Experiment: mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00743 and MPMX20_00744 are separated by 114 nucleotides MPMX20_00744 and MPMX20_00745 are separated by 39 nucleotides MPMX20_00745 and MPMX20_00746 are separated by 4 nucleotides MPMX20_00746 and MPMX20_00747 are separated by 3 nucleotides
MPMX20_00743: MPMX20_00743 - Glutathione-regulated potassium-efflux system protein KefC, at 768,537 to 770,402
_00743
MPMX20_00744: MPMX20_00744 - IS1595 family transposase ISSsu9, at 770,517 to 770,996
_00744
MPMX20_00745: MPMX20_00745 - Bis(5'-nucleosyl)-tetraphosphatase, symmetrical, at 771,036 to 771,884
_00745
MPMX20_00746: MPMX20_00746 - Protein ApaG, at 771,889 to 772,266
_00746
MPMX20_00747: MPMX20_00747 - Ribosomal RNA small subunit methyltransferase A, at 772,270 to 773,091
_00747
Position (kb) 771
772 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 770.067 kb on - strand, within MPMX20_00743 at 770.073 kb on - strand, within MPMX20_00743 at 770.098 kb on - strand, within MPMX20_00743 at 770.102 kb on + strand, within MPMX20_00743 at 770.199 kb on + strand, within MPMX20_00743 at 770.215 kb on - strand, within MPMX20_00743 at 770.319 kb on - strand at 770.346 kb on - strand at 770.456 kb on - strand at 771.024 kb on + strand at 771.123 kb on + strand, within MPMX20_00745 at 771.123 kb on + strand, within MPMX20_00745 at 771.123 kb on + strand, within MPMX20_00745 at 771.131 kb on - strand, within MPMX20_00745 at 771.131 kb on - strand, within MPMX20_00745 at 771.131 kb on - strand, within MPMX20_00745 at 771.178 kb on - strand, within MPMX20_00745 at 771.234 kb on - strand, within MPMX20_00745 at 771.372 kb on + strand, within MPMX20_00745 at 771.380 kb on - strand, within MPMX20_00745 at 771.471 kb on - strand, within MPMX20_00745 at 771.596 kb on - strand, within MPMX20_00745 at 771.622 kb on + strand, within MPMX20_00745 at 771.693 kb on + strand, within MPMX20_00745 at 771.701 kb on - strand, within MPMX20_00745 at 771.701 kb on - strand, within MPMX20_00745 at 771.720 kb on - strand, within MPMX20_00745 at 771.814 kb on - strand at 771.821 kb on + strand at 771.842 kb on - strand at 771.870 kb on - strand at 771.976 kb on + strand, within MPMX20_00746 at 771.994 kb on - strand, within MPMX20_00746 at 771.994 kb on - strand, within MPMX20_00746 at 771.994 kb on - strand, within MPMX20_00746 at 772.046 kb on + strand, within MPMX20_00746 at 772.132 kb on + strand, within MPMX20_00746 at 772.268 kb on + strand at 772.276 kb on - strand at 772.321 kb on + strand at 772.329 kb on - strand at 772.329 kb on - strand at 772.442 kb on - strand, within MPMX20_00747 at 772.442 kb on - strand, within MPMX20_00747 at 772.459 kb on - strand, within MPMX20_00747 at 772.473 kb on + strand, within MPMX20_00747 at 772.506 kb on + strand, within MPMX20_00747 at 772.506 kb on + strand, within MPMX20_00747 at 772.526 kb on - strand, within MPMX20_00747 at 772.528 kb on + strand, within MPMX20_00747 at 772.553 kb on - strand, within MPMX20_00747 at 772.573 kb on - strand, within MPMX20_00747 at 772.626 kb on - strand, within MPMX20_00747 at 772.626 kb on - strand, within MPMX20_00747 at 772.724 kb on - strand, within MPMX20_00747 at 772.877 kb on - strand, within MPMX20_00747
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A remove 770,067 - MPMX20_00743 0.82 -0.4 770,073 - MPMX20_00743 0.82 -0.1 770,098 - MPMX20_00743 0.84 -0.1 770,102 + MPMX20_00743 0.84 +0.4 770,199 + MPMX20_00743 0.89 +0.3 770,215 - MPMX20_00743 0.90 -0.6 770,319 - +0.9 770,346 - -0.2 770,456 - -2.3 771,024 + -1.3 771,123 + MPMX20_00745 0.10 -0.7 771,123 + MPMX20_00745 0.10 +0.6 771,123 + MPMX20_00745 0.10 -0.4 771,131 - MPMX20_00745 0.11 +0.7 771,131 - MPMX20_00745 0.11 -0.9 771,131 - MPMX20_00745 0.11 -1.1 771,178 - MPMX20_00745 0.17 -1.3 771,234 - MPMX20_00745 0.23 -2.5 771,372 + MPMX20_00745 0.40 +0.2 771,380 - MPMX20_00745 0.41 -2.0 771,471 - MPMX20_00745 0.51 +0.7 771,596 - MPMX20_00745 0.66 -1.4 771,622 + MPMX20_00745 0.69 -0.1 771,693 + MPMX20_00745 0.77 -2.6 771,701 - MPMX20_00745 0.78 -0.6 771,701 - MPMX20_00745 0.78 -1.7 771,720 - MPMX20_00745 0.81 +0.7 771,814 - -2.3 771,821 + -1.3 771,842 - +0.4 771,870 - +0.3 771,976 + MPMX20_00746 0.23 -0.4 771,994 - MPMX20_00746 0.28 +0.2 771,994 - MPMX20_00746 0.28 -0.3 771,994 - MPMX20_00746 0.28 +4.2 772,046 + MPMX20_00746 0.42 +1.1 772,132 + MPMX20_00746 0.64 +0.1 772,268 + +0.1 772,276 - +1.0 772,321 + +0.9 772,329 - -0.2 772,329 - +1.9 772,442 - MPMX20_00747 0.21 +1.1 772,442 - MPMX20_00747 0.21 +2.1 772,459 - MPMX20_00747 0.23 -2.4 772,473 + MPMX20_00747 0.25 -1.5 772,506 + MPMX20_00747 0.29 +0.1 772,506 + MPMX20_00747 0.29 -0.8 772,526 - MPMX20_00747 0.31 +1.2 772,528 + MPMX20_00747 0.31 -0.1 772,553 - MPMX20_00747 0.34 -0.4 772,573 - MPMX20_00747 0.37 +0.6 772,626 - MPMX20_00747 0.43 -0.6 772,626 - MPMX20_00747 0.43 -0.1 772,724 - MPMX20_00747 0.55 +0.9 772,877 - MPMX20_00747 0.74 -1.2
Or see this region's nucleotide sequence