Experiment: mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00100 and MPMX20_00101 are separated by 2 nucleotides MPMX20_00101 and MPMX20_00102 are separated by 3 nucleotides MPMX20_00102 and MPMX20_00103 overlap by 1 nucleotides
MPMX20_00100: MPMX20_00100 - Formamidopyrimidine-DNA glycosylase, at 101,059 to 101,868
_00100
MPMX20_00101: MPMX20_00101 - Phosphopantetheine adenylyltransferase, at 101,871 to 102,350
_00101
MPMX20_00102: MPMX20_00102 - hypothetical protein, at 102,354 to 103,124
_00102
MPMX20_00103: MPMX20_00103 - 3-deoxy-D-manno-octulosonic acid transferase, at 103,124 to 104,398
_00103
Position (kb)
102
103
104 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 101.367 kb on + strand, within MPMX20_00100 at 101.367 kb on + strand, within MPMX20_00100 at 101.367 kb on + strand, within MPMX20_00100 at 101.367 kb on + strand, within MPMX20_00100 at 101.367 kb on + strand, within MPMX20_00100 at 101.370 kb on - strand, within MPMX20_00100 at 101.372 kb on + strand, within MPMX20_00100 at 101.375 kb on - strand, within MPMX20_00100 at 101.375 kb on - strand, within MPMX20_00100 at 101.415 kb on + strand, within MPMX20_00100 at 101.422 kb on + strand, within MPMX20_00100 at 101.431 kb on + strand, within MPMX20_00100 at 101.442 kb on + strand, within MPMX20_00100 at 101.447 kb on + strand, within MPMX20_00100 at 101.447 kb on + strand, within MPMX20_00100 at 101.452 kb on + strand, within MPMX20_00100 at 101.488 kb on + strand, within MPMX20_00100 at 101.598 kb on + strand, within MPMX20_00100 at 101.630 kb on + strand, within MPMX20_00100 at 101.632 kb on + strand, within MPMX20_00100 at 101.638 kb on - strand, within MPMX20_00100 at 101.638 kb on - strand, within MPMX20_00100 at 101.639 kb on + strand, within MPMX20_00100 at 101.640 kb on - strand, within MPMX20_00100 at 101.642 kb on - strand, within MPMX20_00100 at 101.660 kb on - strand, within MPMX20_00100 at 101.671 kb on + strand, within MPMX20_00100 at 101.679 kb on - strand, within MPMX20_00100 at 101.692 kb on - strand, within MPMX20_00100 at 101.692 kb on - strand, within MPMX20_00100 at 101.705 kb on + strand, within MPMX20_00100 at 101.713 kb on - strand, within MPMX20_00100 at 101.784 kb on + strand, within MPMX20_00100 at 101.844 kb on + strand at 102.393 kb on - strand at 102.420 kb on - strand at 102.577 kb on - strand, within MPMX20_00102 at 102.748 kb on - strand, within MPMX20_00102 at 102.791 kb on + strand, within MPMX20_00102 at 102.791 kb on + strand, within MPMX20_00102 at 102.798 kb on + strand, within MPMX20_00102 at 102.798 kb on + strand, within MPMX20_00102 at 102.806 kb on - strand, within MPMX20_00102 at 102.867 kb on - strand, within MPMX20_00102 at 102.867 kb on - strand, within MPMX20_00102 at 102.880 kb on - strand, within MPMX20_00102 at 102.880 kb on - strand, within MPMX20_00102 at 102.907 kb on + strand, within MPMX20_00102 at 102.915 kb on - strand, within MPMX20_00102 at 102.915 kb on - strand, within MPMX20_00102 at 102.961 kb on + strand, within MPMX20_00102 at 103.107 kb on + strand at 103.138 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in R2A remove 101,367 + MPMX20_00100 0.38 -3.0 101,367 + MPMX20_00100 0.38 +0.2 101,367 + MPMX20_00100 0.38 -0.0 101,367 + MPMX20_00100 0.38 -0.1 101,367 + MPMX20_00100 0.38 +0.3 101,370 - MPMX20_00100 0.38 +0.0 101,372 + MPMX20_00100 0.39 -0.6 101,375 - MPMX20_00100 0.39 +2.4 101,375 - MPMX20_00100 0.39 +0.3 101,415 + MPMX20_00100 0.44 -0.1 101,422 + MPMX20_00100 0.45 -0.3 101,431 + MPMX20_00100 0.46 -1.4 101,442 + MPMX20_00100 0.47 -1.1 101,447 + MPMX20_00100 0.48 +0.7 101,447 + MPMX20_00100 0.48 -0.3 101,452 + MPMX20_00100 0.49 +0.5 101,488 + MPMX20_00100 0.53 -0.4 101,598 + MPMX20_00100 0.67 +0.0 101,630 + MPMX20_00100 0.70 +0.7 101,632 + MPMX20_00100 0.71 +0.6 101,638 - MPMX20_00100 0.71 +0.2 101,638 - MPMX20_00100 0.71 +1.9 101,639 + MPMX20_00100 0.72 -0.2 101,640 - MPMX20_00100 0.72 -1.0 101,642 - MPMX20_00100 0.72 +0.2 101,660 - MPMX20_00100 0.74 +0.1 101,671 + MPMX20_00100 0.76 +0.1 101,679 - MPMX20_00100 0.77 -0.1 101,692 - MPMX20_00100 0.78 -0.4 101,692 - MPMX20_00100 0.78 +1.9 101,705 + MPMX20_00100 0.80 -1.1 101,713 - MPMX20_00100 0.81 -0.1 101,784 + MPMX20_00100 0.90 +0.1 101,844 + +0.5 102,393 - -1.7 102,420 - -2.2 102,577 - MPMX20_00102 0.29 -1.3 102,748 - MPMX20_00102 0.51 +2.3 102,791 + MPMX20_00102 0.57 -0.5 102,791 + MPMX20_00102 0.57 +1.9 102,798 + MPMX20_00102 0.58 +1.9 102,798 + MPMX20_00102 0.58 +0.3 102,806 - MPMX20_00102 0.59 +0.3 102,867 - MPMX20_00102 0.67 -0.1 102,867 - MPMX20_00102 0.67 +0.9 102,880 - MPMX20_00102 0.68 -0.7 102,880 - MPMX20_00102 0.68 +0.3 102,907 + MPMX20_00102 0.72 +0.9 102,915 - MPMX20_00102 0.73 -2.5 102,915 - MPMX20_00102 0.73 -1.8 102,961 + MPMX20_00102 0.79 +1.9 103,107 + +0.9 103,138 - +0.1
Or see this region's nucleotide sequence