Experiment: mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00491 and MPMX20_00492 are separated by 190 nucleotides MPMX20_00492 and MPMX20_00493 overlap by 7 nucleotides MPMX20_00493 and MPMX20_00494 are separated by 324 nucleotides MPMX20_00494 and MPMX20_00495 are separated by 78 nucleotides
MPMX20_00491: MPMX20_00491 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase, at 503,741 to 505,684
_00491
MPMX20_00492: MPMX20_00492 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 505,875 to 506,615
_00492
MPMX20_00493: MPMX20_00493 - putative protein YtfJ, at 506,609 to 507,166
_00493
MPMX20_00494: MPMX20_00494 - hypothetical protein, at 507,491 to 507,697
_00494
MPMX20_00495: MPMX20_00495 - hypothetical protein, at 507,776 to 509,113
_00495
Position (kb) 506
507
508 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 505.615 kb on + strand at 505.690 kb on - strand at 505.692 kb on - strand at 505.937 kb on - strand at 505.947 kb on - strand at 505.976 kb on - strand, within MPMX20_00492 at 506.033 kb on - strand, within MPMX20_00492 at 506.078 kb on + strand, within MPMX20_00492 at 506.098 kb on - strand, within MPMX20_00492 at 506.134 kb on + strand, within MPMX20_00492 at 506.160 kb on + strand, within MPMX20_00492 at 506.231 kb on + strand, within MPMX20_00492 at 506.238 kb on + strand, within MPMX20_00492 at 506.238 kb on + strand, within MPMX20_00492 at 506.246 kb on - strand, within MPMX20_00492 at 506.246 kb on - strand, within MPMX20_00492 at 506.248 kb on + strand, within MPMX20_00492 at 506.258 kb on + strand, within MPMX20_00492 at 506.271 kb on + strand, within MPMX20_00492 at 506.297 kb on + strand, within MPMX20_00492 at 506.336 kb on - strand, within MPMX20_00492 at 506.361 kb on + strand, within MPMX20_00492 at 506.389 kb on - strand, within MPMX20_00492 at 506.399 kb on - strand, within MPMX20_00492 at 506.426 kb on - strand, within MPMX20_00492 at 506.443 kb on + strand, within MPMX20_00492 at 506.451 kb on - strand, within MPMX20_00492 at 506.453 kb on + strand, within MPMX20_00492 at 506.461 kb on - strand, within MPMX20_00492 at 506.461 kb on - strand, within MPMX20_00492 at 506.518 kb on - strand, within MPMX20_00492 at 506.532 kb on + strand, within MPMX20_00492 at 506.558 kb on + strand at 506.558 kb on + strand at 506.558 kb on + strand at 506.566 kb on - strand at 506.566 kb on - strand at 506.568 kb on - strand at 506.656 kb on - strand at 506.663 kb on + strand at 506.663 kb on + strand at 506.687 kb on + strand, within MPMX20_00493 at 506.689 kb on + strand, within MPMX20_00493 at 506.697 kb on - strand, within MPMX20_00493 at 506.743 kb on + strand, within MPMX20_00493 at 506.763 kb on - strand, within MPMX20_00493 at 506.819 kb on - strand, within MPMX20_00493 at 506.915 kb on + strand, within MPMX20_00493 at 506.969 kb on + strand, within MPMX20_00493 at 506.979 kb on + strand, within MPMX20_00493 at 506.987 kb on - strand, within MPMX20_00493 at 507.003 kb on + strand, within MPMX20_00493 at 507.006 kb on - strand, within MPMX20_00493 at 507.008 kb on + strand, within MPMX20_00493 at 507.011 kb on - strand, within MPMX20_00493 at 507.016 kb on - strand, within MPMX20_00493 at 507.116 kb on - strand at 507.130 kb on - strand at 507.148 kb on + strand at 507.148 kb on + strand at 507.169 kb on - strand at 507.181 kb on + strand at 507.181 kb on + strand at 507.235 kb on + strand at 507.300 kb on + strand at 507.300 kb on + strand at 507.300 kb on + strand at 507.300 kb on + strand at 507.301 kb on + strand at 507.305 kb on + strand at 507.308 kb on - strand at 507.308 kb on - strand at 507.310 kb on + strand at 507.313 kb on - strand at 507.315 kb on + strand at 507.315 kb on + strand at 507.323 kb on - strand at 507.323 kb on - strand at 507.323 kb on - strand at 507.379 kb on + strand at 507.381 kb on + strand at 507.381 kb on + strand at 507.389 kb on + strand at 507.389 kb on + strand at 507.389 kb on + strand at 507.389 kb on - strand at 507.389 kb on - strand at 507.389 kb on - strand at 507.394 kb on + strand at 507.397 kb on - strand at 507.397 kb on - strand at 507.397 kb on - strand at 507.397 kb on - strand at 507.397 kb on - strand at 507.397 kb on - strand at 507.397 kb on - strand at 507.397 kb on - strand at 507.397 kb on - strand at 507.397 kb on - strand at 507.399 kb on + strand at 507.399 kb on + strand at 507.399 kb on + strand at 507.402 kb on - strand at 507.407 kb on - strand at 507.440 kb on - strand at 507.455 kb on - strand at 507.465 kb on - strand at 507.516 kb on - strand, within MPMX20_00494 at 507.567 kb on - strand, within MPMX20_00494 at 507.649 kb on + strand, within MPMX20_00494 at 507.669 kb on - strand, within MPMX20_00494 at 507.691 kb on + strand at 507.691 kb on + strand at 507.691 kb on + strand at 507.699 kb on - strand at 507.699 kb on - strand at 507.699 kb on - strand at 507.705 kb on + strand at 507.708 kb on + strand at 507.749 kb on + strand at 507.765 kb on - strand at 507.864 kb on + strand at 508.053 kb on + strand, within MPMX20_00495 at 508.053 kb on + strand, within MPMX20_00495 at 508.054 kb on + strand, within MPMX20_00495 at 508.061 kb on - strand, within MPMX20_00495 at 508.062 kb on - strand, within MPMX20_00495 at 508.078 kb on - strand, within MPMX20_00495 at 508.112 kb on + strand, within MPMX20_00495 at 508.118 kb on - strand, within MPMX20_00495 at 508.141 kb on + strand, within MPMX20_00495 at 508.141 kb on + strand, within MPMX20_00495 at 508.149 kb on - strand, within MPMX20_00495 at 508.149 kb on - strand, within MPMX20_00495 at 508.160 kb on - strand, within MPMX20_00495
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Variovorax sp. TBS_050B grown in LB remove 505,615 + -0.8 505,690 - -0.5 505,692 - +0.2 505,937 - +0.0 505,947 - +2.9 505,976 - MPMX20_00492 0.14 +0.1 506,033 - MPMX20_00492 0.21 +0.0 506,078 + MPMX20_00492 0.27 -1.7 506,098 - MPMX20_00492 0.30 +0.3 506,134 + MPMX20_00492 0.35 +0.3 506,160 + MPMX20_00492 0.38 -1.9 506,231 + MPMX20_00492 0.48 -0.0 506,238 + MPMX20_00492 0.49 -0.1 506,238 + MPMX20_00492 0.49 -0.4 506,246 - MPMX20_00492 0.50 -0.2 506,246 - MPMX20_00492 0.50 -1.3 506,248 + MPMX20_00492 0.50 +0.2 506,258 + MPMX20_00492 0.52 +0.7 506,271 + MPMX20_00492 0.53 +0.4 506,297 + MPMX20_00492 0.57 +0.0 506,336 - MPMX20_00492 0.62 +0.8 506,361 + MPMX20_00492 0.66 -1.9 506,389 - MPMX20_00492 0.69 +0.9 506,399 - MPMX20_00492 0.71 -0.1 506,426 - MPMX20_00492 0.74 +0.1 506,443 + MPMX20_00492 0.77 +0.9 506,451 - MPMX20_00492 0.78 -0.2 506,453 + MPMX20_00492 0.78 -0.7 506,461 - MPMX20_00492 0.79 +0.3 506,461 - MPMX20_00492 0.79 -0.5 506,518 - MPMX20_00492 0.87 -1.3 506,532 + MPMX20_00492 0.89 -0.2 506,558 + +0.5 506,558 + -0.6 506,558 + +0.8 506,566 - +0.4 506,566 - +0.4 506,568 - -0.5 506,656 - -0.1 506,663 + +0.0 506,663 + +0.1 506,687 + MPMX20_00493 0.14 -2.0 506,689 + MPMX20_00493 0.14 -0.4 506,697 - MPMX20_00493 0.16 +0.3 506,743 + MPMX20_00493 0.24 +0.9 506,763 - MPMX20_00493 0.28 +0.9 506,819 - MPMX20_00493 0.38 +0.4 506,915 + MPMX20_00493 0.55 -0.2 506,969 + MPMX20_00493 0.65 -0.8 506,979 + MPMX20_00493 0.66 +0.1 506,987 - MPMX20_00493 0.68 -0.2 507,003 + MPMX20_00493 0.71 +0.1 507,006 - MPMX20_00493 0.71 +2.9 507,008 + MPMX20_00493 0.72 +0.4 507,011 - MPMX20_00493 0.72 +2.7 507,016 - MPMX20_00493 0.73 +0.4 507,116 - -0.5 507,130 - +0.8 507,148 + +0.2 507,148 + -0.7 507,169 - +3.6 507,181 + +0.3 507,181 + -0.9 507,235 + +0.4 507,300 + -1.0 507,300 + -0.2 507,300 + -0.3 507,300 + +0.5 507,301 + +2.3 507,305 + -0.1 507,308 - +0.0 507,308 - -0.1 507,310 + +0.6 507,313 - -0.3 507,315 + +0.1 507,315 + +1.7 507,323 - +0.5 507,323 - +1.7 507,323 - -0.0 507,379 + -0.7 507,381 + +0.8 507,381 + +0.2 507,389 + -0.1 507,389 + -0.1 507,389 + -0.2 507,389 - +1.0 507,389 - +1.1 507,389 - -1.1 507,394 + +0.3 507,397 - +0.2 507,397 - +0.5 507,397 - +0.7 507,397 - +0.2 507,397 - +1.0 507,397 - +0.6 507,397 - -0.5 507,397 - +0.0 507,397 - +0.1 507,397 - +0.9 507,399 + -1.7 507,399 + -0.1 507,399 + +0.1 507,402 - +0.3 507,407 - -0.0 507,440 - +0.2 507,455 - -1.3 507,465 - -0.2 507,516 - MPMX20_00494 0.12 -0.4 507,567 - MPMX20_00494 0.37 +0.2 507,649 + MPMX20_00494 0.76 -0.5 507,669 - MPMX20_00494 0.86 +0.9 507,691 + -0.5 507,691 + +0.0 507,691 + +0.2 507,699 - +0.4 507,699 - -0.7 507,699 - -0.6 507,705 + +0.5 507,708 + -1.1 507,749 + -0.3 507,765 - -1.0 507,864 + -0.7 508,053 + MPMX20_00495 0.21 +0.1 508,053 + MPMX20_00495 0.21 +0.8 508,054 + MPMX20_00495 0.21 -0.5 508,061 - MPMX20_00495 0.21 +0.2 508,062 - MPMX20_00495 0.21 -1.4 508,078 - MPMX20_00495 0.23 -1.3 508,112 + MPMX20_00495 0.25 -0.9 508,118 - MPMX20_00495 0.26 -0.4 508,141 + MPMX20_00495 0.27 +1.3 508,141 + MPMX20_00495 0.27 +0.5 508,149 - MPMX20_00495 0.28 -1.3 508,149 - MPMX20_00495 0.28 -0.1 508,160 - MPMX20_00495 0.29 +0.1
Or see this region's nucleotide sequence