Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS03575 and LRK55_RS03580 overlap by 1 nucleotides LRK55_RS03580 and LRK55_RS03585 are separated by 150 nucleotides LRK55_RS03585 and LRK55_RS03590 overlap by 4 nucleotides LRK55_RS03590 and LRK55_RS03595 are separated by 3 nucleotides
LRK55_RS03575: LRK55_RS03575 - NAD(P)(+) transhydrogenase (Re/Si-specific) subunit beta, at 754,144 to 755,601
_RS03575
LRK55_RS03580: LRK55_RS03580 - NAD(P) transhydrogenase subunit alpha, at 755,601 to 755,906
_RS03580
LRK55_RS03585: LRK55_RS03585 - RNA polymerase sigma factor, at 756,057 to 756,641
_RS03585
LRK55_RS03590: LRK55_RS03590 - hypothetical protein, at 756,638 to 757,018
_RS03590
LRK55_RS03595: LRK55_RS03595 - DUF3106 domain-containing protein, at 757,022 to 757,549
_RS03595
Position (kb)
756
757 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 755.232 kb on + strand, within LRK55_RS03575 at 755.271 kb on - strand, within LRK55_RS03575 at 755.271 kb on - strand, within LRK55_RS03575 at 755.327 kb on + strand, within LRK55_RS03575 at 755.327 kb on + strand, within LRK55_RS03575 at 755.327 kb on + strand, within LRK55_RS03575 at 755.327 kb on + strand, within LRK55_RS03575 at 755.328 kb on - strand, within LRK55_RS03575 at 755.328 kb on - strand, within LRK55_RS03575 at 755.328 kb on - strand, within LRK55_RS03575 at 755.480 kb on + strand at 755.481 kb on - strand at 755.567 kb on + strand at 755.567 kb on + strand at 755.567 kb on + strand at 755.567 kb on + strand at 755.568 kb on - strand at 755.672 kb on - strand, within LRK55_RS03580 at 755.851 kb on + strand, within LRK55_RS03580 at 755.859 kb on - strand, within LRK55_RS03580 at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.881 kb on + strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.882 kb on - strand at 755.956 kb on + strand at 755.956 kb on + strand at 755.956 kb on + strand at 755.956 kb on + strand at 755.956 kb on + strand at 755.957 kb on - strand at 755.957 kb on - strand at 755.957 kb on - strand at 755.957 kb on - strand at 755.957 kb on - strand at 755.957 kb on - strand at 756.243 kb on + strand, within LRK55_RS03585 at 756.243 kb on + strand, within LRK55_RS03585 at 756.244 kb on - strand, within LRK55_RS03585 at 756.453 kb on + strand, within LRK55_RS03585 at 756.453 kb on + strand, within LRK55_RS03585 at 756.454 kb on - strand, within LRK55_RS03585 at 756.957 kb on - strand, within LRK55_RS03590 at 756.974 kb on + strand, within LRK55_RS03590 at 756.975 kb on - strand, within LRK55_RS03590 at 757.456 kb on + strand, within LRK55_RS03595 at 757.457 kb on - strand, within LRK55_RS03595 at 757.591 kb on + strand at 757.592 kb on - strand at 757.592 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 755,232 + LRK55_RS03575 0.75 +0.4 755,271 - LRK55_RS03575 0.77 -0.4 755,271 - LRK55_RS03575 0.77 -2.5 755,327 + LRK55_RS03575 0.81 -0.8 755,327 + LRK55_RS03575 0.81 -0.6 755,327 + LRK55_RS03575 0.81 -1.0 755,327 + LRK55_RS03575 0.81 -0.9 755,328 - LRK55_RS03575 0.81 -0.0 755,328 - LRK55_RS03575 0.81 -0.8 755,328 - LRK55_RS03575 0.81 +0.4 755,480 + +2.0 755,481 - +1.1 755,567 + +0.0 755,567 + -0.4 755,567 + -1.2 755,567 + -0.3 755,568 - +0.3 755,672 - LRK55_RS03580 0.23 +0.7 755,851 + LRK55_RS03580 0.82 +1.0 755,859 - LRK55_RS03580 0.84 -2.9 755,881 + +0.4 755,881 + +1.8 755,881 + -0.5 755,881 + +1.2 755,881 + +1.0 755,881 + +0.3 755,881 + -0.8 755,881 + +0.0 755,881 + +0.2 755,881 + -3.2 755,881 + +1.0 755,881 + +0.3 755,881 + -0.0 755,882 - +0.7 755,882 - -0.0 755,882 - +1.0 755,882 - +0.2 755,882 - -0.7 755,882 - +0.2 755,882 - +0.5 755,882 - -2.8 755,882 - -2.6 755,882 - -2.2 755,882 - -1.2 755,882 - +0.2 755,882 - +0.1 755,882 - -0.2 755,956 + +0.9 755,956 + +0.5 755,956 + +0.7 755,956 + -0.3 755,956 + +0.2 755,957 - +0.8 755,957 - +0.7 755,957 - -0.6 755,957 - -0.6 755,957 - -0.8 755,957 - -0.5 756,243 + LRK55_RS03585 0.32 +1.1 756,243 + LRK55_RS03585 0.32 -0.3 756,244 - LRK55_RS03585 0.32 +0.1 756,453 + LRK55_RS03585 0.68 +1.0 756,453 + LRK55_RS03585 0.68 -0.2 756,454 - LRK55_RS03585 0.68 +1.5 756,957 - LRK55_RS03590 0.84 -2.3 756,974 + LRK55_RS03590 0.88 +1.0 756,975 - LRK55_RS03590 0.88 -1.0 757,456 + LRK55_RS03595 0.82 -2.6 757,457 - LRK55_RS03595 0.82 +0.0 757,591 + +0.1 757,592 - -1.3 757,592 - +0.0
Or see this region's nucleotide sequence