Experiment: Control_Nissle
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS02120 and ECOLIN_RS32670 are separated by 215 nucleotides ECOLIN_RS32670 and ECOLIN_RS02125 are separated by 86 nucleotides ECOLIN_RS02125 and ECOLIN_RS27735 are separated by 621 nucleotides ECOLIN_RS27735 and ECOLIN_RS02135 are separated by 16 nucleotides
ECOLIN_RS02120: ECOLIN_RS02120 - amidohydrolase family protein, at 430,740 to 432,122
_RS02120
ECOLIN_RS32670: ECOLIN_RS32670 - hypothetical protein, at 432,338 to 432,412
_RS32670
ECOLIN_RS02125: ECOLIN_RS02125 - NADPH-dependent aldehyde reductase YahK, at 432,499 to 433,548
_RS02125
ECOLIN_RS27735: ECOLIN_RS27735 - hypothetical protein, at 434,170 to 434,413
_RS27735
ECOLIN_RS02135: ECOLIN_RS02135 - LysE family transporter, at 434,430 to 435,104
_RS02135
Position (kb)
432
433
434 Strain fitness (log2 ratio)
-2
-1
0
1 at 431.605 kb on - strand, within ECOLIN_RS02120 at 431.607 kb on - strand, within ECOLIN_RS02120 at 431.899 kb on - strand, within ECOLIN_RS02120 at 431.956 kb on + strand, within ECOLIN_RS02120 at 431.957 kb on - strand, within ECOLIN_RS02120 at 432.131 kb on + strand at 432.131 kb on + strand at 432.164 kb on + strand at 432.309 kb on - strand at 432.330 kb on + strand at 432.331 kb on - strand at 432.331 kb on - strand at 432.331 kb on - strand at 432.471 kb on + strand at 432.477 kb on + strand at 432.477 kb on + strand at 432.477 kb on + strand at 433.072 kb on - strand, within ECOLIN_RS02125 at 433.111 kb on - strand, within ECOLIN_RS02125 at 433.446 kb on - strand at 433.697 kb on + strand at 434.075 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Control_Nissle remove 431,605 - ECOLIN_RS02120 0.63 +0.5 431,607 - ECOLIN_RS02120 0.63 +1.2 431,899 - ECOLIN_RS02120 0.84 -0.8 431,956 + ECOLIN_RS02120 0.88 +0.3 431,957 - ECOLIN_RS02120 0.88 +0.4 432,131 + +0.1 432,131 + -0.2 432,164 + -0.9 432,309 - +0.0 432,330 + +0.3 432,331 - -2.0 432,331 - +0.3 432,331 - +0.3 432,471 + -0.8 432,477 + -1.1 432,477 + -0.7 432,477 + +0.7 433,072 - ECOLIN_RS02125 0.55 -0.6 433,111 - ECOLIN_RS02125 0.58 -0.6 433,446 - +1.1 433,697 + -0.7 434,075 + +0.1
Or see this region's nucleotide sequence