, within LRK55_RS14365 at 3169.875 kb on - strand, within LRK55_RS14365 at 3169.875 kb on - strand, within LRK55_RS14365 at 3169.875 kb on - strand, within LRK55_RS14365 at 3169.875 kb on - strand, within LRK55_RS14365 at 3169.932 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 3,167,089 + LRK55_RS14345 0.58 +0.5 3,167,089 + LRK55_RS14345 0.58 +0.5 3,167,089 + LRK55_RS14345 0.58 -1.4 3,167,089 + LRK55_RS14345 0.58 +0.7 3,167,090 - LRK55_RS14345 0.58 -0.3 3,167,090 - LRK55_RS14345 0.58 -1.0 3,167,221 + LRK55_RS14345 0.79 -0.0 3,167,221 + LRK55_RS14345 0.79 +1.4 3,167,221 + LRK55_RS14345 0.79 +0.7 3,167,221 + LRK55_RS14345 0.79 -0.1 3,167,339 + +1.3 3,167,339 + -0.6 3,167,340 - +1.0 3,167,340 - -0.5 3,167,340 - -1.0 3,167,340 - -0.2 3,167,340 - +0.2 3,167,340 - +1.4 3,167,340 - +0.6 3,167,360 + +0.2 3,167,557 + LRK55_RS14350 0.29 +0.1 3,167,557 + LRK55_RS14350 0.29 +0.6 3,167,558 - LRK55_RS14350 0.29 -2.1 3,167,721 + LRK55_RS14350 0.57 +0.1 3,167,722 - LRK55_RS14350 0.57 -0.1 3,167,722 - LRK55_RS14350 0.57 +0.0 3,167,740 + LRK55_RS14350 0.60 -0.2 3,167,740 + LRK55_RS14350 0.60 +0.9 3,167,741 - LRK55_RS14350 0.60 +0.0 3,167,741 - LRK55_RS14350 0.60 -1.2 3,167,741 - LRK55_RS14350 0.60 -0.4 3,167,741 - LRK55_RS14350 0.60 -0.7 3,167,741 - LRK55_RS14350 0.60 +0.2 3,167,773 - LRK55_RS14350 0.65 +1.6 3,168,058 + -2.1 3,168,116 + +1.9 3,168,116 + +0.4 3,168,116 + +0.5 3,168,116 + +0.5 3,168,116 + +0.8 3,168,116 + +0.9 3,168,117 - -1.0 3,168,117 - +0.2 3,168,117 - +0.0 3,168,132 + +0.4 3,168,132 + -0.3 3,168,133 - +0.6 3,168,133 - -0.0 3,168,133 - -0.6 3,168,566 + LRK55_RS14355 0.55 +1.1 3,168,566 + LRK55_RS14355 0.55 -0.9 3,168,566 + LRK55_RS14355 0.55 -0.6 3,168,567 - LRK55_RS14355 0.55 -1.7 3,168,731 + LRK55_RS14355 0.73 +1.1 3,168,731 + LRK55_RS14355 0.73 +0.6 3,168,731 + LRK55_RS14355 0.73 +0.3 3,168,731 + LRK55_RS14355 0.73 +0.6 3,168,732 - LRK55_RS14355 0.73 -0.7 3,168,732 - LRK55_RS14355 0.73 -2.5 3,168,876 - LRK55_RS14355 0.89 -1.8 3,168,876 - LRK55_RS14355 0.89 -0.9 3,168,876 - LRK55_RS14355 0.89 +1.9 3,168,876 - LRK55_RS14355 0.89 +1.4 3,168,876 - LRK55_RS14355 0.89 +0.6 3,169,414 + -0.5 3,169,414 + -1.7 3,169,414 + -0.2 3,169,414 + -0.2 3,169,415 - -0.0 3,169,415 - -0.9 3,169,415 - -1.7 3,169,415 - +0.5 3,169,415 - +1.1 3,169,415 - +1.0 3,169,415 - +2.7 3,169,415 - -2.8 3,169,415 - -1.1 3,169,588 + LRK55_RS14365 0.18 +0.0 3,169,766 + LRK55_RS14365 0.57 -1.9 3,169,766 + LRK55_RS14365 0.57 +0.9 3,169,766 + LRK55_RS14365 0.57 -0.8 3,169,766 + LRK55_RS14365 0.57 -0.9 3,169,767 - LRK55_RS14365 0.57 -0.5 3,169,767 - LRK55_RS14365 0.57 -0.7 3,169,767 - LRK55_RS14365 0.57 -1.6 3,169,767 - LRK55_RS14365 0.57 -1.4 3,169,835 + LRK55_RS14365 0.72 +0.7 3,169,841 + LRK55_RS14365 0.73 +0.9 3,169,841 + LRK55_RS14365 0.73 -0.1 3,169,841 + LRK55_RS14365 0.73 +0.9 3,169,841 + LRK55_RS14365 0.73 -0.8 3,169,841 + LRK55_RS14365 0.73 -2.0 3,169,841 + LRK55_RS14365 0.73 +0.1 3,169,841 + LRK55_RS14365 0.73 +0.5 3,169,841 + LRK55_RS14365 0.73 -0.3 3,169,841 + LRK55_RS14365 0.73 -1.7 3,169,841 + LRK55_RS14365 0.73 +0.4 3,169,841 + LRK55_RS14365 0.73 +2.2 3,169,841 + LRK55_RS14365 0.73 +0.0 3,169,841 + LRK55_RS14365 0.73 -2.5 3,169,841 + LRK55_RS14365 0.73 +0.8 3,169,841 + LRK55_RS14365 0.73 -1.2 3,169,841 + LRK55_RS14365 0.73 -0.1 3,169,842 - LRK55_RS14365 0.73 +0.4 3,169,842 - LRK55_RS14365 0.73 +0.1 3,169,842 - LRK55_RS14365 0.73 +0.1 3,169,842 - LRK55_RS14365 0.73 +1.3 3,169,842 - LRK55_RS14365 0.73 -1.1 3,169,842 - LRK55_RS14365 0.73 +1.6 3,169,842 - LRK55_RS14365 0.73 +0.5 3,169,842 - LRK55_RS14365 0.73 -1.1 3,169,842 - LRK55_RS14365 0.73 -1.4 3,169,842 - LRK55_RS14365 0.73 +0.7 3,169,842 - LRK55_RS14365 0.73 -0.1 3,169,842 - LRK55_RS14365 0.73 -2.5 3,169,842 - LRK55_RS14365 0.73 -0.7 3,169,842 - LRK55_RS14365 0.73 -0.2 3,169,842 - LRK55_RS14365 0.73 -0.0 3,169,874 + LRK55_RS14365 0.80 -2.8 3,169,875 - LRK55_RS14365 0.81 -1.1 3,169,875 - LRK55_RS14365 0.81 +0.0 3,169,875 - LRK55_RS14365 0.81 -1.1 3,169,875 - LRK55_RS14365 0.81 +0.1 3,169,875 - LRK55_RS14365 0.81 -0.9 3,169,932 - +1.2
Or see this region's nucleotide sequence