Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS03590 and LRK55_RS03595 are separated by 3 nucleotides LRK55_RS03595 and LRK55_RS03600 are separated by 237 nucleotides LRK55_RS03600 and LRK55_RS03605 are separated by 124 nucleotides LRK55_RS03605 and LRK55_RS03610 are separated by 61 nucleotides
LRK55_RS03590: LRK55_RS03590 - hypothetical protein, at 756,638 to 757,018
_RS03590
LRK55_RS03595: LRK55_RS03595 - DUF3106 domain-containing protein, at 757,022 to 757,549
_RS03595
LRK55_RS03600: LRK55_RS03600 - NAD(P) transhydrogenase subunit alpha, at 757,787 to 758,890
_RS03600
LRK55_RS03605: LRK55_RS03605 - cytidine deaminase, at 759,015 to 759,431
_RS03605
LRK55_RS03610: LRK55_RS03610 - nitroreductase, at 759,493 to 760,062
_RS03610
Position (kb) 757
758
759 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 756.957 kb on - strand, within LRK55_RS03590 at 756.974 kb on + strand, within LRK55_RS03590 at 756.975 kb on - strand, within LRK55_RS03590 at 757.456 kb on + strand, within LRK55_RS03595 at 757.457 kb on - strand, within LRK55_RS03595 at 757.591 kb on + strand at 757.592 kb on - strand at 757.592 kb on - strand at 757.673 kb on + strand at 757.673 kb on + strand at 757.673 kb on + strand at 757.673 kb on + strand at 757.673 kb on + strand at 757.673 kb on + strand at 757.673 kb on + strand at 757.674 kb on - strand at 757.788 kb on + strand at 757.788 kb on + strand at 757.788 kb on + strand at 757.788 kb on + strand at 757.788 kb on + strand at 757.789 kb on - strand at 757.789 kb on - strand at 757.789 kb on - strand at 757.789 kb on - strand at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.917 kb on + strand, within LRK55_RS03600 at 757.918 kb on - strand, within LRK55_RS03600 at 757.918 kb on - strand, within LRK55_RS03600 at 757.918 kb on - strand, within LRK55_RS03600 at 757.918 kb on - strand, within LRK55_RS03600 at 757.918 kb on - strand, within LRK55_RS03600 at 758.307 kb on + strand, within LRK55_RS03600 at 758.307 kb on + strand, within LRK55_RS03600 at 758.308 kb on - strand, within LRK55_RS03600 at 758.308 kb on - strand, within LRK55_RS03600 at 758.601 kb on + strand, within LRK55_RS03600 at 758.736 kb on + strand, within LRK55_RS03600 at 758.736 kb on + strand, within LRK55_RS03600 at 758.736 kb on + strand, within LRK55_RS03600 at 758.736 kb on + strand, within LRK55_RS03600 at 758.736 kb on + strand, within LRK55_RS03600 at 758.736 kb on + strand, within LRK55_RS03600 at 758.736 kb on + strand, within LRK55_RS03600 at 758.737 kb on - strand, within LRK55_RS03600 at 758.737 kb on - strand, within LRK55_RS03600 at 758.737 kb on - strand, within LRK55_RS03600 at 758.787 kb on - strand at 758.787 kb on - strand at 758.877 kb on - strand at 758.923 kb on + strand at 758.923 kb on + strand at 758.923 kb on + strand at 758.965 kb on + strand at 758.965 kb on + strand at 758.965 kb on + strand at 758.966 kb on - strand at 758.966 kb on - strand at 758.966 kb on - strand at 758.966 kb on - strand at 759.082 kb on - strand, within LRK55_RS03605 at 759.082 kb on - strand, within LRK55_RS03605 at 759.082 kb on - strand, within LRK55_RS03605 at 759.091 kb on - strand, within LRK55_RS03605 at 759.171 kb on + strand, within LRK55_RS03605 at 759.171 kb on + strand, within LRK55_RS03605 at 759.171 kb on + strand, within LRK55_RS03605 at 759.171 kb on + strand, within LRK55_RS03605 at 759.172 kb on - strand, within LRK55_RS03605 at 759.172 kb on - strand, within LRK55_RS03605 at 759.754 kb on + strand, within LRK55_RS03610 at 759.754 kb on + strand, within LRK55_RS03610 at 759.754 kb on + strand, within LRK55_RS03610 at 759.755 kb on - strand, within LRK55_RS03610 at 759.755 kb on - strand, within LRK55_RS03610 at 759.755 kb on - strand, within LRK55_RS03610 at 759.875 kb on - strand, within LRK55_RS03610 at 759.875 kb on - strand, within LRK55_RS03610
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 756,957 - LRK55_RS03590 0.84 -2.3 756,974 + LRK55_RS03590 0.88 +1.0 756,975 - LRK55_RS03590 0.88 -1.0 757,456 + LRK55_RS03595 0.82 -2.6 757,457 - LRK55_RS03595 0.82 +0.0 757,591 + +0.1 757,592 - -1.3 757,592 - +0.0 757,673 + +0.0 757,673 + -1.2 757,673 + +0.9 757,673 + +0.8 757,673 + +0.5 757,673 + +1.6 757,673 + +1.9 757,674 - +0.1 757,788 + -0.5 757,788 + -0.5 757,788 + +0.4 757,788 + -0.0 757,788 + +0.2 757,789 - +0.0 757,789 - -3.2 757,789 - -2.2 757,789 - +0.0 757,917 + LRK55_RS03600 0.12 -0.6 757,917 + LRK55_RS03600 0.12 +1.4 757,917 + LRK55_RS03600 0.12 -1.7 757,917 + LRK55_RS03600 0.12 +0.7 757,917 + LRK55_RS03600 0.12 +1.1 757,917 + LRK55_RS03600 0.12 +1.4 757,917 + LRK55_RS03600 0.12 +0.3 757,917 + LRK55_RS03600 0.12 -2.0 757,917 + LRK55_RS03600 0.12 +0.0 757,918 - LRK55_RS03600 0.12 +0.0 757,918 - LRK55_RS03600 0.12 -0.7 757,918 - LRK55_RS03600 0.12 -2.7 757,918 - LRK55_RS03600 0.12 +0.6 757,918 - LRK55_RS03600 0.12 +1.5 758,307 + LRK55_RS03600 0.47 +0.0 758,307 + LRK55_RS03600 0.47 +0.1 758,308 - LRK55_RS03600 0.47 -0.6 758,308 - LRK55_RS03600 0.47 +0.2 758,601 + LRK55_RS03600 0.74 -0.2 758,736 + LRK55_RS03600 0.86 -1.3 758,736 + LRK55_RS03600 0.86 +0.1 758,736 + LRK55_RS03600 0.86 -1.8 758,736 + LRK55_RS03600 0.86 +0.8 758,736 + LRK55_RS03600 0.86 -0.4 758,736 + LRK55_RS03600 0.86 +0.5 758,736 + LRK55_RS03600 0.86 -0.2 758,737 - LRK55_RS03600 0.86 +0.0 758,737 - LRK55_RS03600 0.86 -0.4 758,737 - LRK55_RS03600 0.86 -0.5 758,787 - +0.0 758,787 - +1.2 758,877 - -0.3 758,923 + -1.3 758,923 + -3.1 758,923 + +0.4 758,965 + -0.3 758,965 + +0.8 758,965 + -1.3 758,966 - +1.1 758,966 - -0.6 758,966 - +1.0 758,966 - +3.6 759,082 - LRK55_RS03605 0.16 +2.3 759,082 - LRK55_RS03605 0.16 +0.4 759,082 - LRK55_RS03605 0.16 +0.4 759,091 - LRK55_RS03605 0.18 +0.8 759,171 + LRK55_RS03605 0.37 -0.7 759,171 + LRK55_RS03605 0.37 +0.5 759,171 + LRK55_RS03605 0.37 -1.5 759,171 + LRK55_RS03605 0.37 +1.0 759,172 - LRK55_RS03605 0.38 -0.2 759,172 - LRK55_RS03605 0.38 +1.1 759,754 + LRK55_RS03610 0.46 +0.5 759,754 + LRK55_RS03610 0.46 -1.2 759,754 + LRK55_RS03610 0.46 +0.8 759,755 - LRK55_RS03610 0.46 -0.0 759,755 - LRK55_RS03610 0.46 -1.0 759,755 - LRK55_RS03610 0.46 +1.2 759,875 - LRK55_RS03610 0.67 +0.6 759,875 - LRK55_RS03610 0.67 -1.4
Or see this region's nucleotide sequence