Experiment: R2A_PIPES with Cadmium chloride 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS17335 and LRK55_RS17340 overlap by 4 nucleotides LRK55_RS17340 and LRK55_RS17345 are separated by 139 nucleotides LRK55_RS17345 and LRK55_RS17350 are separated by 81 nucleotides LRK55_RS17350 and LRK55_RS17355 overlap by 4 nucleotides
LRK55_RS17335: LRK55_RS17335 - efflux RND transporter periplasmic adaptor subunit, at 3,762,559 to 3,763,656
_RS17335
LRK55_RS17340: LRK55_RS17340 - TolC family protein, at 3,763,653 to 3,764,831
_RS17340
LRK55_RS17345: LRK55_RS17345 - hypothetical protein, at 3,764,971 to 3,765,441
_RS17345
LRK55_RS17350: LRK55_RS17350 - hypothetical protein, at 3,765,523 to 3,765,831
_RS17350
LRK55_RS17355: LRK55_RS17355 - tyrosine-type recombinase/integrase, at 3,765,828 to 3,766,991
_RS17355
Position (kb)
3763
3764
3765 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3763.241 kb on + strand, within LRK55_RS17335 at 3763.241 kb on + strand, within LRK55_RS17335 at 3763.242 kb on - strand, within LRK55_RS17335 at 3763.403 kb on - strand, within LRK55_RS17335 at 3763.435 kb on + strand, within LRK55_RS17335 at 3763.436 kb on - strand, within LRK55_RS17335 at 3763.436 kb on - strand, within LRK55_RS17335 at 3763.436 kb on - strand, within LRK55_RS17335 at 3763.445 kb on + strand, within LRK55_RS17335 at 3763.445 kb on + strand, within LRK55_RS17335 at 3763.445 kb on + strand, within LRK55_RS17335 at 3763.445 kb on + strand, within LRK55_RS17335 at 3763.673 kb on + strand at 3763.673 kb on + strand at 3763.748 kb on - strand at 3763.824 kb on + strand, within LRK55_RS17340 at 3763.824 kb on + strand, within LRK55_RS17340 at 3763.831 kb on + strand, within LRK55_RS17340 at 3764.285 kb on + strand, within LRK55_RS17340 at 3764.285 kb on + strand, within LRK55_RS17340 at 3764.285 kb on + strand, within LRK55_RS17340 at 3764.285 kb on + strand, within LRK55_RS17340 at 3764.285 kb on + strand, within LRK55_RS17340 at 3764.285 kb on + strand, within LRK55_RS17340 at 3764.285 kb on + strand, within LRK55_RS17340 at 3764.285 kb on + strand, within LRK55_RS17340 at 3764.286 kb on - strand, within LRK55_RS17340 at 3764.286 kb on - strand, within LRK55_RS17340 at 3764.286 kb on - strand, within LRK55_RS17340 at 3764.286 kb on - strand, within LRK55_RS17340 at 3764.286 kb on - strand, within LRK55_RS17340 at 3764.286 kb on - strand, within LRK55_RS17340 at 3764.286 kb on - strand, within LRK55_RS17340 at 3764.286 kb on - strand, within LRK55_RS17340 at 3764.588 kb on + strand, within LRK55_RS17340 at 3764.588 kb on + strand, within LRK55_RS17340 at 3764.589 kb on - strand, within LRK55_RS17340 at 3764.590 kb on + strand, within LRK55_RS17340 at 3764.590 kb on + strand, within LRK55_RS17340 at 3764.591 kb on - strand, within LRK55_RS17340 at 3764.591 kb on - strand, within LRK55_RS17340 at 3764.591 kb on - strand, within LRK55_RS17340 at 3764.684 kb on + strand, within LRK55_RS17340 at 3764.734 kb on + strand at 3764.734 kb on + strand at 3764.734 kb on + strand at 3764.734 kb on + strand at 3764.734 kb on + strand at 3764.734 kb on + strand at 3764.734 kb on + strand at 3764.734 kb on + strand at 3764.735 kb on - strand at 3764.735 kb on - strand at 3764.735 kb on - strand at 3764.735 kb on - strand at 3764.735 kb on - strand at 3764.735 kb on - strand at 3764.927 kb on - strand at 3764.927 kb on - strand at 3765.014 kb on + strand at 3765.210 kb on + strand, within LRK55_RS17345 at 3765.210 kb on + strand, within LRK55_RS17345 at 3765.210 kb on + strand, within LRK55_RS17345 at 3765.210 kb on + strand, within LRK55_RS17345 at 3765.210 kb on + strand, within LRK55_RS17345 at 3765.210 kb on + strand, within LRK55_RS17345 at 3765.211 kb on - strand, within LRK55_RS17345 at 3765.211 kb on - strand, within LRK55_RS17345 at 3765.211 kb on - strand, within LRK55_RS17345 at 3765.211 kb on - strand, within LRK55_RS17345 at 3765.430 kb on + strand at 3765.430 kb on + strand at 3765.430 kb on + strand at 3765.431 kb on - strand at 3765.431 kb on - strand at 3765.431 kb on - strand at 3765.431 kb on - strand at 3765.431 kb on - strand at 3765.431 kb on - strand at 3765.437 kb on + strand at 3765.437 kb on + strand at 3765.437 kb on + strand at 3765.437 kb on + strand at 3765.437 kb on + strand at 3765.437 kb on + strand at 3765.437 kb on + strand at 3765.437 kb on + strand at 3765.438 kb on - strand at 3765.438 kb on - strand at 3765.438 kb on - strand at 3765.438 kb on - strand at 3765.438 kb on - strand at 3765.438 kb on - strand at 3765.438 kb on - strand at 3765.438 kb on - strand at 3765.438 kb on - strand at 3765.445 kb on + strand at 3765.446 kb on - strand at 3765.447 kb on + strand at 3765.447 kb on + strand at 3765.448 kb on - strand at 3765.448 kb on - strand at 3765.448 kb on - strand at 3765.536 kb on + strand at 3765.536 kb on + strand at 3765.537 kb on - strand at 3765.601 kb on + strand, within LRK55_RS17350 at 3765.601 kb on + strand, within LRK55_RS17350 at 3765.601 kb on + strand, within LRK55_RS17350 at 3765.601 kb on + strand, within LRK55_RS17350 at 3765.601 kb on + strand, within LRK55_RS17350 at 3765.601 kb on + strand, within LRK55_RS17350 at 3765.601 kb on + strand, within LRK55_RS17350 at 3765.602 kb on - strand, within LRK55_RS17350 at 3765.664 kb on - strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.731 kb on + strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.732 kb on - strand, within LRK55_RS17350 at 3765.756 kb on + strand, within LRK55_RS17350 at 3765.756 kb on + strand, within LRK55_RS17350 at 3765.756 kb on + strand, within LRK55_RS17350 at 3765.756 kb on + strand, within LRK55_RS17350 at 3765.756 kb on + strand, within LRK55_RS17350 at 3765.757 kb on - strand, within LRK55_RS17350
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 64 uM remove 3,763,241 + LRK55_RS17335 0.62 +0.9 3,763,241 + LRK55_RS17335 0.62 -2.2 3,763,242 - LRK55_RS17335 0.62 -0.5 3,763,403 - LRK55_RS17335 0.77 +0.1 3,763,435 + LRK55_RS17335 0.80 -0.4 3,763,436 - LRK55_RS17335 0.80 +0.3 3,763,436 - LRK55_RS17335 0.80 +2.0 3,763,436 - LRK55_RS17335 0.80 -2.3 3,763,445 + LRK55_RS17335 0.81 +1.1 3,763,445 + LRK55_RS17335 0.81 -1.7 3,763,445 + LRK55_RS17335 0.81 +1.3 3,763,445 + LRK55_RS17335 0.81 +1.8 3,763,673 + +1.7 3,763,673 + +0.5 3,763,748 - -2.5 3,763,824 + LRK55_RS17340 0.15 +0.9 3,763,824 + LRK55_RS17340 0.15 +0.5 3,763,831 + LRK55_RS17340 0.15 +1.9 3,764,285 + LRK55_RS17340 0.54 +1.3 3,764,285 + LRK55_RS17340 0.54 -0.0 3,764,285 + LRK55_RS17340 0.54 +2.1 3,764,285 + LRK55_RS17340 0.54 -0.2 3,764,285 + LRK55_RS17340 0.54 +1.5 3,764,285 + LRK55_RS17340 0.54 -1.3 3,764,285 + LRK55_RS17340 0.54 -1.5 3,764,285 + LRK55_RS17340 0.54 +1.9 3,764,286 - LRK55_RS17340 0.54 -2.1 3,764,286 - LRK55_RS17340 0.54 +1.3 3,764,286 - LRK55_RS17340 0.54 -2.2 3,764,286 - LRK55_RS17340 0.54 -1.0 3,764,286 - LRK55_RS17340 0.54 +1.2 3,764,286 - LRK55_RS17340 0.54 +2.3 3,764,286 - LRK55_RS17340 0.54 -1.5 3,764,286 - LRK55_RS17340 0.54 +0.1 3,764,588 + LRK55_RS17340 0.79 +1.4 3,764,588 + LRK55_RS17340 0.79 +0.4 3,764,589 - LRK55_RS17340 0.79 -3.0 3,764,590 + LRK55_RS17340 0.79 -2.2 3,764,590 + LRK55_RS17340 0.79 +1.5 3,764,591 - LRK55_RS17340 0.80 -2.0 3,764,591 - LRK55_RS17340 0.80 +0.0 3,764,591 - LRK55_RS17340 0.80 +1.2 3,764,684 + LRK55_RS17340 0.87 +1.3 3,764,734 + -0.4 3,764,734 + +1.4 3,764,734 + +2.7 3,764,734 + +0.1 3,764,734 + -0.2 3,764,734 + +2.5 3,764,734 + +1.9 3,764,734 + +1.7 3,764,735 - -0.7 3,764,735 - -2.6 3,764,735 - +0.7 3,764,735 - +1.7 3,764,735 - +3.2 3,764,735 - -1.0 3,764,927 - -0.1 3,764,927 - +1.9 3,765,014 + +0.7 3,765,210 + LRK55_RS17345 0.51 +1.3 3,765,210 + LRK55_RS17345 0.51 -1.0 3,765,210 + LRK55_RS17345 0.51 +1.4 3,765,210 + LRK55_RS17345 0.51 -0.3 3,765,210 + LRK55_RS17345 0.51 -0.2 3,765,210 + LRK55_RS17345 0.51 +0.0 3,765,211 - LRK55_RS17345 0.51 +0.1 3,765,211 - LRK55_RS17345 0.51 +1.8 3,765,211 - LRK55_RS17345 0.51 -2.5 3,765,211 - LRK55_RS17345 0.51 +1.1 3,765,430 + -0.3 3,765,430 + +1.0 3,765,430 + -0.8 3,765,431 - +0.7 3,765,431 - +2.3 3,765,431 - +2.1 3,765,431 - +1.1 3,765,431 - +0.3 3,765,431 - +2.0 3,765,437 + +0.8 3,765,437 + +1.1 3,765,437 + +0.7 3,765,437 + +1.6 3,765,437 + +1.4 3,765,437 + +1.8 3,765,437 + -0.5 3,765,437 + +3.2 3,765,438 - +0.5 3,765,438 - +0.7 3,765,438 - -3.1 3,765,438 - +0.5 3,765,438 - +1.3 3,765,438 - +0.9 3,765,438 - +2.4 3,765,438 - +1.8 3,765,438 - -0.8 3,765,445 + +1.1 3,765,446 - +1.4 3,765,447 + +0.8 3,765,447 + -2.9 3,765,448 - -0.7 3,765,448 - -1.9 3,765,448 - -2.2 3,765,536 + -1.1 3,765,536 + +0.3 3,765,537 - -1.3 3,765,601 + LRK55_RS17350 0.25 -0.6 3,765,601 + LRK55_RS17350 0.25 -2.2 3,765,601 + LRK55_RS17350 0.25 +0.4 3,765,601 + LRK55_RS17350 0.25 +0.8 3,765,601 + LRK55_RS17350 0.25 -0.2 3,765,601 + LRK55_RS17350 0.25 -1.7 3,765,601 + LRK55_RS17350 0.25 +0.6 3,765,602 - LRK55_RS17350 0.26 -1.6 3,765,664 - LRK55_RS17350 0.46 -0.4 3,765,731 + LRK55_RS17350 0.67 +0.9 3,765,731 + LRK55_RS17350 0.67 +0.4 3,765,731 + LRK55_RS17350 0.67 -2.2 3,765,731 + LRK55_RS17350 0.67 +1.3 3,765,731 + LRK55_RS17350 0.67 +0.0 3,765,731 + LRK55_RS17350 0.67 +0.1 3,765,731 + LRK55_RS17350 0.67 +0.7 3,765,731 + LRK55_RS17350 0.67 +0.9 3,765,731 + LRK55_RS17350 0.67 +0.1 3,765,731 + LRK55_RS17350 0.67 -0.3 3,765,731 + LRK55_RS17350 0.67 -2.2 3,765,731 + LRK55_RS17350 0.67 +0.6 3,765,731 + LRK55_RS17350 0.67 +1.3 3,765,731 + LRK55_RS17350 0.67 -1.7 3,765,731 + LRK55_RS17350 0.67 -0.3 3,765,731 + LRK55_RS17350 0.67 -2.0 3,765,731 + LRK55_RS17350 0.67 +0.9 3,765,731 + LRK55_RS17350 0.67 +0.6 3,765,731 + LRK55_RS17350 0.67 -0.1 3,765,731 + LRK55_RS17350 0.67 +0.1 3,765,731 + LRK55_RS17350 0.67 +0.3 3,765,731 + LRK55_RS17350 0.67 -0.6 3,765,731 + LRK55_RS17350 0.67 +1.6 3,765,731 + LRK55_RS17350 0.67 +1.0 3,765,731 + LRK55_RS17350 0.67 +1.8 3,765,731 + LRK55_RS17350 0.67 +1.2 3,765,731 + LRK55_RS17350 0.67 -2.2 3,765,732 - LRK55_RS17350 0.68 +1.5 3,765,732 - LRK55_RS17350 0.68 +0.9 3,765,732 - LRK55_RS17350 0.68 -2.7 3,765,732 - LRK55_RS17350 0.68 -2.6 3,765,732 - LRK55_RS17350 0.68 -1.7 3,765,732 - LRK55_RS17350 0.68 -1.2 3,765,732 - LRK55_RS17350 0.68 -3.0 3,765,732 - LRK55_RS17350 0.68 -3.5 3,765,732 - LRK55_RS17350 0.68 -0.3 3,765,732 - LRK55_RS17350 0.68 -3.0 3,765,732 - LRK55_RS17350 0.68 +1.9 3,765,732 - LRK55_RS17350 0.68 -0.3 3,765,732 - LRK55_RS17350 0.68 +0.9 3,765,732 - LRK55_RS17350 0.68 +0.0 3,765,732 - LRK55_RS17350 0.68 -0.7 3,765,732 - LRK55_RS17350 0.68 -0.0 3,765,732 - LRK55_RS17350 0.68 -1.5 3,765,732 - LRK55_RS17350 0.68 -0.3 3,765,732 - LRK55_RS17350 0.68 -0.1 3,765,732 - LRK55_RS17350 0.68 -0.5 3,765,732 - LRK55_RS17350 0.68 -0.7 3,765,732 - LRK55_RS17350 0.68 +0.6 3,765,732 - LRK55_RS17350 0.68 -2.4 3,765,732 - LRK55_RS17350 0.68 -2.0 3,765,732 - LRK55_RS17350 0.68 +0.0 3,765,732 - LRK55_RS17350 0.68 -0.3 3,765,732 - LRK55_RS17350 0.68 +0.8 3,765,732 - LRK55_RS17350 0.68 -1.0 3,765,756 + LRK55_RS17350 0.75 +0.7 3,765,756 + LRK55_RS17350 0.75 -3.5 3,765,756 + LRK55_RS17350 0.75 -1.5 3,765,756 + LRK55_RS17350 0.75 +1.8 3,765,756 + LRK55_RS17350 0.75 +0.5 3,765,757 - LRK55_RS17350 0.76 +0.3
Or see this region's nucleotide sequence