Experiment: R2A_PIPES with Cadmium chloride 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS01345 and LRK55_RS01350 are separated by 157 nucleotides LRK55_RS01350 and LRK55_RS01355 are separated by 65 nucleotides LRK55_RS01355 and LRK55_RS01360 are separated by 19 nucleotides
LRK55_RS01345: LRK55_RS01345 - carboxylesterase family protein, at 292,785 to 294,533
_RS01345
LRK55_RS01350: LRK55_RS01350 - LytTR family DNA-binding domain-containing protein, at 294,691 to 295,560
_RS01350
LRK55_RS01355: LRK55_RS01355 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 295,626 to 296,258
_RS01355
LRK55_RS01360: LRK55_RS01360 - imidazole glycerol phosphate synthase subunit HisF, at 296,278 to 297,048
_RS01360
Position (kb) 294
295
296 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 293.908 kb on + strand, within LRK55_RS01345 at 293.909 kb on - strand, within LRK55_RS01345 at 293.983 kb on + strand, within LRK55_RS01345 at 293.984 kb on - strand, within LRK55_RS01345 at 294.034 kb on + strand, within LRK55_RS01345 at 294.076 kb on + strand, within LRK55_RS01345 at 294.076 kb on + strand, within LRK55_RS01345 at 294.076 kb on + strand, within LRK55_RS01345 at 294.076 kb on + strand, within LRK55_RS01345 at 294.076 kb on + strand, within LRK55_RS01345 at 294.076 kb on + strand, within LRK55_RS01345 at 294.076 kb on + strand, within LRK55_RS01345 at 294.076 kb on + strand, within LRK55_RS01345 at 294.077 kb on - strand, within LRK55_RS01345 at 294.077 kb on - strand, within LRK55_RS01345 at 294.077 kb on - strand, within LRK55_RS01345 at 294.077 kb on - strand, within LRK55_RS01345 at 294.077 kb on - strand, within LRK55_RS01345 at 294.077 kb on - strand, within LRK55_RS01345 at 294.077 kb on - strand, within LRK55_RS01345 at 294.077 kb on - strand, within LRK55_RS01345 at 294.078 kb on + strand, within LRK55_RS01345 at 294.078 kb on + strand, within LRK55_RS01345 at 294.078 kb on + strand, within LRK55_RS01345 at 294.078 kb on + strand, within LRK55_RS01345 at 294.078 kb on + strand, within LRK55_RS01345 at 294.078 kb on + strand, within LRK55_RS01345 at 294.078 kb on + strand, within LRK55_RS01345 at 294.078 kb on + strand, within LRK55_RS01345 at 294.078 kb on + strand, within LRK55_RS01345 at 294.079 kb on - strand, within LRK55_RS01345 at 294.079 kb on - strand, within LRK55_RS01345 at 294.079 kb on - strand, within LRK55_RS01345 at 294.079 kb on - strand, within LRK55_RS01345 at 294.079 kb on - strand, within LRK55_RS01345 at 294.079 kb on - strand, within LRK55_RS01345 at 294.079 kb on - strand, within LRK55_RS01345 at 294.111 kb on + strand, within LRK55_RS01345 at 294.111 kb on + strand, within LRK55_RS01345 at 294.111 kb on + strand, within LRK55_RS01345 at 294.112 kb on - strand, within LRK55_RS01345 at 294.112 kb on - strand, within LRK55_RS01345 at 294.112 kb on - strand, within LRK55_RS01345 at 294.112 kb on - strand, within LRK55_RS01345 at 294.112 kb on - strand, within LRK55_RS01345 at 294.131 kb on + strand, within LRK55_RS01345 at 294.131 kb on + strand, within LRK55_RS01345 at 294.131 kb on + strand, within LRK55_RS01345 at 294.131 kb on + strand, within LRK55_RS01345 at 294.131 kb on + strand, within LRK55_RS01345 at 294.131 kb on + strand, within LRK55_RS01345 at 294.132 kb on - strand, within LRK55_RS01345 at 294.132 kb on - strand, within LRK55_RS01345 at 294.132 kb on - strand, within LRK55_RS01345 at 294.139 kb on + strand, within LRK55_RS01345 at 294.139 kb on + strand, within LRK55_RS01345 at 294.140 kb on - strand, within LRK55_RS01345 at 294.140 kb on - strand, within LRK55_RS01345 at 294.140 kb on - strand, within LRK55_RS01345 at 294.184 kb on + strand, within LRK55_RS01345 at 294.303 kb on + strand, within LRK55_RS01345 at 294.304 kb on - strand, within LRK55_RS01345 at 294.304 kb on - strand, within LRK55_RS01345 at 294.304 kb on - strand, within LRK55_RS01345 at 294.304 kb on - strand, within LRK55_RS01345 at 294.304 kb on - strand, within LRK55_RS01345 at 294.304 kb on - strand, within LRK55_RS01345 at 294.321 kb on + strand, within LRK55_RS01345 at 294.321 kb on + strand, within LRK55_RS01345 at 294.321 kb on + strand, within LRK55_RS01345 at 294.321 kb on + strand, within LRK55_RS01345 at 294.322 kb on - strand, within LRK55_RS01345 at 294.322 kb on - strand, within LRK55_RS01345 at 294.322 kb on - strand, within LRK55_RS01345 at 294.322 kb on - strand, within LRK55_RS01345 at 294.338 kb on + strand, within LRK55_RS01345 at 294.338 kb on + strand, within LRK55_RS01345 at 294.338 kb on + strand, within LRK55_RS01345 at 294.473 kb on + strand at 294.473 kb on + strand at 294.473 kb on + strand at 294.474 kb on - strand at 294.474 kb on - strand at 294.571 kb on - strand at 294.591 kb on + strand at 294.773 kb on + strand at 294.773 kb on + strand at 294.773 kb on + strand at 294.773 kb on + strand at 294.774 kb on - strand at 294.774 kb on - strand at 294.774 kb on - strand at 294.774 kb on - strand at 294.774 kb on - strand at 294.774 kb on - strand at 294.774 kb on - strand at 294.774 kb on - strand at 294.775 kb on + strand at 294.775 kb on + strand at 294.797 kb on + strand, within LRK55_RS01350 at 294.797 kb on + strand, within LRK55_RS01350 at 294.838 kb on + strand, within LRK55_RS01350 at 294.839 kb on - strand, within LRK55_RS01350 at 295.119 kb on - strand, within LRK55_RS01350 at 295.154 kb on + strand, within LRK55_RS01350 at 295.155 kb on - strand, within LRK55_RS01350 at 295.315 kb on + strand, within LRK55_RS01350 at 295.315 kb on + strand, within LRK55_RS01350 at 295.316 kb on - strand, within LRK55_RS01350 at 295.389 kb on + strand, within LRK55_RS01350 at 295.389 kb on + strand, within LRK55_RS01350 at 295.390 kb on - strand, within LRK55_RS01350 at 295.390 kb on - strand, within LRK55_RS01350 at 295.390 kb on - strand, within LRK55_RS01350 at 295.478 kb on + strand at 295.522 kb on + strand at 295.522 kb on + strand at 295.523 kb on - strand at 295.523 kb on - strand at 295.558 kb on + strand at 295.559 kb on - strand at 295.559 kb on - strand at 295.576 kb on - strand at 295.676 kb on - strand at 295.767 kb on + strand, within LRK55_RS01355 at 295.768 kb on - strand, within LRK55_RS01355 at 295.852 kb on + strand, within LRK55_RS01355 at 295.853 kb on - strand, within LRK55_RS01355 at 295.853 kb on - strand, within LRK55_RS01355 at 296.003 kb on + strand, within LRK55_RS01355 at 296.004 kb on - strand, within LRK55_RS01355 at 296.004 kb on - strand, within LRK55_RS01355 at 296.149 kb on + strand, within LRK55_RS01355 at 296.149 kb on + strand, within LRK55_RS01355 at 296.150 kb on - strand, within LRK55_RS01355 at 296.163 kb on + strand, within LRK55_RS01355 at 296.163 kb on + strand, within LRK55_RS01355 at 296.163 kb on + strand, within LRK55_RS01355 at 296.164 kb on - strand, within LRK55_RS01355 at 296.164 kb on - strand, within LRK55_RS01355 at 296.164 kb on - strand, within LRK55_RS01355 at 296.312 kb on + strand at 296.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 64 uM remove 293,908 + LRK55_RS01345 0.64 +1.1 293,909 - LRK55_RS01345 0.64 +0.6 293,983 + LRK55_RS01345 0.68 +0.3 293,984 - LRK55_RS01345 0.69 -0.6 294,034 + LRK55_RS01345 0.71 -1.1 294,076 + LRK55_RS01345 0.74 +1.9 294,076 + LRK55_RS01345 0.74 -1.0 294,076 + LRK55_RS01345 0.74 +1.1 294,076 + LRK55_RS01345 0.74 -1.3 294,076 + LRK55_RS01345 0.74 -0.3 294,076 + LRK55_RS01345 0.74 +0.0 294,076 + LRK55_RS01345 0.74 +0.3 294,076 + LRK55_RS01345 0.74 +0.4 294,077 - LRK55_RS01345 0.74 -0.6 294,077 - LRK55_RS01345 0.74 -1.9 294,077 - LRK55_RS01345 0.74 +1.6 294,077 - LRK55_RS01345 0.74 -2.5 294,077 - LRK55_RS01345 0.74 +1.4 294,077 - LRK55_RS01345 0.74 +0.3 294,077 - LRK55_RS01345 0.74 -0.9 294,077 - LRK55_RS01345 0.74 +0.9 294,078 + LRK55_RS01345 0.74 +1.4 294,078 + LRK55_RS01345 0.74 -1.6 294,078 + LRK55_RS01345 0.74 -0.2 294,078 + LRK55_RS01345 0.74 -0.5 294,078 + LRK55_RS01345 0.74 -0.4 294,078 + LRK55_RS01345 0.74 -2.8 294,078 + LRK55_RS01345 0.74 -0.9 294,078 + LRK55_RS01345 0.74 -0.1 294,078 + LRK55_RS01345 0.74 +1.3 294,079 - LRK55_RS01345 0.74 -1.0 294,079 - LRK55_RS01345 0.74 +0.6 294,079 - LRK55_RS01345 0.74 +0.1 294,079 - LRK55_RS01345 0.74 +0.2 294,079 - LRK55_RS01345 0.74 +0.8 294,079 - LRK55_RS01345 0.74 +0.1 294,079 - LRK55_RS01345 0.74 +0.9 294,111 + LRK55_RS01345 0.76 -2.4 294,111 + LRK55_RS01345 0.76 -0.9 294,111 + LRK55_RS01345 0.76 +0.9 294,112 - LRK55_RS01345 0.76 -1.1 294,112 - LRK55_RS01345 0.76 -2.1 294,112 - LRK55_RS01345 0.76 -0.7 294,112 - LRK55_RS01345 0.76 +0.8 294,112 - LRK55_RS01345 0.76 +0.2 294,131 + LRK55_RS01345 0.77 -0.6 294,131 + LRK55_RS01345 0.77 -2.5 294,131 + LRK55_RS01345 0.77 -2.3 294,131 + LRK55_RS01345 0.77 -1.3 294,131 + LRK55_RS01345 0.77 -0.1 294,131 + LRK55_RS01345 0.77 +0.7 294,132 - LRK55_RS01345 0.77 -2.6 294,132 - LRK55_RS01345 0.77 +0.5 294,132 - LRK55_RS01345 0.77 +0.2 294,139 + LRK55_RS01345 0.77 +0.6 294,139 + LRK55_RS01345 0.77 -1.2 294,140 - LRK55_RS01345 0.77 +2.3 294,140 - LRK55_RS01345 0.77 +0.7 294,140 - LRK55_RS01345 0.77 -0.3 294,184 + LRK55_RS01345 0.80 +1.2 294,303 + LRK55_RS01345 0.87 +0.5 294,304 - LRK55_RS01345 0.87 +1.4 294,304 - LRK55_RS01345 0.87 -0.7 294,304 - LRK55_RS01345 0.87 +0.0 294,304 - LRK55_RS01345 0.87 +0.9 294,304 - LRK55_RS01345 0.87 +1.5 294,304 - LRK55_RS01345 0.87 -2.6 294,321 + LRK55_RS01345 0.88 -2.4 294,321 + LRK55_RS01345 0.88 +0.5 294,321 + LRK55_RS01345 0.88 +0.4 294,321 + LRK55_RS01345 0.88 +0.8 294,322 - LRK55_RS01345 0.88 -0.9 294,322 - LRK55_RS01345 0.88 -2.9 294,322 - LRK55_RS01345 0.88 +0.4 294,322 - LRK55_RS01345 0.88 +0.3 294,338 + LRK55_RS01345 0.89 -0.7 294,338 + LRK55_RS01345 0.89 -2.2 294,338 + LRK55_RS01345 0.89 -0.3 294,473 + -1.4 294,473 + -0.2 294,473 + -1.5 294,474 - -0.5 294,474 - +0.4 294,571 - -2.3 294,591 + -0.4 294,773 + +0.6 294,773 + +1.1 294,773 + +0.1 294,773 + +0.1 294,774 - +1.1 294,774 - -0.2 294,774 - -1.0 294,774 - -0.3 294,774 - -0.5 294,774 - +1.1 294,774 - +1.2 294,774 - -1.0 294,775 + -0.7 294,775 + -0.2 294,797 + LRK55_RS01350 0.12 -0.1 294,797 + LRK55_RS01350 0.12 +0.3 294,838 + LRK55_RS01350 0.17 -1.0 294,839 - LRK55_RS01350 0.17 +1.2 295,119 - LRK55_RS01350 0.49 -1.7 295,154 + LRK55_RS01350 0.53 -0.5 295,155 - LRK55_RS01350 0.53 -0.7 295,315 + LRK55_RS01350 0.72 -0.1 295,315 + LRK55_RS01350 0.72 -1.9 295,316 - LRK55_RS01350 0.72 -0.7 295,389 + LRK55_RS01350 0.80 -0.9 295,389 + LRK55_RS01350 0.80 +0.0 295,390 - LRK55_RS01350 0.80 +0.6 295,390 - LRK55_RS01350 0.80 -1.8 295,390 - LRK55_RS01350 0.80 -0.2 295,478 + +1.2 295,522 + +0.3 295,522 + -1.1 295,523 - -0.4 295,523 - -0.5 295,558 + +2.1 295,559 - -0.9 295,559 - -0.1 295,576 - -0.2 295,676 - -1.3 295,767 + LRK55_RS01355 0.22 +1.5 295,768 - LRK55_RS01355 0.22 +0.3 295,852 + LRK55_RS01355 0.36 -0.2 295,853 - LRK55_RS01355 0.36 -0.3 295,853 - LRK55_RS01355 0.36 +0.7 296,003 + LRK55_RS01355 0.60 +0.7 296,004 - LRK55_RS01355 0.60 +1.0 296,004 - LRK55_RS01355 0.60 +0.1 296,149 + LRK55_RS01355 0.83 +2.6 296,149 + LRK55_RS01355 0.83 +0.1 296,150 - LRK55_RS01355 0.83 +2.2 296,163 + LRK55_RS01355 0.85 +0.9 296,163 + LRK55_RS01355 0.85 +0.5 296,163 + LRK55_RS01355 0.85 +0.8 296,164 - LRK55_RS01355 0.85 +3.3 296,164 - LRK55_RS01355 0.85 +0.9 296,164 - LRK55_RS01355 0.85 +3.0 296,312 + -0.6 296,313 - +2.1
Or see this region's nucleotide sequence