Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS24695

Experiment: Control_Nissle

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS24685 and ECOLIN_RS24690 overlap by 1 nucleotidesECOLIN_RS24690 and ECOLIN_RS24695 are separated by 353 nucleotidesECOLIN_RS24695 and ECOLIN_RS33120 are separated by 153 nucleotidesECOLIN_RS33120 and ECOLIN_RS24700 are separated by 62 nucleotides ECOLIN_RS24685: ECOLIN_RS24685 - valine--tRNA ligase, at 4,964,678 to 4,967,533 _RS24685 ECOLIN_RS24690: ECOLIN_RS24690 - DNA polymerase III subunit chi, at 4,967,533 to 4,967,976 _RS24690 ECOLIN_RS24695: ECOLIN_RS24695 - leucyl aminopeptidase, at 4,968,330 to 4,969,841 _RS24695 ECOLIN_RS33120: ECOLIN_RS33120 - protein YtgA, at 4,969,995 to 4,970,045 _RS33120 ECOLIN_RS24700: ECOLIN_RS24700 - LPS export ABC transporter permease LptF, at 4,970,108 to 4,971,208 _RS24700 Position (kb) 4968 4969 4970Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 4967.686 kb on - strand, within ECOLIN_RS24690at 4967.692 kb on + strand, within ECOLIN_RS24690at 4967.958 kb on - strandat 4968.002 kb on - strandat 4968.006 kb on - strandat 4968.019 kb on - strandat 4968.053 kb on + strandat 4968.082 kb on + strandat 4968.082 kb on + strandat 4968.083 kb on - strandat 4968.161 kb on - strandat 4968.161 kb on - strandat 4968.179 kb on - strandat 4968.213 kb on + strandat 4968.247 kb on + strandat 4968.461 kb on - strandat 4968.461 kb on - strandat 4968.464 kb on + strandat 4968.465 kb on - strandat 4968.748 kb on + strand, within ECOLIN_RS24695at 4968.749 kb on - strand, within ECOLIN_RS24695at 4968.769 kb on - strand, within ECOLIN_RS24695at 4968.769 kb on - strand, within ECOLIN_RS24695at 4968.963 kb on - strand, within ECOLIN_RS24695at 4968.963 kb on - strand, within ECOLIN_RS24695at 4968.963 kb on - strand, within ECOLIN_RS24695at 4968.963 kb on - strand, within ECOLIN_RS24695at 4968.963 kb on - strand, within ECOLIN_RS24695at 4968.968 kb on - strand, within ECOLIN_RS24695at 4968.968 kb on - strand, within ECOLIN_RS24695at 4969.135 kb on + strand, within ECOLIN_RS24695at 4969.135 kb on + strand, within ECOLIN_RS24695at 4969.201 kb on + strand, within ECOLIN_RS24695at 4969.238 kb on - strand, within ECOLIN_RS24695at 4969.430 kb on - strand, within ECOLIN_RS24695at 4969.491 kb on - strand, within ECOLIN_RS24695at 4969.547 kb on + strand, within ECOLIN_RS24695at 4969.548 kb on - strand, within ECOLIN_RS24695at 4969.576 kb on + strand, within ECOLIN_RS24695at 4969.717 kb on + strandat 4969.832 kb on + strandat 4970.001 kb on - strand, within ECOLIN_RS33120at 4970.011 kb on + strand, within ECOLIN_RS33120

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_Nissle
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4,967,686 - ECOLIN_RS24690 0.34 -0.1
4,967,692 + ECOLIN_RS24690 0.36 +2.2
4,967,958 - +0.2
4,968,002 - -0.0
4,968,006 - -0.0
4,968,019 - -0.2
4,968,053 + -0.1
4,968,082 + +0.2
4,968,082 + -0.2
4,968,083 - +0.1
4,968,161 - -1.7
4,968,161 - -0.3
4,968,179 - +0.1
4,968,213 + -0.7
4,968,247 + +0.2
4,968,461 - -0.4
4,968,461 - -0.2
4,968,464 + -0.7
4,968,465 - +0.4
4,968,748 + ECOLIN_RS24695 0.28 +1.9
4,968,749 - ECOLIN_RS24695 0.28 +0.1
4,968,769 - ECOLIN_RS24695 0.29 -0.2
4,968,769 - ECOLIN_RS24695 0.29 -1.0
4,968,963 - ECOLIN_RS24695 0.42 +0.2
4,968,963 - ECOLIN_RS24695 0.42 -0.1
4,968,963 - ECOLIN_RS24695 0.42 +0.1
4,968,963 - ECOLIN_RS24695 0.42 -0.4
4,968,963 - ECOLIN_RS24695 0.42 -0.5
4,968,968 - ECOLIN_RS24695 0.42 -0.3
4,968,968 - ECOLIN_RS24695 0.42 -0.3
4,969,135 + ECOLIN_RS24695 0.53 +0.6
4,969,135 + ECOLIN_RS24695 0.53 +0.3
4,969,201 + ECOLIN_RS24695 0.58 +0.4
4,969,238 - ECOLIN_RS24695 0.60 +5.1
4,969,430 - ECOLIN_RS24695 0.73 -0.2
4,969,491 - ECOLIN_RS24695 0.77 +0.3
4,969,547 + ECOLIN_RS24695 0.80 +0.0
4,969,548 - ECOLIN_RS24695 0.81 -0.4
4,969,576 + ECOLIN_RS24695 0.82 -0.2
4,969,717 + -0.4
4,969,832 + +0.5
4,970,001 - ECOLIN_RS33120 0.12 -0.5
4,970,011 + ECOLIN_RS33120 0.31 +0.1

Or see this region's nucleotide sequence