Strain Fitness in Rhodanobacter denitrificans MT42 around LRK55_RS05730

Experiment: R2A_PIPES with Cadmium chloride 64 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntLRK55_RS05720 and LRK55_RS05725 are separated by 196 nucleotidesLRK55_RS05725 and LRK55_RS05730 are separated by 11 nucleotidesLRK55_RS05730 and LRK55_RS05735 are separated by 18 nucleotides LRK55_RS05720: LRK55_RS05720 - biotin--[acetyl-CoA-carboxylase] ligase, at 1,237,388 to 1,238,377 _RS05720 LRK55_RS05725: LRK55_RS05725 - hypothetical protein, at 1,238,574 to 1,238,897 _RS05725 LRK55_RS05730: LRK55_RS05730 - zinc-dependent peptidase, at 1,238,909 to 1,239,703 _RS05730 LRK55_RS05735: LRK55_RS05735 - ATP-binding protein, at 1,239,722 to 1,241,143 _RS05735 Position (kb) 1238 1239 1240Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1238.459 kb on + strandat 1238.511 kb on + strandat 1238.780 kb on + strand, within LRK55_RS05725at 1238.780 kb on + strand, within LRK55_RS05725at 1238.780 kb on + strand, within LRK55_RS05725at 1238.780 kb on + strand, within LRK55_RS05725at 1239.110 kb on + strand, within LRK55_RS05730at 1239.110 kb on + strand, within LRK55_RS05730at 1239.110 kb on + strand, within LRK55_RS05730at 1239.110 kb on + strand, within LRK55_RS05730at 1239.111 kb on - strand, within LRK55_RS05730at 1239.111 kb on - strand, within LRK55_RS05730at 1239.200 kb on + strand, within LRK55_RS05730at 1239.200 kb on + strand, within LRK55_RS05730at 1239.200 kb on + strand, within LRK55_RS05730at 1239.201 kb on - strand, within LRK55_RS05730at 1239.359 kb on + strand, within LRK55_RS05730at 1239.359 kb on + strand, within LRK55_RS05730at 1239.360 kb on - strand, within LRK55_RS05730at 1239.360 kb on - strand, within LRK55_RS05730at 1239.360 kb on - strand, within LRK55_RS05730at 1239.360 kb on - strand, within LRK55_RS05730at 1239.360 kb on - strand, within LRK55_RS05730at 1239.360 kb on - strand, within LRK55_RS05730at 1239.479 kb on + strand, within LRK55_RS05730at 1239.479 kb on + strand, within LRK55_RS05730at 1239.479 kb on + strand, within LRK55_RS05730at 1239.480 kb on - strand, within LRK55_RS05730at 1239.480 kb on - strand, within LRK55_RS05730at 1239.533 kb on + strand, within LRK55_RS05730at 1239.534 kb on - strand, within LRK55_RS05730at 1239.557 kb on + strand, within LRK55_RS05730at 1239.557 kb on + strand, within LRK55_RS05730at 1239.557 kb on + strand, within LRK55_RS05730at 1239.558 kb on - strand, within LRK55_RS05730at 1239.558 kb on - strand, within LRK55_RS05730at 1239.558 kb on - strand, within LRK55_RS05730at 1239.584 kb on + strand, within LRK55_RS05730at 1239.584 kb on + strand, within LRK55_RS05730at 1239.644 kb on + strandat 1239.644 kb on + strandat 1239.644 kb on + strandat 1239.644 kb on + strandat 1239.644 kb on + strandat 1239.644 kb on + strandat 1239.645 kb on - strandat 1239.645 kb on - strandat 1239.645 kb on - strandat 1239.645 kb on - strandat 1239.645 kb on - strandat 1239.645 kb on - strandat 1239.645 kb on - strandat 1239.645 kb on - strandat 1239.645 kb on - strandat 1239.645 kb on - strandat 1239.647 kb on + strandat 1239.647 kb on + strandat 1239.647 kb on + strandat 1239.648 kb on - strandat 1239.648 kb on - strandat 1239.648 kb on - strandat 1239.648 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 64 uM
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1,238,459 + +0.5
1,238,511 + -1.4
1,238,780 + LRK55_RS05725 0.64 +0.7
1,238,780 + LRK55_RS05725 0.64 +1.4
1,238,780 + LRK55_RS05725 0.64 +0.6
1,238,780 + LRK55_RS05725 0.64 +1.1
1,239,110 + LRK55_RS05730 0.25 -2.9
1,239,110 + LRK55_RS05730 0.25 -0.1
1,239,110 + LRK55_RS05730 0.25 -3.0
1,239,110 + LRK55_RS05730 0.25 -0.2
1,239,111 - LRK55_RS05730 0.25 -0.8
1,239,111 - LRK55_RS05730 0.25 +0.1
1,239,200 + LRK55_RS05730 0.37 +0.5
1,239,200 + LRK55_RS05730 0.37 -1.0
1,239,200 + LRK55_RS05730 0.37 -0.8
1,239,201 - LRK55_RS05730 0.37 +2.5
1,239,359 + LRK55_RS05730 0.57 +1.2
1,239,359 + LRK55_RS05730 0.57 -0.2
1,239,360 - LRK55_RS05730 0.57 -3.9
1,239,360 - LRK55_RS05730 0.57 -2.4
1,239,360 - LRK55_RS05730 0.57 +0.9
1,239,360 - LRK55_RS05730 0.57 -1.9
1,239,360 - LRK55_RS05730 0.57 +0.3
1,239,360 - LRK55_RS05730 0.57 -0.8
1,239,479 + LRK55_RS05730 0.72 -1.3
1,239,479 + LRK55_RS05730 0.72 +0.6
1,239,479 + LRK55_RS05730 0.72 +1.4
1,239,480 - LRK55_RS05730 0.72 +0.0
1,239,480 - LRK55_RS05730 0.72 -0.9
1,239,533 + LRK55_RS05730 0.78 -1.4
1,239,534 - LRK55_RS05730 0.79 -0.4
1,239,557 + LRK55_RS05730 0.82 -0.8
1,239,557 + LRK55_RS05730 0.82 +2.6
1,239,557 + LRK55_RS05730 0.82 -0.3
1,239,558 - LRK55_RS05730 0.82 +1.2
1,239,558 - LRK55_RS05730 0.82 -0.5
1,239,558 - LRK55_RS05730 0.82 +1.2
1,239,584 + LRK55_RS05730 0.85 -0.9
1,239,584 + LRK55_RS05730 0.85 +0.9
1,239,644 + -2.0
1,239,644 + +0.5
1,239,644 + +1.5
1,239,644 + -0.9
1,239,644 + -2.2
1,239,644 + +0.2
1,239,645 - +1.0
1,239,645 - -0.1
1,239,645 - -3.6
1,239,645 - -2.4
1,239,645 - +1.0
1,239,645 - -1.4
1,239,645 - +1.2
1,239,645 - +0.2
1,239,645 - -0.9
1,239,645 - -0.7
1,239,647 + -0.1
1,239,647 + +0.1
1,239,647 + -0.4
1,239,648 - -2.4
1,239,648 - -3.4
1,239,648 - -1.6
1,239,648 - -3.2

Or see this region's nucleotide sequence