Experiment: mutant library grown in Supernatant; Achromobacter sp. TBS_048 grown in R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00875 and MPMX20_00876 overlap by 4 nucleotides MPMX20_00876 and MPMX20_00877 are separated by 33 nucleotides MPMX20_00877 and MPMX20_00878 are separated by 53 nucleotides
MPMX20_00875: MPMX20_00875 - putative protein YaeQ, at 925,856 to 926,401
_00875
MPMX20_00876: MPMX20_00876 - Peptidyl-tRNA hydrolase ArfB, at 926,398 to 926,820
_00876
MPMX20_00877: MPMX20_00877 - Lipoprotein NlpE, at 926,854 to 927,552
_00877
MPMX20_00878: MPMX20_00878 - Proline--tRNA ligase, at 927,606 to 929,324
_00878
Position (kb) 926
927
928 Strain fitness (log2 ratio) -1
0
1
2 at 925.926 kb on - strand, within MPMX20_00875 at 925.926 kb on - strand, within MPMX20_00875 at 926.073 kb on + strand, within MPMX20_00875 at 926.151 kb on + strand, within MPMX20_00875 at 926.223 kb on - strand, within MPMX20_00875 at 926.271 kb on + strand, within MPMX20_00875 at 926.319 kb on + strand, within MPMX20_00875 at 926.331 kb on + strand, within MPMX20_00875 at 926.416 kb on + strand at 926.476 kb on + strand, within MPMX20_00876 at 926.570 kb on + strand, within MPMX20_00876 at 926.584 kb on - strand, within MPMX20_00876 at 926.617 kb on + strand, within MPMX20_00876 at 926.622 kb on + strand, within MPMX20_00876 at 926.730 kb on - strand, within MPMX20_00876 at 926.735 kb on + strand, within MPMX20_00876 at 926.745 kb on + strand, within MPMX20_00876 at 926.871 kb on - strand at 926.990 kb on + strand, within MPMX20_00877 at 927.000 kb on - strand, within MPMX20_00877 at 927.000 kb on - strand, within MPMX20_00877 at 927.076 kb on - strand, within MPMX20_00877 at 927.123 kb on - strand, within MPMX20_00877 at 927.195 kb on - strand, within MPMX20_00877 at 927.242 kb on + strand, within MPMX20_00877 at 927.250 kb on - strand, within MPMX20_00877 at 927.381 kb on + strand, within MPMX20_00877 at 927.381 kb on + strand, within MPMX20_00877 at 927.422 kb on + strand, within MPMX20_00877 at 927.422 kb on + strand, within MPMX20_00877 at 927.425 kb on - strand, within MPMX20_00877 at 927.476 kb on + strand, within MPMX20_00877 at 927.476 kb on + strand, within MPMX20_00877 at 927.484 kb on - strand at 927.529 kb on + strand at 927.537 kb on - strand at 927.543 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Achromobacter sp. TBS_048 grown in R2A remove 925,926 - MPMX20_00875 0.13 +0.7 925,926 - MPMX20_00875 0.13 -0.5 926,073 + MPMX20_00875 0.40 +0.0 926,151 + MPMX20_00875 0.54 +0.5 926,223 - MPMX20_00875 0.67 +0.1 926,271 + MPMX20_00875 0.76 -0.9 926,319 + MPMX20_00875 0.85 +0.2 926,331 + MPMX20_00875 0.87 -0.3 926,416 + +0.1 926,476 + MPMX20_00876 0.18 -0.1 926,570 + MPMX20_00876 0.41 -1.0 926,584 - MPMX20_00876 0.44 -0.5 926,617 + MPMX20_00876 0.52 -0.0 926,622 + MPMX20_00876 0.53 +0.6 926,730 - MPMX20_00876 0.78 +0.2 926,735 + MPMX20_00876 0.80 +1.7 926,745 + MPMX20_00876 0.82 +0.8 926,871 - +0.0 926,990 + MPMX20_00877 0.19 +0.2 927,000 - MPMX20_00877 0.21 +1.8 927,000 - MPMX20_00877 0.21 +1.5 927,076 - MPMX20_00877 0.32 +0.6 927,123 - MPMX20_00877 0.38 -0.3 927,195 - MPMX20_00877 0.49 -0.1 927,242 + MPMX20_00877 0.56 +0.4 927,250 - MPMX20_00877 0.57 +0.2 927,381 + MPMX20_00877 0.75 +0.3 927,381 + MPMX20_00877 0.75 -0.3 927,422 + MPMX20_00877 0.81 +0.1 927,422 + MPMX20_00877 0.81 -0.1 927,425 - MPMX20_00877 0.82 +1.7 927,476 + MPMX20_00877 0.89 +0.5 927,476 + MPMX20_00877 0.89 +0.0 927,484 - -0.7 927,529 + +0.5 927,537 - -0.3 927,543 - -0.8
Or see this region's nucleotide sequence