Experiment: Control_Nissle
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS10745 and ECOLIN_RS10750 are separated by 10 nucleotides ECOLIN_RS10750 and ECOLIN_RS10755 are separated by 14 nucleotides ECOLIN_RS10755 and ECOLIN_RS10760 are separated by 2 nucleotides ECOLIN_RS10760 and ECOLIN_RS32340 are separated by 114 nucleotides
ECOLIN_RS10745: ECOLIN_RS10745 - protein phosphatase CheZ, at 2,093,384 to 2,094,028
_RS10745
ECOLIN_RS10750: ECOLIN_RS10750 - chemotaxis response regulator CheY, at 2,094,039 to 2,094,428
_RS10750
ECOLIN_RS10755: ECOLIN_RS10755 - protein-glutamate methylesterase/protein glutamine deamidase, at 2,094,443 to 2,095,492
_RS10755
ECOLIN_RS10760: ECOLIN_RS10760 - protein-glutamate O-methyltransferase CheR, at 2,095,495 to 2,096,355
_RS10760
ECOLIN_RS32340: ECOLIN_RS32340 - Tar ligand binding domain-containing protein, at 2,096,470 to 2,096,600
_RS32340
Position (kb) 2094
2095
2096 Strain fitness (log2 ratio) -1
0
1 at 2094.449 kb on - strand at 2094.817 kb on - strand, within ECOLIN_RS10755 at 2094.931 kb on + strand, within ECOLIN_RS10755 at 2096.320 kb on + strand at 2096.355 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_Nissle remove 2,094,449 - -0.6 2,094,817 - ECOLIN_RS10755 0.36 -1.1 2,094,931 + ECOLIN_RS10755 0.46 -1.1 2,096,320 + +0.2 2,096,355 + +0.8
Or see this region's nucleotide sequence