Experiment: Outgrowth from agar plate co-culture with Inquilinus sp. 005-A5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00156 and MPMX20_00157 are separated by 3 nucleotides MPMX20_00157 and MPMX20_00158 are separated by 4 nucleotides MPMX20_00158 and MPMX20_00159 are separated by 180 nucleotides
MPMX20_00156: MPMX20_00156 - DNA-binding transcriptional repressor YiaJ, at 159,794 to 160,612
_00156
MPMX20_00157: MPMX20_00157 - Iron-sulfur protein, at 160,616 to 161,071
_00157
MPMX20_00158: MPMX20_00158 - Valine--pyruvate aminotransferase, at 161,076 to 162,332
_00158
MPMX20_00159: MPMX20_00159 - Periplasmic alpha-amylase, at 162,513 to 164,543
_00159
Position (kb) 161
162
163 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 160.092 kb on - strand, within MPMX20_00156 at 160.100 kb on - strand, within MPMX20_00156 at 160.107 kb on - strand, within MPMX20_00156 at 160.119 kb on + strand, within MPMX20_00156 at 160.130 kb on + strand, within MPMX20_00156 at 160.165 kb on - strand, within MPMX20_00156 at 160.186 kb on + strand, within MPMX20_00156 at 160.194 kb on + strand, within MPMX20_00156 at 160.194 kb on - strand, within MPMX20_00156 at 160.194 kb on - strand, within MPMX20_00156 at 160.231 kb on - strand, within MPMX20_00156 at 160.245 kb on - strand, within MPMX20_00156 at 160.279 kb on - strand, within MPMX20_00156 at 160.293 kb on - strand, within MPMX20_00156 at 160.314 kb on + strand, within MPMX20_00156 at 160.362 kb on + strand, within MPMX20_00156 at 160.407 kb on - strand, within MPMX20_00156 at 160.407 kb on - strand, within MPMX20_00156 at 160.446 kb on - strand, within MPMX20_00156 at 160.466 kb on + strand, within MPMX20_00156 at 160.543 kb on + strand at 160.590 kb on + strand at 160.590 kb on + strand at 160.598 kb on - strand at 160.598 kb on - strand at 160.598 kb on - strand at 160.665 kb on + strand, within MPMX20_00157 at 160.688 kb on - strand, within MPMX20_00157 at 160.736 kb on - strand, within MPMX20_00157 at 160.756 kb on + strand, within MPMX20_00157 at 160.763 kb on + strand, within MPMX20_00157 at 160.771 kb on - strand, within MPMX20_00157 at 160.823 kb on + strand, within MPMX20_00157 at 160.975 kb on - strand, within MPMX20_00157 at 160.987 kb on - strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.989 kb on + strand, within MPMX20_00157 at 160.997 kb on - strand, within MPMX20_00157 at 160.997 kb on - strand, within MPMX20_00157 at 161.020 kb on - strand, within MPMX20_00157 at 161.030 kb on - strand at 161.030 kb on - strand at 161.030 kb on - strand at 161.030 kb on - strand at 161.092 kb on + strand at 161.171 kb on - strand at 161.191 kb on + strand at 161.202 kb on + strand, within MPMX20_00158 at 161.210 kb on - strand, within MPMX20_00158 at 161.210 kb on - strand, within MPMX20_00158 at 161.220 kb on - strand, within MPMX20_00158 at 161.411 kb on + strand, within MPMX20_00158 at 161.449 kb on - strand, within MPMX20_00158 at 161.492 kb on + strand, within MPMX20_00158 at 161.500 kb on - strand, within MPMX20_00158 at 161.594 kb on + strand, within MPMX20_00158 at 161.594 kb on + strand, within MPMX20_00158 at 161.595 kb on + strand, within MPMX20_00158 at 161.668 kb on + strand, within MPMX20_00158 at 161.668 kb on + strand, within MPMX20_00158 at 161.668 kb on + strand, within MPMX20_00158 at 161.668 kb on + strand, within MPMX20_00158 at 161.676 kb on - strand, within MPMX20_00158 at 161.676 kb on - strand, within MPMX20_00158 at 161.676 kb on - strand, within MPMX20_00158 at 161.743 kb on + strand, within MPMX20_00158 at 161.784 kb on + strand, within MPMX20_00158 at 161.792 kb on - strand, within MPMX20_00158 at 161.792 kb on - strand, within MPMX20_00158 at 161.792 kb on - strand, within MPMX20_00158 at 161.816 kb on + strand, within MPMX20_00158 at 161.843 kb on + strand, within MPMX20_00158 at 161.865 kb on - strand, within MPMX20_00158 at 161.866 kb on - strand, within MPMX20_00158 at 161.888 kb on - strand, within MPMX20_00158 at 161.919 kb on + strand, within MPMX20_00158 at 161.970 kb on + strand, within MPMX20_00158 at 162.060 kb on - strand, within MPMX20_00158 at 162.065 kb on - strand, within MPMX20_00158 at 162.074 kb on + strand, within MPMX20_00158 at 162.082 kb on - strand, within MPMX20_00158 at 162.087 kb on - strand, within MPMX20_00158 at 162.103 kb on + strand, within MPMX20_00158 at 162.103 kb on + strand, within MPMX20_00158 at 162.106 kb on - strand, within MPMX20_00158 at 162.239 kb on + strand at 162.259 kb on + strand at 162.267 kb on - strand at 162.282 kb on + strand at 162.282 kb on + strand at 162.286 kb on - strand at 162.327 kb on + strand at 162.337 kb on + strand at 162.371 kb on + strand at 162.371 kb on + strand at 162.379 kb on - strand at 162.385 kb on - strand at 162.385 kb on - strand at 162.385 kb on - strand at 162.385 kb on - strand at 162.392 kb on + strand at 162.487 kb on + strand at 162.496 kb on - strand at 162.526 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.543 kb on - strand at 162.543 kb on - strand at 162.543 kb on - strand at 162.543 kb on - strand at 162.550 kb on + strand at 162.570 kb on + strand at 162.596 kb on + strand at 162.631 kb on + strand at 162.642 kb on + strand at 162.667 kb on + strand at 162.667 kb on + strand at 162.667 kb on + strand at 162.697 kb on - strand at 162.699 kb on + strand at 162.699 kb on + strand at 162.699 kb on + strand at 162.699 kb on + strand at 162.707 kb on - strand at 162.707 kb on - strand at 162.707 kb on - strand at 162.720 kb on + strand, within MPMX20_00159 at 162.728 kb on + strand, within MPMX20_00159 at 162.733 kb on + strand, within MPMX20_00159 at 162.736 kb on - strand, within MPMX20_00159 at 162.736 kb on - strand, within MPMX20_00159 at 162.743 kb on + strand, within MPMX20_00159 at 162.802 kb on - strand, within MPMX20_00159 at 162.807 kb on - strand, within MPMX20_00159 at 162.817 kb on - strand, within MPMX20_00159 at 162.837 kb on + strand, within MPMX20_00159 at 162.865 kb on - strand, within MPMX20_00159 at 162.875 kb on - strand, within MPMX20_00159 at 162.875 kb on - strand, within MPMX20_00159 at 162.917 kb on + strand, within MPMX20_00159 at 162.934 kb on - strand, within MPMX20_00159 at 162.983 kb on + strand, within MPMX20_00159 at 162.983 kb on + strand, within MPMX20_00159 at 163.055 kb on + strand, within MPMX20_00159 at 163.167 kb on - strand, within MPMX20_00159 at 163.191 kb on + strand, within MPMX20_00159 at 163.201 kb on - strand, within MPMX20_00159 at 163.231 kb on - strand, within MPMX20_00159 at 163.270 kb on + strand, within MPMX20_00159 at 163.271 kb on - strand, within MPMX20_00159 at 163.271 kb on - strand, within MPMX20_00159 at 163.278 kb on - strand, within MPMX20_00159 at 163.278 kb on - strand, within MPMX20_00159 at 163.285 kb on + strand, within MPMX20_00159
Per-strain Table
Position Strand Gene LocusTag Fraction Outgrowth from agar plate co-culture with Inquilinus sp. 005-A5 remove 160,092 - MPMX20_00156 0.36 +1.5 160,100 - MPMX20_00156 0.37 +2.0 160,107 - MPMX20_00156 0.38 +0.2 160,119 + MPMX20_00156 0.40 -0.4 160,130 + MPMX20_00156 0.41 -0.8 160,165 - MPMX20_00156 0.45 -1.6 160,186 + MPMX20_00156 0.48 +1.0 160,194 + MPMX20_00156 0.49 +0.5 160,194 - MPMX20_00156 0.49 -0.6 160,194 - MPMX20_00156 0.49 -1.4 160,231 - MPMX20_00156 0.53 -0.5 160,245 - MPMX20_00156 0.55 -0.7 160,279 - MPMX20_00156 0.59 -0.0 160,293 - MPMX20_00156 0.61 -1.2 160,314 + MPMX20_00156 0.63 -1.2 160,362 + MPMX20_00156 0.69 +0.6 160,407 - MPMX20_00156 0.75 -1.0 160,407 - MPMX20_00156 0.75 -0.8 160,446 - MPMX20_00156 0.80 -3.5 160,466 + MPMX20_00156 0.82 -1.0 160,543 + -0.6 160,590 + +0.3 160,590 + -0.0 160,598 - -1.2 160,598 - -0.8 160,598 - -0.3 160,665 + MPMX20_00157 0.11 -0.2 160,688 - MPMX20_00157 0.16 -0.2 160,736 - MPMX20_00157 0.26 -2.2 160,756 + MPMX20_00157 0.31 -0.3 160,763 + MPMX20_00157 0.32 +0.1 160,771 - MPMX20_00157 0.34 +0.5 160,823 + MPMX20_00157 0.45 -0.1 160,975 - MPMX20_00157 0.79 -0.2 160,987 - MPMX20_00157 0.81 -0.1 160,989 + MPMX20_00157 0.82 -0.1 160,989 + MPMX20_00157 0.82 -0.1 160,989 + MPMX20_00157 0.82 -1.7 160,989 + MPMX20_00157 0.82 +2.0 160,989 + MPMX20_00157 0.82 -0.4 160,997 - MPMX20_00157 0.84 -0.7 160,997 - MPMX20_00157 0.84 -0.9 161,020 - MPMX20_00157 0.89 -1.2 161,030 - -0.1 161,030 - +0.5 161,030 - +0.5 161,030 - +0.4 161,092 + +0.5 161,171 - +1.4 161,191 + -1.5 161,202 + MPMX20_00158 0.10 -0.1 161,210 - MPMX20_00158 0.11 +0.3 161,210 - MPMX20_00158 0.11 -0.9 161,220 - MPMX20_00158 0.11 -0.1 161,411 + MPMX20_00158 0.27 +0.3 161,449 - MPMX20_00158 0.30 +0.0 161,492 + MPMX20_00158 0.33 -2.1 161,500 - MPMX20_00158 0.34 +0.1 161,594 + MPMX20_00158 0.41 +0.7 161,594 + MPMX20_00158 0.41 +0.5 161,595 + MPMX20_00158 0.41 -1.2 161,668 + MPMX20_00158 0.47 +0.4 161,668 + MPMX20_00158 0.47 +0.6 161,668 + MPMX20_00158 0.47 -0.3 161,668 + MPMX20_00158 0.47 -0.3 161,676 - MPMX20_00158 0.48 -0.1 161,676 - MPMX20_00158 0.48 -0.8 161,676 - MPMX20_00158 0.48 +0.5 161,743 + MPMX20_00158 0.53 +1.1 161,784 + MPMX20_00158 0.56 -0.3 161,792 - MPMX20_00158 0.57 -0.0 161,792 - MPMX20_00158 0.57 +1.0 161,792 - MPMX20_00158 0.57 +0.1 161,816 + MPMX20_00158 0.59 -0.0 161,843 + MPMX20_00158 0.61 +0.0 161,865 - MPMX20_00158 0.63 -1.0 161,866 - MPMX20_00158 0.63 -0.5 161,888 - MPMX20_00158 0.65 -0.2 161,919 + MPMX20_00158 0.67 +1.4 161,970 + MPMX20_00158 0.71 -1.9 162,060 - MPMX20_00158 0.78 +1.7 162,065 - MPMX20_00158 0.79 -0.1 162,074 + MPMX20_00158 0.79 -0.4 162,082 - MPMX20_00158 0.80 +0.1 162,087 - MPMX20_00158 0.80 -0.5 162,103 + MPMX20_00158 0.82 +0.4 162,103 + MPMX20_00158 0.82 +0.3 162,106 - MPMX20_00158 0.82 +0.3 162,239 + +3.0 162,259 + +0.4 162,267 - -0.6 162,282 + +0.5 162,282 + -0.3 162,286 - -0.0 162,327 + -1.7 162,337 + -2.2 162,371 + +1.4 162,371 + +2.3 162,379 - -0.3 162,385 - -0.1 162,385 - -0.4 162,385 - -0.2 162,385 - -1.2 162,392 + -1.2 162,487 + -0.1 162,496 - +0.2 162,526 + -0.7 162,535 + -0.4 162,535 + +0.6 162,535 + -0.1 162,535 + +0.7 162,535 + -1.7 162,535 + -0.2 162,535 + -0.1 162,543 - -2.3 162,543 - -2.6 162,543 - +0.1 162,543 - -0.5 162,550 + -0.7 162,570 + -1.0 162,596 + -0.9 162,631 + -1.6 162,642 + -1.5 162,667 + -0.2 162,667 + +0.0 162,667 + -0.5 162,697 - +0.6 162,699 + +0.1 162,699 + +0.8 162,699 + +0.5 162,699 + +0.4 162,707 - +0.6 162,707 - -0.4 162,707 - +0.6 162,720 + MPMX20_00159 0.10 +3.0 162,728 + MPMX20_00159 0.11 +3.0 162,733 + MPMX20_00159 0.11 -3.6 162,736 - MPMX20_00159 0.11 +0.6 162,736 - MPMX20_00159 0.11 -1.1 162,743 + MPMX20_00159 0.11 +0.4 162,802 - MPMX20_00159 0.14 +0.0 162,807 - MPMX20_00159 0.14 +0.1 162,817 - MPMX20_00159 0.15 -2.0 162,837 + MPMX20_00159 0.16 -0.3 162,865 - MPMX20_00159 0.17 +0.2 162,875 - MPMX20_00159 0.18 +1.1 162,875 - MPMX20_00159 0.18 +0.2 162,917 + MPMX20_00159 0.20 +0.8 162,934 - MPMX20_00159 0.21 -0.1 162,983 + MPMX20_00159 0.23 +0.3 162,983 + MPMX20_00159 0.23 -1.0 163,055 + MPMX20_00159 0.27 -0.1 163,167 - MPMX20_00159 0.32 -1.0 163,191 + MPMX20_00159 0.33 -0.7 163,201 - MPMX20_00159 0.34 -0.1 163,231 - MPMX20_00159 0.35 -0.2 163,270 + MPMX20_00159 0.37 +0.6 163,271 - MPMX20_00159 0.37 -0.2 163,271 - MPMX20_00159 0.37 +1.3 163,278 - MPMX20_00159 0.38 +0.0 163,278 - MPMX20_00159 0.38 +0.2 163,285 + MPMX20_00159 0.38 +0.2
Or see this region's nucleotide sequence