Experiment: R2A_PIPES with Cadmium chloride 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS01070 and LRK55_RS01075 are separated by 166 nucleotides LRK55_RS01075 and LRK55_RS01080 are separated by 149 nucleotides LRK55_RS01080 and LRK55_RS01085 are separated by 59 nucleotides
LRK55_RS01070: LRK55_RS01070 - histidine--tRNA ligase, at 231,712 to 233,091
_RS01070
LRK55_RS01075: LRK55_RS01075 - tRNA-Pro, at 233,258 to 233,334
_RS01075
LRK55_RS01080: LRK55_RS01080 - BON domain-containing protein, at 233,484 to 234,134
_RS01080
LRK55_RS01085: LRK55_RS01085 - zinc-dependent alcohol dehydrogenase family protein, at 234,194 to 235,177
_RS01085
Position (kb) 233
234
235 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 233.102 kb on - strand at 233.102 kb on - strand at 233.160 kb on + strand at 233.445 kb on + strand at 233.446 kb on - strand at 233.446 kb on - strand at 233.610 kb on + strand, within LRK55_RS01080 at 233.611 kb on - strand, within LRK55_RS01080 at 233.611 kb on - strand, within LRK55_RS01080 at 233.611 kb on - strand, within LRK55_RS01080 at 233.611 kb on - strand, within LRK55_RS01080 at 233.838 kb on + strand, within LRK55_RS01080 at 233.838 kb on + strand, within LRK55_RS01080 at 233.838 kb on + strand, within LRK55_RS01080 at 233.838 kb on + strand, within LRK55_RS01080 at 233.839 kb on - strand, within LRK55_RS01080 at 233.839 kb on - strand, within LRK55_RS01080 at 233.839 kb on - strand, within LRK55_RS01080 at 234.482 kb on + strand, within LRK55_RS01085 at 234.684 kb on + strand, within LRK55_RS01085 at 234.684 kb on + strand, within LRK55_RS01085 at 234.685 kb on - strand, within LRK55_RS01085 at 234.685 kb on - strand, within LRK55_RS01085 at 234.685 kb on - strand, within LRK55_RS01085 at 234.685 kb on - strand, within LRK55_RS01085 at 234.802 kb on - strand, within LRK55_RS01085 at 234.802 kb on - strand, within LRK55_RS01085 at 234.849 kb on + strand, within LRK55_RS01085 at 234.868 kb on + strand, within LRK55_RS01085
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 64 uM remove 233,102 - +0.8 233,102 - +1.0 233,160 + -0.5 233,445 + -1.2 233,446 - -2.5 233,446 - -2.9 233,610 + LRK55_RS01080 0.19 +0.2 233,611 - LRK55_RS01080 0.20 -1.4 233,611 - LRK55_RS01080 0.20 -0.7 233,611 - LRK55_RS01080 0.20 -0.0 233,611 - LRK55_RS01080 0.20 +0.2 233,838 + LRK55_RS01080 0.54 +1.2 233,838 + LRK55_RS01080 0.54 +0.2 233,838 + LRK55_RS01080 0.54 -0.2 233,838 + LRK55_RS01080 0.54 -1.7 233,839 - LRK55_RS01080 0.55 -0.9 233,839 - LRK55_RS01080 0.55 +0.1 233,839 - LRK55_RS01080 0.55 -0.2 234,482 + LRK55_RS01085 0.29 +1.4 234,684 + LRK55_RS01085 0.50 +0.1 234,684 + LRK55_RS01085 0.50 -2.0 234,685 - LRK55_RS01085 0.50 +0.8 234,685 - LRK55_RS01085 0.50 +0.6 234,685 - LRK55_RS01085 0.50 +0.1 234,685 - LRK55_RS01085 0.50 -0.0 234,802 - LRK55_RS01085 0.62 -1.2 234,802 - LRK55_RS01085 0.62 +1.1 234,849 + LRK55_RS01085 0.67 +1.4 234,868 + LRK55_RS01085 0.68 -1.7
Or see this region's nucleotide sequence