Experiment: R2A_PIPES with Cadmium chloride 64 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS00555 and LRK55_RS00560 are separated by 262 nucleotides LRK55_RS00560 and LRK55_RS00565 are separated by 74 nucleotides LRK55_RS00565 and LRK55_RS00570 overlap by 2 nucleotides
LRK55_RS00555: LRK55_RS00555 - LysR family transcriptional regulator, at 123,581 to 124,531
_RS00555
LRK55_RS00560: LRK55_RS00560 - aconitate hydratase AcnA, at 124,794 to 127,535
_RS00560
LRK55_RS00565: LRK55_RS00565 - hypothetical protein, at 127,610 to 128,200
_RS00565
LRK55_RS00570: LRK55_RS00570 - long-chain-fatty-acid--CoA ligase, at 128,199 to 129,884
_RS00570
Position (kb)
124
125
126
127
128 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 124.186 kb on - strand, within LRK55_RS00555 at 124.460 kb on + strand at 124.460 kb on + strand at 124.460 kb on + strand at 124.460 kb on + strand at 124.460 kb on + strand at 124.460 kb on + strand at 124.460 kb on + strand at 124.460 kb on + strand at 124.460 kb on + strand at 124.460 kb on + strand at 124.460 kb on + strand at 124.461 kb on - strand at 124.461 kb on - strand at 124.461 kb on - strand at 124.461 kb on - strand at 124.461 kb on - strand at 124.461 kb on - strand at 124.461 kb on - strand at 124.461 kb on - strand at 124.461 kb on - strand at 124.461 kb on - strand at 124.609 kb on + strand at 124.609 kb on + strand at 124.610 kb on - strand at 124.610 kb on - strand at 124.701 kb on + strand at 124.705 kb on + strand at 124.705 kb on + strand at 124.705 kb on + strand at 124.705 kb on + strand at 124.705 kb on + strand at 124.705 kb on + strand at 124.705 kb on + strand at 124.705 kb on + strand at 124.705 kb on + strand at 124.706 kb on - strand at 127.611 kb on + strand at 127.611 kb on + strand at 127.612 kb on - strand at 127.636 kb on + strand at 127.636 kb on + strand at 127.636 kb on + strand at 127.636 kb on + strand at 127.636 kb on + strand at 127.637 kb on - strand at 127.637 kb on - strand at 127.637 kb on - strand at 127.637 kb on - strand at 127.637 kb on - strand at 127.637 kb on - strand at 127.637 kb on - strand at 127.637 kb on - strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.638 kb on + strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.639 kb on - strand at 127.686 kb on + strand, within LRK55_RS00565 at 127.686 kb on + strand, within LRK55_RS00565 at 127.686 kb on + strand, within LRK55_RS00565 at 127.686 kb on + strand, within LRK55_RS00565 at 127.687 kb on - strand, within LRK55_RS00565 at 127.779 kb on - strand, within LRK55_RS00565 at 127.834 kb on + strand, within LRK55_RS00565 at 127.834 kb on + strand, within LRK55_RS00565 at 127.834 kb on + strand, within LRK55_RS00565 at 127.834 kb on + strand, within LRK55_RS00565 at 127.835 kb on - strand, within LRK55_RS00565 at 127.835 kb on - strand, within LRK55_RS00565 at 127.835 kb on - strand, within LRK55_RS00565 at 127.835 kb on - strand, within LRK55_RS00565 at 127.835 kb on - strand, within LRK55_RS00565 at 127.835 kb on - strand, within LRK55_RS00565 at 128.100 kb on + strand, within LRK55_RS00565 at 128.100 kb on + strand, within LRK55_RS00565 at 128.101 kb on - strand, within LRK55_RS00565 at 128.101 kb on - strand, within LRK55_RS00565 at 128.101 kb on - strand, within LRK55_RS00565 at 128.101 kb on - strand, within LRK55_RS00565 at 128.141 kb on + strand, within LRK55_RS00565 at 128.141 kb on + strand, within LRK55_RS00565 at 128.141 kb on + strand, within LRK55_RS00565 at 128.141 kb on + strand, within LRK55_RS00565 at 128.142 kb on - strand at 128.142 kb on - strand at 128.142 kb on - strand at 128.229 kb on + strand at 128.229 kb on + strand at 128.229 kb on + strand at 128.268 kb on + strand at 128.268 kb on + strand at 128.269 kb on - strand at 128.269 kb on - strand at 128.269 kb on - strand at 128.269 kb on - strand at 128.269 kb on - strand at 128.269 kb on - strand at 128.269 kb on - strand at 128.269 kb on - strand at 128.355 kb on + strand at 128.355 kb on + strand at 128.356 kb on - strand at 128.395 kb on - strand, within LRK55_RS00570 at 128.526 kb on + strand, within LRK55_RS00570 at 128.526 kb on + strand, within LRK55_RS00570 at 128.526 kb on + strand, within LRK55_RS00570 at 128.527 kb on - strand, within LRK55_RS00570 at 128.527 kb on - strand, within LRK55_RS00570 at 128.527 kb on - strand, within LRK55_RS00570
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Cadmium chloride 64 uM remove 124,186 - LRK55_RS00555 0.64 +1.8 124,460 + +0.1 124,460 + +0.2 124,460 + -1.9 124,460 + -1.0 124,460 + +2.2 124,460 + +0.2 124,460 + -1.3 124,460 + -1.8 124,460 + +0.8 124,460 + +1.4 124,460 + -0.0 124,461 - -0.2 124,461 - +1.4 124,461 - -0.5 124,461 - +0.3 124,461 - -1.7 124,461 - -2.4 124,461 - +0.1 124,461 - +1.8 124,461 - +0.4 124,461 - +2.0 124,609 + +0.7 124,609 + +1.0 124,610 - -1.3 124,610 - +1.2 124,701 + +0.0 124,705 + +1.2 124,705 + +1.1 124,705 + +0.5 124,705 + -1.0 124,705 + -0.8 124,705 + +0.6 124,705 + -0.9 124,705 + +0.4 124,705 + -1.3 124,706 - -0.8 127,611 + +1.0 127,611 + -2.3 127,612 - -1.6 127,636 + +1.8 127,636 + -0.2 127,636 + -1.3 127,636 + +1.0 127,636 + -2.3 127,637 - +1.2 127,637 - -0.8 127,637 - -2.8 127,637 - +0.2 127,637 - +1.0 127,637 - -0.8 127,637 - -0.8 127,637 - +0.4 127,638 + -1.3 127,638 + +2.0 127,638 + -0.4 127,638 + +1.2 127,638 + +0.2 127,638 + +0.2 127,638 + -2.3 127,638 + -0.3 127,638 + -0.9 127,638 + +1.3 127,638 + -0.3 127,638 + -1.2 127,638 + +1.2 127,638 + -2.1 127,638 + -0.1 127,638 + -3.1 127,638 + -1.7 127,638 + +0.6 127,638 + +0.4 127,638 + +1.2 127,638 + +0.0 127,638 + +1.3 127,638 + -2.2 127,638 + -0.1 127,638 + -0.8 127,638 + +1.3 127,638 + -1.1 127,639 - +0.0 127,639 - -0.1 127,639 - -0.3 127,639 - +1.2 127,639 - -0.6 127,639 - -0.1 127,639 - +0.3 127,639 - +1.2 127,639 - -1.9 127,639 - +1.6 127,639 - +2.9 127,639 - -0.3 127,639 - -2.3 127,639 - -1.6 127,639 - +0.2 127,639 - +1.2 127,639 - -1.8 127,639 - +0.8 127,639 - -2.1 127,639 - -1.1 127,639 - +1.2 127,639 - -0.3 127,639 - -3.1 127,639 - -0.0 127,639 - +1.6 127,639 - -1.2 127,639 - +0.2 127,639 - -0.1 127,639 - +0.2 127,639 - -1.4 127,639 - +0.8 127,639 - +0.6 127,639 - -1.3 127,686 + LRK55_RS00565 0.13 +2.2 127,686 + LRK55_RS00565 0.13 -1.9 127,686 + LRK55_RS00565 0.13 +1.5 127,686 + LRK55_RS00565 0.13 +0.2 127,687 - LRK55_RS00565 0.13 +0.4 127,779 - LRK55_RS00565 0.29 -1.7 127,834 + LRK55_RS00565 0.38 +0.2 127,834 + LRK55_RS00565 0.38 -1.7 127,834 + LRK55_RS00565 0.38 -2.8 127,834 + LRK55_RS00565 0.38 -1.3 127,835 - LRK55_RS00565 0.38 -1.4 127,835 - LRK55_RS00565 0.38 +0.9 127,835 - LRK55_RS00565 0.38 +1.8 127,835 - LRK55_RS00565 0.38 +2.7 127,835 - LRK55_RS00565 0.38 -3.5 127,835 - LRK55_RS00565 0.38 -0.3 128,100 + LRK55_RS00565 0.83 -1.1 128,100 + LRK55_RS00565 0.83 -1.3 128,101 - LRK55_RS00565 0.83 +0.6 128,101 - LRK55_RS00565 0.83 -0.3 128,101 - LRK55_RS00565 0.83 -0.1 128,101 - LRK55_RS00565 0.83 +0.3 128,141 + LRK55_RS00565 0.90 +1.2 128,141 + LRK55_RS00565 0.90 -3.1 128,141 + LRK55_RS00565 0.90 +0.4 128,141 + LRK55_RS00565 0.90 -3.0 128,142 - -1.1 128,142 - +1.4 128,142 - +0.2 128,229 + +3.2 128,229 + -0.3 128,229 + -0.8 128,268 + -2.5 128,268 + -0.8 128,269 - -0.3 128,269 - -2.2 128,269 - -0.3 128,269 - -1.9 128,269 - +2.0 128,269 - -1.7 128,269 - -2.7 128,269 - -1.8 128,355 + -1.6 128,355 + -0.0 128,356 - -0.6 128,395 - LRK55_RS00570 0.12 +0.8 128,526 + LRK55_RS00570 0.19 -1.3 128,526 + LRK55_RS00570 0.19 -2.1 128,526 + LRK55_RS00570 0.19 +0.7 128,527 - LRK55_RS00570 0.19 +0.6 128,527 - LRK55_RS00570 0.19 -0.2 128,527 - LRK55_RS00570 0.19 -0.9
Or see this region's nucleotide sequence