Experiment: mutant library grown in Supernatant; Inquilinus sp. 005-A5 grown in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00158 and MPMX20_00159 are separated by 180 nucleotides MPMX20_00159 and MPMX20_00160 are separated by 314 nucleotides
MPMX20_00158: MPMX20_00158 - Valine--pyruvate aminotransferase, at 161,076 to 162,332
_00158
MPMX20_00159: MPMX20_00159 - Periplasmic alpha-amylase, at 162,513 to 164,543
_00159
MPMX20_00160: MPMX20_00160 - hypothetical protein, at 164,858 to 165,682
_00160
Position (kb) 162
163
164
165 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 161.594 kb on + strand, within MPMX20_00158 at 161.594 kb on + strand, within MPMX20_00158 at 161.595 kb on + strand, within MPMX20_00158 at 161.668 kb on + strand, within MPMX20_00158 at 161.668 kb on + strand, within MPMX20_00158 at 161.668 kb on + strand, within MPMX20_00158 at 161.668 kb on + strand, within MPMX20_00158 at 161.676 kb on - strand, within MPMX20_00158 at 161.676 kb on - strand, within MPMX20_00158 at 161.676 kb on - strand, within MPMX20_00158 at 161.743 kb on + strand, within MPMX20_00158 at 161.784 kb on + strand, within MPMX20_00158 at 161.792 kb on - strand, within MPMX20_00158 at 161.792 kb on - strand, within MPMX20_00158 at 161.792 kb on - strand, within MPMX20_00158 at 161.816 kb on + strand, within MPMX20_00158 at 161.843 kb on + strand, within MPMX20_00158 at 161.865 kb on - strand, within MPMX20_00158 at 161.866 kb on - strand, within MPMX20_00158 at 161.888 kb on - strand, within MPMX20_00158 at 161.919 kb on + strand, within MPMX20_00158 at 161.970 kb on + strand, within MPMX20_00158 at 162.060 kb on - strand, within MPMX20_00158 at 162.065 kb on - strand, within MPMX20_00158 at 162.074 kb on + strand, within MPMX20_00158 at 162.082 kb on - strand, within MPMX20_00158 at 162.087 kb on - strand, within MPMX20_00158 at 162.103 kb on + strand, within MPMX20_00158 at 162.103 kb on + strand, within MPMX20_00158 at 162.106 kb on - strand, within MPMX20_00158 at 162.239 kb on + strand at 162.259 kb on + strand at 162.267 kb on - strand at 162.282 kb on + strand at 162.282 kb on + strand at 162.286 kb on - strand at 162.327 kb on + strand at 162.337 kb on + strand at 162.371 kb on + strand at 162.371 kb on + strand at 162.379 kb on - strand at 162.385 kb on - strand at 162.385 kb on - strand at 162.385 kb on - strand at 162.385 kb on - strand at 162.392 kb on + strand at 162.487 kb on + strand at 162.496 kb on - strand at 162.526 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.535 kb on + strand at 162.543 kb on - strand at 162.543 kb on - strand at 162.543 kb on - strand at 162.543 kb on - strand at 162.550 kb on + strand at 162.570 kb on + strand at 162.596 kb on + strand at 162.631 kb on + strand at 162.642 kb on + strand at 162.667 kb on + strand at 162.667 kb on + strand at 162.667 kb on + strand at 162.697 kb on - strand at 162.699 kb on + strand at 162.699 kb on + strand at 162.699 kb on + strand at 162.699 kb on + strand at 162.707 kb on - strand at 162.707 kb on - strand at 162.707 kb on - strand at 162.720 kb on + strand, within MPMX20_00159 at 162.728 kb on + strand, within MPMX20_00159 at 162.733 kb on + strand, within MPMX20_00159 at 162.736 kb on - strand, within MPMX20_00159 at 162.736 kb on - strand, within MPMX20_00159 at 162.743 kb on + strand, within MPMX20_00159 at 162.802 kb on - strand, within MPMX20_00159 at 162.807 kb on - strand, within MPMX20_00159 at 162.817 kb on - strand, within MPMX20_00159 at 162.837 kb on + strand, within MPMX20_00159 at 162.865 kb on - strand, within MPMX20_00159 at 162.875 kb on - strand, within MPMX20_00159 at 162.875 kb on - strand, within MPMX20_00159 at 162.917 kb on + strand, within MPMX20_00159 at 162.934 kb on - strand, within MPMX20_00159 at 162.983 kb on + strand, within MPMX20_00159 at 162.983 kb on + strand, within MPMX20_00159 at 163.055 kb on + strand, within MPMX20_00159 at 163.167 kb on - strand, within MPMX20_00159 at 163.191 kb on + strand, within MPMX20_00159 at 163.201 kb on - strand, within MPMX20_00159 at 163.231 kb on - strand, within MPMX20_00159 at 163.270 kb on + strand, within MPMX20_00159 at 163.271 kb on - strand, within MPMX20_00159 at 163.271 kb on - strand, within MPMX20_00159 at 163.278 kb on - strand, within MPMX20_00159 at 163.278 kb on - strand, within MPMX20_00159 at 163.285 kb on + strand, within MPMX20_00159 at 163.357 kb on - strand, within MPMX20_00159 at 163.357 kb on - strand, within MPMX20_00159 at 163.404 kb on + strand, within MPMX20_00159 at 163.450 kb on + strand, within MPMX20_00159 at 163.473 kb on + strand, within MPMX20_00159 at 163.473 kb on + strand, within MPMX20_00159 at 163.473 kb on + strand, within MPMX20_00159 at 163.473 kb on + strand, within MPMX20_00159 at 163.473 kb on + strand, within MPMX20_00159 at 163.481 kb on - strand, within MPMX20_00159 at 163.481 kb on - strand, within MPMX20_00159 at 163.481 kb on - strand, within MPMX20_00159 at 163.481 kb on - strand, within MPMX20_00159 at 163.481 kb on - strand, within MPMX20_00159 at 163.481 kb on - strand, within MPMX20_00159 at 163.481 kb on - strand, within MPMX20_00159 at 163.509 kb on - strand, within MPMX20_00159 at 163.537 kb on - strand, within MPMX20_00159 at 163.541 kb on + strand, within MPMX20_00159 at 163.575 kb on - strand, within MPMX20_00159 at 163.705 kb on - strand, within MPMX20_00159 at 163.707 kb on + strand, within MPMX20_00159 at 163.707 kb on + strand, within MPMX20_00159 at 163.710 kb on - strand, within MPMX20_00159 at 163.715 kb on - strand, within MPMX20_00159 at 163.750 kb on + strand, within MPMX20_00159 at 163.796 kb on + strand, within MPMX20_00159 at 163.804 kb on - strand, within MPMX20_00159 at 163.806 kb on - strand, within MPMX20_00159 at 163.825 kb on - strand, within MPMX20_00159 at 163.879 kb on + strand, within MPMX20_00159 at 163.879 kb on + strand, within MPMX20_00159 at 163.879 kb on + strand, within MPMX20_00159 at 163.879 kb on - strand, within MPMX20_00159 at 163.910 kb on + strand, within MPMX20_00159 at 163.932 kb on + strand, within MPMX20_00159 at 163.997 kb on + strand, within MPMX20_00159 at 164.011 kb on + strand, within MPMX20_00159 at 164.057 kb on + strand, within MPMX20_00159 at 164.086 kb on + strand, within MPMX20_00159 at 164.086 kb on + strand, within MPMX20_00159 at 164.162 kb on - strand, within MPMX20_00159 at 164.162 kb on - strand, within MPMX20_00159 at 164.194 kb on - strand, within MPMX20_00159 at 164.220 kb on + strand, within MPMX20_00159 at 164.220 kb on + strand, within MPMX20_00159 at 164.228 kb on - strand, within MPMX20_00159 at 164.312 kb on - strand, within MPMX20_00159 at 164.394 kb on - strand at 164.407 kb on + strand at 164.415 kb on - strand at 164.422 kb on + strand at 164.431 kb on - strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.494 kb on + strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.502 kb on - strand at 164.582 kb on - strand at 164.585 kb on + strand at 164.585 kb on + strand at 164.585 kb on + strand at 164.717 kb on + strand at 164.717 kb on + strand at 164.717 kb on + strand at 164.717 kb on + strand at 164.717 kb on + strand at 164.717 kb on + strand at 164.717 kb on + strand at 164.725 kb on - strand at 164.725 kb on - strand at 164.725 kb on - strand at 164.725 kb on - strand at 164.725 kb on - strand at 164.725 kb on - strand at 164.757 kb on - strand at 164.816 kb on + strand at 164.826 kb on + strand at 164.834 kb on + strand at 164.842 kb on - strand at 164.842 kb on - strand at 164.842 kb on - strand at 164.862 kb on - strand at 164.881 kb on + strand at 164.894 kb on + strand at 164.924 kb on + strand at 164.951 kb on + strand, within MPMX20_00160 at 164.991 kb on + strand, within MPMX20_00160 at 164.991 kb on + strand, within MPMX20_00160 at 164.991 kb on + strand, within MPMX20_00160 at 164.991 kb on + strand, within MPMX20_00160 at 164.991 kb on + strand, within MPMX20_00160 at 164.991 kb on - strand, within MPMX20_00160 at 164.998 kb on + strand, within MPMX20_00160 at 164.998 kb on + strand, within MPMX20_00160 at 164.999 kb on - strand, within MPMX20_00160 at 164.999 kb on - strand, within MPMX20_00160 at 164.999 kb on - strand, within MPMX20_00160 at 164.999 kb on - strand, within MPMX20_00160 at 165.006 kb on + strand, within MPMX20_00160 at 165.006 kb on - strand, within MPMX20_00160 at 165.037 kb on + strand, within MPMX20_00160 at 165.045 kb on - strand, within MPMX20_00160 at 165.045 kb on - strand, within MPMX20_00160 at 165.045 kb on - strand, within MPMX20_00160 at 165.047 kb on + strand, within MPMX20_00160 at 165.105 kb on + strand, within MPMX20_00160 at 165.113 kb on - strand, within MPMX20_00160 at 165.113 kb on - strand, within MPMX20_00160 at 165.113 kb on - strand, within MPMX20_00160 at 165.209 kb on + strand, within MPMX20_00160 at 165.232 kb on + strand, within MPMX20_00160 at 165.281 kb on + strand, within MPMX20_00160 at 165.281 kb on + strand, within MPMX20_00160 at 165.281 kb on + strand, within MPMX20_00160 at 165.281 kb on + strand, within MPMX20_00160 at 165.281 kb on + strand, within MPMX20_00160 at 165.305 kb on - strand, within MPMX20_00160 at 165.310 kb on - strand, within MPMX20_00160 at 165.312 kb on + strand, within MPMX20_00160 at 165.352 kb on - strand, within MPMX20_00160 at 165.439 kb on + strand, within MPMX20_00160 at 165.442 kb on - strand, within MPMX20_00160 at 165.442 kb on - strand, within MPMX20_00160 at 165.449 kb on - strand, within MPMX20_00160 at 165.511 kb on - strand, within MPMX20_00160
Per-strain Table
Position Strand Gene LocusTag Fraction mutant library grown in Supernatant; Inquilinus sp. 005-A5 grown in LB remove 161,594 + MPMX20_00158 0.41 +0.1 161,594 + MPMX20_00158 0.41 -0.4 161,595 + MPMX20_00158 0.41 -1.2 161,668 + MPMX20_00158 0.47 -0.4 161,668 + MPMX20_00158 0.47 -1.0 161,668 + MPMX20_00158 0.47 -0.9 161,668 + MPMX20_00158 0.47 +0.4 161,676 - MPMX20_00158 0.48 -0.6 161,676 - MPMX20_00158 0.48 -3.7 161,676 - MPMX20_00158 0.48 -0.1 161,743 + MPMX20_00158 0.53 -2.4 161,784 + MPMX20_00158 0.56 -0.2 161,792 - MPMX20_00158 0.57 -0.5 161,792 - MPMX20_00158 0.57 +1.0 161,792 - MPMX20_00158 0.57 +0.7 161,816 + MPMX20_00158 0.59 +1.0 161,843 + MPMX20_00158 0.61 -0.3 161,865 - MPMX20_00158 0.63 -0.8 161,866 - MPMX20_00158 0.63 -0.1 161,888 - MPMX20_00158 0.65 +1.0 161,919 + MPMX20_00158 0.67 +0.1 161,970 + MPMX20_00158 0.71 -0.1 162,060 - MPMX20_00158 0.78 +0.8 162,065 - MPMX20_00158 0.79 -0.0 162,074 + MPMX20_00158 0.79 -0.3 162,082 - MPMX20_00158 0.80 -0.1 162,087 - MPMX20_00158 0.80 +0.2 162,103 + MPMX20_00158 0.82 -0.0 162,103 + MPMX20_00158 0.82 +0.0 162,106 - MPMX20_00158 0.82 +0.3 162,239 + +0.7 162,259 + +0.4 162,267 - +0.2 162,282 + +1.1 162,282 + +0.2 162,286 - -0.2 162,327 + +0.7 162,337 + -2.6 162,371 + -0.7 162,371 + +0.1 162,379 - -0.7 162,385 - -0.3 162,385 - +0.0 162,385 - -0.5 162,385 - -0.5 162,392 + -0.6 162,487 + +0.0 162,496 - -1.1 162,526 + +0.6 162,535 + -0.4 162,535 + +0.2 162,535 + -0.5 162,535 + +0.4 162,535 + -0.6 162,535 + -1.1 162,535 + -0.8 162,543 - +0.7 162,543 - -6.8 162,543 - +0.8 162,543 - -1.8 162,550 + +0.2 162,570 + -1.6 162,596 + -0.6 162,631 + -0.9 162,642 + +0.2 162,667 + -0.9 162,667 + +0.7 162,667 + -0.3 162,697 - +0.0 162,699 + -0.2 162,699 + -0.7 162,699 + -1.0 162,699 + +1.7 162,707 - -2.0 162,707 - +0.1 162,707 - +0.8 162,720 + MPMX20_00159 0.10 +1.7 162,728 + MPMX20_00159 0.11 +0.7 162,733 + MPMX20_00159 0.11 +0.1 162,736 - MPMX20_00159 0.11 +1.0 162,736 - MPMX20_00159 0.11 +0.4 162,743 + MPMX20_00159 0.11 +0.4 162,802 - MPMX20_00159 0.14 -0.9 162,807 - MPMX20_00159 0.14 -0.3 162,817 - MPMX20_00159 0.15 -1.3 162,837 + MPMX20_00159 0.16 +0.0 162,865 - MPMX20_00159 0.17 -1.4 162,875 - MPMX20_00159 0.18 -0.9 162,875 - MPMX20_00159 0.18 +0.1 162,917 + MPMX20_00159 0.20 +0.5 162,934 - MPMX20_00159 0.21 -0.3 162,983 + MPMX20_00159 0.23 +0.0 162,983 + MPMX20_00159 0.23 -0.5 163,055 + MPMX20_00159 0.27 -0.4 163,167 - MPMX20_00159 0.32 +1.2 163,191 + MPMX20_00159 0.33 +0.3 163,201 - MPMX20_00159 0.34 +0.2 163,231 - MPMX20_00159 0.35 +0.3 163,270 + MPMX20_00159 0.37 +0.0 163,271 - MPMX20_00159 0.37 +0.6 163,271 - MPMX20_00159 0.37 -1.6 163,278 - MPMX20_00159 0.38 +2.0 163,278 - MPMX20_00159 0.38 +0.8 163,285 + MPMX20_00159 0.38 +0.1 163,357 - MPMX20_00159 0.42 +0.0 163,357 - MPMX20_00159 0.42 -0.3 163,404 + MPMX20_00159 0.44 -0.7 163,450 + MPMX20_00159 0.46 +0.3 163,473 + MPMX20_00159 0.47 -1.1 163,473 + MPMX20_00159 0.47 +0.7 163,473 + MPMX20_00159 0.47 -0.0 163,473 + MPMX20_00159 0.47 +0.2 163,473 + MPMX20_00159 0.47 +0.6 163,481 - MPMX20_00159 0.48 -0.5 163,481 - MPMX20_00159 0.48 -1.0 163,481 - MPMX20_00159 0.48 +0.4 163,481 - MPMX20_00159 0.48 -0.0 163,481 - MPMX20_00159 0.48 -0.9 163,481 - MPMX20_00159 0.48 -0.0 163,481 - MPMX20_00159 0.48 -1.4 163,509 - MPMX20_00159 0.49 -0.3 163,537 - MPMX20_00159 0.50 -1.4 163,541 + MPMX20_00159 0.51 +0.3 163,575 - MPMX20_00159 0.52 -0.3 163,705 - MPMX20_00159 0.59 +0.8 163,707 + MPMX20_00159 0.59 -0.1 163,707 + MPMX20_00159 0.59 -1.0 163,710 - MPMX20_00159 0.59 -0.2 163,715 - MPMX20_00159 0.59 +0.1 163,750 + MPMX20_00159 0.61 +0.7 163,796 + MPMX20_00159 0.63 -0.4 163,804 - MPMX20_00159 0.64 +1.0 163,806 - MPMX20_00159 0.64 +1.3 163,825 - MPMX20_00159 0.65 +0.1 163,879 + MPMX20_00159 0.67 +0.7 163,879 + MPMX20_00159 0.67 +0.7 163,879 + MPMX20_00159 0.67 +0.6 163,879 - MPMX20_00159 0.67 +0.5 163,910 + MPMX20_00159 0.69 -1.1 163,932 + MPMX20_00159 0.70 +0.6 163,997 + MPMX20_00159 0.73 +0.1 164,011 + MPMX20_00159 0.74 +0.2 164,057 + MPMX20_00159 0.76 -0.6 164,086 + MPMX20_00159 0.77 +0.6 164,086 + MPMX20_00159 0.77 +0.3 164,162 - MPMX20_00159 0.81 -0.1 164,162 - MPMX20_00159 0.81 -0.2 164,194 - MPMX20_00159 0.83 -1.6 164,220 + MPMX20_00159 0.84 +0.3 164,220 + MPMX20_00159 0.84 +0.0 164,228 - MPMX20_00159 0.84 -0.2 164,312 - MPMX20_00159 0.89 -1.8 164,394 - -0.4 164,407 + +1.7 164,415 - -0.9 164,422 + -0.3 164,431 - -0.7 164,494 + +0.3 164,494 + -0.5 164,494 + +0.3 164,494 + +1.6 164,494 + -0.1 164,494 + -0.5 164,494 + +0.3 164,494 + -0.1 164,502 - -0.1 164,502 - -0.6 164,502 - +2.0 164,502 - +2.1 164,502 - +0.1 164,502 - +0.7 164,502 - -0.1 164,502 - -0.2 164,502 - -0.3 164,582 - -0.3 164,585 + -1.1 164,585 + +0.1 164,585 + +0.4 164,717 + +0.3 164,717 + +0.1 164,717 + +0.1 164,717 + +0.4 164,717 + -0.1 164,717 + +0.1 164,717 + +0.8 164,725 - -0.0 164,725 - +0.7 164,725 - +0.5 164,725 - +0.2 164,725 - +0.6 164,725 - +0.2 164,757 - +0.0 164,816 + -0.6 164,826 + -0.3 164,834 + +0.5 164,842 - -2.0 164,842 - -1.4 164,842 - +0.9 164,862 - -0.3 164,881 + -1.6 164,894 + -0.7 164,924 + -0.2 164,951 + MPMX20_00160 0.11 -0.1 164,991 + MPMX20_00160 0.16 -0.6 164,991 + MPMX20_00160 0.16 -0.0 164,991 + MPMX20_00160 0.16 +0.1 164,991 + MPMX20_00160 0.16 +2.3 164,991 + MPMX20_00160 0.16 -0.7 164,991 - MPMX20_00160 0.16 +2.0 164,998 + MPMX20_00160 0.17 +0.1 164,998 + MPMX20_00160 0.17 -0.3 164,999 - MPMX20_00160 0.17 +0.6 164,999 - MPMX20_00160 0.17 -1.5 164,999 - MPMX20_00160 0.17 -0.4 164,999 - MPMX20_00160 0.17 -1.3 165,006 + MPMX20_00160 0.18 +0.1 165,006 - MPMX20_00160 0.18 +0.5 165,037 + MPMX20_00160 0.22 -0.5 165,045 - MPMX20_00160 0.23 -1.7 165,045 - MPMX20_00160 0.23 -2.2 165,045 - MPMX20_00160 0.23 -0.7 165,047 + MPMX20_00160 0.23 +0.1 165,105 + MPMX20_00160 0.30 -0.4 165,113 - MPMX20_00160 0.31 -2.1 165,113 - MPMX20_00160 0.31 -0.7 165,113 - MPMX20_00160 0.31 -0.2 165,209 + MPMX20_00160 0.43 -0.3 165,232 + MPMX20_00160 0.45 -1.0 165,281 + MPMX20_00160 0.51 -1.9 165,281 + MPMX20_00160 0.51 -1.2 165,281 + MPMX20_00160 0.51 -1.1 165,281 + MPMX20_00160 0.51 +0.3 165,281 + MPMX20_00160 0.51 +0.3 165,305 - MPMX20_00160 0.54 -1.7 165,310 - MPMX20_00160 0.55 -0.9 165,312 + MPMX20_00160 0.55 +1.7 165,352 - MPMX20_00160 0.60 -0.4 165,439 + MPMX20_00160 0.70 +0.3 165,442 - MPMX20_00160 0.71 -1.1 165,442 - MPMX20_00160 0.71 -2.5 165,449 - MPMX20_00160 0.72 -0.5 165,511 - MPMX20_00160 0.79 -0.5
Or see this region's nucleotide sequence