Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS08995 and LRK55_RS09000 are separated by 9 nucleotides LRK55_RS09000 and LRK55_RS09005 are separated by 268 nucleotides LRK55_RS09005 and LRK55_RS09010 overlap by 11 nucleotides LRK55_RS09010 and LRK55_RS09015 overlap by 4 nucleotides
LRK55_RS08995: LRK55_RS08995 - IS3 family transposase, at 1,971,444 to 1,972,459
_RS08995
LRK55_RS09000: LRK55_RS09000 - hypothetical protein, at 1,972,469 to 1,972,729
_RS09000
LRK55_RS09005: LRK55_RS09005 - AIPR family protein, at 1,972,998 to 1,974,791
_RS09005
LRK55_RS09010: LRK55_RS09010 - PD-(D/E)XK motif protein, at 1,974,781 to 1,975,776
_RS09010
LRK55_RS09015: LRK55_RS09015 - Z1 domain-containing protein, at 1,975,773 to 1,978,667
_RS09015
Position (kb) 1972
1973
1974
1975 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1972.470 kb on + strand at 1972.556 kb on + strand, within LRK55_RS09000 at 1972.556 kb on + strand, within LRK55_RS09000 at 1972.556 kb on + strand, within LRK55_RS09000 at 1972.556 kb on + strand, within LRK55_RS09000 at 1972.556 kb on + strand, within LRK55_RS09000 at 1972.556 kb on + strand, within LRK55_RS09000 at 1972.556 kb on + strand, within LRK55_RS09000 at 1972.556 kb on + strand, within LRK55_RS09000 at 1972.557 kb on - strand, within LRK55_RS09000 at 1972.557 kb on - strand, within LRK55_RS09000 at 1972.557 kb on - strand, within LRK55_RS09000 at 1972.622 kb on + strand, within LRK55_RS09000 at 1972.654 kb on + strand, within LRK55_RS09000 at 1972.654 kb on + strand, within LRK55_RS09000 at 1972.654 kb on + strand, within LRK55_RS09000 at 1972.655 kb on - strand, within LRK55_RS09000 at 1972.655 kb on - strand, within LRK55_RS09000 at 1972.733 kb on + strand at 1972.733 kb on + strand at 1972.733 kb on + strand at 1972.733 kb on + strand at 1972.733 kb on + strand at 1972.733 kb on + strand at 1972.734 kb on - strand at 1972.734 kb on - strand at 1972.734 kb on - strand at 1972.734 kb on - strand at 1972.737 kb on + strand at 1972.737 kb on + strand at 1972.737 kb on + strand at 1972.737 kb on + strand at 1972.737 kb on + strand at 1972.737 kb on + strand at 1972.738 kb on - strand at 1972.738 kb on - strand at 1972.738 kb on - strand at 1972.738 kb on - strand at 1972.738 kb on - strand at 1972.738 kb on - strand at 1972.738 kb on - strand at 1972.738 kb on - strand at 1972.738 kb on - strand at 1972.738 kb on - strand at 1972.786 kb on + strand at 1972.786 kb on + strand at 1972.786 kb on + strand at 1972.786 kb on + strand at 1972.787 kb on - strand at 1972.787 kb on - strand at 1972.787 kb on - strand at 1972.808 kb on + strand at 1972.809 kb on - strand at 1972.872 kb on + strand at 1972.873 kb on - strand at 1972.880 kb on + strand at 1972.880 kb on + strand at 1972.881 kb on - strand at 1972.923 kb on + strand at 1972.924 kb on - strand at 1972.924 kb on - strand at 1972.952 kb on - strand at 1972.971 kb on + strand at 1973.046 kb on + strand at 1973.046 kb on + strand at 1973.047 kb on - strand at 1973.047 kb on - strand at 1973.047 kb on - strand at 1973.047 kb on - strand at 1973.105 kb on + strand at 1973.105 kb on + strand at 1973.106 kb on - strand at 1973.188 kb on - strand, within LRK55_RS09005 at 1973.215 kb on + strand, within LRK55_RS09005 at 1973.215 kb on + strand, within LRK55_RS09005 at 1973.242 kb on + strand, within LRK55_RS09005 at 1973.242 kb on + strand, within LRK55_RS09005 at 1973.243 kb on - strand, within LRK55_RS09005 at 1973.325 kb on + strand, within LRK55_RS09005 at 1973.326 kb on - strand, within LRK55_RS09005 at 1973.461 kb on + strand, within LRK55_RS09005 at 1973.461 kb on + strand, within LRK55_RS09005 at 1973.462 kb on - strand, within LRK55_RS09005 at 1973.462 kb on - strand, within LRK55_RS09005 at 1973.566 kb on + strand, within LRK55_RS09005 at 1973.567 kb on - strand, within LRK55_RS09005 at 1973.567 kb on - strand, within LRK55_RS09005 at 1973.588 kb on - strand, within LRK55_RS09005 at 1973.588 kb on - strand, within LRK55_RS09005 at 1973.649 kb on - strand, within LRK55_RS09005 at 1973.760 kb on + strand, within LRK55_RS09005 at 1973.761 kb on - strand, within LRK55_RS09005 at 1973.761 kb on - strand, within LRK55_RS09005 at 1973.847 kb on + strand, within LRK55_RS09005 at 1973.848 kb on - strand, within LRK55_RS09005 at 1973.848 kb on - strand, within LRK55_RS09005 at 1973.869 kb on + strand, within LRK55_RS09005 at 1974.064 kb on + strand, within LRK55_RS09005 at 1974.065 kb on - strand, within LRK55_RS09005 at 1974.092 kb on + strand, within LRK55_RS09005 at 1974.093 kb on - strand, within LRK55_RS09005 at 1974.112 kb on + strand, within LRK55_RS09005 at 1974.113 kb on - strand, within LRK55_RS09005 at 1974.113 kb on - strand, within LRK55_RS09005 at 1974.136 kb on + strand, within LRK55_RS09005 at 1974.136 kb on + strand, within LRK55_RS09005 at 1974.136 kb on + strand, within LRK55_RS09005 at 1974.137 kb on - strand, within LRK55_RS09005 at 1974.156 kb on - strand, within LRK55_RS09005 at 1974.156 kb on - strand, within LRK55_RS09005 at 1974.221 kb on - strand, within LRK55_RS09005 at 1974.221 kb on - strand, within LRK55_RS09005 at 1974.378 kb on + strand, within LRK55_RS09005 at 1974.433 kb on + strand, within LRK55_RS09005 at 1974.433 kb on + strand, within LRK55_RS09005 at 1974.508 kb on + strand, within LRK55_RS09005 at 1974.509 kb on - strand, within LRK55_RS09005 at 1974.597 kb on - strand, within LRK55_RS09005 at 1974.597 kb on - strand, within LRK55_RS09005 at 1974.795 kb on + strand at 1974.869 kb on + strand at 1974.869 kb on + strand at 1974.870 kb on - strand at 1974.870 kb on - strand at 1974.938 kb on + strand, within LRK55_RS09010 at 1974.974 kb on + strand, within LRK55_RS09010 at 1974.974 kb on + strand, within LRK55_RS09010 at 1974.974 kb on + strand, within LRK55_RS09010 at 1974.974 kb on + strand, within LRK55_RS09010 at 1974.974 kb on + strand, within LRK55_RS09010 at 1974.975 kb on - strand, within LRK55_RS09010 at 1974.975 kb on - strand, within LRK55_RS09010 at 1974.975 kb on - strand, within LRK55_RS09010 at 1974.975 kb on - strand, within LRK55_RS09010 at 1974.975 kb on - strand, within LRK55_RS09010 at 1974.975 kb on - strand, within LRK55_RS09010 at 1974.999 kb on - strand, within LRK55_RS09010 at 1975.294 kb on - strand, within LRK55_RS09010 at 1975.455 kb on + strand, within LRK55_RS09010 at 1975.456 kb on - strand, within LRK55_RS09010 at 1975.519 kb on + strand, within LRK55_RS09010 at 1975.519 kb on + strand, within LRK55_RS09010 at 1975.520 kb on - strand, within LRK55_RS09010
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 1,972,470 + +0.3 1,972,556 + LRK55_RS09000 0.33 -2.6 1,972,556 + LRK55_RS09000 0.33 +1.6 1,972,556 + LRK55_RS09000 0.33 -0.8 1,972,556 + LRK55_RS09000 0.33 +0.3 1,972,556 + LRK55_RS09000 0.33 -0.9 1,972,556 + LRK55_RS09000 0.33 -1.8 1,972,556 + LRK55_RS09000 0.33 +1.2 1,972,556 + LRK55_RS09000 0.33 +0.6 1,972,557 - LRK55_RS09000 0.34 -0.5 1,972,557 - LRK55_RS09000 0.34 +0.3 1,972,557 - LRK55_RS09000 0.34 -1.0 1,972,622 + LRK55_RS09000 0.59 -1.8 1,972,654 + LRK55_RS09000 0.71 -0.7 1,972,654 + LRK55_RS09000 0.71 +0.7 1,972,654 + LRK55_RS09000 0.71 +1.7 1,972,655 - LRK55_RS09000 0.71 +1.4 1,972,655 - LRK55_RS09000 0.71 -1.7 1,972,733 + -1.0 1,972,733 + +0.0 1,972,733 + +1.1 1,972,733 + +0.2 1,972,733 + +1.7 1,972,733 + -1.9 1,972,734 - -1.1 1,972,734 - +0.7 1,972,734 - +0.6 1,972,734 - -0.0 1,972,737 + -2.9 1,972,737 + +0.4 1,972,737 + -0.6 1,972,737 + -1.1 1,972,737 + -2.2 1,972,737 + -0.3 1,972,738 - +1.5 1,972,738 - +0.3 1,972,738 - +0.9 1,972,738 - +0.1 1,972,738 - +0.1 1,972,738 - -0.9 1,972,738 - +2.4 1,972,738 - +1.8 1,972,738 - +0.2 1,972,738 - +0.5 1,972,786 + -0.1 1,972,786 + -0.2 1,972,786 + -3.5 1,972,786 + -3.3 1,972,787 - +0.0 1,972,787 - +1.0 1,972,787 - -0.2 1,972,808 + -1.9 1,972,809 - -0.1 1,972,872 + -2.8 1,972,873 - +0.1 1,972,880 + +0.6 1,972,880 + -0.2 1,972,881 - -0.6 1,972,923 + -2.1 1,972,924 - -0.1 1,972,924 - +0.3 1,972,952 - -0.9 1,972,971 + +0.1 1,973,046 + -2.2 1,973,046 + -0.0 1,973,047 - -1.8 1,973,047 - -2.7 1,973,047 - -1.0 1,973,047 - +0.9 1,973,105 + -2.2 1,973,105 + -3.2 1,973,106 - +0.2 1,973,188 - LRK55_RS09005 0.11 +0.8 1,973,215 + LRK55_RS09005 0.12 +1.9 1,973,215 + LRK55_RS09005 0.12 -0.5 1,973,242 + LRK55_RS09005 0.14 -0.5 1,973,242 + LRK55_RS09005 0.14 -1.7 1,973,243 - LRK55_RS09005 0.14 -2.1 1,973,325 + LRK55_RS09005 0.18 -0.4 1,973,326 - LRK55_RS09005 0.18 -2.1 1,973,461 + LRK55_RS09005 0.26 -0.3 1,973,461 + LRK55_RS09005 0.26 -0.3 1,973,462 - LRK55_RS09005 0.26 +0.1 1,973,462 - LRK55_RS09005 0.26 +0.3 1,973,566 + LRK55_RS09005 0.32 -1.6 1,973,567 - LRK55_RS09005 0.32 -1.3 1,973,567 - LRK55_RS09005 0.32 +0.3 1,973,588 - LRK55_RS09005 0.33 -0.2 1,973,588 - LRK55_RS09005 0.33 +0.2 1,973,649 - LRK55_RS09005 0.36 -0.3 1,973,760 + LRK55_RS09005 0.42 -0.3 1,973,761 - LRK55_RS09005 0.43 +0.6 1,973,761 - LRK55_RS09005 0.43 -0.3 1,973,847 + LRK55_RS09005 0.47 -2.0 1,973,848 - LRK55_RS09005 0.47 -0.4 1,973,848 - LRK55_RS09005 0.47 -0.8 1,973,869 + LRK55_RS09005 0.49 -3.6 1,974,064 + LRK55_RS09005 0.59 +0.3 1,974,065 - LRK55_RS09005 0.59 -0.6 1,974,092 + LRK55_RS09005 0.61 +1.1 1,974,093 - LRK55_RS09005 0.61 +0.3 1,974,112 + LRK55_RS09005 0.62 -0.3 1,974,113 - LRK55_RS09005 0.62 +2.1 1,974,113 - LRK55_RS09005 0.62 -0.8 1,974,136 + LRK55_RS09005 0.63 -2.5 1,974,136 + LRK55_RS09005 0.63 -0.7 1,974,136 + LRK55_RS09005 0.63 -0.1 1,974,137 - LRK55_RS09005 0.63 +0.1 1,974,156 - LRK55_RS09005 0.65 +1.7 1,974,156 - LRK55_RS09005 0.65 +0.1 1,974,221 - LRK55_RS09005 0.68 -0.3 1,974,221 - LRK55_RS09005 0.68 +0.9 1,974,378 + LRK55_RS09005 0.77 +0.1 1,974,433 + LRK55_RS09005 0.80 +0.5 1,974,433 + LRK55_RS09005 0.80 -1.1 1,974,508 + LRK55_RS09005 0.84 +0.0 1,974,509 - LRK55_RS09005 0.84 -2.9 1,974,597 - LRK55_RS09005 0.89 +0.9 1,974,597 - LRK55_RS09005 0.89 -0.3 1,974,795 + -1.5 1,974,869 + +0.2 1,974,869 + -3.1 1,974,870 - -1.6 1,974,870 - +1.8 1,974,938 + LRK55_RS09010 0.16 +0.9 1,974,974 + LRK55_RS09010 0.19 +0.5 1,974,974 + LRK55_RS09010 0.19 +0.0 1,974,974 + LRK55_RS09010 0.19 +1.1 1,974,974 + LRK55_RS09010 0.19 -1.1 1,974,974 + LRK55_RS09010 0.19 -1.1 1,974,975 - LRK55_RS09010 0.19 -1.2 1,974,975 - LRK55_RS09010 0.19 -0.1 1,974,975 - LRK55_RS09010 0.19 -0.2 1,974,975 - LRK55_RS09010 0.19 +0.9 1,974,975 - LRK55_RS09010 0.19 +0.7 1,974,975 - LRK55_RS09010 0.19 +0.2 1,974,999 - LRK55_RS09010 0.22 -0.7 1,975,294 - LRK55_RS09010 0.52 +2.0 1,975,455 + LRK55_RS09010 0.68 -1.3 1,975,456 - LRK55_RS09010 0.68 +0.2 1,975,519 + LRK55_RS09010 0.74 -0.6 1,975,519 + LRK55_RS09010 0.74 +1.5 1,975,520 - LRK55_RS09010 0.74 -0.6
Or see this region's nucleotide sequence