Experiment: R2A_PIPES with Cadmium chloride 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS16340 and LRK55_RS16345 are separated by 10 nucleotides LRK55_RS16345 and LRK55_RS16350 overlap by 4 nucleotides LRK55_RS16350 and LRK55_RS16355 are separated by 10 nucleotides LRK55_RS16355 and LRK55_RS16360 overlap by 4 nucleotides
LRK55_RS16340: LRK55_RS16340 - phosphopyruvate hydratase, at 3,562,624 to 3,563,922
_RS16340
LRK55_RS16345: LRK55_RS16345 - cell division protein FtsB, at 3,563,933 to 3,564,268
_RS16345
LRK55_RS16350: LRK55_RS16350 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 3,564,265 to 3,564,963
_RS16350
LRK55_RS16355: LRK55_RS16355 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 3,564,974 to 3,565,447
_RS16355
LRK55_RS16360: LRK55_RS16360 - magnesium transporter, at 3,565,444 to 3,566,838
_RS16360
Position (kb)
3564
3565 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3563.701 kb on + strand, within LRK55_RS16340 at 3563.827 kb on + strand at 3563.827 kb on + strand at 3563.875 kb on + strand at 3563.875 kb on + strand at 3563.875 kb on + strand at 3563.875 kb on + strand at 3565.454 kb on + strand at 3565.454 kb on + strand at 3565.454 kb on + strand at 3565.454 kb on + strand at 3565.454 kb on + strand at 3565.454 kb on + strand at 3565.454 kb on + strand at 3565.454 kb on + strand at 3565.455 kb on - strand at 3565.455 kb on - strand at 3565.455 kb on - strand at 3565.455 kb on - strand at 3565.455 kb on - strand at 3565.695 kb on - strand, within LRK55_RS16360 at 3565.695 kb on - strand, within LRK55_RS16360 at 3565.695 kb on - strand, within LRK55_RS16360 at 3565.695 kb on - strand, within LRK55_RS16360 at 3565.880 kb on - strand, within LRK55_RS16360
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 64 uM remove 3,563,701 + LRK55_RS16340 0.83 -3.0 3,563,827 + -1.6 3,563,827 + -1.6 3,563,875 + -0.4 3,563,875 + -1.6 3,563,875 + -0.4 3,563,875 + -1.4 3,565,454 + +0.2 3,565,454 + +0.2 3,565,454 + -2.1 3,565,454 + -0.0 3,565,454 + +0.4 3,565,454 + -1.3 3,565,454 + +0.1 3,565,454 + +0.1 3,565,455 - +1.4 3,565,455 - -0.6 3,565,455 - -3.1 3,565,455 - +0.2 3,565,455 - -1.5 3,565,695 - LRK55_RS16360 0.18 -2.6 3,565,695 - LRK55_RS16360 0.18 -2.0 3,565,695 - LRK55_RS16360 0.18 -1.9 3,565,695 - LRK55_RS16360 0.18 -2.8 3,565,880 - LRK55_RS16360 0.31 +0.2
Or see this region's nucleotide sequence