Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS24415

Experiment: Control_Nissle

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS24410 and ECOLIN_RS24415 overlap by 4 nucleotidesECOLIN_RS24415 and ECOLIN_RS24420 are separated by 9 nucleotidesECOLIN_RS24420 and ECOLIN_RS24425 are separated by 9 nucleotides ECOLIN_RS24410: ECOLIN_RS24410 - FAD-binding protein, at 4,903,163 to 4,905,235 _RS24410 ECOLIN_RS24415: ECOLIN_RS24415 - acyl CoA:acetate/3-ketoacid CoA transferase, at 4,905,232 to 4,906,773 _RS24415 ECOLIN_RS24420: ECOLIN_RS24420 - enoyl-CoA hydratase/isomerase family protein, at 4,906,783 to 4,907,559 _RS24420 ECOLIN_RS24425: ECOLIN_RS24425 - sugar phosphate isomerase/epimerase family protein, at 4,907,569 to 4,908,411 _RS24425 Position (kb) 4905 4906 4907Strain fitness (log2 ratio) -2 -1 0 1 2at 4904.309 kb on - strand, within ECOLIN_RS24410at 4904.311 kb on - strand, within ECOLIN_RS24410at 4904.341 kb on + strand, within ECOLIN_RS24410at 4904.342 kb on - strand, within ECOLIN_RS24410at 4904.342 kb on - strand, within ECOLIN_RS24410at 4904.344 kb on - strand, within ECOLIN_RS24410at 4904.357 kb on + strand, within ECOLIN_RS24410at 4904.361 kb on + strand, within ECOLIN_RS24410at 4904.405 kb on + strand, within ECOLIN_RS24410at 4904.405 kb on + strand, within ECOLIN_RS24410at 4904.832 kb on + strand, within ECOLIN_RS24410at 4905.010 kb on + strand, within ECOLIN_RS24410at 4905.010 kb on + strand, within ECOLIN_RS24410at 4905.010 kb on + strand, within ECOLIN_RS24410at 4905.010 kb on + strand, within ECOLIN_RS24410at 4905.011 kb on - strand, within ECOLIN_RS24410at 4905.011 kb on - strand, within ECOLIN_RS24410at 4905.012 kb on + strand, within ECOLIN_RS24410at 4905.013 kb on - strand, within ECOLIN_RS24410at 4905.013 kb on - strand, within ECOLIN_RS24410at 4905.013 kb on - strand, within ECOLIN_RS24410at 4905.013 kb on - strand, within ECOLIN_RS24410at 4905.013 kb on - strand, within ECOLIN_RS24410at 4905.013 kb on - strand, within ECOLIN_RS24410at 4905.013 kb on - strand, within ECOLIN_RS24410at 4905.013 kb on - strand, within ECOLIN_RS24410at 4905.013 kb on - strand, within ECOLIN_RS24410at 4905.013 kb on - strand, within ECOLIN_RS24410at 4905.013 kb on - strand, within ECOLIN_RS24410at 4905.013 kb on - strand, within ECOLIN_RS24410at 4905.074 kb on + strandat 4905.191 kb on - strandat 4905.405 kb on - strand, within ECOLIN_RS24415at 4906.029 kb on - strand, within ECOLIN_RS24415at 4906.107 kb on + strand, within ECOLIN_RS24415at 4906.107 kb on + strand, within ECOLIN_RS24415at 4906.182 kb on - strand, within ECOLIN_RS24415at 4906.260 kb on - strand, within ECOLIN_RS24415at 4906.318 kb on + strand, within ECOLIN_RS24415at 4906.613 kb on - strand, within ECOLIN_RS24415at 4906.613 kb on - strand, within ECOLIN_RS24415at 4906.767 kb on + strandat 4906.767 kb on + strandat 4906.768 kb on - strandat 4906.768 kb on - strandat 4906.774 kb on - strandat 4906.774 kb on - strandat 4906.900 kb on - strand, within ECOLIN_RS24420at 4906.926 kb on + strand, within ECOLIN_RS24420at 4906.929 kb on + strand, within ECOLIN_RS24420at 4907.330 kb on - strand, within ECOLIN_RS24420at 4907.413 kb on + strand, within ECOLIN_RS24420at 4907.577 kb on - strandat 4907.617 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_Nissle
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4,904,309 - ECOLIN_RS24410 0.55 +0.0
4,904,311 - ECOLIN_RS24410 0.55 +1.2
4,904,341 + ECOLIN_RS24410 0.57 -0.9
4,904,342 - ECOLIN_RS24410 0.57 +0.0
4,904,342 - ECOLIN_RS24410 0.57 -2.0
4,904,344 - ECOLIN_RS24410 0.57 +1.3
4,904,357 + ECOLIN_RS24410 0.58 -0.1
4,904,361 + ECOLIN_RS24410 0.58 +0.2
4,904,405 + ECOLIN_RS24410 0.60 +0.0
4,904,405 + ECOLIN_RS24410 0.60 +0.6
4,904,832 + ECOLIN_RS24410 0.81 +2.6
4,905,010 + ECOLIN_RS24410 0.89 +1.8
4,905,010 + ECOLIN_RS24410 0.89 +1.8
4,905,010 + ECOLIN_RS24410 0.89 +0.1
4,905,010 + ECOLIN_RS24410 0.89 +1.2
4,905,011 - ECOLIN_RS24410 0.89 +0.1
4,905,011 - ECOLIN_RS24410 0.89 +0.4
4,905,012 + ECOLIN_RS24410 0.89 +0.0
4,905,013 - ECOLIN_RS24410 0.89 -0.3
4,905,013 - ECOLIN_RS24410 0.89 +0.4
4,905,013 - ECOLIN_RS24410 0.89 +0.3
4,905,013 - ECOLIN_RS24410 0.89 -1.5
4,905,013 - ECOLIN_RS24410 0.89 +0.5
4,905,013 - ECOLIN_RS24410 0.89 -0.0
4,905,013 - ECOLIN_RS24410 0.89 +0.2
4,905,013 - ECOLIN_RS24410 0.89 +2.3
4,905,013 - ECOLIN_RS24410 0.89 -0.6
4,905,013 - ECOLIN_RS24410 0.89 +0.1
4,905,013 - ECOLIN_RS24410 0.89 -0.0
4,905,013 - ECOLIN_RS24410 0.89 +0.0
4,905,074 + +0.6
4,905,191 - -0.0
4,905,405 - ECOLIN_RS24415 0.11 +0.0
4,906,029 - ECOLIN_RS24415 0.52 +0.2
4,906,107 + ECOLIN_RS24415 0.57 +0.4
4,906,107 + ECOLIN_RS24415 0.57 +2.7
4,906,182 - ECOLIN_RS24415 0.62 -0.3
4,906,260 - ECOLIN_RS24415 0.67 -0.4
4,906,318 + ECOLIN_RS24415 0.70 -0.6
4,906,613 - ECOLIN_RS24415 0.90 -0.6
4,906,613 - ECOLIN_RS24415 0.90 -0.1
4,906,767 + +0.6
4,906,767 + +0.1
4,906,768 - -0.3
4,906,768 - -0.1
4,906,774 - +0.2
4,906,774 - +0.0
4,906,900 - ECOLIN_RS24420 0.15 +0.2
4,906,926 + ECOLIN_RS24420 0.18 +0.3
4,906,929 + ECOLIN_RS24420 0.19 +0.4
4,907,330 - ECOLIN_RS24420 0.70 -0.5
4,907,413 + ECOLIN_RS24420 0.81 -0.2
4,907,577 - -0.2
4,907,617 - +0.1

Or see this region's nucleotide sequence