Experiment: R2A_PIPES with Cadmium chloride 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS09035 and LRK55_RS09040 are separated by 182 nucleotides LRK55_RS09040 and LRK55_RS09045 are separated by 9 nucleotides LRK55_RS09045 and LRK55_RS09050 are separated by 363 nucleotides LRK55_RS09050 and LRK55_RS09055 are separated by 132 nucleotides LRK55_RS09055 and LRK55_RS09060 overlap by 8 nucleotides
LRK55_RS09035: LRK55_RS09035 - DNA (cytosine-5-)-methyltransferase, at 1,981,921 to 1,983,483
_RS09035
LRK55_RS09040: LRK55_RS09040 - very short patch repair endonuclease, at 1,983,666 to 1,984,070
_RS09040
LRK55_RS09045: LRK55_RS09045 - IS3 family transposase, at 1,984,080 to 1,985,095
_RS09045
LRK55_RS09050: LRK55_RS09050 - helix-turn-helix transcriptional regulator, at 1,985,459 to 1,985,710
_RS09050
LRK55_RS09055: LRK55_RS09055 - hypothetical protein, at 1,985,843 to 1,986,079
_RS09055
LRK55_RS09060: LRK55_RS09060 - site-specific integrase, at 1,986,072 to 1,987,400
_RS09060
Position (kb)
1984
1985
1986 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1983.206 kb on + strand, within LRK55_RS09035 at 1983.275 kb on + strand, within LRK55_RS09035 at 1983.278 kb on + strand, within LRK55_RS09035 at 1983.278 kb on + strand, within LRK55_RS09035 at 1983.278 kb on + strand, within LRK55_RS09035 at 1983.320 kb on + strand, within LRK55_RS09035 at 1983.511 kb on - strand at 1983.519 kb on + strand at 1983.519 kb on + strand at 1983.520 kb on - strand at 1983.520 kb on - strand at 1983.520 kb on - strand at 1983.520 kb on - strand at 1983.520 kb on - strand at 1983.520 kb on - strand at 1983.544 kb on + strand at 1983.544 kb on + strand at 1983.545 kb on - strand at 1983.545 kb on - strand at 1983.573 kb on - strand at 1983.666 kb on - strand at 1983.683 kb on + strand at 1983.684 kb on - strand at 1983.970 kb on + strand, within LRK55_RS09040 at 1983.970 kb on + strand, within LRK55_RS09040 at 1983.970 kb on + strand, within LRK55_RS09040 at 1983.970 kb on + strand, within LRK55_RS09040 at 1983.970 kb on + strand, within LRK55_RS09040 at 1983.970 kb on + strand, within LRK55_RS09040 at 1983.971 kb on - strand, within LRK55_RS09040 at 1984.009 kb on + strand, within LRK55_RS09040 at 1984.010 kb on - strand, within LRK55_RS09040 at 1985.201 kb on + strand at 1985.378 kb on + strand at 1985.378 kb on + strand at 1985.379 kb on - strand at 1985.379 kb on - strand at 1985.606 kb on - strand, within LRK55_RS09050 at 1985.606 kb on - strand, within LRK55_RS09050 at 1985.606 kb on - strand, within LRK55_RS09050 at 1985.701 kb on - strand at 1985.701 kb on - strand at 1985.701 kb on - strand at 1985.701 kb on - strand at 1985.701 kb on - strand at 1985.701 kb on - strand at 1985.701 kb on - strand at 1985.701 kb on - strand at 1985.701 kb on - strand at 1985.701 kb on - strand at 1985.803 kb on + strand at 1985.855 kb on + strand at 1985.855 kb on + strand at 1985.855 kb on + strand at 1985.855 kb on + strand at 1985.855 kb on + strand at 1985.855 kb on + strand at 1985.855 kb on + strand at 1985.856 kb on - strand at 1985.856 kb on - strand at 1985.856 kb on - strand at 1985.856 kb on - strand at 1985.856 kb on - strand at 1985.856 kb on - strand at 1985.856 kb on - strand at 1985.856 kb on - strand at 1985.856 kb on - strand at 1985.891 kb on + strand, within LRK55_RS09055 at 1985.891 kb on + strand, within LRK55_RS09055 at 1985.891 kb on + strand, within LRK55_RS09055 at 1985.891 kb on + strand, within LRK55_RS09055 at 1985.891 kb on + strand, within LRK55_RS09055 at 1985.892 kb on - strand, within LRK55_RS09055 at 1985.892 kb on - strand, within LRK55_RS09055 at 1985.951 kb on + strand, within LRK55_RS09055 at 1985.951 kb on + strand, within LRK55_RS09055 at 1985.952 kb on - strand, within LRK55_RS09055 at 1985.952 kb on - strand, within LRK55_RS09055 at 1985.952 kb on - strand, within LRK55_RS09055 at 1986.077 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 64 uM remove 1,983,206 + LRK55_RS09035 0.82 -1.5 1,983,275 + LRK55_RS09035 0.87 -1.7 1,983,278 + LRK55_RS09035 0.87 -2.5 1,983,278 + LRK55_RS09035 0.87 -1.0 1,983,278 + LRK55_RS09035 0.87 -2.0 1,983,320 + LRK55_RS09035 0.90 -1.5 1,983,511 - -1.2 1,983,519 + -2.5 1,983,519 + +0.8 1,983,520 - +1.1 1,983,520 - -0.1 1,983,520 - -0.1 1,983,520 - -1.2 1,983,520 - -1.8 1,983,520 - +0.2 1,983,544 + +0.4 1,983,544 + -0.5 1,983,545 - -0.2 1,983,545 - -1.8 1,983,573 - -1.0 1,983,666 - -0.2 1,983,683 + +1.0 1,983,684 - +0.5 1,983,970 + LRK55_RS09040 0.75 +0.4 1,983,970 + LRK55_RS09040 0.75 +1.4 1,983,970 + LRK55_RS09040 0.75 +0.3 1,983,970 + LRK55_RS09040 0.75 +0.7 1,983,970 + LRK55_RS09040 0.75 -2.8 1,983,970 + LRK55_RS09040 0.75 -1.0 1,983,971 - LRK55_RS09040 0.75 -2.1 1,984,009 + LRK55_RS09040 0.85 -0.7 1,984,010 - LRK55_RS09040 0.85 +1.3 1,985,201 + -1.7 1,985,378 + +0.9 1,985,378 + +1.1 1,985,379 - +0.3 1,985,379 - +1.6 1,985,606 - LRK55_RS09050 0.58 -1.2 1,985,606 - LRK55_RS09050 0.58 -1.7 1,985,606 - LRK55_RS09050 0.58 -1.0 1,985,701 - -3.9 1,985,701 - -1.7 1,985,701 - -2.5 1,985,701 - -2.9 1,985,701 - -0.5 1,985,701 - -2.0 1,985,701 - -2.0 1,985,701 - -2.0 1,985,701 - -1.1 1,985,701 - -1.2 1,985,803 + -1.7 1,985,855 + +0.1 1,985,855 + -1.0 1,985,855 + -0.4 1,985,855 + -0.7 1,985,855 + +0.5 1,985,855 + -0.2 1,985,855 + -0.3 1,985,856 - -0.5 1,985,856 - -2.0 1,985,856 - -3.4 1,985,856 - +0.7 1,985,856 - -0.8 1,985,856 - -0.1 1,985,856 - +0.6 1,985,856 - -1.8 1,985,856 - +0.7 1,985,891 + LRK55_RS09055 0.20 -0.2 1,985,891 + LRK55_RS09055 0.20 -0.4 1,985,891 + LRK55_RS09055 0.20 -0.2 1,985,891 + LRK55_RS09055 0.20 -0.2 1,985,891 + LRK55_RS09055 0.20 +0.5 1,985,892 - LRK55_RS09055 0.21 +0.0 1,985,892 - LRK55_RS09055 0.21 +0.5 1,985,951 + LRK55_RS09055 0.46 -1.2 1,985,951 + LRK55_RS09055 0.46 -0.6 1,985,952 - LRK55_RS09055 0.46 -0.4 1,985,952 - LRK55_RS09055 0.46 +0.1 1,985,952 - LRK55_RS09055 0.46 -2.9 1,986,077 + -1.2
Or see this region's nucleotide sequence