Experiment: mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_03659 and MPMX20_03660 are separated by 371 nucleotides MPMX20_03660 and MPMX20_03661 are separated by 15 nucleotides MPMX20_03661 and MPMX20_03662 overlap by 4 nucleotides MPMX20_03662 and MPMX20_03663 are separated by 630 nucleotides
MPMX20_03659: MPMX20_03659 - putative glucarate transporter, at 3,853,671 to 3,855,026
_03659
MPMX20_03660: MPMX20_03660 - hypothetical protein, at 3,855,398 to 3,855,847
_03660
MPMX20_03661: MPMX20_03661 - tRNA pseudouridine synthase C, at 3,855,863 to 3,856,648
_03661
MPMX20_03662: MPMX20_03662 - putative protein YqcC, at 3,856,645 to 3,856,974
_03662
MPMX20_03663: MPMX20_03663 - Protein Syd, at 3,857,605 to 3,858,150
_03663
Position (kb)
3855
3856
3857 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3854.867 kb on + strand, within MPMX20_03659 at 3854.875 kb on - strand, within MPMX20_03659 at 3854.912 kb on + strand at 3854.944 kb on + strand at 3854.965 kb on + strand at 3854.968 kb on - strand at 3854.968 kb on - strand at 3854.968 kb on - strand at 3854.968 kb on - strand at 3854.973 kb on - strand at 3854.990 kb on + strand at 3854.990 kb on + strand at 3854.990 kb on + strand at 3854.990 kb on + strand at 3854.990 kb on + strand at 3854.990 kb on + strand at 3854.998 kb on - strand at 3854.998 kb on - strand at 3854.998 kb on - strand at 3854.998 kb on - strand at 3855.011 kb on + strand at 3855.026 kb on + strand at 3855.044 kb on - strand at 3855.049 kb on - strand at 3855.061 kb on + strand at 3855.064 kb on - strand at 3855.066 kb on + strand at 3855.074 kb on - strand at 3855.122 kb on + strand at 3855.132 kb on + strand at 3855.140 kb on - strand at 3855.181 kb on - strand at 3855.189 kb on + strand at 3855.194 kb on + strand at 3855.194 kb on + strand at 3855.202 kb on - strand at 3855.202 kb on - strand at 3855.225 kb on - strand at 3855.254 kb on + strand at 3855.262 kb on - strand at 3855.262 kb on - strand at 3855.262 kb on - strand at 3855.262 kb on - strand at 3855.272 kb on - strand at 3855.409 kb on + strand at 3855.409 kb on + strand at 3855.409 kb on + strand at 3855.419 kb on + strand at 3855.419 kb on + strand at 3855.419 kb on + strand at 3855.427 kb on - strand at 3855.429 kb on - strand at 3855.452 kb on - strand, within MPMX20_03660 at 3855.466 kb on - strand, within MPMX20_03660 at 3855.564 kb on + strand, within MPMX20_03660 at 3855.598 kb on - strand, within MPMX20_03660 at 3855.703 kb on - strand, within MPMX20_03660 at 3855.758 kb on + strand, within MPMX20_03660 at 3855.758 kb on + strand, within MPMX20_03660 at 3855.766 kb on - strand, within MPMX20_03660 at 3855.786 kb on - strand, within MPMX20_03660 at 3855.809 kb on + strand at 3855.809 kb on + strand at 3855.844 kb on - strand at 3855.846 kb on - strand at 3855.882 kb on + strand at 3855.974 kb on + strand, within MPMX20_03661 at 3855.982 kb on - strand, within MPMX20_03661 at 3856.162 kb on - strand, within MPMX20_03661 at 3856.409 kb on - strand, within MPMX20_03661 at 3856.411 kb on + strand, within MPMX20_03661 at 3856.419 kb on - strand, within MPMX20_03661 at 3856.462 kb on + strand, within MPMX20_03661 at 3856.468 kb on + strand, within MPMX20_03661 at 3856.533 kb on + strand, within MPMX20_03661 at 3856.616 kb on + strand at 3856.712 kb on + strand, within MPMX20_03662 at 3856.722 kb on + strand, within MPMX20_03662 at 3856.781 kb on + strand, within MPMX20_03662 at 3856.783 kb on + strand, within MPMX20_03662 at 3856.806 kb on + strand, within MPMX20_03662 at 3856.844 kb on - strand, within MPMX20_03662 at 3856.849 kb on - strand, within MPMX20_03662 at 3856.945 kb on - strand at 3856.981 kb on - strand at 3857.006 kb on + strand at 3857.008 kb on + strand at 3857.008 kb on + strand at 3857.008 kb on + strand at 3857.014 kb on - strand at 3857.016 kb on - strand at 3857.139 kb on + strand at 3857.157 kb on - strand at 3857.178 kb on + strand at 3857.233 kb on + strand at 3857.270 kb on + strand at 3857.334 kb on - strand at 3857.415 kb on - strand at 3857.473 kb on - strand at 3857.473 kb on - strand at 3857.564 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in LB remove 3,854,867 + MPMX20_03659 0.88 +0.2 3,854,875 - MPMX20_03659 0.89 +0.0 3,854,912 + +2.9 3,854,944 + -0.2 3,854,965 + +0.2 3,854,968 - -0.2 3,854,968 - -0.3 3,854,968 - -0.3 3,854,968 - -0.2 3,854,973 - -0.2 3,854,990 + -0.0 3,854,990 + +0.6 3,854,990 + +0.1 3,854,990 + -0.0 3,854,990 + -0.2 3,854,990 + +0.4 3,854,998 - -0.3 3,854,998 - -0.5 3,854,998 - -0.6 3,854,998 - -0.1 3,855,011 + -0.7 3,855,026 + +0.8 3,855,044 - +0.5 3,855,049 - +1.3 3,855,061 + +0.2 3,855,064 - -0.7 3,855,066 + -0.1 3,855,074 - -0.6 3,855,122 + +0.8 3,855,132 + +0.1 3,855,140 - +0.4 3,855,181 - +0.7 3,855,189 + -0.0 3,855,194 + -1.0 3,855,194 + -0.3 3,855,202 - -0.2 3,855,202 - +0.8 3,855,225 - +0.2 3,855,254 + -0.6 3,855,262 - -0.6 3,855,262 - +0.8 3,855,262 - -0.0 3,855,262 - -0.9 3,855,272 - +0.4 3,855,409 + -0.2 3,855,409 + +0.6 3,855,409 + -0.9 3,855,419 + +0.2 3,855,419 + -0.1 3,855,419 + +0.4 3,855,427 - +0.4 3,855,429 - +0.6 3,855,452 - MPMX20_03660 0.12 -1.4 3,855,466 - MPMX20_03660 0.15 -0.4 3,855,564 + MPMX20_03660 0.37 +1.9 3,855,598 - MPMX20_03660 0.44 -1.9 3,855,703 - MPMX20_03660 0.68 +0.0 3,855,758 + MPMX20_03660 0.80 -0.5 3,855,758 + MPMX20_03660 0.80 -0.8 3,855,766 - MPMX20_03660 0.82 +0.3 3,855,786 - MPMX20_03660 0.86 -0.6 3,855,809 + -0.6 3,855,809 + +0.6 3,855,844 - +0.2 3,855,846 - +0.6 3,855,882 + -0.6 3,855,974 + MPMX20_03661 0.14 +0.2 3,855,982 - MPMX20_03661 0.15 +0.8 3,856,162 - MPMX20_03661 0.38 +0.3 3,856,409 - MPMX20_03661 0.69 +0.4 3,856,411 + MPMX20_03661 0.70 -0.7 3,856,419 - MPMX20_03661 0.71 +1.1 3,856,462 + MPMX20_03661 0.76 -0.2 3,856,468 + MPMX20_03661 0.77 -0.1 3,856,533 + MPMX20_03661 0.85 -0.1 3,856,616 + +1.2 3,856,712 + MPMX20_03662 0.20 -0.7 3,856,722 + MPMX20_03662 0.23 +1.4 3,856,781 + MPMX20_03662 0.41 +0.5 3,856,783 + MPMX20_03662 0.42 +0.2 3,856,806 + MPMX20_03662 0.49 -0.8 3,856,844 - MPMX20_03662 0.60 +0.1 3,856,849 - MPMX20_03662 0.62 +0.3 3,856,945 - +0.3 3,856,981 - -1.0 3,857,006 + +0.1 3,857,008 + +0.9 3,857,008 + -0.3 3,857,008 + -0.6 3,857,014 - +0.1 3,857,016 - -0.5 3,857,139 + +0.1 3,857,157 - -0.1 3,857,178 + +0.9 3,857,233 + -1.2 3,857,270 + +0.3 3,857,334 - -2.5 3,857,415 - +0.5 3,857,473 - +0.4 3,857,473 - -0.2 3,857,564 + -0.0
Or see this region's nucleotide sequence