Experiment: R2A_PIPES with Cadmium chloride 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS12365 and LRK55_RS12370 are separated by 85 nucleotides LRK55_RS12370 and LRK55_RS12375 overlap by 4 nucleotides
LRK55_RS12365: LRK55_RS12365 - bifunctional aspartate kinase/diaminopimelate decarboxylase, at 2,747,273 to 2,749,834
_RS12365
LRK55_RS12370: LRK55_RS12370 - endonuclease domain-containing protein, at 2,749,920 to 2,750,324
_RS12370
LRK55_RS12375: LRK55_RS12375 - UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L- glutamate epimerase, at 2,750,321 to 2,751,661
_RS12375
Position (kb)
2749
2750
2751 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2749.010 kb on + strand, within LRK55_RS12365 at 2749.010 kb on + strand, within LRK55_RS12365 at 2749.010 kb on + strand, within LRK55_RS12365 at 2749.010 kb on + strand, within LRK55_RS12365 at 2749.010 kb on + strand, within LRK55_RS12365 at 2749.011 kb on - strand, within LRK55_RS12365 at 2749.011 kb on - strand, within LRK55_RS12365 at 2749.011 kb on - strand, within LRK55_RS12365 at 2749.011 kb on - strand, within LRK55_RS12365 at 2749.011 kb on - strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.301 kb on + strand, within LRK55_RS12365 at 2749.302 kb on - strand, within LRK55_RS12365 at 2749.302 kb on - strand, within LRK55_RS12365 at 2749.302 kb on - strand, within LRK55_RS12365 at 2749.302 kb on - strand, within LRK55_RS12365 at 2749.302 kb on - strand, within LRK55_RS12365 at 2749.302 kb on - strand, within LRK55_RS12365 at 2749.302 kb on - strand, within LRK55_RS12365 at 2749.448 kb on + strand, within LRK55_RS12365 at 2749.457 kb on + strand, within LRK55_RS12365 at 2749.458 kb on - strand, within LRK55_RS12365 at 2749.484 kb on + strand, within LRK55_RS12365 at 2749.601 kb on + strand at 2749.601 kb on + strand at 2749.601 kb on + strand at 2749.601 kb on + strand at 2749.601 kb on + strand at 2749.601 kb on + strand at 2749.602 kb on - strand at 2749.661 kb on + strand at 2749.661 kb on + strand at 2749.662 kb on - strand at 2749.662 kb on - strand at 2749.662 kb on - strand at 2749.662 kb on - strand at 2749.769 kb on + strand at 2749.770 kb on - strand at 2749.793 kb on + strand at 2749.793 kb on + strand at 2749.793 kb on + strand at 2749.793 kb on + strand at 2749.793 kb on + strand at 2749.793 kb on + strand at 2749.794 kb on - strand at 2749.794 kb on - strand at 2749.794 kb on - strand at 2749.794 kb on - strand at 2749.794 kb on - strand at 2750.055 kb on + strand, within LRK55_RS12370 at 2750.055 kb on + strand, within LRK55_RS12370 at 2750.055 kb on + strand, within LRK55_RS12370 at 2750.055 kb on + strand, within LRK55_RS12370 at 2750.055 kb on + strand, within LRK55_RS12370 at 2750.055 kb on + strand, within LRK55_RS12370 at 2750.055 kb on + strand, within LRK55_RS12370 at 2750.056 kb on - strand, within LRK55_RS12370 at 2750.056 kb on - strand, within LRK55_RS12370 at 2750.056 kb on - strand, within LRK55_RS12370 at 2750.056 kb on - strand, within LRK55_RS12370 at 2750.056 kb on - strand, within LRK55_RS12370 at 2750.056 kb on - strand, within LRK55_RS12370 at 2750.056 kb on - strand, within LRK55_RS12370 at 2750.154 kb on + strand, within LRK55_RS12370 at 2750.154 kb on + strand, within LRK55_RS12370 at 2750.155 kb on - strand, within LRK55_RS12370 at 2750.155 kb on - strand, within LRK55_RS12370 at 2750.155 kb on - strand, within LRK55_RS12370
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 64 uM remove 2,749,010 + LRK55_RS12365 0.68 +0.7 2,749,010 + LRK55_RS12365 0.68 +0.3 2,749,010 + LRK55_RS12365 0.68 -0.3 2,749,010 + LRK55_RS12365 0.68 -3.2 2,749,010 + LRK55_RS12365 0.68 -0.7 2,749,011 - LRK55_RS12365 0.68 -1.7 2,749,011 - LRK55_RS12365 0.68 -1.8 2,749,011 - LRK55_RS12365 0.68 -3.6 2,749,011 - LRK55_RS12365 0.68 -2.2 2,749,011 - LRK55_RS12365 0.68 -3.8 2,749,301 + LRK55_RS12365 0.79 -0.9 2,749,301 + LRK55_RS12365 0.79 -1.4 2,749,301 + LRK55_RS12365 0.79 -0.7 2,749,301 + LRK55_RS12365 0.79 -0.0 2,749,301 + LRK55_RS12365 0.79 -2.8 2,749,301 + LRK55_RS12365 0.79 -2.6 2,749,301 + LRK55_RS12365 0.79 -0.6 2,749,301 + LRK55_RS12365 0.79 -1.5 2,749,301 + LRK55_RS12365 0.79 -2.4 2,749,301 + LRK55_RS12365 0.79 -1.5 2,749,301 + LRK55_RS12365 0.79 -2.2 2,749,301 + LRK55_RS12365 0.79 -2.8 2,749,301 + LRK55_RS12365 0.79 +0.2 2,749,301 + LRK55_RS12365 0.79 -0.7 2,749,302 - LRK55_RS12365 0.79 -3.1 2,749,302 - LRK55_RS12365 0.79 -0.6 2,749,302 - LRK55_RS12365 0.79 -2.0 2,749,302 - LRK55_RS12365 0.79 -2.5 2,749,302 - LRK55_RS12365 0.79 -1.7 2,749,302 - LRK55_RS12365 0.79 +1.3 2,749,302 - LRK55_RS12365 0.79 -1.9 2,749,448 + LRK55_RS12365 0.85 -2.0 2,749,457 + LRK55_RS12365 0.85 -2.2 2,749,458 - LRK55_RS12365 0.85 -1.3 2,749,484 + LRK55_RS12365 0.86 +0.1 2,749,601 + +1.1 2,749,601 + +0.2 2,749,601 + +0.0 2,749,601 + -0.6 2,749,601 + +0.5 2,749,601 + -0.7 2,749,602 - +0.3 2,749,661 + -0.3 2,749,661 + -0.8 2,749,662 - -1.1 2,749,662 - -1.1 2,749,662 - -2.9 2,749,662 - -1.0 2,749,769 + -1.2 2,749,770 - +0.3 2,749,793 + +0.5 2,749,793 + +0.5 2,749,793 + -1.3 2,749,793 + +0.5 2,749,793 + +1.1 2,749,793 + +1.1 2,749,794 - +0.6 2,749,794 - -1.3 2,749,794 - +0.5 2,749,794 - +0.6 2,749,794 - -0.3 2,750,055 + LRK55_RS12370 0.33 -2.1 2,750,055 + LRK55_RS12370 0.33 +2.0 2,750,055 + LRK55_RS12370 0.33 -0.1 2,750,055 + LRK55_RS12370 0.33 -0.8 2,750,055 + LRK55_RS12370 0.33 +0.9 2,750,055 + LRK55_RS12370 0.33 -1.8 2,750,055 + LRK55_RS12370 0.33 -0.5 2,750,056 - LRK55_RS12370 0.34 +1.0 2,750,056 - LRK55_RS12370 0.34 +1.0 2,750,056 - LRK55_RS12370 0.34 +1.1 2,750,056 - LRK55_RS12370 0.34 +1.8 2,750,056 - LRK55_RS12370 0.34 +0.8 2,750,056 - LRK55_RS12370 0.34 -1.2 2,750,056 - LRK55_RS12370 0.34 +1.3 2,750,154 + LRK55_RS12370 0.58 -0.5 2,750,154 + LRK55_RS12370 0.58 -2.2 2,750,155 - LRK55_RS12370 0.58 -0.7 2,750,155 - LRK55_RS12370 0.58 +2.0 2,750,155 - LRK55_RS12370 0.58 +3.6
Or see this region's nucleotide sequence