Experiment: R2A_PIPES with Cadmium chloride 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS06560 and LRK55_RS06565 are separated by 162 nucleotides LRK55_RS06565 and LRK55_RS06570 are separated by 67 nucleotides LRK55_RS06570 and LRK55_RS06575 are separated by 150 nucleotides LRK55_RS06575 and LRK55_RS06580 are separated by 27 nucleotides
LRK55_RS06560: LRK55_RS06560 - zinc-ribbon and DUF3426 domain-containing protein, at 1,418,377 to 1,419,234
_RS06560
LRK55_RS06565: LRK55_RS06565 - helix-turn-helix domain-containing protein, at 1,419,397 to 1,419,714
_RS06565
LRK55_RS06570: LRK55_RS06570 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 1,419,782 to 1,421,392
_RS06570
LRK55_RS06575: LRK55_RS06575 - glutathione S-transferase, at 1,421,543 to 1,422,253
_RS06575
LRK55_RS06580: LRK55_RS06580 - DUF2782 domain-containing protein, at 1,422,281 to 1,422,817
_RS06580
Position (kb)
1419
1420
1421
1422 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1419.232 kb on + strand at 1419.248 kb on - strand at 1419.248 kb on - strand at 1419.248 kb on - strand at 1419.248 kb on - strand at 1419.251 kb on + strand at 1419.251 kb on + strand at 1419.252 kb on - strand at 1419.315 kb on - strand at 1419.342 kb on + strand at 1419.342 kb on + strand at 1419.527 kb on - strand, within LRK55_RS06565 at 1419.712 kb on + strand at 1419.712 kb on + strand at 1419.712 kb on + strand at 1419.713 kb on - strand at 1419.713 kb on - strand at 1419.713 kb on - strand at 1419.713 kb on - strand at 1419.713 kb on - strand at 1419.746 kb on + strand at 1419.746 kb on + strand at 1419.746 kb on + strand at 1419.747 kb on - strand at 1419.748 kb on + strand at 1419.748 kb on + strand at 1419.748 kb on + strand at 1419.748 kb on + strand at 1419.748 kb on + strand at 1419.748 kb on + strand at 1419.749 kb on - strand at 1419.749 kb on - strand at 1419.749 kb on - strand at 1419.749 kb on - strand at 1420.244 kb on + strand, within LRK55_RS06570 at 1420.245 kb on - strand, within LRK55_RS06570 at 1420.340 kb on + strand, within LRK55_RS06570 at 1420.362 kb on - strand, within LRK55_RS06570 at 1420.456 kb on - strand, within LRK55_RS06570 at 1420.456 kb on - strand, within LRK55_RS06570 at 1420.500 kb on - strand, within LRK55_RS06570 at 1420.697 kb on + strand, within LRK55_RS06570 at 1420.697 kb on + strand, within LRK55_RS06570 at 1420.697 kb on + strand, within LRK55_RS06570 at 1420.697 kb on + strand, within LRK55_RS06570 at 1420.829 kb on + strand, within LRK55_RS06570 at 1421.156 kb on + strand, within LRK55_RS06570 at 1421.392 kb on - strand at 1421.393 kb on + strand at 1421.394 kb on - strand at 1421.486 kb on + strand at 1421.486 kb on + strand at 1421.486 kb on + strand at 1421.486 kb on + strand at 1421.486 kb on + strand at 1421.486 kb on + strand at 1421.486 kb on + strand at 1421.487 kb on - strand at 1421.487 kb on - strand at 1421.487 kb on - strand at 1421.487 kb on - strand at 1421.487 kb on - strand at 1421.497 kb on + strand at 1421.498 kb on - strand at 1421.498 kb on - strand at 1421.498 kb on - strand at 1421.498 kb on - strand at 1421.498 kb on - strand at 1421.549 kb on + strand at 1421.558 kb on + strand at 1421.558 kb on + strand at 1421.558 kb on + strand at 1421.558 kb on + strand at 1421.558 kb on + strand at 1421.558 kb on + strand at 1421.558 kb on + strand at 1421.559 kb on - strand at 1421.559 kb on - strand at 1421.559 kb on - strand at 1421.559 kb on - strand at 1421.561 kb on + strand at 1421.562 kb on - strand at 1421.562 kb on - strand at 1421.562 kb on - strand at 1421.562 kb on - strand at 1421.627 kb on + strand, within LRK55_RS06575 at 1421.627 kb on + strand, within LRK55_RS06575 at 1421.627 kb on + strand, within LRK55_RS06575 at 1421.627 kb on + strand, within LRK55_RS06575 at 1421.627 kb on + strand, within LRK55_RS06575 at 1421.627 kb on + strand, within LRK55_RS06575 at 1421.627 kb on + strand, within LRK55_RS06575 at 1421.627 kb on + strand, within LRK55_RS06575 at 1421.628 kb on - strand, within LRK55_RS06575 at 1421.628 kb on - strand, within LRK55_RS06575 at 1421.628 kb on - strand, within LRK55_RS06575 at 1421.628 kb on - strand, within LRK55_RS06575 at 1421.673 kb on - strand, within LRK55_RS06575 at 1421.768 kb on + strand, within LRK55_RS06575 at 1421.768 kb on + strand, within LRK55_RS06575 at 1421.769 kb on - strand, within LRK55_RS06575 at 1421.769 kb on - strand, within LRK55_RS06575 at 1421.769 kb on - strand, within LRK55_RS06575 at 1421.769 kb on - strand, within LRK55_RS06575 at 1421.769 kb on - strand, within LRK55_RS06575 at 1421.769 kb on - strand, within LRK55_RS06575 at 1421.769 kb on - strand, within LRK55_RS06575 at 1421.769 kb on - strand, within LRK55_RS06575 at 1421.819 kb on + strand, within LRK55_RS06575 at 1421.819 kb on + strand, within LRK55_RS06575 at 1421.820 kb on - strand, within LRK55_RS06575 at 1421.820 kb on - strand, within LRK55_RS06575 at 1421.820 kb on - strand, within LRK55_RS06575 at 1421.820 kb on - strand, within LRK55_RS06575 at 1421.900 kb on + strand, within LRK55_RS06575 at 1421.900 kb on + strand, within LRK55_RS06575 at 1421.900 kb on + strand, within LRK55_RS06575 at 1421.900 kb on + strand, within LRK55_RS06575 at 1421.900 kb on + strand, within LRK55_RS06575 at 1421.900 kb on + strand, within LRK55_RS06575 at 1421.900 kb on + strand, within LRK55_RS06575 at 1421.901 kb on - strand, within LRK55_RS06575 at 1421.901 kb on - strand, within LRK55_RS06575 at 1421.901 kb on - strand, within LRK55_RS06575 at 1421.903 kb on + strand, within LRK55_RS06575 at 1421.903 kb on + strand, within LRK55_RS06575 at 1421.903 kb on + strand, within LRK55_RS06575 at 1421.903 kb on + strand, within LRK55_RS06575 at 1421.904 kb on - strand, within LRK55_RS06575 at 1421.904 kb on - strand, within LRK55_RS06575 at 1421.904 kb on - strand, within LRK55_RS06575 at 1421.969 kb on + strand, within LRK55_RS06575 at 1421.969 kb on + strand, within LRK55_RS06575 at 1421.970 kb on - strand, within LRK55_RS06575 at 1421.970 kb on - strand, within LRK55_RS06575 at 1421.970 kb on - strand, within LRK55_RS06575 at 1421.970 kb on - strand, within LRK55_RS06575 at 1421.970 kb on - strand, within LRK55_RS06575 at 1421.970 kb on - strand, within LRK55_RS06575 at 1421.978 kb on + strand, within LRK55_RS06575 at 1421.978 kb on + strand, within LRK55_RS06575 at 1421.979 kb on - strand, within LRK55_RS06575 at 1421.979 kb on - strand, within LRK55_RS06575 at 1422.035 kb on + strand, within LRK55_RS06575 at 1422.035 kb on + strand, within LRK55_RS06575 at 1422.035 kb on + strand, within LRK55_RS06575 at 1422.035 kb on + strand, within LRK55_RS06575 at 1422.035 kb on + strand, within LRK55_RS06575 at 1422.035 kb on + strand, within LRK55_RS06575 at 1422.036 kb on - strand, within LRK55_RS06575 at 1422.036 kb on - strand, within LRK55_RS06575 at 1422.036 kb on - strand, within LRK55_RS06575 at 1422.036 kb on - strand, within LRK55_RS06575 at 1422.036 kb on - strand, within LRK55_RS06575 at 1422.036 kb on - strand, within LRK55_RS06575 at 1422.077 kb on + strand, within LRK55_RS06575 at 1422.077 kb on + strand, within LRK55_RS06575 at 1422.077 kb on + strand, within LRK55_RS06575 at 1422.077 kb on + strand, within LRK55_RS06575 at 1422.077 kb on + strand, within LRK55_RS06575 at 1422.077 kb on + strand, within LRK55_RS06575 at 1422.077 kb on + strand, within LRK55_RS06575 at 1422.077 kb on + strand, within LRK55_RS06575 at 1422.078 kb on - strand, within LRK55_RS06575 at 1422.078 kb on - strand, within LRK55_RS06575 at 1422.078 kb on - strand, within LRK55_RS06575 at 1422.078 kb on - strand, within LRK55_RS06575 at 1422.078 kb on - strand, within LRK55_RS06575 at 1422.078 kb on - strand, within LRK55_RS06575 at 1422.078 kb on - strand, within LRK55_RS06575 at 1422.078 kb on - strand, within LRK55_RS06575 at 1422.078 kb on - strand, within LRK55_RS06575 at 1422.083 kb on + strand, within LRK55_RS06575 at 1422.084 kb on - strand, within LRK55_RS06575 at 1422.173 kb on + strand, within LRK55_RS06575 at 1422.173 kb on + strand, within LRK55_RS06575 at 1422.174 kb on - strand, within LRK55_RS06575 at 1422.227 kb on + strand at 1422.228 kb on - strand at 1422.251 kb on + strand at 1422.252 kb on - strand at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.360 kb on + strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.361 kb on - strand, within LRK55_RS06580 at 1422.375 kb on - strand, within LRK55_RS06580
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 64 uM remove 1,419,232 + -1.8 1,419,248 - -0.6 1,419,248 - -2.3 1,419,248 - -0.6 1,419,248 - -2.8 1,419,251 + +1.1 1,419,251 + +0.9 1,419,252 - +0.4 1,419,315 - -0.8 1,419,342 + +2.8 1,419,342 + +2.2 1,419,527 - LRK55_RS06565 0.41 -2.1 1,419,712 + -0.4 1,419,712 + +0.9 1,419,712 + +0.3 1,419,713 - +1.3 1,419,713 - +2.4 1,419,713 - -0.5 1,419,713 - -0.8 1,419,713 - +0.9 1,419,746 + -2.2 1,419,746 + +1.1 1,419,746 + -0.6 1,419,747 - +1.6 1,419,748 + -1.0 1,419,748 + -2.8 1,419,748 + -0.2 1,419,748 + -1.3 1,419,748 + +1.2 1,419,748 + -0.2 1,419,749 - +0.7 1,419,749 - -0.3 1,419,749 - -1.3 1,419,749 - -0.9 1,420,244 + LRK55_RS06570 0.29 +0.8 1,420,245 - LRK55_RS06570 0.29 +1.9 1,420,340 + LRK55_RS06570 0.35 -1.1 1,420,362 - LRK55_RS06570 0.36 -1.1 1,420,456 - LRK55_RS06570 0.42 +0.2 1,420,456 - LRK55_RS06570 0.42 -1.3 1,420,500 - LRK55_RS06570 0.45 +0.2 1,420,697 + LRK55_RS06570 0.57 -2.1 1,420,697 + LRK55_RS06570 0.57 -1.9 1,420,697 + LRK55_RS06570 0.57 +1.2 1,420,697 + LRK55_RS06570 0.57 -1.1 1,420,829 + LRK55_RS06570 0.65 -0.3 1,421,156 + LRK55_RS06570 0.85 -1.1 1,421,392 - +0.4 1,421,393 + -3.1 1,421,394 - -0.5 1,421,486 + +0.3 1,421,486 + +0.5 1,421,486 + +0.3 1,421,486 + -1.5 1,421,486 + +0.6 1,421,486 + -0.5 1,421,486 + -0.9 1,421,487 - -0.5 1,421,487 - +0.8 1,421,487 - -1.1 1,421,487 - -0.9 1,421,487 - +2.6 1,421,497 + -0.8 1,421,498 - +0.4 1,421,498 - +0.5 1,421,498 - -0.5 1,421,498 - -1.7 1,421,498 - +0.2 1,421,549 + -0.8 1,421,558 + +0.0 1,421,558 + +1.2 1,421,558 + +0.6 1,421,558 + +0.0 1,421,558 + -2.1 1,421,558 + +0.3 1,421,558 + +0.6 1,421,559 - -0.2 1,421,559 - -0.9 1,421,559 - +1.7 1,421,559 - +0.9 1,421,561 + -0.4 1,421,562 - +1.5 1,421,562 - -1.0 1,421,562 - +0.8 1,421,562 - -2.5 1,421,627 + LRK55_RS06575 0.12 -2.1 1,421,627 + LRK55_RS06575 0.12 -0.1 1,421,627 + LRK55_RS06575 0.12 -0.2 1,421,627 + LRK55_RS06575 0.12 -1.8 1,421,627 + LRK55_RS06575 0.12 -1.3 1,421,627 + LRK55_RS06575 0.12 -0.7 1,421,627 + LRK55_RS06575 0.12 -0.3 1,421,627 + LRK55_RS06575 0.12 -0.3 1,421,628 - LRK55_RS06575 0.12 +0.3 1,421,628 - LRK55_RS06575 0.12 -0.3 1,421,628 - LRK55_RS06575 0.12 +0.8 1,421,628 - LRK55_RS06575 0.12 +1.3 1,421,673 - LRK55_RS06575 0.18 -0.6 1,421,768 + LRK55_RS06575 0.32 -0.3 1,421,768 + LRK55_RS06575 0.32 +0.7 1,421,769 - LRK55_RS06575 0.32 -0.6 1,421,769 - LRK55_RS06575 0.32 -0.3 1,421,769 - LRK55_RS06575 0.32 -0.3 1,421,769 - LRK55_RS06575 0.32 -2.5 1,421,769 - LRK55_RS06575 0.32 +0.1 1,421,769 - LRK55_RS06575 0.32 -0.8 1,421,769 - LRK55_RS06575 0.32 +0.1 1,421,769 - LRK55_RS06575 0.32 -0.6 1,421,819 + LRK55_RS06575 0.39 -0.2 1,421,819 + LRK55_RS06575 0.39 +1.6 1,421,820 - LRK55_RS06575 0.39 +0.2 1,421,820 - LRK55_RS06575 0.39 +0.1 1,421,820 - LRK55_RS06575 0.39 -0.8 1,421,820 - LRK55_RS06575 0.39 +1.8 1,421,900 + LRK55_RS06575 0.50 +0.6 1,421,900 + LRK55_RS06575 0.50 -2.3 1,421,900 + LRK55_RS06575 0.50 -3.4 1,421,900 + LRK55_RS06575 0.50 +0.1 1,421,900 + LRK55_RS06575 0.50 -1.3 1,421,900 + LRK55_RS06575 0.50 -1.3 1,421,900 + LRK55_RS06575 0.50 -2.2 1,421,901 - LRK55_RS06575 0.50 +1.5 1,421,901 - LRK55_RS06575 0.50 +0.2 1,421,901 - LRK55_RS06575 0.50 -1.1 1,421,903 + LRK55_RS06575 0.51 +0.8 1,421,903 + LRK55_RS06575 0.51 -1.0 1,421,903 + LRK55_RS06575 0.51 +0.7 1,421,903 + LRK55_RS06575 0.51 +0.5 1,421,904 - LRK55_RS06575 0.51 +0.5 1,421,904 - LRK55_RS06575 0.51 -2.5 1,421,904 - LRK55_RS06575 0.51 -0.8 1,421,969 + LRK55_RS06575 0.60 -0.6 1,421,969 + LRK55_RS06575 0.60 +2.2 1,421,970 - LRK55_RS06575 0.60 +0.6 1,421,970 - LRK55_RS06575 0.60 -3.6 1,421,970 - LRK55_RS06575 0.60 +1.0 1,421,970 - LRK55_RS06575 0.60 +1.7 1,421,970 - LRK55_RS06575 0.60 -1.6 1,421,970 - LRK55_RS06575 0.60 -0.1 1,421,978 + LRK55_RS06575 0.61 +1.2 1,421,978 + LRK55_RS06575 0.61 -1.3 1,421,979 - LRK55_RS06575 0.61 -1.1 1,421,979 - LRK55_RS06575 0.61 +1.7 1,422,035 + LRK55_RS06575 0.69 +0.3 1,422,035 + LRK55_RS06575 0.69 +1.2 1,422,035 + LRK55_RS06575 0.69 +0.3 1,422,035 + LRK55_RS06575 0.69 +0.2 1,422,035 + LRK55_RS06575 0.69 -1.6 1,422,035 + LRK55_RS06575 0.69 +0.4 1,422,036 - LRK55_RS06575 0.69 +0.7 1,422,036 - LRK55_RS06575 0.69 +1.5 1,422,036 - LRK55_RS06575 0.69 -1.4 1,422,036 - LRK55_RS06575 0.69 -1.9 1,422,036 - LRK55_RS06575 0.69 +0.2 1,422,036 - LRK55_RS06575 0.69 +0.2 1,422,077 + LRK55_RS06575 0.75 +1.2 1,422,077 + LRK55_RS06575 0.75 -0.3 1,422,077 + LRK55_RS06575 0.75 +1.0 1,422,077 + LRK55_RS06575 0.75 -2.7 1,422,077 + LRK55_RS06575 0.75 +0.2 1,422,077 + LRK55_RS06575 0.75 +0.7 1,422,077 + LRK55_RS06575 0.75 -1.6 1,422,077 + LRK55_RS06575 0.75 +0.1 1,422,078 - LRK55_RS06575 0.75 -0.7 1,422,078 - LRK55_RS06575 0.75 -1.2 1,422,078 - LRK55_RS06575 0.75 +0.2 1,422,078 - LRK55_RS06575 0.75 +0.5 1,422,078 - LRK55_RS06575 0.75 +1.3 1,422,078 - LRK55_RS06575 0.75 -2.7 1,422,078 - LRK55_RS06575 0.75 +2.2 1,422,078 - LRK55_RS06575 0.75 -2.5 1,422,078 - LRK55_RS06575 0.75 +0.1 1,422,083 + LRK55_RS06575 0.76 +0.3 1,422,084 - LRK55_RS06575 0.76 -4.1 1,422,173 + LRK55_RS06575 0.89 -1.4 1,422,173 + LRK55_RS06575 0.89 -0.9 1,422,174 - LRK55_RS06575 0.89 +2.8 1,422,227 + +1.5 1,422,228 - -2.1 1,422,251 + +0.7 1,422,252 - -0.3 1,422,360 + LRK55_RS06580 0.15 -0.5 1,422,360 + LRK55_RS06580 0.15 +0.4 1,422,360 + LRK55_RS06580 0.15 -0.8 1,422,360 + LRK55_RS06580 0.15 +1.2 1,422,360 + LRK55_RS06580 0.15 -0.8 1,422,360 + LRK55_RS06580 0.15 -1.3 1,422,360 + LRK55_RS06580 0.15 -0.3 1,422,360 + LRK55_RS06580 0.15 -0.3 1,422,360 + LRK55_RS06580 0.15 +0.1 1,422,360 + LRK55_RS06580 0.15 -1.1 1,422,360 + LRK55_RS06580 0.15 -0.0 1,422,360 + LRK55_RS06580 0.15 +1.1 1,422,360 + LRK55_RS06580 0.15 -0.6 1,422,360 + LRK55_RS06580 0.15 +0.2 1,422,360 + LRK55_RS06580 0.15 -0.6 1,422,360 + LRK55_RS06580 0.15 +0.1 1,422,360 + LRK55_RS06580 0.15 -0.8 1,422,360 + LRK55_RS06580 0.15 -0.9 1,422,361 - LRK55_RS06580 0.15 +1.0 1,422,361 - LRK55_RS06580 0.15 -0.4 1,422,361 - LRK55_RS06580 0.15 -0.8 1,422,361 - LRK55_RS06580 0.15 -1.6 1,422,361 - LRK55_RS06580 0.15 -1.3 1,422,361 - LRK55_RS06580 0.15 -0.2 1,422,361 - LRK55_RS06580 0.15 -1.0 1,422,361 - LRK55_RS06580 0.15 -0.2 1,422,361 - LRK55_RS06580 0.15 +0.9 1,422,361 - LRK55_RS06580 0.15 -0.2 1,422,361 - LRK55_RS06580 0.15 -0.1 1,422,361 - LRK55_RS06580 0.15 -1.0 1,422,361 - LRK55_RS06580 0.15 +2.6 1,422,361 - LRK55_RS06580 0.15 -0.1 1,422,361 - LRK55_RS06580 0.15 -1.8 1,422,361 - LRK55_RS06580 0.15 -0.4 1,422,375 - LRK55_RS06580 0.18 -2.1
Or see this region's nucleotide sequence