Experiment: mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01614 and MPMX20_01615 are separated by 25 nucleotides MPMX20_01615 and MPMX20_01616 are separated by 34 nucleotides MPMX20_01616 and MPMX20_01617 are separated by 60 nucleotides
MPMX20_01614: MPMX20_01614 - hypothetical protein, at 1,707,451 to 1,708,260
_01614
MPMX20_01615: MPMX20_01615 - putative protein YccU, at 1,708,286 to 1,708,702
_01615
MPMX20_01616: MPMX20_01616 - Heat shock protein HspQ, at 1,708,737 to 1,709,054
_01616
MPMX20_01617: MPMX20_01617 - Ribosomal RNA large subunit methyltransferase I, at 1,709,115 to 1,710,305
_01617
Position (kb)
1708
1709
1710 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1707.770 kb on - strand, within MPMX20_01614 at 1707.779 kb on + strand, within MPMX20_01614 at 1707.827 kb on + strand, within MPMX20_01614 at 1707.827 kb on + strand, within MPMX20_01614 at 1707.835 kb on - strand, within MPMX20_01614 at 1707.851 kb on + strand, within MPMX20_01614 at 1707.878 kb on + strand, within MPMX20_01614 at 1707.902 kb on - strand, within MPMX20_01614 at 1707.951 kb on - strand, within MPMX20_01614 at 1708.041 kb on + strand, within MPMX20_01614 at 1708.191 kb on - strand at 1708.210 kb on - strand at 1708.255 kb on - strand at 1708.302 kb on - strand at 1708.347 kb on - strand, within MPMX20_01615 at 1708.379 kb on - strand, within MPMX20_01615 at 1708.390 kb on - strand, within MPMX20_01615 at 1708.419 kb on - strand, within MPMX20_01615 at 1708.419 kb on - strand, within MPMX20_01615 at 1708.462 kb on + strand, within MPMX20_01615 at 1708.462 kb on + strand, within MPMX20_01615 at 1708.462 kb on + strand, within MPMX20_01615 at 1708.462 kb on + strand, within MPMX20_01615 at 1708.470 kb on - strand, within MPMX20_01615 at 1708.604 kb on - strand, within MPMX20_01615 at 1708.643 kb on - strand, within MPMX20_01615 at 1708.680 kb on + strand at 1708.766 kb on - strand at 1708.841 kb on - strand, within MPMX20_01616 at 1708.860 kb on - strand, within MPMX20_01616 at 1709.083 kb on + strand at 1709.106 kb on - strand at 1709.162 kb on + strand at 1709.186 kb on - strand at 1709.209 kb on - strand at 1709.279 kb on + strand, within MPMX20_01617 at 1709.295 kb on + strand, within MPMX20_01617 at 1709.303 kb on - strand, within MPMX20_01617 at 1709.303 kb on - strand, within MPMX20_01617 at 1709.322 kb on - strand, within MPMX20_01617 at 1709.348 kb on + strand, within MPMX20_01617 at 1709.365 kb on + strand, within MPMX20_01617 at 1709.506 kb on - strand, within MPMX20_01617 at 1709.570 kb on + strand, within MPMX20_01617 at 1709.578 kb on - strand, within MPMX20_01617 at 1709.581 kb on - strand, within MPMX20_01617 at 1709.655 kb on - strand, within MPMX20_01617 at 1709.684 kb on + strand, within MPMX20_01617 at 1709.684 kb on + strand, within MPMX20_01617 at 1709.684 kb on + strand, within MPMX20_01617 at 1709.684 kb on + strand, within MPMX20_01617 at 1709.684 kb on + strand, within MPMX20_01617 at 1709.692 kb on - strand, within MPMX20_01617 at 1709.692 kb on - strand, within MPMX20_01617 at 1709.732 kb on + strand, within MPMX20_01617 at 1709.732 kb on + strand, within MPMX20_01617 at 1709.732 kb on + strand, within MPMX20_01617 at 1709.732 kb on + strand, within MPMX20_01617 at 1709.732 kb on + strand, within MPMX20_01617 at 1709.740 kb on - strand, within MPMX20_01617 at 1709.740 kb on - strand, within MPMX20_01617 at 1709.740 kb on - strand, within MPMX20_01617 at 1709.740 kb on - strand, within MPMX20_01617 at 1709.774 kb on + strand, within MPMX20_01617 at 1709.774 kb on + strand, within MPMX20_01617 at 1709.878 kb on + strand, within MPMX20_01617 at 1709.883 kb on + strand, within MPMX20_01617 at 1709.883 kb on + strand, within MPMX20_01617 at 1709.886 kb on - strand, within MPMX20_01617 at 1709.891 kb on - strand, within MPMX20_01617 at 1709.891 kb on - strand, within MPMX20_01617 at 1709.896 kb on - strand, within MPMX20_01617 at 1709.903 kb on + strand, within MPMX20_01617 at 1709.914 kb on + strand, within MPMX20_01617 at 1709.932 kb on + strand, within MPMX20_01617 at 1710.052 kb on - strand, within MPMX20_01617
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A remove 1,707,770 - MPMX20_01614 0.39 +0.5 1,707,779 + MPMX20_01614 0.40 +0.4 1,707,827 + MPMX20_01614 0.46 +1.3 1,707,827 + MPMX20_01614 0.46 +0.1 1,707,835 - MPMX20_01614 0.47 -0.6 1,707,851 + MPMX20_01614 0.49 +0.4 1,707,878 + MPMX20_01614 0.53 -0.2 1,707,902 - MPMX20_01614 0.56 -2.5 1,707,951 - MPMX20_01614 0.62 +0.5 1,708,041 + MPMX20_01614 0.73 -0.0 1,708,191 - +0.4 1,708,210 - -0.2 1,708,255 - -1.4 1,708,302 - +1.6 1,708,347 - MPMX20_01615 0.15 -0.4 1,708,379 - MPMX20_01615 0.22 +0.8 1,708,390 - MPMX20_01615 0.25 -0.9 1,708,419 - MPMX20_01615 0.32 -0.1 1,708,419 - MPMX20_01615 0.32 -0.2 1,708,462 + MPMX20_01615 0.42 +0.2 1,708,462 + MPMX20_01615 0.42 +0.3 1,708,462 + MPMX20_01615 0.42 +0.3 1,708,462 + MPMX20_01615 0.42 -1.1 1,708,470 - MPMX20_01615 0.44 -1.0 1,708,604 - MPMX20_01615 0.76 +0.1 1,708,643 - MPMX20_01615 0.86 -1.3 1,708,680 + +0.5 1,708,766 - -0.0 1,708,841 - MPMX20_01616 0.33 -2.2 1,708,860 - MPMX20_01616 0.39 +1.8 1,709,083 + +0.1 1,709,106 - -0.4 1,709,162 + +0.3 1,709,186 - -0.0 1,709,209 - -0.0 1,709,279 + MPMX20_01617 0.14 +0.4 1,709,295 + MPMX20_01617 0.15 -0.1 1,709,303 - MPMX20_01617 0.16 +0.7 1,709,303 - MPMX20_01617 0.16 -0.1 1,709,322 - MPMX20_01617 0.17 -0.8 1,709,348 + MPMX20_01617 0.20 -0.4 1,709,365 + MPMX20_01617 0.21 -0.4 1,709,506 - MPMX20_01617 0.33 -1.4 1,709,570 + MPMX20_01617 0.38 -0.4 1,709,578 - MPMX20_01617 0.39 +1.6 1,709,581 - MPMX20_01617 0.39 +0.9 1,709,655 - MPMX20_01617 0.45 -0.1 1,709,684 + MPMX20_01617 0.48 -0.4 1,709,684 + MPMX20_01617 0.48 -0.6 1,709,684 + MPMX20_01617 0.48 -0.7 1,709,684 + MPMX20_01617 0.48 -0.2 1,709,684 + MPMX20_01617 0.48 +0.2 1,709,692 - MPMX20_01617 0.48 -0.2 1,709,692 - MPMX20_01617 0.48 +0.0 1,709,732 + MPMX20_01617 0.52 -0.5 1,709,732 + MPMX20_01617 0.52 -0.1 1,709,732 + MPMX20_01617 0.52 +0.5 1,709,732 + MPMX20_01617 0.52 -2.0 1,709,732 + MPMX20_01617 0.52 -2.3 1,709,740 - MPMX20_01617 0.52 -0.6 1,709,740 - MPMX20_01617 0.52 -0.0 1,709,740 - MPMX20_01617 0.52 -0.1 1,709,740 - MPMX20_01617 0.52 +0.8 1,709,774 + MPMX20_01617 0.55 +0.1 1,709,774 + MPMX20_01617 0.55 +0.1 1,709,878 + MPMX20_01617 0.64 +0.3 1,709,883 + MPMX20_01617 0.64 +0.3 1,709,883 + MPMX20_01617 0.64 -0.6 1,709,886 - MPMX20_01617 0.65 -0.8 1,709,891 - MPMX20_01617 0.65 +0.5 1,709,891 - MPMX20_01617 0.65 +0.3 1,709,896 - MPMX20_01617 0.66 +0.5 1,709,903 + MPMX20_01617 0.66 -0.4 1,709,914 + MPMX20_01617 0.67 -0.1 1,709,932 + MPMX20_01617 0.69 -0.6 1,710,052 - MPMX20_01617 0.79 -0.2
Or see this region's nucleotide sequence