Experiment: R2A_PIPES with Cadmium chloride 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS03415 and LRK55_RS03420 are separated by 278 nucleotides LRK55_RS03420 and LRK55_RS03425 are separated by 139 nucleotides LRK55_RS03425 and LRK55_RS03430 are separated by 81 nucleotides LRK55_RS03430 and LRK55_RS03435 are separated by 54 nucleotides
LRK55_RS03415: LRK55_RS03415 - tRNA-Tyr, at 727,047 to 727,132
_RS03415
LRK55_RS03420: LRK55_RS03420 - acyl-CoA thioesterase, at 727,411 to 727,899
_RS03420
LRK55_RS03425: LRK55_RS03425 - redox-regulated ATPase YchF, at 728,039 to 729,127
_RS03425
LRK55_RS03430: LRK55_RS03430 - aminoacyl-tRNA hydrolase, at 729,209 to 729,790
_RS03430
LRK55_RS03435: LRK55_RS03435 - 50S ribosomal protein L25/general stress protein Ctc, at 729,845 to 730,510
_RS03435
Position (kb) 728
729
730 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 727.754 kb on + strand, within LRK55_RS03420 at 727.755 kb on - strand, within LRK55_RS03420 at 727.755 kb on - strand, within LRK55_RS03420 at 727.755 kb on - strand, within LRK55_RS03420 at 727.755 kb on - strand, within LRK55_RS03420 at 727.755 kb on - strand, within LRK55_RS03420 at 727.784 kb on + strand, within LRK55_RS03420 at 727.890 kb on + strand at 727.926 kb on + strand at 727.927 kb on - strand at 727.927 kb on - strand at 727.927 kb on - strand at 727.937 kb on + strand at 727.937 kb on + strand at 727.937 kb on + strand at 727.937 kb on + strand at 727.937 kb on + strand at 727.938 kb on - strand at 727.938 kb on - strand at 727.938 kb on - strand at 727.938 kb on - strand at 727.938 kb on - strand at 727.938 kb on - strand at 728.085 kb on + strand at 728.085 kb on + strand at 728.085 kb on + strand at 728.086 kb on - strand at 728.142 kb on + strand at 728.143 kb on - strand at 728.286 kb on + strand, within LRK55_RS03425 at 728.286 kb on + strand, within LRK55_RS03425 at 728.286 kb on + strand, within LRK55_RS03425 at 728.287 kb on - strand, within LRK55_RS03425 at 728.287 kb on - strand, within LRK55_RS03425 at 728.520 kb on + strand, within LRK55_RS03425 at 728.520 kb on + strand, within LRK55_RS03425 at 728.520 kb on + strand, within LRK55_RS03425 at 728.521 kb on - strand, within LRK55_RS03425 at 728.521 kb on - strand, within LRK55_RS03425 at 728.521 kb on - strand, within LRK55_RS03425 at 729.204 kb on - strand at 729.211 kb on - strand at 729.223 kb on + strand at 729.223 kb on + strand at 729.223 kb on + strand at 729.223 kb on + strand at 729.223 kb on + strand at 729.223 kb on + strand at 729.223 kb on + strand at 729.223 kb on + strand at 729.224 kb on - strand at 729.224 kb on - strand at 729.224 kb on - strand at 729.224 kb on - strand at 729.224 kb on - strand at 729.224 kb on - strand at 729.224 kb on - strand at 729.224 kb on - strand at 729.224 kb on - strand at 729.224 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 64 uM remove 727,754 + LRK55_RS03420 0.70 +0.1 727,755 - LRK55_RS03420 0.70 +0.7 727,755 - LRK55_RS03420 0.70 +0.5 727,755 - LRK55_RS03420 0.70 -0.5 727,755 - LRK55_RS03420 0.70 +0.2 727,755 - LRK55_RS03420 0.70 +0.1 727,784 + LRK55_RS03420 0.76 -2.4 727,890 + +0.4 727,926 + -2.3 727,927 - -1.6 727,927 - -1.1 727,927 - +1.8 727,937 + -3.0 727,937 + +0.8 727,937 + -0.4 727,937 + -1.3 727,937 + +0.1 727,938 - +0.0 727,938 - -0.1 727,938 - -2.0 727,938 - +1.2 727,938 - +0.2 727,938 - -0.8 728,085 + -0.8 728,085 + -1.8 728,085 + +0.5 728,086 - +1.2 728,142 + -1.4 728,143 - -0.2 728,286 + LRK55_RS03425 0.23 +1.2 728,286 + LRK55_RS03425 0.23 -0.4 728,286 + LRK55_RS03425 0.23 -0.8 728,287 - LRK55_RS03425 0.23 +0.2 728,287 - LRK55_RS03425 0.23 -1.1 728,520 + LRK55_RS03425 0.44 +0.2 728,520 + LRK55_RS03425 0.44 +0.2 728,520 + LRK55_RS03425 0.44 -1.6 728,521 - LRK55_RS03425 0.44 -0.4 728,521 - LRK55_RS03425 0.44 -0.4 728,521 - LRK55_RS03425 0.44 +2.1 729,204 - +0.5 729,211 - +1.5 729,223 + +2.8 729,223 + +0.2 729,223 + -0.2 729,223 + -0.8 729,223 + -1.8 729,223 + -1.6 729,223 + -0.8 729,223 + -0.5 729,224 - -2.6 729,224 - +0.8 729,224 - -0.1 729,224 - -0.3 729,224 - -1.3 729,224 - -0.9 729,224 - +0.0 729,224 - +0.1 729,224 - -0.3 729,224 - +0.6
Or see this region's nucleotide sequence