Experiment: mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00710 and MPMX20_00711 are separated by 1 nucleotides MPMX20_00711 and MPMX20_00712 are separated by 3 nucleotides MPMX20_00712 and MPMX20_00713 are separated by 211 nucleotides MPMX20_00713 and MPMX20_00714 are separated by 34 nucleotides
MPMX20_00710: MPMX20_00710 - Bifunctional aspartokinase/homoserine dehydrogenase 1, at 731,140 to 733,602
_00710
MPMX20_00711: MPMX20_00711 - Homoserine kinase, at 733,604 to 734,533
_00711
MPMX20_00712: MPMX20_00712 - Threonine synthase, at 734,537 to 735,823
_00712
MPMX20_00713: MPMX20_00713 - hypothetical protein, at 736,035 to 736,334
_00713
MPMX20_00714: MPMX20_00714 - Peroxide stress resistance protein YaaA, at 736,369 to 737,139
_00714
Position (kb) 734
735
736 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 733.597 kb on + strand at 733.624 kb on + strand at 733.660 kb on + strand at 733.697 kb on - strand, within MPMX20_00711 at 733.702 kb on - strand, within MPMX20_00711 at 733.731 kb on + strand, within MPMX20_00711 at 733.732 kb on - strand, within MPMX20_00711 at 733.732 kb on - strand, within MPMX20_00711 at 733.745 kb on + strand, within MPMX20_00711 at 733.811 kb on + strand, within MPMX20_00711 at 733.814 kb on - strand, within MPMX20_00711 at 733.814 kb on - strand, within MPMX20_00711 at 733.829 kb on - strand, within MPMX20_00711 at 733.829 kb on - strand, within MPMX20_00711 at 733.881 kb on + strand, within MPMX20_00711 at 733.881 kb on + strand, within MPMX20_00711 at 733.884 kb on - strand, within MPMX20_00711 at 733.886 kb on + strand, within MPMX20_00711 at 733.886 kb on + strand, within MPMX20_00711 at 733.886 kb on + strand, within MPMX20_00711 at 733.889 kb on - strand, within MPMX20_00711 at 733.889 kb on - strand, within MPMX20_00711 at 733.894 kb on - strand, within MPMX20_00711 at 733.997 kb on - strand, within MPMX20_00711 at 734.103 kb on + strand, within MPMX20_00711 at 734.103 kb on + strand, within MPMX20_00711 at 734.103 kb on + strand, within MPMX20_00711 at 734.104 kb on - strand, within MPMX20_00711 at 734.111 kb on - strand, within MPMX20_00711 at 734.111 kb on - strand, within MPMX20_00711 at 734.111 kb on - strand, within MPMX20_00711 at 734.131 kb on - strand, within MPMX20_00711 at 734.139 kb on + strand, within MPMX20_00711 at 734.154 kb on - strand, within MPMX20_00711 at 734.166 kb on + strand, within MPMX20_00711 at 734.197 kb on + strand, within MPMX20_00711 at 734.205 kb on - strand, within MPMX20_00711 at 734.205 kb on - strand, within MPMX20_00711 at 734.214 kb on + strand, within MPMX20_00711 at 734.234 kb on + strand, within MPMX20_00711 at 734.242 kb on - strand, within MPMX20_00711 at 734.302 kb on + strand, within MPMX20_00711 at 734.302 kb on + strand, within MPMX20_00711 at 734.307 kb on + strand, within MPMX20_00711 at 734.307 kb on + strand, within MPMX20_00711 at 734.307 kb on + strand, within MPMX20_00711 at 734.307 kb on + strand, within MPMX20_00711 at 734.315 kb on - strand, within MPMX20_00711 at 734.315 kb on - strand, within MPMX20_00711 at 734.325 kb on + strand, within MPMX20_00711 at 734.368 kb on + strand, within MPMX20_00711 at 734.369 kb on - strand, within MPMX20_00711 at 734.419 kb on + strand, within MPMX20_00711 at 734.452 kb on - strand at 734.503 kb on + strand at 734.527 kb on - strand at 734.572 kb on + strand at 734.572 kb on + strand at 734.587 kb on + strand at 734.595 kb on + strand at 734.595 kb on + strand at 734.603 kb on - strand at 734.603 kb on - strand at 734.623 kb on + strand at 734.661 kb on + strand at 734.672 kb on - strand, within MPMX20_00712 at 734.680 kb on - strand, within MPMX20_00712 at 734.717 kb on + strand, within MPMX20_00712 at 734.808 kb on - strand, within MPMX20_00712 at 734.820 kb on + strand, within MPMX20_00712 at 734.820 kb on + strand, within MPMX20_00712 at 734.820 kb on + strand, within MPMX20_00712 at 734.820 kb on + strand, within MPMX20_00712 at 734.828 kb on - strand, within MPMX20_00712 at 734.830 kb on + strand, within MPMX20_00712 at 734.830 kb on + strand, within MPMX20_00712 at 734.830 kb on + strand, within MPMX20_00712 at 734.843 kb on + strand, within MPMX20_00712 at 734.846 kb on - strand, within MPMX20_00712 at 735.014 kb on + strand, within MPMX20_00712 at 735.155 kb on + strand, within MPMX20_00712 at 735.163 kb on - strand, within MPMX20_00712 at 735.163 kb on - strand, within MPMX20_00712 at 735.202 kb on + strand, within MPMX20_00712 at 735.391 kb on + strand, within MPMX20_00712 at 735.441 kb on + strand, within MPMX20_00712 at 735.493 kb on + strand, within MPMX20_00712 at 735.514 kb on + strand, within MPMX20_00712 at 735.514 kb on + strand, within MPMX20_00712 at 735.541 kb on - strand, within MPMX20_00712 at 735.570 kb on - strand, within MPMX20_00712 at 735.572 kb on + strand, within MPMX20_00712 at 735.582 kb on + strand, within MPMX20_00712 at 735.582 kb on + strand, within MPMX20_00712 at 735.582 kb on + strand, within MPMX20_00712 at 735.582 kb on + strand, within MPMX20_00712 at 735.582 kb on + strand, within MPMX20_00712 at 735.582 kb on + strand, within MPMX20_00712 at 735.582 kb on + strand, within MPMX20_00712 at 735.582 kb on + strand, within MPMX20_00712 at 735.583 kb on - strand, within MPMX20_00712 at 735.583 kb on - strand, within MPMX20_00712 at 735.590 kb on - strand, within MPMX20_00712 at 735.590 kb on - strand, within MPMX20_00712 at 735.590 kb on - strand, within MPMX20_00712 at 735.590 kb on - strand, within MPMX20_00712 at 735.617 kb on - strand, within MPMX20_00712 at 735.617 kb on - strand, within MPMX20_00712 at 735.650 kb on - strand, within MPMX20_00712 at 735.652 kb on + strand, within MPMX20_00712 at 735.734 kb on - strand at 735.834 kb on + strand at 735.834 kb on + strand at 735.848 kb on + strand at 735.968 kb on - strand at 736.007 kb on + strand at 736.103 kb on - strand, within MPMX20_00713 at 736.103 kb on - strand, within MPMX20_00713 at 736.151 kb on + strand, within MPMX20_00713 at 736.220 kb on - strand, within MPMX20_00713 at 736.272 kb on - strand, within MPMX20_00713 at 736.381 kb on + strand at 736.444 kb on - strand at 736.446 kb on - strand, within MPMX20_00714 at 736.474 kb on + strand, within MPMX20_00714 at 736.474 kb on + strand, within MPMX20_00714 at 736.482 kb on - strand, within MPMX20_00714 at 736.532 kb on + strand, within MPMX20_00714 at 736.532 kb on + strand, within MPMX20_00714 at 736.532 kb on + strand, within MPMX20_00714 at 736.532 kb on + strand, within MPMX20_00714 at 736.540 kb on - strand, within MPMX20_00714 at 736.540 kb on - strand, within MPMX20_00714 at 736.540 kb on - strand, within MPMX20_00714 at 736.641 kb on + strand, within MPMX20_00714 at 736.644 kb on + strand, within MPMX20_00714 at 736.716 kb on - strand, within MPMX20_00714 at 736.730 kb on - strand, within MPMX20_00714 at 736.773 kb on + strand, within MPMX20_00714 at 736.791 kb on + strand, within MPMX20_00714 at 736.800 kb on + strand, within MPMX20_00714
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A remove 733,597 + +0.1 733,624 + -0.6 733,660 + -0.3 733,697 - MPMX20_00711 0.10 -1.2 733,702 - MPMX20_00711 0.11 -1.1 733,731 + MPMX20_00711 0.14 -1.3 733,732 - MPMX20_00711 0.14 -1.4 733,732 - MPMX20_00711 0.14 -0.1 733,745 + MPMX20_00711 0.15 -0.8 733,811 + MPMX20_00711 0.22 -3.7 733,814 - MPMX20_00711 0.23 -0.1 733,814 - MPMX20_00711 0.23 -2.2 733,829 - MPMX20_00711 0.24 -1.5 733,829 - MPMX20_00711 0.24 -0.8 733,881 + MPMX20_00711 0.30 -1.1 733,881 + MPMX20_00711 0.30 -1.1 733,884 - MPMX20_00711 0.30 -1.2 733,886 + MPMX20_00711 0.30 -2.5 733,886 + MPMX20_00711 0.30 -1.5 733,886 + MPMX20_00711 0.30 -3.0 733,889 - MPMX20_00711 0.31 -0.6 733,889 - MPMX20_00711 0.31 +0.1 733,894 - MPMX20_00711 0.31 -0.3 733,997 - MPMX20_00711 0.42 -0.9 734,103 + MPMX20_00711 0.54 -0.5 734,103 + MPMX20_00711 0.54 -0.6 734,103 + MPMX20_00711 0.54 -0.6 734,104 - MPMX20_00711 0.54 -1.0 734,111 - MPMX20_00711 0.55 -1.1 734,111 - MPMX20_00711 0.55 -2.1 734,111 - MPMX20_00711 0.55 -0.9 734,131 - MPMX20_00711 0.57 -1.7 734,139 + MPMX20_00711 0.58 -0.7 734,154 - MPMX20_00711 0.59 -2.0 734,166 + MPMX20_00711 0.60 -1.4 734,197 + MPMX20_00711 0.64 -1.5 734,205 - MPMX20_00711 0.65 -1.2 734,205 - MPMX20_00711 0.65 -0.4 734,214 + MPMX20_00711 0.66 -0.1 734,234 + MPMX20_00711 0.68 +2.3 734,242 - MPMX20_00711 0.69 -1.4 734,302 + MPMX20_00711 0.75 -0.3 734,302 + MPMX20_00711 0.75 -0.2 734,307 + MPMX20_00711 0.76 +1.2 734,307 + MPMX20_00711 0.76 -2.7 734,307 + MPMX20_00711 0.76 +0.9 734,307 + MPMX20_00711 0.76 +0.3 734,315 - MPMX20_00711 0.76 -0.1 734,315 - MPMX20_00711 0.76 -1.4 734,325 + MPMX20_00711 0.78 -1.8 734,368 + MPMX20_00711 0.82 -0.1 734,369 - MPMX20_00711 0.82 -1.9 734,419 + MPMX20_00711 0.88 -0.7 734,452 - -0.3 734,503 + -1.3 734,527 - -1.5 734,572 + +0.0 734,572 + -0.6 734,587 + -2.3 734,595 + -4.3 734,595 + -1.1 734,603 - -2.5 734,603 - +0.1 734,623 + -0.8 734,661 + -3.1 734,672 - MPMX20_00712 0.10 -1.5 734,680 - MPMX20_00712 0.11 -2.5 734,717 + MPMX20_00712 0.14 -2.9 734,808 - MPMX20_00712 0.21 -0.9 734,820 + MPMX20_00712 0.22 -0.0 734,820 + MPMX20_00712 0.22 +0.3 734,820 + MPMX20_00712 0.22 -0.9 734,820 + MPMX20_00712 0.22 -3.1 734,828 - MPMX20_00712 0.23 -3.0 734,830 + MPMX20_00712 0.23 -0.9 734,830 + MPMX20_00712 0.23 -2.7 734,830 + MPMX20_00712 0.23 -0.5 734,843 + MPMX20_00712 0.24 -2.0 734,846 - MPMX20_00712 0.24 -1.5 735,014 + MPMX20_00712 0.37 -1.7 735,155 + MPMX20_00712 0.48 -0.6 735,163 - MPMX20_00712 0.49 -3.3 735,163 - MPMX20_00712 0.49 -1.3 735,202 + MPMX20_00712 0.52 -1.9 735,391 + MPMX20_00712 0.66 -0.7 735,441 + MPMX20_00712 0.70 -1.7 735,493 + MPMX20_00712 0.74 +0.0 735,514 + MPMX20_00712 0.76 -3.1 735,514 + MPMX20_00712 0.76 -0.3 735,541 - MPMX20_00712 0.78 -1.0 735,570 - MPMX20_00712 0.80 -2.4 735,572 + MPMX20_00712 0.80 -2.6 735,582 + MPMX20_00712 0.81 -2.0 735,582 + MPMX20_00712 0.81 -2.5 735,582 + MPMX20_00712 0.81 +0.1 735,582 + MPMX20_00712 0.81 -2.7 735,582 + MPMX20_00712 0.81 -0.1 735,582 + MPMX20_00712 0.81 -1.8 735,582 + MPMX20_00712 0.81 -2.9 735,582 + MPMX20_00712 0.81 -1.8 735,583 - MPMX20_00712 0.81 -2.3 735,583 - MPMX20_00712 0.81 -3.1 735,590 - MPMX20_00712 0.82 -0.7 735,590 - MPMX20_00712 0.82 -2.6 735,590 - MPMX20_00712 0.82 -0.3 735,590 - MPMX20_00712 0.82 -2.2 735,617 - MPMX20_00712 0.84 -0.1 735,617 - MPMX20_00712 0.84 -2.9 735,650 - MPMX20_00712 0.86 -1.9 735,652 + MPMX20_00712 0.87 -1.4 735,734 - -1.3 735,834 + -0.2 735,834 + +3.7 735,848 + +0.2 735,968 - -0.1 736,007 + -0.3 736,103 - MPMX20_00713 0.23 -0.1 736,103 - MPMX20_00713 0.23 -1.0 736,151 + MPMX20_00713 0.39 +1.1 736,220 - MPMX20_00713 0.62 -0.2 736,272 - MPMX20_00713 0.79 -0.3 736,381 + -0.5 736,444 - -0.2 736,446 - MPMX20_00714 0.10 -0.6 736,474 + MPMX20_00714 0.14 -0.7 736,474 + MPMX20_00714 0.14 -0.4 736,482 - MPMX20_00714 0.15 +2.3 736,532 + MPMX20_00714 0.21 -2.0 736,532 + MPMX20_00714 0.21 +0.2 736,532 + MPMX20_00714 0.21 -0.6 736,532 + MPMX20_00714 0.21 +0.4 736,540 - MPMX20_00714 0.22 +0.3 736,540 - MPMX20_00714 0.22 +0.1 736,540 - MPMX20_00714 0.22 +0.5 736,641 + MPMX20_00714 0.35 -0.2 736,644 + MPMX20_00714 0.36 +0.3 736,716 - MPMX20_00714 0.45 +1.3 736,730 - MPMX20_00714 0.47 -0.4 736,773 + MPMX20_00714 0.52 +0.5 736,791 + MPMX20_00714 0.55 -1.9 736,800 + MPMX20_00714 0.56 +0.1
Or see this region's nucleotide sequence