Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS09885 and LRK55_RS09890 overlap by 4 nucleotides LRK55_RS09890 and LRK55_RS09895 are separated by 28 nucleotides LRK55_RS09895 and LRK55_RS09900 overlap by 14 nucleotides
LRK55_RS09885: LRK55_RS09885 - pimeloyl-ACP methyl ester esterase BioH, at 2,167,480 to 2,168,265
_RS09885
LRK55_RS09890: LRK55_RS09890 - sulfurtransferase, at 2,168,262 to 2,169,170
_RS09890
LRK55_RS09895: LRK55_RS09895 - 8-amino-7-oxononanoate synthase, at 2,169,199 to 2,170,404
_RS09895
LRK55_RS09900: LRK55_RS09900 - biotin synthase BioB, at 2,170,391 to 2,171,434
_RS09900
Position (kb)
2169
2170
2171 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2168.255 kb on + strand at 2168.255 kb on + strand at 2168.255 kb on + strand at 2168.255 kb on + strand at 2168.256 kb on - strand at 2168.256 kb on - strand at 2168.256 kb on - strand at 2168.256 kb on - strand at 2168.256 kb on - strand at 2168.533 kb on + strand, within LRK55_RS09890 at 2168.534 kb on - strand, within LRK55_RS09890 at 2168.534 kb on - strand, within LRK55_RS09890 at 2168.534 kb on - strand, within LRK55_RS09890 at 2168.534 kb on - strand, within LRK55_RS09890 at 2168.534 kb on - strand, within LRK55_RS09890 at 2168.560 kb on + strand, within LRK55_RS09890 at 2168.560 kb on + strand, within LRK55_RS09890 at 2168.560 kb on + strand, within LRK55_RS09890 at 2168.561 kb on - strand, within LRK55_RS09890 at 2168.561 kb on - strand, within LRK55_RS09890 at 2168.629 kb on + strand, within LRK55_RS09890 at 2168.630 kb on - strand, within LRK55_RS09890 at 2168.630 kb on - strand, within LRK55_RS09890 at 2168.630 kb on - strand, within LRK55_RS09890 at 2168.749 kb on + strand, within LRK55_RS09890 at 2168.749 kb on + strand, within LRK55_RS09890 at 2168.749 kb on + strand, within LRK55_RS09890 at 2169.146 kb on - strand at 2169.146 kb on - strand at 2169.201 kb on - strand at 2169.201 kb on - strand at 2169.202 kb on + strand at 2169.202 kb on + strand at 2169.202 kb on + strand at 2169.202 kb on + strand at 2169.202 kb on + strand at 2169.202 kb on + strand at 2169.202 kb on + strand at 2169.203 kb on - strand at 2169.203 kb on - strand at 2169.203 kb on - strand at 2169.203 kb on - strand at 2169.203 kb on - strand at 2169.596 kb on + strand, within LRK55_RS09895 at 2169.596 kb on + strand, within LRK55_RS09895 at 2169.596 kb on + strand, within LRK55_RS09895 at 2169.596 kb on + strand, within LRK55_RS09895 at 2169.597 kb on - strand, within LRK55_RS09895 at 2169.597 kb on - strand, within LRK55_RS09895 at 2169.597 kb on - strand, within LRK55_RS09895 at 2169.597 kb on - strand, within LRK55_RS09895 at 2169.597 kb on - strand, within LRK55_RS09895 at 2169.597 kb on - strand, within LRK55_RS09895 at 2169.602 kb on + strand, within LRK55_RS09895 at 2169.602 kb on + strand, within LRK55_RS09895 at 2169.603 kb on - strand, within LRK55_RS09895 at 2169.603 kb on - strand, within LRK55_RS09895 at 2169.603 kb on - strand, within LRK55_RS09895 at 2169.603 kb on - strand, within LRK55_RS09895 at 2169.603 kb on - strand, within LRK55_RS09895 at 2170.071 kb on - strand, within LRK55_RS09895 at 2170.071 kb on - strand, within LRK55_RS09895 at 2170.071 kb on - strand, within LRK55_RS09895 at 2170.251 kb on - strand, within LRK55_RS09895 at 2170.465 kb on + strand at 2170.466 kb on - strand at 2170.944 kb on + strand, within LRK55_RS09900 at 2170.944 kb on + strand, within LRK55_RS09900 at 2170.944 kb on + strand, within LRK55_RS09900 at 2170.945 kb on - strand, within LRK55_RS09900 at 2170.977 kb on + strand, within LRK55_RS09900 at 2170.977 kb on + strand, within LRK55_RS09900 at 2170.977 kb on + strand, within LRK55_RS09900 at 2170.977 kb on + strand, within LRK55_RS09900 at 2170.977 kb on + strand, within LRK55_RS09900 at 2170.977 kb on + strand, within LRK55_RS09900 at 2170.977 kb on + strand, within LRK55_RS09900 at 2170.978 kb on - strand, within LRK55_RS09900 at 2170.978 kb on - strand, within LRK55_RS09900 at 2170.978 kb on - strand, within LRK55_RS09900 at 2170.978 kb on - strand, within LRK55_RS09900 at 2170.980 kb on + strand, within LRK55_RS09900 at 2170.980 kb on + strand, within LRK55_RS09900 at 2170.981 kb on - strand, within LRK55_RS09900 at 2170.981 kb on - strand, within LRK55_RS09900 at 2171.251 kb on - strand, within LRK55_RS09900 at 2171.251 kb on - strand, within LRK55_RS09900
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 2,168,255 + +2.4 2,168,255 + -3.1 2,168,255 + +1.3 2,168,255 + -1.1 2,168,256 - +0.3 2,168,256 - +0.7 2,168,256 - +1.7 2,168,256 - -0.3 2,168,256 - -0.6 2,168,533 + LRK55_RS09890 0.30 +0.5 2,168,534 - LRK55_RS09890 0.30 +0.1 2,168,534 - LRK55_RS09890 0.30 +1.0 2,168,534 - LRK55_RS09890 0.30 +3.3 2,168,534 - LRK55_RS09890 0.30 -0.3 2,168,534 - LRK55_RS09890 0.30 -1.8 2,168,560 + LRK55_RS09890 0.33 -0.8 2,168,560 + LRK55_RS09890 0.33 -0.7 2,168,560 + LRK55_RS09890 0.33 -0.2 2,168,561 - LRK55_RS09890 0.33 +0.1 2,168,561 - LRK55_RS09890 0.33 -0.8 2,168,629 + LRK55_RS09890 0.40 +0.4 2,168,630 - LRK55_RS09890 0.40 +0.3 2,168,630 - LRK55_RS09890 0.40 +0.8 2,168,630 - LRK55_RS09890 0.40 +0.5 2,168,749 + LRK55_RS09890 0.54 +0.2 2,168,749 + LRK55_RS09890 0.54 -1.1 2,168,749 + LRK55_RS09890 0.54 -1.6 2,169,146 - +0.1 2,169,146 - +0.1 2,169,201 - +0.3 2,169,201 - -0.1 2,169,202 + -0.2 2,169,202 + -0.4 2,169,202 + +0.1 2,169,202 + -0.1 2,169,202 + -1.9 2,169,202 + +0.7 2,169,202 + -0.1 2,169,203 - -0.2 2,169,203 - -0.0 2,169,203 - +0.2 2,169,203 - +0.1 2,169,203 - -1.0 2,169,596 + LRK55_RS09895 0.33 -1.8 2,169,596 + LRK55_RS09895 0.33 -1.5 2,169,596 + LRK55_RS09895 0.33 -0.3 2,169,596 + LRK55_RS09895 0.33 +0.3 2,169,597 - LRK55_RS09895 0.33 -1.2 2,169,597 - LRK55_RS09895 0.33 -2.0 2,169,597 - LRK55_RS09895 0.33 -3.5 2,169,597 - LRK55_RS09895 0.33 -3.2 2,169,597 - LRK55_RS09895 0.33 -0.2 2,169,597 - LRK55_RS09895 0.33 +0.6 2,169,602 + LRK55_RS09895 0.33 +0.9 2,169,602 + LRK55_RS09895 0.33 -3.0 2,169,603 - LRK55_RS09895 0.33 +0.9 2,169,603 - LRK55_RS09895 0.33 -0.4 2,169,603 - LRK55_RS09895 0.33 -0.4 2,169,603 - LRK55_RS09895 0.33 -1.3 2,169,603 - LRK55_RS09895 0.33 +3.0 2,170,071 - LRK55_RS09895 0.72 +1.5 2,170,071 - LRK55_RS09895 0.72 +1.6 2,170,071 - LRK55_RS09895 0.72 +1.9 2,170,251 - LRK55_RS09895 0.87 -0.4 2,170,465 + +0.2 2,170,466 - +0.4 2,170,944 + LRK55_RS09900 0.53 -0.9 2,170,944 + LRK55_RS09900 0.53 +0.7 2,170,944 + LRK55_RS09900 0.53 -1.1 2,170,945 - LRK55_RS09900 0.53 -0.8 2,170,977 + LRK55_RS09900 0.56 +1.5 2,170,977 + LRK55_RS09900 0.56 -1.9 2,170,977 + LRK55_RS09900 0.56 +0.6 2,170,977 + LRK55_RS09900 0.56 +0.8 2,170,977 + LRK55_RS09900 0.56 +0.7 2,170,977 + LRK55_RS09900 0.56 +1.0 2,170,977 + LRK55_RS09900 0.56 -2.4 2,170,978 - LRK55_RS09900 0.56 -0.1 2,170,978 - LRK55_RS09900 0.56 +0.9 2,170,978 - LRK55_RS09900 0.56 -0.1 2,170,978 - LRK55_RS09900 0.56 +1.1 2,170,980 + LRK55_RS09900 0.56 +0.6 2,170,980 + LRK55_RS09900 0.56 +0.3 2,170,981 - LRK55_RS09900 0.57 -2.6 2,170,981 - LRK55_RS09900 0.57 +0.1 2,171,251 - LRK55_RS09900 0.82 -0.4 2,171,251 - LRK55_RS09900 0.82 +1.6
Or see this region's nucleotide sequence