Experiment: R2A_PIPES with Cadmium chloride 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS10280 and LRK55_RS10285 are separated by 27 nucleotides LRK55_RS10285 and LRK55_RS10290 are separated by 41 nucleotides LRK55_RS10290 and LRK55_RS10295 are separated by 263 nucleotides
LRK55_RS10280: LRK55_RS10280 - SCP2 sterol-binding domain-containing protein, at 2,266,087 to 2,266,737
_RS10280
LRK55_RS10285: LRK55_RS10285 - tRNA (cytidine(34)-2'-O)-methyltransferase, at 2,266,765 to 2,267,235
_RS10285
LRK55_RS10290: LRK55_RS10290 - hypothetical protein, at 2,267,277 to 2,267,480
_RS10290
LRK55_RS10295: LRK55_RS10295 - pitrilysin family protein, at 2,267,744 to 2,270,599
_RS10295
Position (kb)
2267
2268 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2266.294 kb on + strand, within LRK55_RS10280 at 2266.567 kb on + strand, within LRK55_RS10280 at 2266.567 kb on + strand, within LRK55_RS10280 at 2266.568 kb on - strand, within LRK55_RS10280 at 2266.568 kb on - strand, within LRK55_RS10280 at 2266.749 kb on - strand at 2266.811 kb on + strand at 2266.811 kb on + strand at 2266.812 kb on - strand, within LRK55_RS10285 at 2267.075 kb on + strand, within LRK55_RS10285 at 2267.075 kb on + strand, within LRK55_RS10285 at 2267.075 kb on + strand, within LRK55_RS10285 at 2267.076 kb on - strand, within LRK55_RS10285 at 2267.084 kb on + strand, within LRK55_RS10285 at 2267.084 kb on + strand, within LRK55_RS10285 at 2267.084 kb on + strand, within LRK55_RS10285 at 2267.084 kb on + strand, within LRK55_RS10285 at 2267.084 kb on + strand, within LRK55_RS10285 at 2267.085 kb on - strand, within LRK55_RS10285 at 2267.085 kb on - strand, within LRK55_RS10285 at 2267.085 kb on - strand, within LRK55_RS10285 at 2267.085 kb on - strand, within LRK55_RS10285 at 2267.465 kb on - strand at 2267.564 kb on + strand at 2267.564 kb on + strand at 2267.565 kb on - strand at 2267.575 kb on + strand at 2267.583 kb on - strand at 2267.705 kb on - strand at 2267.719 kb on + strand at 2267.719 kb on + strand at 2267.719 kb on + strand at 2267.719 kb on + strand at 2267.719 kb on + strand at 2267.719 kb on + strand at 2267.720 kb on - strand at 2267.720 kb on - strand at 2267.720 kb on - strand at 2267.720 kb on - strand at 2267.873 kb on + strand at 2267.873 kb on + strand at 2267.873 kb on + strand at 2267.873 kb on + strand at 2267.874 kb on - strand at 2267.875 kb on + strand at 2267.875 kb on + strand at 2267.957 kb on + strand at 2267.957 kb on + strand at 2267.957 kb on + strand at 2267.958 kb on - strand at 2267.958 kb on - strand at 2267.958 kb on - strand at 2267.975 kb on + strand at 2267.975 kb on + strand at 2267.976 kb on - strand at 2267.976 kb on - strand at 2268.125 kb on + strand, within LRK55_RS10295 at 2268.125 kb on + strand, within LRK55_RS10295 at 2268.125 kb on + strand, within LRK55_RS10295 at 2268.126 kb on - strand, within LRK55_RS10295 at 2268.126 kb on - strand, within LRK55_RS10295 at 2268.126 kb on - strand, within LRK55_RS10295 at 2268.278 kb on + strand, within LRK55_RS10295 at 2268.333 kb on - strand, within LRK55_RS10295 at 2268.333 kb on - strand, within LRK55_RS10295 at 2268.333 kb on - strand, within LRK55_RS10295 at 2268.410 kb on + strand, within LRK55_RS10295 at 2268.410 kb on + strand, within LRK55_RS10295 at 2268.410 kb on + strand, within LRK55_RS10295 at 2268.410 kb on + strand, within LRK55_RS10295 at 2268.410 kb on + strand, within LRK55_RS10295 at 2268.410 kb on + strand, within LRK55_RS10295 at 2268.410 kb on + strand, within LRK55_RS10295 at 2268.413 kb on + strand, within LRK55_RS10295 at 2268.414 kb on - strand, within LRK55_RS10295
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 64 uM remove 2,266,294 + LRK55_RS10280 0.32 -2.0 2,266,567 + LRK55_RS10280 0.74 -0.6 2,266,567 + LRK55_RS10280 0.74 -2.2 2,266,568 - LRK55_RS10280 0.74 -2.5 2,266,568 - LRK55_RS10280 0.74 +0.8 2,266,749 - +3.4 2,266,811 + -0.9 2,266,811 + -1.8 2,266,812 - LRK55_RS10285 0.10 +1.1 2,267,075 + LRK55_RS10285 0.66 -0.8 2,267,075 + LRK55_RS10285 0.66 -0.0 2,267,075 + LRK55_RS10285 0.66 +1.6 2,267,076 - LRK55_RS10285 0.66 -2.4 2,267,084 + LRK55_RS10285 0.68 -0.5 2,267,084 + LRK55_RS10285 0.68 +1.6 2,267,084 + LRK55_RS10285 0.68 +1.2 2,267,084 + LRK55_RS10285 0.68 +0.1 2,267,084 + LRK55_RS10285 0.68 +1.3 2,267,085 - LRK55_RS10285 0.68 +0.1 2,267,085 - LRK55_RS10285 0.68 -0.1 2,267,085 - LRK55_RS10285 0.68 +1.4 2,267,085 - LRK55_RS10285 0.68 +1.5 2,267,465 - -0.7 2,267,564 + -0.3 2,267,564 + +0.2 2,267,565 - +0.0 2,267,575 + -0.9 2,267,583 - -0.0 2,267,705 - -0.4 2,267,719 + +0.4 2,267,719 + -0.1 2,267,719 + +0.5 2,267,719 + +0.3 2,267,719 + +0.1 2,267,719 + +0.2 2,267,720 - -0.7 2,267,720 - +1.3 2,267,720 - -0.6 2,267,720 - +2.8 2,267,873 + -1.0 2,267,873 + -0.2 2,267,873 + -0.5 2,267,873 + -0.3 2,267,874 - -0.7 2,267,875 + -0.9 2,267,875 + -0.6 2,267,957 + +0.2 2,267,957 + -0.5 2,267,957 + -0.6 2,267,958 - -2.9 2,267,958 - -1.3 2,267,958 - -2.5 2,267,975 + +0.2 2,267,975 + -3.4 2,267,976 - -2.8 2,267,976 - -0.6 2,268,125 + LRK55_RS10295 0.13 -0.4 2,268,125 + LRK55_RS10295 0.13 -0.8 2,268,125 + LRK55_RS10295 0.13 -0.2 2,268,126 - LRK55_RS10295 0.13 -1.7 2,268,126 - LRK55_RS10295 0.13 -0.8 2,268,126 - LRK55_RS10295 0.13 -0.6 2,268,278 + LRK55_RS10295 0.19 -0.7 2,268,333 - LRK55_RS10295 0.21 -0.3 2,268,333 - LRK55_RS10295 0.21 -1.6 2,268,333 - LRK55_RS10295 0.21 -2.3 2,268,410 + LRK55_RS10295 0.23 -1.8 2,268,410 + LRK55_RS10295 0.23 -2.2 2,268,410 + LRK55_RS10295 0.23 -1.5 2,268,410 + LRK55_RS10295 0.23 -0.8 2,268,410 + LRK55_RS10295 0.23 -0.7 2,268,410 + LRK55_RS10295 0.23 -1.2 2,268,410 + LRK55_RS10295 0.23 +0.5 2,268,413 + LRK55_RS10295 0.23 -1.5 2,268,414 - LRK55_RS10295 0.23 -1.0
Or see this region's nucleotide sequence