Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS08355 and LRK55_RS08360 are separated by 113 nucleotides LRK55_RS08360 and LRK55_RS08365 overlap by 4 nucleotides LRK55_RS08365 and LRK55_RS08370 are separated by 112 nucleotides LRK55_RS08370 and LRK55_RS08375 are separated by 7 nucleotides
LRK55_RS08355: LRK55_RS08355 - hypothetical protein, at 1,828,232 to 1,828,489
_RS08355
LRK55_RS08360: LRK55_RS08360 - shikimate dehydrogenase, at 1,828,603 to 1,829,433
_RS08360
LRK55_RS08365: LRK55_RS08365 - YkgJ family cysteine cluster protein, at 1,829,430 to 1,829,696
_RS08365
LRK55_RS08370: LRK55_RS08370 - hypothetical protein, at 1,829,809 to 1,830,360
_RS08370
LRK55_RS08375: LRK55_RS08375 - gamma carbonic anhydrase family protein, at 1,830,368 to 1,830,901
_RS08375
Position (kb)
1829
1830 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1828.569 kb on + strand at 1828.569 kb on + strand at 1828.569 kb on + strand at 1828.569 kb on + strand at 1828.569 kb on + strand at 1828.569 kb on + strand at 1828.569 kb on + strand at 1828.569 kb on + strand at 1828.569 kb on + strand at 1828.569 kb on + strand at 1828.569 kb on + strand at 1828.569 kb on + strand at 1828.569 kb on + strand at 1828.569 kb on + strand at 1828.570 kb on - strand at 1828.570 kb on - strand at 1828.570 kb on - strand at 1828.570 kb on - strand at 1828.570 kb on - strand at 1828.570 kb on - strand at 1828.570 kb on - strand at 1828.570 kb on - strand at 1828.570 kb on - strand at 1828.705 kb on + strand, within LRK55_RS08360 at 1828.705 kb on + strand, within LRK55_RS08360 at 1828.705 kb on + strand, within LRK55_RS08360 at 1828.705 kb on + strand, within LRK55_RS08360 at 1828.705 kb on + strand, within LRK55_RS08360 at 1828.705 kb on + strand, within LRK55_RS08360 at 1828.706 kb on - strand, within LRK55_RS08360 at 1828.706 kb on - strand, within LRK55_RS08360 at 1828.706 kb on - strand, within LRK55_RS08360 at 1828.706 kb on - strand, within LRK55_RS08360 at 1828.706 kb on - strand, within LRK55_RS08360 at 1828.706 kb on - strand, within LRK55_RS08360 at 1829.128 kb on + strand, within LRK55_RS08360 at 1829.158 kb on + strand, within LRK55_RS08360 at 1829.158 kb on + strand, within LRK55_RS08360 at 1829.158 kb on + strand, within LRK55_RS08360 at 1829.158 kb on + strand, within LRK55_RS08360 at 1829.158 kb on + strand, within LRK55_RS08360 at 1829.158 kb on + strand, within LRK55_RS08360 at 1829.158 kb on + strand, within LRK55_RS08360 at 1829.159 kb on - strand, within LRK55_RS08360 at 1829.159 kb on - strand, within LRK55_RS08360 at 1829.159 kb on - strand, within LRK55_RS08360 at 1829.159 kb on - strand, within LRK55_RS08360 at 1829.159 kb on - strand, within LRK55_RS08360 at 1829.159 kb on - strand, within LRK55_RS08360 at 1829.248 kb on + strand, within LRK55_RS08360 at 1829.248 kb on + strand, within LRK55_RS08360 at 1829.249 kb on - strand, within LRK55_RS08360 at 1829.249 kb on - strand, within LRK55_RS08360 at 1829.249 kb on - strand, within LRK55_RS08360 at 1829.260 kb on + strand, within LRK55_RS08360 at 1829.260 kb on + strand, within LRK55_RS08360 at 1829.261 kb on - strand, within LRK55_RS08360 at 1829.261 kb on - strand, within LRK55_RS08360 at 1829.261 kb on - strand, within LRK55_RS08360 at 1829.261 kb on - strand, within LRK55_RS08360 at 1829.404 kb on + strand at 1829.404 kb on + strand at 1829.404 kb on + strand at 1829.404 kb on + strand at 1829.405 kb on - strand at 1829.405 kb on - strand at 1829.832 kb on - strand at 1829.899 kb on + strand, within LRK55_RS08370 at 1829.899 kb on + strand, within LRK55_RS08370 at 1829.899 kb on + strand, within LRK55_RS08370 at 1829.900 kb on - strand, within LRK55_RS08370 at 1829.900 kb on - strand, within LRK55_RS08370 at 1829.900 kb on - strand, within LRK55_RS08370 at 1829.900 kb on - strand, within LRK55_RS08370 at 1830.076 kb on + strand, within LRK55_RS08370 at 1830.076 kb on + strand, within LRK55_RS08370 at 1830.076 kb on + strand, within LRK55_RS08370 at 1830.077 kb on - strand, within LRK55_RS08370 at 1830.077 kb on - strand, within LRK55_RS08370 at 1830.384 kb on + strand at 1830.384 kb on + strand at 1830.384 kb on + strand at 1830.385 kb on - strand at 1830.385 kb on - strand at 1830.385 kb on - strand at 1830.405 kb on + strand at 1830.405 kb on + strand at 1830.405 kb on + strand at 1830.405 kb on + strand at 1830.405 kb on + strand at 1830.405 kb on + strand at 1830.405 kb on + strand at 1830.406 kb on - strand at 1830.406 kb on - strand at 1830.406 kb on - strand at 1830.406 kb on - strand at 1830.406 kb on - strand at 1830.406 kb on - strand at 1830.406 kb on - strand at 1830.420 kb on + strand at 1830.420 kb on + strand at 1830.420 kb on + strand at 1830.421 kb on - strand at 1830.421 kb on - strand at 1830.600 kb on + strand, within LRK55_RS08375 at 1830.600 kb on + strand, within LRK55_RS08375 at 1830.601 kb on - strand, within LRK55_RS08375 at 1830.601 kb on - strand, within LRK55_RS08375 at 1830.601 kb on - strand, within LRK55_RS08375 at 1830.601 kb on - strand, within LRK55_RS08375 at 1830.601 kb on - strand, within LRK55_RS08375 at 1830.684 kb on + strand, within LRK55_RS08375 at 1830.685 kb on - strand, within LRK55_RS08375
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 1,828,569 + -0.3 1,828,569 + -2.8 1,828,569 + +2.0 1,828,569 + -2.1 1,828,569 + +0.7 1,828,569 + +0.9 1,828,569 + -0.6 1,828,569 + -2.2 1,828,569 + -2.3 1,828,569 + +0.2 1,828,569 + -0.7 1,828,569 + -0.7 1,828,569 + -0.5 1,828,569 + -3.3 1,828,570 - -1.6 1,828,570 - +0.6 1,828,570 - +0.7 1,828,570 - -0.6 1,828,570 - -0.3 1,828,570 - -2.4 1,828,570 - -0.4 1,828,570 - -0.0 1,828,570 - -1.1 1,828,705 + LRK55_RS08360 0.12 -0.3 1,828,705 + LRK55_RS08360 0.12 +0.4 1,828,705 + LRK55_RS08360 0.12 +0.1 1,828,705 + LRK55_RS08360 0.12 -1.8 1,828,705 + LRK55_RS08360 0.12 -1.9 1,828,705 + LRK55_RS08360 0.12 -1.1 1,828,706 - LRK55_RS08360 0.12 -2.8 1,828,706 - LRK55_RS08360 0.12 -3.7 1,828,706 - LRK55_RS08360 0.12 -1.2 1,828,706 - LRK55_RS08360 0.12 -1.6 1,828,706 - LRK55_RS08360 0.12 -2.1 1,828,706 - LRK55_RS08360 0.12 -1.3 1,829,128 + LRK55_RS08360 0.63 -0.8 1,829,158 + LRK55_RS08360 0.67 -1.0 1,829,158 + LRK55_RS08360 0.67 -0.3 1,829,158 + LRK55_RS08360 0.67 +0.2 1,829,158 + LRK55_RS08360 0.67 -3.2 1,829,158 + LRK55_RS08360 0.67 -1.5 1,829,158 + LRK55_RS08360 0.67 -1.2 1,829,158 + LRK55_RS08360 0.67 -0.1 1,829,159 - LRK55_RS08360 0.67 +0.8 1,829,159 - LRK55_RS08360 0.67 -1.6 1,829,159 - LRK55_RS08360 0.67 -1.9 1,829,159 - LRK55_RS08360 0.67 -2.1 1,829,159 - LRK55_RS08360 0.67 -1.9 1,829,159 - LRK55_RS08360 0.67 -3.6 1,829,248 + LRK55_RS08360 0.78 -2.2 1,829,248 + LRK55_RS08360 0.78 -0.5 1,829,249 - LRK55_RS08360 0.78 -0.6 1,829,249 - LRK55_RS08360 0.78 -4.8 1,829,249 - LRK55_RS08360 0.78 +0.1 1,829,260 + LRK55_RS08360 0.79 -1.1 1,829,260 + LRK55_RS08360 0.79 -1.9 1,829,261 - LRK55_RS08360 0.79 -1.9 1,829,261 - LRK55_RS08360 0.79 +0.0 1,829,261 - LRK55_RS08360 0.79 +3.0 1,829,261 - LRK55_RS08360 0.79 +0.2 1,829,404 + -1.7 1,829,404 + -2.8 1,829,404 + +0.9 1,829,404 + -0.2 1,829,405 - -3.9 1,829,405 - -1.4 1,829,832 - -0.1 1,829,899 + LRK55_RS08370 0.16 -0.2 1,829,899 + LRK55_RS08370 0.16 +1.9 1,829,899 + LRK55_RS08370 0.16 -0.3 1,829,900 - LRK55_RS08370 0.16 -0.6 1,829,900 - LRK55_RS08370 0.16 +0.1 1,829,900 - LRK55_RS08370 0.16 -0.9 1,829,900 - LRK55_RS08370 0.16 +1.7 1,830,076 + LRK55_RS08370 0.48 -1.6 1,830,076 + LRK55_RS08370 0.48 -1.6 1,830,076 + LRK55_RS08370 0.48 -1.4 1,830,077 - LRK55_RS08370 0.49 +0.6 1,830,077 - LRK55_RS08370 0.49 +0.5 1,830,384 + -0.9 1,830,384 + -0.5 1,830,384 + -1.5 1,830,385 - +2.2 1,830,385 - -0.7 1,830,385 - -3.0 1,830,405 + -0.2 1,830,405 + +0.4 1,830,405 + -1.1 1,830,405 + +1.8 1,830,405 + -1.6 1,830,405 + +2.2 1,830,405 + +0.3 1,830,406 - -0.4 1,830,406 - -1.3 1,830,406 - -0.8 1,830,406 - -0.3 1,830,406 - +0.2 1,830,406 - +0.5 1,830,406 - +0.3 1,830,420 + -0.4 1,830,420 + +0.2 1,830,420 + +1.3 1,830,421 - +0.2 1,830,421 - +0.4 1,830,600 + LRK55_RS08375 0.43 -0.4 1,830,600 + LRK55_RS08375 0.43 -0.6 1,830,601 - LRK55_RS08375 0.44 -1.1 1,830,601 - LRK55_RS08375 0.44 -0.1 1,830,601 - LRK55_RS08375 0.44 +0.2 1,830,601 - LRK55_RS08375 0.44 +0.3 1,830,601 - LRK55_RS08375 0.44 -0.8 1,830,684 + LRK55_RS08375 0.59 +0.1 1,830,685 - LRK55_RS08375 0.59 +0.3
Or see this region's nucleotide sequence