Strain Fitness in Rhodanobacter denitrificans MT42 around LRK55_RS03460

Experiment: R2A_PIPES with Cadmium chloride 64 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntLRK55_RS03450 and LRK55_RS03455 are separated by 52 nucleotidesLRK55_RS03455 and LRK55_RS03460 overlap by 4 nucleotidesLRK55_RS03460 and LRK55_RS03465 are separated by 153 nucleotides LRK55_RS03450: LRK55_RS03450 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 731,744 to 733,126 _RS03450 LRK55_RS03455: LRK55_RS03455 - lipoprotein insertase outer membrane protein LolB, at 733,179 to 733,787 _RS03455 LRK55_RS03460: LRK55_RS03460 - tetratricopeptide repeat protein, at 733,784 to 735,472 _RS03460 LRK55_RS03465: LRK55_RS03465 - glutamyl-tRNA reductase, at 735,626 to 736,903 _RS03465 Position (kb) 733 734 735 736Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 732.856 kb on - strand, within LRK55_RS03450at 733.020 kb on + strandat 733.020 kb on + strandat 733.020 kb on + strandat 733.020 kb on + strandat 733.020 kb on + strandat 733.021 kb on - strandat 733.021 kb on - strandat 733.021 kb on - strandat 733.021 kb on - strandat 733.021 kb on - strandat 733.172 kb on + strandat 734.104 kb on - strand, within LRK55_RS03460at 734.200 kb on - strand, within LRK55_RS03460at 734.218 kb on - strand, within LRK55_RS03460at 734.284 kb on - strand, within LRK55_RS03460at 734.284 kb on - strand, within LRK55_RS03460at 734.284 kb on - strand, within LRK55_RS03460at 734.284 kb on - strand, within LRK55_RS03460at 734.302 kb on - strand, within LRK55_RS03460at 734.356 kb on - strand, within LRK55_RS03460at 734.479 kb on - strand, within LRK55_RS03460at 734.479 kb on - strand, within LRK55_RS03460at 734.536 kb on - strand, within LRK55_RS03460at 734.644 kb on - strand, within LRK55_RS03460at 734.644 kb on - strand, within LRK55_RS03460at 734.644 kb on - strand, within LRK55_RS03460at 734.695 kb on - strand, within LRK55_RS03460at 734.695 kb on - strand, within LRK55_RS03460at 734.724 kb on + strand, within LRK55_RS03460at 734.836 kb on - strand, within LRK55_RS03460at 734.836 kb on - strand, within LRK55_RS03460at 734.836 kb on - strand, within LRK55_RS03460at 734.836 kb on - strand, within LRK55_RS03460at 734.836 kb on - strand, within LRK55_RS03460at 734.836 kb on - strand, within LRK55_RS03460at 734.836 kb on - strand, within LRK55_RS03460at 734.836 kb on - strand, within LRK55_RS03460at 735.280 kb on - strand, within LRK55_RS03460at 735.473 kb on + strandat 735.473 kb on + strandat 735.473 kb on + strandat 735.473 kb on + strandat 735.473 kb on + strandat 735.473 kb on + strandat 735.473 kb on + strandat 735.473 kb on + strandat 735.474 kb on - strandat 735.474 kb on - strandat 735.474 kb on - strandat 735.474 kb on - strandat 735.474 kb on - strandat 735.474 kb on - strandat 735.482 kb on - strandat 735.482 kb on - strandat 735.581 kb on + strandat 735.581 kb on + strandat 735.581 kb on + strandat 735.582 kb on - strandat 735.586 kb on + strandat 735.586 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 64 uM
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732,856 - LRK55_RS03450 0.80 -0.4
733,020 + -2.1
733,020 + -1.5
733,020 + -0.8
733,020 + -0.2
733,020 + -1.9
733,021 - +1.0
733,021 - +1.4
733,021 - +1.1
733,021 - -0.4
733,021 - +0.7
733,172 + +0.5
734,104 - LRK55_RS03460 0.19 -0.8
734,200 - LRK55_RS03460 0.25 -1.6
734,218 - LRK55_RS03460 0.26 -0.4
734,284 - LRK55_RS03460 0.30 -3.8
734,284 - LRK55_RS03460 0.30 -2.5
734,284 - LRK55_RS03460 0.30 +2.2
734,284 - LRK55_RS03460 0.30 -0.4
734,302 - LRK55_RS03460 0.31 -1.6
734,356 - LRK55_RS03460 0.34 -2.8
734,479 - LRK55_RS03460 0.41 -2.3
734,479 - LRK55_RS03460 0.41 -0.8
734,536 - LRK55_RS03460 0.45 -2.5
734,644 - LRK55_RS03460 0.51 -2.0
734,644 - LRK55_RS03460 0.51 -1.8
734,644 - LRK55_RS03460 0.51 -1.8
734,695 - LRK55_RS03460 0.54 -2.5
734,695 - LRK55_RS03460 0.54 -1.6
734,724 + LRK55_RS03460 0.56 -2.1
734,836 - LRK55_RS03460 0.62 -2.5
734,836 - LRK55_RS03460 0.62 -1.4
734,836 - LRK55_RS03460 0.62 -1.8
734,836 - LRK55_RS03460 0.62 -3.7
734,836 - LRK55_RS03460 0.62 -2.4
734,836 - LRK55_RS03460 0.62 -2.1
734,836 - LRK55_RS03460 0.62 -3.1
734,836 - LRK55_RS03460 0.62 -2.8
735,280 - LRK55_RS03460 0.89 -2.1
735,473 + -2.5
735,473 + -1.1
735,473 + -1.6
735,473 + -1.4
735,473 + -3.9
735,473 + -1.1
735,473 + -2.1
735,473 + -0.2
735,474 - -1.8
735,474 - -1.1
735,474 - -1.6
735,474 - -2.3
735,474 - -1.4
735,474 - -0.1
735,482 - -1.8
735,482 - -0.8
735,581 + +2.4
735,581 + -0.5
735,581 + +0.1
735,582 - -0.8
735,586 + -1.6
735,586 + +0.6

Or see this region's nucleotide sequence