Experiment: mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00827 and MPMX20_00828 are separated by 122 nucleotides MPMX20_00828 and MPMX20_00829 overlap by 4 nucleotides MPMX20_00829 and MPMX20_00830 are separated by 96 nucleotides
MPMX20_00827: MPMX20_00827 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 869,351 to 870,142
_00827
MPMX20_00828: MPMX20_00828 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 870,265 to 870,744
_00828
MPMX20_00829: MPMX20_00829 - Poly(A) polymerase I, at 870,741 to 872,084
_00829
MPMX20_00830: MPMX20_00830 - Glutamyl-Q tRNA(Asp) synthetase, at 872,181 to 873,071
_00830
Position (kb) 870
871
872
873 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 869.742 kb on - strand, within MPMX20_00827 at 869.858 kb on - strand, within MPMX20_00827 at 869.917 kb on - strand, within MPMX20_00827 at 870.027 kb on + strand, within MPMX20_00827 at 870.027 kb on - strand, within MPMX20_00827 at 870.027 kb on - strand, within MPMX20_00827 at 870.027 kb on - strand, within MPMX20_00827 at 870.027 kb on - strand, within MPMX20_00827 at 870.033 kb on - strand, within MPMX20_00827 at 870.035 kb on - strand, within MPMX20_00827 at 870.035 kb on - strand, within MPMX20_00827 at 870.067 kb on - strand at 870.067 kb on - strand at 870.081 kb on - strand at 870.093 kb on + strand at 870.130 kb on - strand at 870.192 kb on + strand at 870.200 kb on - strand at 870.250 kb on - strand at 870.250 kb on - strand at 870.866 kb on + strand at 870.941 kb on - strand, within MPMX20_00829 at 870.941 kb on - strand, within MPMX20_00829 at 870.968 kb on + strand, within MPMX20_00829 at 871.114 kb on + strand, within MPMX20_00829 at 871.153 kb on + strand, within MPMX20_00829 at 871.247 kb on - strand, within MPMX20_00829 at 871.354 kb on - strand, within MPMX20_00829 at 871.372 kb on + strand, within MPMX20_00829 at 871.383 kb on + strand, within MPMX20_00829 at 871.420 kb on + strand, within MPMX20_00829 at 871.428 kb on - strand, within MPMX20_00829 at 871.428 kb on - strand, within MPMX20_00829 at 871.428 kb on - strand, within MPMX20_00829 at 871.428 kb on - strand, within MPMX20_00829 at 871.557 kb on - strand, within MPMX20_00829 at 871.672 kb on - strand, within MPMX20_00829 at 871.732 kb on - strand, within MPMX20_00829 at 871.801 kb on + strand, within MPMX20_00829 at 871.876 kb on - strand, within MPMX20_00829 at 871.971 kb on + strand at 871.988 kb on - strand at 872.023 kb on + strand at 872.097 kb on + strand at 872.097 kb on + strand at 872.105 kb on - strand at 872.105 kb on - strand at 872.138 kb on - strand at 872.187 kb on - strand at 872.328 kb on + strand, within MPMX20_00830 at 872.336 kb on - strand, within MPMX20_00830 at 872.356 kb on + strand, within MPMX20_00830 at 872.395 kb on - strand, within MPMX20_00830 at 872.402 kb on - strand, within MPMX20_00830 at 872.492 kb on + strand, within MPMX20_00830 at 872.492 kb on + strand, within MPMX20_00830 at 872.492 kb on + strand, within MPMX20_00830 at 872.500 kb on - strand, within MPMX20_00830 at 872.500 kb on - strand, within MPMX20_00830 at 872.515 kb on - strand, within MPMX20_00830 at 872.560 kb on - strand, within MPMX20_00830 at 872.690 kb on + strand, within MPMX20_00830 at 872.690 kb on + strand, within MPMX20_00830 at 872.693 kb on - strand, within MPMX20_00830 at 872.693 kb on - strand, within MPMX20_00830 at 872.698 kb on - strand, within MPMX20_00830 at 872.698 kb on - strand, within MPMX20_00830 at 872.698 kb on - strand, within MPMX20_00830 at 872.734 kb on - strand, within MPMX20_00830 at 872.760 kb on + strand, within MPMX20_00830 at 872.769 kb on - strand, within MPMX20_00830 at 872.895 kb on + strand, within MPMX20_00830 at 872.900 kb on + strand, within MPMX20_00830 at 872.974 kb on - strand, within MPMX20_00830 at 873.047 kb on + strand at 873.069 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A remove 869,742 - MPMX20_00827 0.49 -1.1 869,858 - MPMX20_00827 0.64 -1.5 869,917 - MPMX20_00827 0.71 +0.1 870,027 + MPMX20_00827 0.85 -0.1 870,027 - MPMX20_00827 0.85 -0.7 870,027 - MPMX20_00827 0.85 -0.2 870,027 - MPMX20_00827 0.85 -0.3 870,027 - MPMX20_00827 0.85 -0.9 870,033 - MPMX20_00827 0.86 -0.3 870,035 - MPMX20_00827 0.86 -0.7 870,035 - MPMX20_00827 0.86 -0.3 870,067 - -1.1 870,067 - -0.8 870,081 - -2.8 870,093 + +2.5 870,130 - -0.2 870,192 + +0.2 870,200 - +0.3 870,250 - -0.0 870,250 - -3.0 870,866 + -0.5 870,941 - MPMX20_00829 0.15 +1.1 870,941 - MPMX20_00829 0.15 -0.7 870,968 + MPMX20_00829 0.17 +0.0 871,114 + MPMX20_00829 0.28 -0.4 871,153 + MPMX20_00829 0.31 -0.6 871,247 - MPMX20_00829 0.38 -1.3 871,354 - MPMX20_00829 0.46 +0.7 871,372 + MPMX20_00829 0.47 -1.2 871,383 + MPMX20_00829 0.48 -0.4 871,420 + MPMX20_00829 0.51 -1.3 871,428 - MPMX20_00829 0.51 -0.8 871,428 - MPMX20_00829 0.51 +0.2 871,428 - MPMX20_00829 0.51 -2.1 871,428 - MPMX20_00829 0.51 -0.4 871,557 - MPMX20_00829 0.61 -0.6 871,672 - MPMX20_00829 0.69 -0.5 871,732 - MPMX20_00829 0.74 -2.0 871,801 + MPMX20_00829 0.79 +0.3 871,876 - MPMX20_00829 0.84 -1.0 871,971 + -0.4 871,988 - -0.8 872,023 + -0.7 872,097 + -1.4 872,097 + -1.9 872,105 - -1.3 872,105 - +0.2 872,138 - -1.5 872,187 - +0.2 872,328 + MPMX20_00830 0.16 -0.4 872,336 - MPMX20_00830 0.17 +1.6 872,356 + MPMX20_00830 0.20 +0.6 872,395 - MPMX20_00830 0.24 +0.6 872,402 - MPMX20_00830 0.25 -0.0 872,492 + MPMX20_00830 0.35 +0.2 872,492 + MPMX20_00830 0.35 +1.0 872,492 + MPMX20_00830 0.35 -1.6 872,500 - MPMX20_00830 0.36 -1.3 872,500 - MPMX20_00830 0.36 -0.7 872,515 - MPMX20_00830 0.37 +0.1 872,560 - MPMX20_00830 0.43 -0.1 872,690 + MPMX20_00830 0.57 +0.4 872,690 + MPMX20_00830 0.57 -1.5 872,693 - MPMX20_00830 0.57 +0.9 872,693 - MPMX20_00830 0.57 +0.8 872,698 - MPMX20_00830 0.58 +0.2 872,698 - MPMX20_00830 0.58 -0.7 872,698 - MPMX20_00830 0.58 +3.2 872,734 - MPMX20_00830 0.62 +0.0 872,760 + MPMX20_00830 0.65 +0.1 872,769 - MPMX20_00830 0.66 -0.4 872,895 + MPMX20_00830 0.80 +0.3 872,900 + MPMX20_00830 0.81 +0.9 872,974 - MPMX20_00830 0.89 +0.2 873,047 + -0.9 873,069 + -1.0
Or see this region's nucleotide sequence