Experiment: mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01105 and MPMX20_01106 are separated by 219 nucleotides MPMX20_01106 and MPMX20_01107 are separated by 1 nucleotides
MPMX20_01105: MPMX20_01105 - PTS system glucose-specific EIICBA component, at 1,164,397 to 1,165,896
_01105
MPMX20_01106: MPMX20_01106 - putative protein YbcJ, at 1,166,116 to 1,166,328
_01106
MPMX20_01107: MPMX20_01107 - Bifunctional protein FolD protein, at 1,166,330 to 1,167,196
_01107
Position (kb)
1166
1167 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1165.182 kb on - strand, within MPMX20_01105 at 1165.219 kb on - strand, within MPMX20_01105 at 1165.219 kb on - strand, within MPMX20_01105 at 1165.244 kb on + strand, within MPMX20_01105 at 1165.262 kb on - strand, within MPMX20_01105 at 1165.275 kb on + strand, within MPMX20_01105 at 1165.280 kb on + strand, within MPMX20_01105 at 1165.280 kb on + strand, within MPMX20_01105 at 1165.288 kb on - strand, within MPMX20_01105 at 1165.303 kb on + strand, within MPMX20_01105 at 1165.390 kb on - strand, within MPMX20_01105 at 1165.478 kb on - strand, within MPMX20_01105 at 1165.609 kb on + strand, within MPMX20_01105 at 1165.617 kb on - strand, within MPMX20_01105 at 1165.700 kb on - strand, within MPMX20_01105 at 1165.701 kb on - strand, within MPMX20_01105 at 1165.737 kb on + strand, within MPMX20_01105 at 1165.777 kb on - strand at 1165.887 kb on - strand at 1165.893 kb on - strand at 1165.893 kb on - strand at 1166.070 kb on - strand at 1166.076 kb on - strand at 1166.121 kb on - strand at 1166.188 kb on + strand, within MPMX20_01106 at 1166.196 kb on - strand, within MPMX20_01106 at 1166.201 kb on + strand, within MPMX20_01106 at 1166.201 kb on - strand, within MPMX20_01106 at 1166.209 kb on - strand, within MPMX20_01106 at 1166.234 kb on + strand, within MPMX20_01106 at 1166.297 kb on - strand, within MPMX20_01106 at 1166.328 kb on + strand at 1166.346 kb on - strand at 1166.348 kb on + strand at 1166.354 kb on - strand at 1166.364 kb on - strand at 1167.210 kb on - strand at 1167.268 kb on + strand at 1167.268 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.270 kb on + strand at 1167.277 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.278 kb on - strand at 1167.299 kb on - strand at 1167.303 kb on + strand at 1167.311 kb on - strand at 1167.314 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in LB remove 1,165,182 - MPMX20_01105 0.52 +0.4 1,165,219 - MPMX20_01105 0.55 +0.2 1,165,219 - MPMX20_01105 0.55 +0.9 1,165,244 + MPMX20_01105 0.56 -0.8 1,165,262 - MPMX20_01105 0.58 -0.6 1,165,275 + MPMX20_01105 0.59 -0.5 1,165,280 + MPMX20_01105 0.59 -0.1 1,165,280 + MPMX20_01105 0.59 +1.1 1,165,288 - MPMX20_01105 0.59 +0.3 1,165,303 + MPMX20_01105 0.60 +0.4 1,165,390 - MPMX20_01105 0.66 -0.2 1,165,478 - MPMX20_01105 0.72 -0.3 1,165,609 + MPMX20_01105 0.81 +0.1 1,165,617 - MPMX20_01105 0.81 +0.1 1,165,700 - MPMX20_01105 0.87 -0.3 1,165,701 - MPMX20_01105 0.87 -0.1 1,165,737 + MPMX20_01105 0.89 +0.4 1,165,777 - +0.2 1,165,887 - -0.1 1,165,893 - -2.9 1,165,893 - +0.2 1,166,070 - +0.7 1,166,076 - -0.6 1,166,121 - +0.7 1,166,188 + MPMX20_01106 0.34 -0.2 1,166,196 - MPMX20_01106 0.38 +1.1 1,166,201 + MPMX20_01106 0.40 -2.2 1,166,201 - MPMX20_01106 0.40 +1.9 1,166,209 - MPMX20_01106 0.44 -0.1 1,166,234 + MPMX20_01106 0.55 +0.2 1,166,297 - MPMX20_01106 0.85 +0.7 1,166,328 + -0.1 1,166,346 - -0.8 1,166,348 + -2.0 1,166,354 - +0.2 1,166,364 - -0.3 1,167,210 - -0.9 1,167,268 + +0.1 1,167,268 + -0.5 1,167,270 + -0.3 1,167,270 + -0.6 1,167,270 + +0.4 1,167,270 + +0.2 1,167,270 + -1.0 1,167,270 + -0.6 1,167,270 + +0.5 1,167,270 + +1.4 1,167,270 + -0.8 1,167,270 + -0.5 1,167,270 + +0.1 1,167,270 + -0.1 1,167,270 + +0.1 1,167,270 + +0.7 1,167,270 + -1.1 1,167,270 + -0.1 1,167,270 + -0.3 1,167,270 + +1.9 1,167,270 + +0.0 1,167,270 + -1.2 1,167,270 + +0.1 1,167,270 + -1.8 1,167,270 + +0.8 1,167,270 + +0.5 1,167,270 + +0.8 1,167,270 + +0.0 1,167,270 + +0.3 1,167,270 + +0.2 1,167,270 + -0.5 1,167,270 + -0.7 1,167,270 + +0.1 1,167,270 + -0.9 1,167,270 + -0.4 1,167,270 + +0.3 1,167,270 + -1.1 1,167,277 - +1.0 1,167,278 - -0.1 1,167,278 - +1.4 1,167,278 - +0.3 1,167,278 - +0.5 1,167,278 - -0.8 1,167,278 - -0.9 1,167,278 - +0.4 1,167,278 - -0.0 1,167,278 - +0.4 1,167,278 - +1.9 1,167,278 - +1.0 1,167,278 - +0.3 1,167,278 - -0.4 1,167,278 - -0.4 1,167,278 - -0.1 1,167,278 - -0.4 1,167,278 - +0.2 1,167,278 - -0.2 1,167,278 - +0.5 1,167,299 - -0.2 1,167,303 + -0.4 1,167,311 - +1.2 1,167,314 + +0.0
Or see this region's nucleotide sequence