Experiment: R2A_PIPES with Cadmium chloride 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS03450 and LRK55_RS03455 are separated by 52 nucleotides LRK55_RS03455 and LRK55_RS03460 overlap by 4 nucleotides LRK55_RS03460 and LRK55_RS03465 are separated by 153 nucleotides
LRK55_RS03450: LRK55_RS03450 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 731,744 to 733,126
_RS03450
LRK55_RS03455: LRK55_RS03455 - lipoprotein insertase outer membrane protein LolB, at 733,179 to 733,787
_RS03455
LRK55_RS03460: LRK55_RS03460 - tetratricopeptide repeat protein, at 733,784 to 735,472
_RS03460
LRK55_RS03465: LRK55_RS03465 - glutamyl-tRNA reductase, at 735,626 to 736,903
_RS03465
Position (kb)
733
734
735
736 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 732.856 kb on - strand, within LRK55_RS03450 at 733.020 kb on + strand at 733.020 kb on + strand at 733.020 kb on + strand at 733.020 kb on + strand at 733.020 kb on + strand at 733.021 kb on - strand at 733.021 kb on - strand at 733.021 kb on - strand at 733.021 kb on - strand at 733.021 kb on - strand at 733.172 kb on + strand at 734.104 kb on - strand, within LRK55_RS03460 at 734.200 kb on - strand, within LRK55_RS03460 at 734.218 kb on - strand, within LRK55_RS03460 at 734.284 kb on - strand, within LRK55_RS03460 at 734.284 kb on - strand, within LRK55_RS03460 at 734.284 kb on - strand, within LRK55_RS03460 at 734.284 kb on - strand, within LRK55_RS03460 at 734.302 kb on - strand, within LRK55_RS03460 at 734.356 kb on - strand, within LRK55_RS03460 at 734.479 kb on - strand, within LRK55_RS03460 at 734.479 kb on - strand, within LRK55_RS03460 at 734.536 kb on - strand, within LRK55_RS03460 at 734.644 kb on - strand, within LRK55_RS03460 at 734.644 kb on - strand, within LRK55_RS03460 at 734.644 kb on - strand, within LRK55_RS03460 at 734.695 kb on - strand, within LRK55_RS03460 at 734.695 kb on - strand, within LRK55_RS03460 at 734.724 kb on + strand, within LRK55_RS03460 at 734.836 kb on - strand, within LRK55_RS03460 at 734.836 kb on - strand, within LRK55_RS03460 at 734.836 kb on - strand, within LRK55_RS03460 at 734.836 kb on - strand, within LRK55_RS03460 at 734.836 kb on - strand, within LRK55_RS03460 at 734.836 kb on - strand, within LRK55_RS03460 at 734.836 kb on - strand, within LRK55_RS03460 at 734.836 kb on - strand, within LRK55_RS03460 at 735.280 kb on - strand, within LRK55_RS03460 at 735.473 kb on + strand at 735.473 kb on + strand at 735.473 kb on + strand at 735.473 kb on + strand at 735.473 kb on + strand at 735.473 kb on + strand at 735.473 kb on + strand at 735.473 kb on + strand at 735.474 kb on - strand at 735.474 kb on - strand at 735.474 kb on - strand at 735.474 kb on - strand at 735.474 kb on - strand at 735.474 kb on - strand at 735.482 kb on - strand at 735.482 kb on - strand at 735.581 kb on + strand at 735.581 kb on + strand at 735.581 kb on + strand at 735.582 kb on - strand at 735.586 kb on + strand at 735.586 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 64 uM remove 732,856 - LRK55_RS03450 0.80 -0.4 733,020 + -2.1 733,020 + -1.5 733,020 + -0.8 733,020 + -0.2 733,020 + -1.9 733,021 - +1.0 733,021 - +1.4 733,021 - +1.1 733,021 - -0.4 733,021 - +0.7 733,172 + +0.5 734,104 - LRK55_RS03460 0.19 -0.8 734,200 - LRK55_RS03460 0.25 -1.6 734,218 - LRK55_RS03460 0.26 -0.4 734,284 - LRK55_RS03460 0.30 -3.8 734,284 - LRK55_RS03460 0.30 -2.5 734,284 - LRK55_RS03460 0.30 +2.2 734,284 - LRK55_RS03460 0.30 -0.4 734,302 - LRK55_RS03460 0.31 -1.6 734,356 - LRK55_RS03460 0.34 -2.8 734,479 - LRK55_RS03460 0.41 -2.3 734,479 - LRK55_RS03460 0.41 -0.8 734,536 - LRK55_RS03460 0.45 -2.5 734,644 - LRK55_RS03460 0.51 -2.0 734,644 - LRK55_RS03460 0.51 -1.8 734,644 - LRK55_RS03460 0.51 -1.8 734,695 - LRK55_RS03460 0.54 -2.5 734,695 - LRK55_RS03460 0.54 -1.6 734,724 + LRK55_RS03460 0.56 -2.1 734,836 - LRK55_RS03460 0.62 -2.5 734,836 - LRK55_RS03460 0.62 -1.4 734,836 - LRK55_RS03460 0.62 -1.8 734,836 - LRK55_RS03460 0.62 -3.7 734,836 - LRK55_RS03460 0.62 -2.4 734,836 - LRK55_RS03460 0.62 -2.1 734,836 - LRK55_RS03460 0.62 -3.1 734,836 - LRK55_RS03460 0.62 -2.8 735,280 - LRK55_RS03460 0.89 -2.1 735,473 + -2.5 735,473 + -1.1 735,473 + -1.6 735,473 + -1.4 735,473 + -3.9 735,473 + -1.1 735,473 + -2.1 735,473 + -0.2 735,474 - -1.8 735,474 - -1.1 735,474 - -1.6 735,474 - -2.3 735,474 - -1.4 735,474 - -0.1 735,482 - -1.8 735,482 - -0.8 735,581 + +2.4 735,581 + -0.5 735,581 + +0.1 735,582 - -0.8 735,586 + -1.6 735,586 + +0.6
Or see this region's nucleotide sequence