Experiment: mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00228 and MPMX20_00229 are separated by 68 nucleotides MPMX20_00229 and MPMX20_00230 are separated by 181 nucleotides MPMX20_00230 and MPMX20_00231 are separated by 270 nucleotides
MPMX20_00228: MPMX20_00228 - 23S ribosomal RNA, at 238,052 to 240,954
_00228
MPMX20_00229: MPMX20_00229 - 5S ribosomal RNA, at 241,023 to 241,133
_00229
MPMX20_00230: MPMX20_00230 - Homoserine O-succinyltransferase, at 241,315 to 242,244
_00230
MPMX20_00231: MPMX20_00231 - Malate synthase A, at 242,515 to 244,116
_00231
Position (kb) 241
242
243 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 241.216 kb on + strand at 241.312 kb on - strand at 241.481 kb on - strand, within MPMX20_00230 at 241.481 kb on - strand, within MPMX20_00230 at 241.506 kb on + strand, within MPMX20_00230 at 241.556 kb on + strand, within MPMX20_00230 at 241.639 kb on + strand, within MPMX20_00230 at 241.647 kb on - strand, within MPMX20_00230 at 241.693 kb on + strand, within MPMX20_00230 at 241.711 kb on - strand, within MPMX20_00230 at 241.755 kb on - strand, within MPMX20_00230 at 241.764 kb on - strand, within MPMX20_00230 at 241.811 kb on - strand, within MPMX20_00230 at 241.829 kb on + strand, within MPMX20_00230 at 241.854 kb on - strand, within MPMX20_00230 at 241.922 kb on - strand, within MPMX20_00230 at 241.931 kb on - strand, within MPMX20_00230 at 242.008 kb on + strand, within MPMX20_00230 at 242.064 kb on + strand, within MPMX20_00230 at 242.065 kb on + strand, within MPMX20_00230 at 242.108 kb on - strand, within MPMX20_00230 at 242.471 kb on + strand at 242.549 kb on + strand at 242.801 kb on + strand, within MPMX20_00231 at 242.876 kb on + strand, within MPMX20_00231 at 242.906 kb on - strand, within MPMX20_00231 at 242.980 kb on + strand, within MPMX20_00231 at 242.998 kb on + strand, within MPMX20_00231 at 242.998 kb on + strand, within MPMX20_00231 at 243.006 kb on - strand, within MPMX20_00231 at 243.006 kb on - strand, within MPMX20_00231 at 243.011 kb on - strand, within MPMX20_00231 at 243.027 kb on - strand, within MPMX20_00231 at 243.084 kb on - strand, within MPMX20_00231 at 243.161 kb on - strand, within MPMX20_00231 at 243.165 kb on + strand, within MPMX20_00231
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A remove 241,216 + -0.1 241,312 - -1.5 241,481 - MPMX20_00230 0.18 -2.2 241,481 - MPMX20_00230 0.18 -1.7 241,506 + MPMX20_00230 0.21 -0.7 241,556 + MPMX20_00230 0.26 -1.8 241,639 + MPMX20_00230 0.35 -0.4 241,647 - MPMX20_00230 0.36 -0.6 241,693 + MPMX20_00230 0.41 -1.4 241,711 - MPMX20_00230 0.43 +3.5 241,755 - MPMX20_00230 0.47 -0.9 241,764 - MPMX20_00230 0.48 -4.0 241,811 - MPMX20_00230 0.53 -1.3 241,829 + MPMX20_00230 0.55 -2.2 241,854 - MPMX20_00230 0.58 +0.5 241,922 - MPMX20_00230 0.65 -2.1 241,931 - MPMX20_00230 0.66 -2.2 242,008 + MPMX20_00230 0.75 -2.5 242,064 + MPMX20_00230 0.81 -1.3 242,065 + MPMX20_00230 0.81 -0.4 242,108 - MPMX20_00230 0.85 +1.1 242,471 + +0.5 242,549 + -0.2 242,801 + MPMX20_00231 0.18 +0.5 242,876 + MPMX20_00231 0.23 -0.7 242,906 - MPMX20_00231 0.24 +1.0 242,980 + MPMX20_00231 0.29 -0.1 242,998 + MPMX20_00231 0.30 -0.1 242,998 + MPMX20_00231 0.30 -0.7 243,006 - MPMX20_00231 0.31 +1.7 243,006 - MPMX20_00231 0.31 +0.1 243,011 - MPMX20_00231 0.31 +0.6 243,027 - MPMX20_00231 0.32 +0.5 243,084 - MPMX20_00231 0.36 +0.3 243,161 - MPMX20_00231 0.40 -0.0 243,165 + MPMX20_00231 0.41 -0.7
Or see this region's nucleotide sequence